Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C2012
  Reference Plasmid   1111525849736936_bin.43__k141_189536
  Reference Plasmid Size   25407
  Reference Plasmid GC Content   0.36
  Reference Plasmid Mobility Type   non-mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0022338 AEMLCJDH_00002 1043 10 Skin 0.29 protein_coding missense_variant MODERATE 658G>A Gly220Arg
M0022339 AEMLCJDH_00002 1050 8 Skin 0.24 protein_coding synonymous_variant LOW 651T>C Pro217Pro
M0022340 AEMLCJDH_00003 1936 14 Skin 0.41 protein_coding synonymous_variant LOW 1236C>T Asn412Asn
M0022341 AEMLCJDH_00003 2437 4 Skin 0.12 protein_coding synonymous_variant LOW 735G>A Lys245Lys
M0022342 AEMLCJDH_00006 197 4 Skin 0.12 protein_coding upstream_gene_variant MODIFIER -4470G>A None
M0022343 AEMLCJDH_00006 206 6 Skin 0.18 protein_coding upstream_gene_variant MODIFIER -4461T>C None
M0022344 AEMLCJDH_00006 260 8 Skin 0.24 protein_coding upstream_gene_variant MODIFIER -4407T>G None
M0022345 AEMLCJDH_00001 377 6 Skin 0.18 protein_coding synonymous_variant LOW 324C>T Asn108Asn
M0022346 AEMLCJDH_00001 464 5 Skin 0.15 protein_coding synonymous_variant LOW 237G>A Val79Val
M0022347 AEMLCJDH_00003 2719 10 Skin 0.29 protein_coding synonymous_variant LOW 453T>C Arg151Arg
M0022348 AEMLCJDH_00003 2917 5 Skin 0.15 protein_coding synonymous_variant LOW 255G>C Val85Val
M0022349 AEMLCJDH_00001 3202 7 Skin 0.21 protein_coding upstream_gene_variant MODIFIER -2502C>T None
M0022350 AEMLCJDH_00004 3487 3 Skin 0.09 protein_coding synonymous_variant LOW 837T>C Asp279Asp
M0022351 AEMLCJDH_00004 3540 3 Skin 0.09 protein_coding missense_variant MODERATE 784G>A Ala262Thr
M0022352 AEMLCJDH_00008 5985 3 Skin 0.09 protein_coding synonymous_variant LOW 126G>A Lys42Lys
M0022353 AEMLCJDH_00008 6000 3 Skin 0.09 protein_coding synonymous_variant LOW 141T>C Ala47Ala
M0022354 AEMLCJDH_00008 6375 11 Skin 0.32 protein_coding synonymous_variant LOW 516T>C Pro172Pro
M0022355 AEMLCJDH_00008 6386 6 Skin 0.18 protein_coding missense_variant MODERATE 527A>C Lys176Thr
M0022356 AEMLCJDH_00008 6498 6 Skin 0.18 protein_coding synonymous_variant LOW 639C>T Ile213Ile
M0022357 AEMLCJDH_00009 6774 5 Skin 0.15 protein_coding synonymous_variant LOW 2289C>T Phe763Phe
M0022358 AEMLCJDH_00009 8328 11 Skin 0.32 protein_coding synonymous_variant LOW 735G>C Thr245Thr
M0022359 AEMLCJDH_00011 10396 6 Skin 0.18 protein_coding missense_variant MODERATE 841A>G Ile281Val
M0022360 AEMLCJDH_00011 10892 12 Skin 0.35 protein_coding missense_variant MODERATE 1337A>G Asp446Gly
M0022361 AEMLCJDH_00009 11499 8 Skin 0.24 protein_coding upstream_gene_variant MODIFIER -2437T>C None
M0022362 AEMLCJDH_00012 11672 9 Skin 0.26 protein_coding synonymous_variant LOW 90G>A Lys30Lys
M0022363 AEMLCJDH_00012 11874 9 Skin 0.26 protein_coding synonymous_variant LOW 292T>C Leu98Leu
M0022364 AEMLCJDH_00012 11957 9 Skin 0.26 protein_coding synonymous_variant LOW 375G>A Gln125Gln
