Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C1929
  Reference Plasmid   1111525849668648_bin.8__k141_63399
  Reference Plasmid Size   8684
  Reference Plasmid GC Content   0.61
  Reference Plasmid Mobility Type   non-mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0020113 EAKDLBEI_00004 4402 7 Skin 0.50 protein_coding synonymous_variant LOW 681A>G Arg227Arg
M0020114 EAKDLBEI_00004 4408 6 Skin 0.43 protein_coding synonymous_variant LOW 675C>T Asp225Asp
M0020115 EAKDLBEI_00004 4420 6 Skin 0.43 protein_coding synonymous_variant LOW 663T>C Val221Val
M0020116 EAKDLBEI_00004 4426 6 Skin 0.43 protein_coding synonymous_variant LOW 657C>G Val219Val
M0020117 EAKDLBEI_00004 4429 6 Skin 0.43 protein_coding synonymous_variant LOW 654C>G Gly218Gly
M0020118 EAKDLBEI_00004 4433 6 Skin 0.43 protein_coding missense_variant MODERATE 650G>A Gly217Asp
M0020119 EAKDLBEI_00004 6587 6 Skin 0.43 protein_coding upstream_gene_variant MODIFIER -1505C>T None
M0020120 EAKDLBEI_00004 6623 7 Skin 0.50 protein_coding upstream_gene_variant MODIFIER -1541T>C None
M0020121 EAKDLBEI_00004 6641 7 Skin 0.50 protein_coding upstream_gene_variant MODIFIER -1559A>T None
M0020122 EAKDLBEI_00007 6896 4 Skin 0.29 protein_coding missense_variant MODERATE 755A>G Gln252Arg
M0020123 EAKDLBEI_00007 6901 4 Skin 0.29 protein_coding synonymous_variant LOW 750A>T Gly250Gly
M0020124 EAKDLBEI_00007 6904 4 Skin 0.29 protein_coding synonymous_variant LOW 747G>A Ala249Ala
M0020125 EAKDLBEI_00007 6911 4 Skin 0.29 protein_coding missense_variant MODERATE 740T>C Val247Ala
M0020126 EAKDLBEI_00007 6933 4 Skin 0.29 protein_coding missense_variant MODERATE 718G>A Ala240Thr
M0020127 EAKDLBEI_00007 6954 4 Skin 0.29 protein_coding missense_variant MODERATE 697A>G Lys233Glu
M0020128 EAKDLBEI_00007 6988 4 Skin 0.29 protein_coding synonymous_variant LOW 663G>A Val221Val
M0020129 EAKDLBEI_00007 7030 4 Skin 0.29 protein_coding synonymous_variant LOW 621A>G Leu207Leu
M0020130 EAKDLBEI_00007 7067 4 Skin 0.29 protein_coding missense_variant MODERATE 584G>C Gly195Ala
M0020131 EAKDLBEI_00007 7072 4 Skin 0.29 protein_coding synonymous_variant LOW 579A>G Ala193Ala
M0020132 EAKDLBEI_00007 7188 4 Skin 0.29 protein_coding missense_variant MODERATE 463C>G Leu155Val
M0020133 EAKDLBEI_00007 7221 5 Skin 0.36 protein_coding missense_variant MODERATE 430C>G Gln144Glu
M0020134 EAKDLBEI_00007 7228 5 Skin 0.36 protein_coding synonymous_variant LOW 423G>C Ala141Ala
M0020135 EAKDLBEI_00007 7263 4 Skin 0.29 protein_coding missense_variant MODERATE 388G>A Ala130Thr
M0020136 EAKDLBEI_00007 7318 6 Skin 0.43 protein_coding synonymous_variant LOW 333A>G Leu111Leu
M0020137 EAKDLBEI_00007 7372 8 Skin 0.57 protein_coding synonymous_variant LOW 279T>C Leu93Leu
M0020138 EAKDLBEI_00007 7381 8 Skin 0.57 protein_coding missense_variant MODERATE 270A>C Arg90Ser
M0020139 EAKDLBEI_00007 7462 5 Skin 0.36 protein_coding synonymous_variant LOW 189G>T Leu63Leu
M0020140 EAKDLBEI_00007 7517 5 Skin 0.36 protein_coding missense_variant MODERATE 134T>G Leu45Arg
M0020141 EAKDLBEI_00007 7561 5 Skin 0.36 protein_coding synonymous_variant LOW 90T>C His30His
