Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C2218
  Reference Plasmid   1111525849753200_bin.14__k141_129638
  Reference Plasmid Size   4028
  Reference Plasmid GC Content   0.44
  Reference Plasmid Mobility Type   non-mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0030300 CILEMHJN_00002 1456 7 Skin 0.29 protein_coding synonymous_variant LOW 288C>T Arg96Arg
M0030301 CILEMHJN_00002 1579 14 Skin 0.58 protein_coding synonymous_variant LOW 411G>A Lys137Lys
M0030302 CILEMHJN_00002 1679 14 Skin 0.58 protein_coding missense_variant MODERATE 511A>G Lys171Glu
M0030303 CILEMHJN_00002 1711 3 Skin 0.13 protein_coding synonymous_variant LOW 543T>C Phe181Phe
M0030304 CILEMHJN_00002 1771 13 Skin 0.54 protein_coding synonymous_variant LOW 603G>A Glu201Glu
M0030305 CILEMHJN_00002 1772 13 Skin 0.54 protein_coding synonymous_variant LOW 604A>C Arg202Arg
M0030306 CILEMHJN_00002 1801 13 Skin 0.54 protein_coding synonymous_variant LOW 633C>T Val211Val
M0030307 CILEMHJN_00003 1965 17 Skin 0.71 protein_coding missense_variant MODERATE 23T>C Val8Ala
M0030308 CILEMHJN_00003 1967 17 Skin 0.71 protein_coding missense_variant MODERATE 25A>G Asn9Asp
M0030309 CILEMHJN_00003 2008 17 Skin 0.71 protein_coding synonymous_variant LOW 66A>G Ala22Ala
M0030310 CILEMHJN_00003 2152 22 Skin 0.92 protein_coding synonymous_variant LOW 210A>C Val70Val
M0030311 CILEMHJN_00003 2155 22 Skin 0.92 protein_coding synonymous_variant LOW 213A>G Val71Val
M0030312 CILEMHJN_00003 2158 22 Skin 0.92 protein_coding synonymous_variant LOW 216A>G Leu72Leu
M0030313 CILEMHJN_00003 2161 21 Skin 0.88 protein_coding synonymous_variant LOW 219G>A Leu73Leu
M0030314 CILEMHJN_00003 2260 4 Skin 0.17 protein_coding synonymous_variant LOW 318G>A Val106Val
M0030315 CILEMHJN_00003 2306 5 Skin 0.21 protein_coding missense_variant MODERATE 364A>C Asn122His
M0030316 CILEMHJN_00003 2311 5 Skin 0.21 protein_coding synonymous_variant LOW 369C>T Ser123Ser
M0030317 CILEMHJN_00003 2458 17 Skin 0.71 protein_coding synonymous_variant LOW 516G>C Ala172Ala
M0030318 CILEMHJN_00003 2561 6 Skin 0.25 protein_coding missense_variant MODERATE 619G>A Val207Met
M0030319 CILEMHJN_00003 2569 10 Skin 0.42 protein_coding synonymous_variant LOW 627T>C Arg209Arg
M0030320 CILEMHJN_00003 2741 17 Skin 0.71 protein_coding missense_variant MODERATE 799C>G Gln267Glu
M0030321 CILEMHJN_00003 3091 14 Skin 0.58 protein_coding synonymous_variant LOW 1149A>G Leu383Leu
M0030322 CILEMHJN_00003 3098 14 Skin 0.58 protein_coding missense_variant MODERATE 1156T>A Phe386Ile
M0030323 CILEMHJN_00003 3161 17 Skin 0.71 protein_coding missense_variant MODERATE 1219C>G Gln407Glu
M0030324 CILEMHJN_00003 3586 3 Skin 0.13 protein_coding synonymous_variant LOW 1644C>A Ala548Ala
M0030325 CILEMHJN_00003 3664 3 Skin 0.13 protein_coding synonymous_variant LOW 1722A>C Ala574Ala
M0030326 CILEMHJN_00001 3884 6 Skin 0.25 protein_coding downstream_gene_variant MODIFIER *3011A>G None
M0030327 CILEMHJN_00002 1300 11 Skin 0.46 protein_coding synonymous_variant LOW 132A>G Lys44Lys
M0030328 CILEMHJN_00002 1309 11 Skin 0.46 protein_coding synonymous_variant LOW 141A>C Leu47Leu
M0030329 CILEMHJN_00002 1312 10 Skin 0.42 protein_coding synonymous_variant LOW 144G>C Ser48Ser