M0022365 AEMLCJDH_00009 12112 9 Skin 0.26 protein_coding upstream_gene_variant MODIFIER -3050G>A None
M0022366 AEMLCJDH_00009 12349 3 Skin 0.09 protein_coding upstream_gene_variant MODIFIER -3287C>T None
M0022367 AEMLCJDH_00016 14491 5 Skin 0.15 protein_coding synonymous_variant LOW 96C>T His32His
M0022368 AEMLCJDH_00016 14494 5 Skin 0.15 protein_coding synonymous_variant LOW 99C>T Thr33Thr
M0022369 AEMLCJDH_00016 14650 5 Skin 0.15 protein_coding synonymous_variant LOW 255C>T Ser85Ser
M0022370 AEMLCJDH_00016 14713 5 Skin 0.15 protein_coding synonymous_variant LOW 318A>G Gly106Gly
M0022371 AEMLCJDH_00016 14758 6 Skin 0.18 protein_coding synonymous_variant LOW 363T>C Asn121Asn
M0022372 AEMLCJDH_00016 14825 9 Skin 0.26 protein_coding missense_variant MODERATE 430T>G Phe144Val
M0022373 AEMLCJDH_00016 14831 9 Skin 0.26 protein_coding missense_variant MODERATE 436A>G Asn146Asp
M0022374 AEMLCJDH_00016 14833 9 Skin 0.26 protein_coding synonymous_variant LOW 438C>T Asn146Asn
M0022375 AEMLCJDH_00016 14836 9 Skin 0.26 protein_coding synonymous_variant LOW 441T>C Gly147Gly
M0022376 AEMLCJDH_00016 14839 9 Skin 0.26 protein_coding synonymous_variant LOW 444G>A Lys148Lys
M0022377 AEMLCJDH_00018 15343 8 Skin 0.24 protein_coding synonymous_variant LOW 102A>G Pro34Pro
M0022378 AEMLCJDH_00018 15344 8 Skin 0.24 protein_coding missense_variant MODERATE 103A>C Lys35Gln
M0022379 AEMLCJDH_00018 15346 8 Skin 0.24 protein_coding synonymous_variant LOW 105A>G Lys35Lys
M0022380 AEMLCJDH_00018 15426 8 Skin 0.24 protein_coding missense_variant MODERATE 185A>C Asp62Ala
M0022381 AEMLCJDH_00018 15511 7 Skin 0.21 protein_coding synonymous_variant LOW 270G>A Val90Val
M0022382 AEMLCJDH_00018 15561 8 Skin 0.24 protein_coding missense_variant MODERATE 320T>C Val107Ala
M0022383 AEMLCJDH_00019 15692 9 Skin 0.26 protein_coding missense_variant MODERATE 91A>G Asn31Asp
M0022384 AEMLCJDH_00019 15928 7 Skin 0.21 protein_coding synonymous_variant LOW 327A>T Ser109Ser
M0022385 AEMLCJDH_00014 16084 7 Skin 0.21 protein_coding upstream_gene_variant MODIFIER -2354T>C None
M0022386 AEMLCJDH_00014 16393 5 Skin 0.15 protein_coding upstream_gene_variant MODIFIER -2663A>G None
M0022387 AEMLCJDH_00014 16396 5 Skin 0.15 protein_coding upstream_gene_variant MODIFIER -2666T>C None
M0022388 AEMLCJDH_00020 16934 6 Skin 0.18 protein_coding missense_variant MODERATE 520A>G Thr174Ala
M0022389 AEMLCJDH_00020 17212 7 Skin 0.21 protein_coding synonymous_variant LOW 798G>A Glu266Glu
M0022390 AEMLCJDH_00020 17252 6 Skin 0.18 protein_coding missense_variant MODERATE 838T>C Phe280Leu
M0022391 AEMLCJDH_00009 7476 8 Skin 0.24 protein_coding synonymous_variant LOW 1587C>T Gly529Gly
M0022392 AEMLCJDH_00009 7574 3 Skin 0.09 protein_coding missense_variant MODERATE 1489C>T Pro497Ser
M0022393 AEMLCJDH_00009 7848 6 Skin 0.18 protein_coding synonymous_variant LOW 1215C>T Asn405Asn
M0022394 AEMLCJDH_00011 9975 9 Skin 0.26 protein_coding synonymous_variant LOW 420C>T Asp140Asp
