Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C2196
  Reference Plasmid   1111525849747079_bin.18__k141_98632
  Reference Plasmid Size   6103
  Reference Plasmid GC Content   0.67
  Reference Plasmid Mobility Type   non-mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0029614 NLFGKENC_00002 780 11 Skin 0.69 protein_coding synonymous_variant LOW 204A>G Val68Val
M0029615 NLFGKENC_00002 781 11 Skin 0.69 protein_coding synonymous_variant LOW 205A>C Arg69Arg
M0029616 NLFGKENC_00002 789 11 Skin 0.69 protein_coding synonymous_variant LOW 213T>C Thr71Thr
M0029617 NLFGKENC_00002 828 9 Skin 0.56 protein_coding synonymous_variant LOW 252C>T Gly84Gly
M0029618 NLFGKENC_00002 876 11 Skin 0.69 protein_coding synonymous_variant LOW 300C>G Pro100Pro
M0029619 NLFGKENC_00002 930 13 Skin 0.81 protein_coding synonymous_variant LOW 354G>C Arg118Arg
M0029620 NLFGKENC_00002 984 12 Skin 0.75 protein_coding synonymous_variant LOW 408C>G Leu136Leu
M0029621 NLFGKENC_00002 1056 9 Skin 0.56 protein_coding synonymous_variant LOW 480A>G Ala160Ala
M0029622 NLFGKENC_00002 1095 8 Skin 0.50 protein_coding synonymous_variant LOW 519G>C Pro173Pro
M0029623 NLFGKENC_00002 1129 9 Skin 0.56 protein_coding synonymous_variant LOW 553T>C Leu185Leu
M0029624 NLFGKENC_00002 1164 9 Skin 0.56 protein_coding synonymous_variant LOW 588C>T Arg196Arg
M0029625 NLFGKENC_00004 2239 10 Skin 0.63 protein_coding synonymous_variant LOW 42T>C Asp14Asp
M0029626 NLFGKENC_00005 2914 9 Skin 0.56 protein_coding synonymous_variant LOW 123G>A Glu41Glu
M0029627 NLFGKENC_00005 2944 8 Skin 0.50 protein_coding synonymous_variant LOW 153C>A Gly51Gly
M0029628 NLFGKENC_00005 3289 7 Skin 0.44 protein_coding synonymous_variant LOW 498C>A Ser166Ser
M0029629 NLFGKENC_00005 3292 7 Skin 0.44 protein_coding synonymous_variant LOW 501G>A Glu167Glu
M0029630 NLFGKENC_00005 3320 7 Skin 0.44 protein_coding missense_variant MODERATE 529A>G Ile177Val
M0029631 NLFGKENC_00001 110 3 Skin 0.19 protein_coding synonymous_variant LOW 99T>C Thr33Thr
M0029632 NLFGKENC_00001 111 3 Skin 0.19 protein_coding missense_variant MODERATE 100G>A Gly34Ser
M0029633 NLFGKENC_00002 621 4 Skin 0.25 protein_coding synonymous_variant LOW 45C>T Ser15Ser
M0029634 NLFGKENC_00003 1669 6 Skin 0.38 protein_coding synonymous_variant LOW 345G>A Thr115Thr
M0029635 NLFGKENC_00003 1678 8 Skin 0.50 protein_coding synonymous_variant LOW 354C>G Pro118Pro
M0029636 NLFGKENC_00003 1717 7 Skin 0.44 protein_coding synonymous_variant LOW 393A>G Val131Val
M0029637 NLFGKENC_00003 1765 8 Skin 0.50 protein_coding synonymous_variant LOW 441G>A Val147Val
M0029638 NLFGKENC_00005 2998 10 Skin 0.63 protein_coding synonymous_variant LOW 207C>T Asp69Asp
M0029639 NLFGKENC_00005 3007 10 Skin 0.63 protein_coding synonymous_variant LOW 216C>T Ala72Ala
