Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C2191
  Reference Plasmid   1111525849744228_bin.8__k141_4705
  Reference Plasmid Size   25606
  Reference Plasmid GC Content   0.62
  Reference Plasmid Mobility Type   non-mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0029311 IFGCFLCK_00007 6641 35 Skin 0.36 protein_coding synonymous_variant LOW 792A>G Ala264Ala
M0029312 IFGCFLCK_00007 6689 39 Skin 0.41 protein_coding synonymous_variant LOW 840A>G Glu280Glu
M0029313 IFGCFLCK_00007 6698 39 Skin 0.41 protein_coding synonymous_variant LOW 849T>G Val283Val
M0029314 IFGCFLCK_00009 11642 3 Skin 0.03 protein_coding missense_variant MODERATE 2350A>G Asn784Asp
M0029315 IFGCFLCK_00009 11646 3 Skin 0.03 protein_coding missense_variant MODERATE 2354T>C Val785Ala
M0029316 IFGCFLCK_00009 11648 3 Skin 0.03 protein_coding synonymous_variant LOW 2356C>A Arg786Arg
M0029317 IFGCFLCK_00009 11716 3 Skin 0.03 protein_coding synonymous_variant LOW 2424T>A Pro808Pro
M0029318 IFGCFLCK_00009 11770 5 Skin 0.05 protein_coding synonymous_variant LOW 2478G>A Pro826Pro
M0029319 IFGCFLCK_00009 11818 4 Skin 0.04 protein_coding synonymous_variant LOW 2526A>G Ala842Ala
M0029320 IFGCFLCK_00009 11823 4 Skin 0.04 protein_coding missense_variant MODERATE 2531T>C Val844Ala
M0029321 IFGCFLCK_00009 11838 4 Skin 0.04 protein_coding missense_variant MODERATE 2546A>C Glu849Ala
M0029322 IFGCFLCK_00009 11839 4 Skin 0.04 protein_coding missense_variant MODERATE 2547A>T Glu849Asp
M0029323 IFGCFLCK_00009 11854 4 Skin 0.04 protein_coding synonymous_variant LOW 2562C>T Ala854Ala
M0029324 IFGCFLCK_00009 11875 4 Skin 0.04 protein_coding synonymous_variant LOW 2583C>T Ile861Ile
M0029325 IFGCFLCK_00008 7489 4 Skin 0.04 protein_coding synonymous_variant LOW 1287T>C His429His
M0029326 IFGCFLCK_00009 12211 5 Skin 0.05 protein_coding missense_variant MODERATE 2919C>A Asn973Lys
M0029327 IFGCFLCK_00009 12362 11 Skin 0.11 protein_coding missense_variant MODERATE 3070A>G Thr1024Ala
M0029328 IFGCFLCK_00009 12367 11 Skin 0.11 protein_coding synonymous_variant LOW 3075C>G Gly1025Gly
M0029329 IFGCFLCK_00009 12372 11 Skin 0.11 protein_coding missense_variant MODERATE 3080A>C Tyr1027Ser
M0029330 IFGCFLCK_00009 12406 9 Skin 0.09 protein_coding synonymous_variant LOW 3114A>G Leu1038Leu
M0029331 IFGCFLCK_00009 12409 9 Skin 0.09 protein_coding synonymous_variant LOW 3117C>G Gly1039Gly
M0029332 IFGCFLCK_00009 12502 3 Skin 0.03 protein_coding synonymous_variant LOW 3210C>T Phe1070Phe
M0029333 IFGCFLCK_00010 17303 7 Skin 0.07 protein_coding missense_variant MODERATE 851A>G Asn284Ser
M0029334 IFGCFLCK_00002 632 18 Skin 0.19 protein_coding upstream_gene_variant MODIFIER -59C>T None
M0029335 IFGCFLCK_00002 664 18 Skin 0.19 protein_coding upstream_gene_variant MODIFIER -27G>T None
M0029336 IFGCFLCK_00002 665 18 Skin 0.19 protein_coding upstream_gene_variant MODIFIER -26C>T None
M0029337 IFGCFLCK_00004 1735 28 Skin 0.29 protein_coding synonymous_variant LOW 99C>T Phe33Phe
