Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C16
  Reference Plasmid   1111525849752591_bin.18__k141_378613
  Reference Plasmid Size   2697
  Reference Plasmid GC Content   0.37
  Reference Plasmid Mobility Type   non-mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0030227 EKMEEMFE_00002 1062 3 Skin 0.60 protein_coding synonymous_variant LOW 357C>T Asp119Asp
M0030228 EKMEEMFE_00002 1149 3 Skin 0.60 protein_coding synonymous_variant LOW 270T>C Asn90Asn
M0030229 EKMEEMFE_00002 1283 3 Skin 0.60 protein_coding missense_variant MODERATE 136A>G Thr46Ala
M0030230 EKMEEMFE_00002 1383 3 Skin 0.60 protein_coding synonymous_variant LOW 36C>A Ser12Ser
M0030231 EKMEEMFE_00003 1493 3 Skin 0.60 protein_coding missense_variant MODERATE 787T>C Phe263Leu
M0030232 EKMEEMFE_00003 1570 3 Skin 0.60 protein_coding missense_variant MODERATE 710C>A Ala237Glu
M0030233 EKMEEMFE_00003 1890 3 Skin 0.60 protein_coding synonymous_variant LOW 390G>A Glu130Glu
M0030234 EKMEEMFE_00003 1973 3 Skin 0.60 protein_coding missense_variant MODERATE 307A>G Met103Val
M0030235 EKMEEMFE_00003 2151 3 Skin 0.60 protein_coding synonymous_variant LOW 129C>T Asp43Asp
M0030236 EKMEEMFE_00003 2154 3 Skin 0.60 protein_coding synonymous_variant LOW 126A>G Thr42Thr
M0030237 EKMEEMFE_00003 2265 3 Skin 0.60 protein_coding synonymous_variant LOW 15A>G Leu5Leu
M0030238 EKMEEMFE_00004 2333 3 Skin 0.60 protein_coding synonymous_variant LOW 300C>T Arg100Arg
M0030239 EKMEEMFE_00004 2396 3 Skin 0.60 protein_coding synonymous_variant LOW 237G>A Thr79Thr
M0030240 EKMEEMFE_00004 2399 3 Skin 0.60 protein_coding synonymous_variant LOW 234A>G Arg78Arg
M0030241 EKMEEMFE_00004 2425 3 Skin 0.60 protein_coding missense_variant MODERATE 208A>G Lys70Glu
M0030242 EKMEEMFE_00004 2434 3 Skin 0.60 protein_coding stop_gained HIGH 199A>T Lys67*
M0030243 EKMEEMFE_00004 2437 3 Skin 0.60 protein_coding missense_variant MODERATE 196G>A Glu66Lys
M0030244 EKMEEMFE_00004 2442 3 Skin 0.60 protein_coding missense_variant MODERATE 191T>C Val64Ala
M0030245 EKMEEMFE_00004 2443 3 Skin 0.60 protein_coding missense_variant MODERATE 190G>A Val64Ile
M0030246 EKMEEMFE_00004 2454 3 Skin 0.60 protein_coding missense_variant MODERATE 179G>A Arg60Gln
M0030247 EKMEEMFE_00004 2462 3 Skin 0.60 protein_coding synonymous_variant LOW 171A>G Lys57Lys
M0030248 EKMEEMFE_00004 2474 3 Skin 0.60 protein_coding synonymous_variant LOW 159C>G Thr53Thr
M0030249 EKMEEMFE_00004 2475 3 Skin 0.60 protein_coding missense_variant MODERATE 158C>A Thr53Asn
M0030250 EKMEEMFE_00004 2477 3 Skin 0.60 protein_coding missense_variant MODERATE 156A>G Ile52Met
M0030251 EKMEEMFE_00004 2501 3 Skin 0.60 protein_coding synonymous_variant LOW 132C>T Ile44Ile
M0030252 EKMEEMFE_00004 2558 3 Skin 0.60 protein_coding synonymous_variant LOW 75C>G Gly25Gly
M0030253 EKMEEMFE_00001 521 4 Skin 0.80 protein_coding downstream_gene_variant MODIFIER *13T>G None
M0030254 EKMEEMFE_00001 594 4 Skin 0.80 protein_coding synonymous_variant LOW 360T>C His120His
M0030255 EKMEEMFE_00001 725 4 Skin 0.80 protein_coding missense_variant MODERATE 229A>G Met77Val
M0030256 EKMEEMFE_00001 848 4 Skin 0.80 protein_coding missense_variant MODERATE 106A>G Ile36Val
M0030257 EKMEEMFE_00001 858 4 Skin 0.80 protein_coding synonymous_variant LOW 96A>T Ile32Ile
M0030258 EKMEEMFE_00001 879 4 Skin 0.80 protein_coding synonymous_variant LOW 75G>A Leu25Leu
M0118420 EKMEEMFE_00001 915 3 Oral 1.00 protein_coding synonymous_variant LOW 39C>T Arg13Arg
M0118421 EKMEEMFE_00002 1259 3 Oral 1.00 protein_coding missense_variant MODERATE 160T>C Tyr54His
M0118422 EKMEEMFE_00003 1570 3 Oral 1.00 protein_coding missense_variant MODERATE 710C>A Ala237Glu
M0118423 EKMEEMFE_00003 1611 3 Oral 1.00 protein_coding synonymous_variant LOW 669T>C Thr223Thr
M0118424 EKMEEMFE_00003 1643 3 Oral 1.00 protein_coding synonymous_variant LOW 637A>C Arg213Arg
M0118425 EKMEEMFE_00003 1647 3 Oral 1.00 protein_coding synonymous_variant LOW 633C>T Leu211Leu
M0118426 EKMEEMFE_00003 1890 3 Oral 1.00 protein_coding synonymous_variant LOW 390G>A Glu130Glu
M0118427 EKMEEMFE_00003 1935 3 Oral 1.00 protein_coding synonymous_variant LOW 345C>T Val115Val
M0118428 EKMEEMFE_00003 2121 3 Oral 1.00 protein_coding synonymous_variant LOW 159T>C Asn53Asn
M0118429 EKMEEMFE_00003 2142 3 Oral 1.00 protein_coding synonymous_variant LOW 138T>C Ser46Ser
M0118430 EKMEEMFE_00003 2154 3 Oral 1.00 protein_coding synonymous_variant LOW 126A>G Thr42Thr
M0118431 EKMEEMFE_00003 2265 3 Oral 1.00 protein_coding synonymous_variant LOW 15A>G Leu5Leu
M0118432 EKMEEMFE_00004 2333 3 Oral 1.00 protein_coding synonymous_variant LOW 300C>T Arg100Arg
M0118433 EKMEEMFE_00004 2363 3 Oral 1.00 protein_coding synonymous_variant LOW 270T>A Val90Val






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term