M0029640 NLFGKENC_00007 4471 4 Skin 0.25 protein_coding synonymous_variant LOW 447A>C Leu149Leu
M0029641 NLFGKENC_00007 4510 5 Skin 0.31 protein_coding synonymous_variant LOW 408G>A Ala136Ala
M0029642 NLFGKENC_00007 4549 6 Skin 0.38 protein_coding synonymous_variant LOW 369T>C Ala123Ala
M0029643 NLFGKENC_00001 176 3 Skin 0.19 protein_coding synonymous_variant LOW 165G>A Glu55Glu
M0029644 NLFGKENC_00001 200 3 Skin 0.19 protein_coding synonymous_variant LOW 189T>C Ile63Ile
M0029645 NLFGKENC_00001 224 3 Skin 0.19 protein_coding synonymous_variant LOW 213A>G Glu71Glu
M0029646 NLFGKENC_00001 245 3 Skin 0.19 protein_coding synonymous_variant LOW 234G>A Val78Val
M0029647 NLFGKENC_00001 266 4 Skin 0.25 protein_coding synonymous_variant LOW 255A>G Gln85Gln
M0029648 NLFGKENC_00001 350 3 Skin 0.19 protein_coding synonymous_variant LOW 339C>G Ala113Ala
M0029649 NLFGKENC_00001 353 3 Skin 0.19 protein_coding synonymous_variant LOW 342C>G Ala114Ala
M0029650 NLFGKENC_00001 362 3 Skin 0.19 protein_coding synonymous_variant LOW 351G>A Lys117Lys
M0029651 NLFGKENC_00001 365 3 Skin 0.19 protein_coding synonymous_variant LOW 354A>G Lys118Lys
M0029652 NLFGKENC_00001 367 3 Skin 0.19 protein_coding missense_variant MODERATE 356T>A Met119Lys
M0029653 NLFGKENC_00001 371 3 Skin 0.19 protein_coding synonymous_variant LOW 360C>T Val120Val
M0029654 NLFGKENC_00001 377 3 Skin 0.19 protein_coding synonymous_variant LOW 366G>A Glu122Glu
M0029655 NLFGKENC_00001 383 3 Skin 0.19 protein_coding synonymous_variant LOW 372T>C Asp124Asp
M0029656 NLFGKENC_00001 384 3 Skin 0.19 protein_coding missense_variant MODERATE 373G>C Glu125Gln
M0029657 NLFGKENC_00003 1344 5 Skin 0.31 protein_coding missense_variant MODERATE 20T>C Phe7Ser
M0029658 NLFGKENC_00003 1870 6 Skin 0.38 protein_coding synonymous_variant LOW 546A>G Ala182Ala
M0029659 NLFGKENC_00003 1966 6 Skin 0.38 protein_coding synonymous_variant LOW 642C>T Gly214Gly
M0029660 NLFGKENC_00006 3941 6 Skin 0.38 protein_coding synonymous_variant LOW 402C>T Arg134Arg
M0029661 NLFGKENC_00006 3961 6 Skin 0.38 protein_coding missense_variant MODERATE 422T>C Val141Ala
M0029662 NLFGKENC_00007 4210 5 Skin 0.31 protein_coding synonymous_variant LOW 708T>C Arg236Arg
M0029663 NLFGKENC_00007 4221 5 Skin 0.31 protein_coding synonymous_variant LOW 697T>C Leu233Leu
M0029664 NLFGKENC_00008 5660 7 Skin 0.44 protein_coding missense_variant MODERATE 307G>A Asp103Asn
M0029665 NLFGKENC_00008 5687 7 Skin 0.44 protein_coding missense_variant MODERATE 280G>C Gly94Arg
M0029666 NLFGKENC_00008 5705 7 Skin 0.44 protein_coding missense_variant MODERATE 262C>T Leu88Phe
M0029667 NLFGKENC_00008 5708 7 Skin 0.44 protein_coding missense_variant MODERATE 259C>G Gln87Glu
M0029668 NLFGKENC_00008 5815 5 Skin 0.31 protein_coding missense_variant MODERATE 152C>G Ala51Gly