M0029338 IFGCFLCK_00005 2595 29 Skin 0.30 protein_coding synonymous_variant LOW 468T>C Gly156Gly
M0029339 IFGCFLCK_00005 2604 29 Skin 0.30 protein_coding synonymous_variant LOW 477G>C Leu159Leu
M0029340 IFGCFLCK_00005 2610 29 Skin 0.30 protein_coding synonymous_variant LOW 483G>A Glu161Glu
M0029341 IFGCFLCK_00005 2613 29 Skin 0.30 protein_coding synonymous_variant LOW 486T>C Arg162Arg
M0029342 IFGCFLCK_00005 2622 29 Skin 0.30 protein_coding synonymous_variant LOW 495A>G Glu165Glu
M0029343 IFGCFLCK_00005 2623 29 Skin 0.30 protein_coding missense_variant MODERATE 496A>C Lys166Gln
M0029344 IFGCFLCK_00005 2763 7 Skin 0.07 protein_coding synonymous_variant LOW 636T>C Asp212Asp
M0029345 IFGCFLCK_00005 2767 7 Skin 0.07 protein_coding missense_variant MODERATE 640C>G Gln214Glu
M0029346 IFGCFLCK_00005 3201 29 Skin 0.30 protein_coding synonymous_variant LOW 1074C>T Gly358Gly
M0029347 IFGCFLCK_00005 3229 29 Skin 0.30 protein_coding missense_variant MODERATE 1102C>A Gln368Lys
M0029348 IFGCFLCK_00005 3234 29 Skin 0.30 protein_coding synonymous_variant LOW 1107T>C Pro369Pro
M0029349 IFGCFLCK_00005 3426 30 Skin 0.31 protein_coding synonymous_variant LOW 1299T>C His433His
M0029350 IFGCFLCK_00005 3433 30 Skin 0.31 protein_coding missense_variant MODERATE 1306G>A Ala436Thr
M0029351 IFGCFLCK_00005 3439 30 Skin 0.31 protein_coding missense_variant MODERATE 1312G>A Val438Ile
M0029352 IFGCFLCK_00004 1648 6 Skin 0.06 protein_coding synonymous_variant LOW 12A>G Pro4Pro
M0029353 IFGCFLCK_00005 3321 5 Skin 0.05 protein_coding synonymous_variant LOW 1194T>C Gly398Gly
M0029354 IFGCFLCK_00005 3327 5 Skin 0.05 protein_coding synonymous_variant LOW 1200G>C Arg400Arg
M0029355 IFGCFLCK_00005 3358 6 Skin 0.06 protein_coding missense_variant MODERATE 1231A>C Lys411Gln
M0029356 IFGCFLCK_00005 4157 6 Skin 0.06 protein_coding missense_variant MODERATE 2030A>T Tyr677Phe
M0029357 IFGCFLCK_00005 4160 6 Skin 0.06 protein_coding missense_variant MODERATE 2033C>G Ser678Trp
M0029358 IFGCFLCK_00006 5428 3 Skin 0.03 protein_coding synonymous_variant LOW 1185A>G Lys395Lys
M0029359 IFGCFLCK_00006 5443 3 Skin 0.03 protein_coding synonymous_variant LOW 1200A>T Gly400Gly
M0029360 IFGCFLCK_00006 5446 3 Skin 0.03 protein_coding synonymous_variant LOW 1203G>C Gly401Gly
M0029361 IFGCFLCK_00007 5636 24 Skin 0.25 protein_coding upstream_gene_variant MODIFIER -214T>C None
M0029362 IFGCFLCK_00007 5724 14 Skin 0.15 protein_coding upstream_gene_variant MODIFIER -126C>G None
M0029363 IFGCFLCK_00009 11087 4 Skin 0.04 protein_coding missense_variant MODERATE 1795C>T Pro599Ser
M0029364 IFGCFLCK_00004 1924 3 Skin 0.03 protein_coding synonymous_variant LOW 288A>G Pro96Pro
M0029365 IFGCFLCK_00006 4906 3 Skin 0.03 protein_coding synonymous_variant LOW 663C>T Asp221Asp
M0029366 IFGCFLCK_00006 4907 3 Skin 0.03 protein_coding synonymous_variant LOW 664T>C Leu222Leu