M0022395 AEMLCJDH_00013 12622 3 Skin 0.09 protein_coding missense_variant MODERATE 194A>G Lys65Arg
M0022396 AEMLCJDH_00009 13089 3 Skin 0.09 protein_coding upstream_gene_variant MODIFIER -4027A>G None
M0022397 AEMLCJDH_00014 13238 3 Skin 0.09 protein_coding missense_variant MODERATE 493G>A Glu165Lys
M0022398 AEMLCJDH_00014 13338 6 Skin 0.18 protein_coding synonymous_variant LOW 393C>T His131His
M0022399 AEMLCJDH_00009 13755 3 Skin 0.09 protein_coding upstream_gene_variant MODIFIER -4693C>A None
M0022400 AEMLCJDH_00015 14256 7 Skin 0.21 protein_coding synonymous_variant LOW 210G>A Gln70Gln
M0022401 AEMLCJDH_00015 14260 7 Skin 0.21 protein_coding missense_variant MODERATE 214T>C Phe72Leu
M0022402 AEMLCJDH_00015 14264 7 Skin 0.21 protein_coding missense_variant MODERATE 218G>A Ser73Asn
M0022403 AEMLCJDH_00014 14291 7 Skin 0.21 protein_coding upstream_gene_variant MODIFIER -561C>T None
M0022404 AEMLCJDH_00014 14303 7 Skin 0.21 protein_coding upstream_gene_variant MODIFIER -573G>A None
M0022405 AEMLCJDH_00014 14304 7 Skin 0.21 protein_coding upstream_gene_variant MODIFIER -574C>T None
M0022406 AEMLCJDH_00014 14305 7 Skin 0.21 protein_coding upstream_gene_variant MODIFIER -575A>G None
M0022407 AEMLCJDH_00014 14306 7 Skin 0.21 protein_coding upstream_gene_variant MODIFIER -576C>G None
M0022408 AEMLCJDH_00014 14316 7 Skin 0.21 protein_coding upstream_gene_variant MODIFIER -586T>C None
M0022409 AEMLCJDH_00014 14326 7 Skin 0.21 protein_coding upstream_gene_variant MODIFIER -596A>G None
M0022410 AEMLCJDH_00014 14365 6 Skin 0.18 protein_coding upstream_gene_variant MODIFIER -635C>T None
M0022411 AEMLCJDH_00014 14377 6 Skin 0.18 protein_coding upstream_gene_variant MODIFIER -647C>A None
M0022412 AEMLCJDH_00014 14380 6 Skin 0.18 protein_coding upstream_gene_variant MODIFIER -650A>G None
M0022413 AEMLCJDH_00002 1252 3 Skin 0.09 protein_coding missense_variant MODERATE 449G>A Gly150Asp
M0022414 AEMLCJDH_00002 1499 3 Skin 0.09 protein_coding missense_variant MODERATE 202G>T Ala68Ser
M0022415 AEMLCJDH_00003 3034 5 Skin 0.15 protein_coding synonymous_variant LOW 138T>C Asn46Asn
M0022416 AEMLCJDH_00006 5057 5 Skin 0.15 protein_coding missense_variant MODERATE 391G>A Gly131Ser
M0022417 AEMLCJDH_00006 5384 4 Skin 0.12 protein_coding missense_variant MODERATE 718A>G Thr240Ala
M0022418 AEMLCJDH_00009 7563 4 Skin 0.12 protein_coding synonymous_variant LOW 1500A>G Lys500Lys
M0022419 AEMLCJDH_00009 9002 6 Skin 0.18 protein_coding missense_variant MODERATE 61G>A Gly21Ser
M0022420 AEMLCJDH_00004 9228 6 Skin 0.18 protein_coding upstream_gene_variant MODIFIER -4905G>A None
M0022421 AEMLCJDH_00010 9294 6 Skin 0.18 protein_coding missense_variant MODERATE 35A>G Asn12Ser
M0022422 AEMLCJDH_00010 9304 6 Skin 0.18 protein_coding synonymous_variant LOW 45C>T Pro15Pro
M0022423 AEMLCJDH_00010 9346 6 Skin 0.18 protein_coding synonymous_variant LOW 87T>C Phe29Phe