M0029669 NLFGKENC_00007 5980 8 Skin 0.50 protein_coding upstream_gene_variant MODIFIER -1063C>A None
M0029670 NLFGKENC_00007 5987 3 Skin 0.19 protein_coding upstream_gene_variant MODIFIER -1070C>T None
M0029671 NLFGKENC_00007 6002 3 Skin 0.19 protein_coding upstream_gene_variant MODIFIER -1085C>G None
M0029672 NLFGKENC_00007 6044 4 Skin 0.25 protein_coding upstream_gene_variant MODIFIER -1127C>T None
M0029673 NLFGKENC_00003 1837 5 Skin 0.31 protein_coding synonymous_variant LOW 513G>A Glu171Glu
M0029674 NLFGKENC_00003 2072 6 Skin 0.38 protein_coding synonymous_variant LOW 748T>C Leu250Leu
M0029675 NLFGKENC_00006 3872 5 Skin 0.31 protein_coding synonymous_variant LOW 333C>T Arg111Arg
M0029676 NLFGKENC_00007 4660 4 Skin 0.25 protein_coding synonymous_variant LOW 258G>T Val86Val
M0029677 NLFGKENC_00007 4669 4 Skin 0.25 protein_coding synonymous_variant LOW 249T>C His83His
M0029678 NLFGKENC_00007 4690 4 Skin 0.25 protein_coding synonymous_variant LOW 228C>G Leu76Leu
M0029679 NLFGKENC_00003 1477 3 Skin 0.19 protein_coding synonymous_variant LOW 153A>G Leu51Leu
M0029680 NLFGKENC_00003 1482 3 Skin 0.19 protein_coding missense_variant MODERATE 158A>C Lys53Thr
M0029681 NLFGKENC_00003 1486 3 Skin 0.19 protein_coding synonymous_variant LOW 162G>A Leu54Leu
M0029682 NLFGKENC_00003 1489 3 Skin 0.19 protein_coding synonymous_variant LOW 165A>G Pro55Pro
M0029683 NLFGKENC_00003 1556 4 Skin 0.25 protein_coding missense_variant MODERATE 232A>G Thr78Ala
M0029684 NLFGKENC_00004 2464 8 Skin 0.50 protein_coding synonymous_variant LOW 267T>G Ala89Ala
M0029685 NLFGKENC_00004 2476 8 Skin 0.50 protein_coding synonymous_variant LOW 279T>C Tyr93Tyr
M0029686 NLFGKENC_00004 2515 9 Skin 0.56 protein_coding synonymous_variant LOW 318A>G Glu106Glu
M0029687 NLFGKENC_00004 2528 9 Skin 0.56 protein_coding missense_variant MODERATE 331T>G Tyr111Asp
M0029688 NLFGKENC_00007 4591 3 Skin 0.19 protein_coding synonymous_variant LOW 327C>T Ile109Ile
M0029689 NLFGKENC_00007 4594 3 Skin 0.19 protein_coding synonymous_variant LOW 324T>C Cys108Cys
M0029690 NLFGKENC_00007 4597 3 Skin 0.19 protein_coding synonymous_variant LOW 321C>G Leu107Leu
M0029691 NLFGKENC_00007 4603 3 Skin 0.19 protein_coding synonymous_variant LOW 315A>G Pro105Pro
M0029692 NLFGKENC_00007 4615 3 Skin 0.19 protein_coding synonymous_variant LOW 303G>C Ser101Ser
M0029693 NLFGKENC_00007 4762 4 Skin 0.25 protein_coding synonymous_variant LOW 156A>G Val52Val
M0029694 NLFGKENC_00007 4777 4 Skin 0.25 protein_coding synonymous_variant LOW 141A>G Ala47Ala
M0029695 NLFGKENC_00007 4798 4 Skin 0.25 protein_coding synonymous_variant LOW 120C>G Leu40Leu
M0029696 NLFGKENC_00007 4081 3 Skin 0.19 protein_coding synonymous_variant LOW 837C>T Ser279Ser






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term