M0029367 IFGCFLCK_00006 4927 3 Skin 0.03 protein_coding synonymous_variant LOW 684G>A Ala228Ala
M0029368 IFGCFLCK_00006 5369 3 Skin 0.03 protein_coding synonymous_variant LOW 1126C>T Leu376Leu
M0029369 IFGCFLCK_00009 13075 3 Skin 0.03 protein_coding synonymous_variant LOW 3783T>C Pro1261Pro
M0029370 IFGCFLCK_00009 13108 3 Skin 0.03 protein_coding synonymous_variant LOW 3816C>T Asp1272Asp
M0029371 IFGCFLCK_00009 13111 3 Skin 0.03 protein_coding synonymous_variant LOW 3819T>C Asn1273Asn
M0029372 IFGCFLCK_00009 13115 3 Skin 0.03 protein_coding synonymous_variant LOW 3823C>T Leu1275Leu
M0029373 IFGCFLCK_00009 13120 3 Skin 0.03 protein_coding synonymous_variant LOW 3828A>G Leu1276Leu
M0029374 IFGCFLCK_00009 13126 3 Skin 0.03 protein_coding synonymous_variant LOW 3834A>T Thr1278Thr
M0029375 IFGCFLCK_00009 15893 5 Skin 0.05 protein_coding missense_variant MODERATE 6601A>G Asn2201Asp
M0029376 IFGCFLCK_00007 5640 10 Skin 0.10 protein_coding upstream_gene_variant MODIFIER -210C>G None
M0029377 IFGCFLCK_00007 5641 10 Skin 0.10 protein_coding upstream_gene_variant MODIFIER -209G>C None
M0029378 IFGCFLCK_00007 5664 11 Skin 0.11 protein_coding upstream_gene_variant MODIFIER -186G>A None
M0029379 IFGCFLCK_00007 7082 11 Skin 0.11 protein_coding synonymous_variant LOW 1233T>C Arg411Arg
M0029380 IFGCFLCK_00003 1305 5 Skin 0.05 protein_coding synonymous_variant LOW 180T>C Arg60Arg
M0029381 IFGCFLCK_00008 8017 6 Skin 0.06 protein_coding synonymous_variant LOW 759C>T Ser253Ser
M0029382 IFGCFLCK_00009 11618 3 Skin 0.03 protein_coding missense_variant MODERATE 2326T>A Ser776Thr
M0029383 IFGCFLCK_00007 5778 3 Skin 0.03 protein_coding upstream_gene_variant MODIFIER -72C>T None
M0029384 IFGCFLCK_00009 14305 3 Skin 0.03 protein_coding synonymous_variant LOW 5013C>G Leu1671Leu
M0029385 IFGCFLCK_00009 14320 3 Skin 0.03 protein_coding synonymous_variant LOW 5028G>T Leu1676Leu
M0029386 IFGCFLCK_00008 8917 6 Skin 0.06 protein_coding upstream_gene_variant MODIFIER -142C>T None
M0029387 IFGCFLCK_00009 12982 5 Skin 0.05 protein_coding synonymous_variant LOW 3690A>G Ala1230Ala
M0029388 IFGCFLCK_00009 13012 4 Skin 0.04 protein_coding synonymous_variant LOW 3720T>C Cys1240Cys
M0029389 IFGCFLCK_00009 11905 7 Skin 0.07 protein_coding synonymous_variant LOW 2613C>A Gly871Gly
M0029390 IFGCFLCK_00011 18018 6 Skin 0.06 protein_coding synonymous_variant LOW 204G>A Leu68Leu
M0029391 IFGCFLCK_00011 18048 6 Skin 0.06 protein_coding synonymous_variant LOW 234G>A Thr78Thr
M0029392 IFGCFLCK_00011 18822 4 Skin 0.04 protein_coding synonymous_variant LOW 1008G>A Glu336Glu
M0029393 IFGCFLCK_00011 19177 5 Skin 0.05 protein_coding synonymous_variant LOW 1363T>C Leu455Leu
M0029394 IFGCFLCK_00009 9854 3 Skin 0.03 protein_coding stop_gained HIGH 562G>T Glu188*
M0029395 IFGCFLCK_00009 9855 3 Skin 0.03 protein_coding missense_variant MODERATE 563A>C Glu188Ala
M0029396 IFGCFLCK_00009 9863 3 Skin 0.03 protein_coding missense_variant MODERATE 571A>G Asn191Asp