M0020142 EAKDLBEI_00007 7573 5 Skin 0.36 protein_coding synonymous_variant LOW 78G>A Arg26Arg
M0020143 EAKDLBEI_00007 7606 4 Skin 0.29 protein_coding synonymous_variant LOW 45C>T Ile15Ile
M0020144 EAKDLBEI_00007 7636 5 Skin 0.36 protein_coding synonymous_variant LOW 15T>C Ala5Ala
M0020145 EAKDLBEI_00004 7777 5 Skin 0.36 protein_coding upstream_gene_variant MODIFIER -2695T>G None
M0020146 EAKDLBEI_00004 7830 5 Skin 0.36 protein_coding upstream_gene_variant MODIFIER -2748C>A None
M0020147 EAKDLBEI_00008 7960 5 Skin 0.36 protein_coding synonymous_variant LOW 102G>A Glu34Glu
M0020148 EAKDLBEI_00008 7963 5 Skin 0.36 protein_coding synonymous_variant LOW 105T>C Gly35Gly
M0020149 EAKDLBEI_00008 8048 4 Skin 0.29 protein_coding missense_variant MODERATE 190C>T Pro64Ser
M0020150 EAKDLBEI_00008 8101 4 Skin 0.29 protein_coding synonymous_variant LOW 243G>C Arg81Arg
M0020151 EAKDLBEI_00004 4639 7 Skin 0.50 protein_coding synonymous_variant LOW 444A>G Gln148Gln
M0020152 EAKDLBEI_00005 5591 6 Skin 0.43 protein_coding missense_variant MODERATE 160T>A Trp54Arg
M0020153 EAKDLBEI_00006 6218 4 Skin 0.29 protein_coding synonymous_variant LOW 324C>T Ile108Ile
M0020154 EAKDLBEI_00006 6395 5 Skin 0.36 protein_coding synonymous_variant LOW 147T>C Tyr49Tyr
M0020155 EAKDLBEI_00006 6419 5 Skin 0.36 protein_coding synonymous_variant LOW 123C>T Leu41Leu
M0020156 EAKDLBEI_00004 8555 8 Skin 0.57 protein_coding upstream_gene_variant MODIFIER -3473G>C None
M0020157 EAKDLBEI_00004 8621 8 Skin 0.57 protein_coding upstream_gene_variant MODIFIER -3539C>G None
M0020158 EAKDLBEI_00008 8438 4 Skin 0.29 protein_coding missense_variant MODERATE 580A>G Ile194Val
M0020159 EAKDLBEI_00004 8553 3 Skin 0.21 protein_coding upstream_gene_variant MODIFIER -3471A>G None
M0020160 EAKDLBEI_00004 8562 3 Skin 0.21 protein_coding upstream_gene_variant MODIFIER -3480T>C None
M0020161 EAKDLBEI_00004 8589 3 Skin 0.21 protein_coding upstream_gene_variant MODIFIER -3507G>A None
M0020162 EAKDLBEI_00004 8597 3 Skin 0.21 protein_coding upstream_gene_variant MODIFIER -3515G>C None
M0020163 EAKDLBEI_00004 8637 3 Skin 0.21 protein_coding upstream_gene_variant MODIFIER -3555C>T None
M0020164 EAKDLBEI_00004 8652 3 Skin 0.21 protein_coding upstream_gene_variant MODIFIER -3570C>T None
M0020165 EAKDLBEI_00005 5406 3 Skin 0.21 protein_coding synonymous_variant LOW 345T>C Ile115Ile
M0020166 EAKDLBEI_00006 5916 3 Skin 0.21 protein_coding missense_variant MODERATE 626G>A Arg209His
M0020167 EAKDLBEI_00004 6545 4 Skin 0.29 protein_coding upstream_gene_variant MODIFIER -1463T>C None
M0020168 EAKDLBEI_00004 4864 3 Skin 0.21 protein_coding synonymous_variant LOW 219G>A Ala73Ala






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
EAKDLBEI_00004 3.A.1.3.10 75.5 6.1e-100 2 246 0.9722 0.9800 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.1 The ATP-binding Cassette (ABC) Superfamily
EAKDLBEI_00005 3.A.1.3.10 72.7 1.4e-84 2 221 0.9910 0.8800 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.1 The ATP-binding Cassette (ABC) Superfamily