M0022424 AEMLCJDH_00005 9477 6 Skin 0.18 protein_coding upstream_gene_variant MODIFIER -4981T>A None
M0022425 AEMLCJDH_00011 10542 3 Skin 0.09 protein_coding synonymous_variant LOW 987C>T Ile329Ile
M0022426 AEMLCJDH_00015 14064 3 Skin 0.09 protein_coding synonymous_variant LOW 18C>T Ile6Ile
M0022427 AEMLCJDH_00015 14067 3 Skin 0.09 protein_coding synonymous_variant LOW 21C>T Asp7Asp
M0022428 AEMLCJDH_00015 14124 3 Skin 0.09 protein_coding synonymous_variant LOW 78G>C Val26Val
M0022429 AEMLCJDH_00014 14302 3 Skin 0.09 protein_coding upstream_gene_variant MODIFIER -572T>C None
M0022430 AEMLCJDH_00016 14575 3 Skin 0.09 protein_coding synonymous_variant LOW 180T>C Asp60Asp
M0022431 AEMLCJDH_00018 15299 3 Skin 0.09 protein_coding missense_variant MODERATE 58A>G Ser20Gly
M0022432 AEMLCJDH_00023 19764 6 Skin 0.18 protein_coding missense_variant MODERATE 37G>A Asp13Asn
M0022433 AEMLCJDH_00025 21642 6 Skin 0.18 protein_coding upstream_gene_variant MODIFIER -726C>T None
M0022434 AEMLCJDH_00006 5303 4 Skin 0.12 protein_coding missense_variant MODERATE 637G>A Asp213Asn
M0022435 AEMLCJDH_00009 8146 3 Skin 0.09 protein_coding missense_variant MODERATE 917G>A Ser306Asn
M0022436 AEMLCJDH_00022 18229 6 Skin 0.18 protein_coding missense_variant MODERATE 275G>A Cys92Tyr
M0022437 AEMLCJDH_00022 18478 6 Skin 0.18 protein_coding missense_variant MODERATE 524T>C Val175Ala
M0022438 AEMLCJDH_00022 18525 6 Skin 0.18 protein_coding missense_variant MODERATE 571A>G Thr191Ala
M0022439 AEMLCJDH_00023 19426 6 Skin 0.18 protein_coding upstream_gene_variant MODIFIER -302G>A None
M0022440 AEMLCJDH_00025 23063 4 Skin 0.12 protein_coding upstream_gene_variant MODIFIER -2147G>A None
M0022441 AEMLCJDH_00025 24119 4 Skin 0.12 protein_coding upstream_gene_variant MODIFIER -3203C>T None
M0022442 AEMLCJDH_00022 18038 4 Skin 0.12 protein_coding synonymous_variant LOW 84C>T Gly28Gly
M0022443 AEMLCJDH_00022 18646 3 Skin 0.09 protein_coding missense_variant MODERATE 692C>A Ser231Tyr
M0022444 AEMLCJDH_00006 5479 3 Skin 0.09 protein_coding synonymous_variant LOW 813C>T Thr271Thr
M0022445 AEMLCJDH_00008 5935 6 Skin 0.18 protein_coding missense_variant MODERATE 76G>A Val26Ile
M0022446 AEMLCJDH_00008 5982 3 Skin 0.09 protein_coding synonymous_variant LOW 123T>C Gly41Gly
M0022447 AEMLCJDH_00025 25266 3 Skin 0.09 protein_coding upstream_gene_variant MODIFIER -4350A>G None
M0022448 AEMLCJDH_00006 5182 3 Skin 0.09 protein_coding synonymous_variant LOW 516G>A Thr172Thr
M0022449 AEMLCJDH_00006 5503 3 Skin 0.09 protein_coding synonymous_variant LOW 837C>T Cys279Cys
M0022450 AEMLCJDH_00008 6154 3 Skin 0.09 protein_coding missense_variant MODERATE 295G>A Val99Met
M0022451 AEMLCJDH_00008 6156 3 Skin 0.09 protein_coding synonymous_variant LOW 297G>A Val99Val
M0022452 AEMLCJDH_00008 6168 3 Skin 0.09 protein_coding synonymous_variant LOW 309A>G Glu103Glu






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term