M0029397 IFGCFLCK_00007 6005 3 Skin 0.03 protein_coding synonymous_variant LOW 156T>C Ile52Ile
M0029398 IFGCFLCK_00009 9500 6 Skin 0.06 protein_coding missense_variant MODERATE 208A>C Ile70Leu
M0029399 IFGCFLCK_00009 12610 3 Skin 0.03 protein_coding synonymous_variant LOW 3318T>G Val1106Val
M0029400 IFGCFLCK_00009 12631 3 Skin 0.03 protein_coding synonymous_variant LOW 3339G>A Gly1113Gly
M0029401 IFGCFLCK_00009 11513 6 Skin 0.06 protein_coding missense_variant MODERATE 2221A>G Ile741Val
M0029402 IFGCFLCK_00009 11530 6 Skin 0.06 protein_coding synonymous_variant LOW 2238C>T Arg746Arg
M0029403 IFGCFLCK_00009 9688 8 Skin 0.08 protein_coding synonymous_variant LOW 396C>G Ser132Ser
M0029404 IFGCFLCK_00009 12012 3 Skin 0.03 protein_coding missense_variant MODERATE 2720G>A Ser907Asn
M0029405 IFGCFLCK_00009 12034 3 Skin 0.03 protein_coding synonymous_variant LOW 2742A>G Glu914Glu
M0029406 IFGCFLCK_00009 12037 3 Skin 0.03 protein_coding synonymous_variant LOW 2745C>T Leu915Leu
M0029407 IFGCFLCK_00009 12039 3 Skin 0.03 protein_coding missense_variant MODERATE 2747G>A Ser916Asn
M0029408 IFGCFLCK_00009 12043 3 Skin 0.03 protein_coding synonymous_variant LOW 2751G>A Glu917Glu
M0029409 IFGCFLCK_00009 12716 4 Skin 0.04 protein_coding missense_variant MODERATE 3424A>G Ser1142Gly
M0029410 IFGCFLCK_00009 11380 3 Skin 0.03 protein_coding synonymous_variant LOW 2088A>C Arg696Arg
M0029411 IFGCFLCK_00009 9409 3 Skin 0.03 protein_coding missense_variant MODERATE 117C>A His39Gln
M0029412 IFGCFLCK_00009 12817 3 Skin 0.03 protein_coding missense_variant MODERATE 3525T>A Asp1175Glu
M0029413 IFGCFLCK_00009 12829 3 Skin 0.03 protein_coding missense_variant MODERATE 3537C>A His1179Gln
M0029414 IFGCFLCK_00009 12856 3 Skin 0.03 protein_coding synonymous_variant LOW 3564T>G Ala1188Ala
M0029415 IFGCFLCK_00009 12862 3 Skin 0.03 protein_coding synonymous_variant LOW 3570C>T Arg1190Arg
M0029416 IFGCFLCK_00009 12886 3 Skin 0.03 protein_coding synonymous_variant LOW 3594A>T Arg1198Arg
M0029417 IFGCFLCK_00009 12908 3 Skin 0.03 protein_coding missense_variant MODERATE 3616C>G His1206Asp
M0029418 IFGCFLCK_00009 12913 3 Skin 0.03 protein_coding missense_variant MODERATE 3621C>A Asn1207Lys






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant
IFGCFLCK_00005 PHI:10663 oprC 73.4 1.4e-303 14 685 0.9810 0.9516 rodents nosocomial infection outer membrane copper receptor reduced virulence






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
IFGCFLCK_00007 AWK41767.1|GH13_3 75.1 7.14e-240 11 459 0.9473 0.9594





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
IFGCFLCK_00005 1.B.14.4.1 73.4 3e-303 14 685 0.9810 0.9516 1 Channels/Pores 1.B β-Barrel Porins 1.B.14 The Outer Membrane Receptor (OMR) Family
IFGCFLCK_00007 2.A.3.1.7 75.4 2.6e-192 9 459 0.9515 0.9596 2 Electrochemical Potential-driven Transporters 2.A Porters (uniporters, symporters, antiporters) 2.A.3 The Amino Acid-Polyamine-Organocation (APC) Family