M0030330 CILEMHJN_00002 1348 4 Skin 0.17 protein_coding synonymous_variant LOW 180T>C Asn60Asn
M0030331 CILEMHJN_00002 1369 5 Skin 0.21 protein_coding synonymous_variant LOW 201G>C Leu67Leu
M0030332 CILEMHJN_00002 1384 5 Skin 0.21 protein_coding synonymous_variant LOW 216C>T Asp72Asp
M0030333 CILEMHJN_00002 1447 4 Skin 0.17 protein_coding synonymous_variant LOW 279G>A Leu93Leu
M0030334 CILEMHJN_00002 1651 13 Skin 0.54 protein_coding missense_variant MODERATE 483A>G Ile161Met
M0030335 CILEMHJN_00002 1654 10 Skin 0.42 protein_coding synonymous_variant LOW 486A>G Ala162Ala
M0030336 CILEMHJN_00002 1660 4 Skin 0.17 protein_coding synonymous_variant LOW 492A>G Glu164Glu
M0030337 CILEMHJN_00002 1714 3 Skin 0.13 protein_coding synonymous_variant LOW 546T>C Ile182Ile
M0030338 CILEMHJN_00002 1723 11 Skin 0.46 protein_coding synonymous_variant LOW 555T>G Arg185Arg
M0030339 CILEMHJN_00002 1837 11 Skin 0.46 protein_coding synonymous_variant LOW 669T>C Ser223Ser
M0030340 CILEMHJN_00002 1882 13 Skin 0.54 protein_coding synonymous_variant LOW 714C>G Ala238Ala
M0030341 CILEMHJN_00003 2149 8 Skin 0.33 protein_coding synonymous_variant LOW 207G>C Gly69Gly
M0030342 CILEMHJN_00003 2176 17 Skin 0.71 protein_coding synonymous_variant LOW 234C>T Gly78Gly
M0030343 CILEMHJN_00003 2209 4 Skin 0.17 protein_coding synonymous_variant LOW 267T>G Leu89Leu
M0030344 CILEMHJN_00003 2210 12 Skin 0.50 protein_coding synonymous_variant LOW 268A>C Arg90Arg
M0030345 CILEMHJN_00003 2254 9 Skin 0.38 protein_coding synonymous_variant LOW 312G>C Thr104Thr
M0030346 CILEMHJN_00003 2257 9 Skin 0.38 protein_coding synonymous_variant LOW 315C>T Gly105Gly
M0030347 CILEMHJN_00003 2528 13 Skin 0.54 protein_coding missense_variant MODERATE 586G>A Val196Ile
M0030348 CILEMHJN_00003 2534 18 Skin 0.75 protein_coding missense_variant MODERATE 592A>G Thr198Ala
M0030349 CILEMHJN_00003 2540 12 Skin 0.50 protein_coding missense_variant MODERATE 598T>A Leu200Met
M0030350 CILEMHJN_00003 2590 15 Skin 0.63 protein_coding synonymous_variant LOW 648G>C Ala216Ala
M0030351 CILEMHJN_00003 2597 15 Skin 0.63 protein_coding missense_variant MODERATE 655G>A Asp219Asn
M0030352 CILEMHJN_00003 2623 13 Skin 0.54 protein_coding synonymous_variant LOW 681A>G Thr227Thr
M0030353 CILEMHJN_00003 2636 13 Skin 0.54 protein_coding missense_variant MODERATE 694A>G Ile232Val
M0030354 CILEMHJN_00003 2642 15 Skin 0.63 protein_coding missense_variant MODERATE 700A>G Ser234Gly
M0030355 CILEMHJN_00003 2704 14 Skin 0.58 protein_coding synonymous_variant LOW 762T>G Gly254Gly
M0030356 CILEMHJN_00003 2746 5 Skin 0.21 protein_coding synonymous_variant LOW 804G>A Ala268Ala
M0030357 CILEMHJN_00003 2758 16 Skin 0.67 protein_coding synonymous_variant LOW 816C>A Pro272Pro
M0030358 CILEMHJN_00003 2768 14 Skin 0.58 protein_coding synonymous_variant LOW 826C>T Leu276Leu
M0030359 CILEMHJN_00003 2800 3 Skin 0.13 protein_coding synonymous_variant LOW 858T>C Gly286Gly
M0030360 CILEMHJN_00003 2808 15 Skin 0.63 protein_coding missense_variant MODERATE 866T>C Ile289Thr
M0030361 CILEMHJN_00003 2839 4 Skin 0.17 protein_coding missense_variant MODERATE 897A>T Leu299Phe
M0030362 CILEMHJN_00003 2851 4 Skin 0.17 protein_coding synonymous_variant LOW 909C>T Gly303Gly
M0030363 CILEMHJN_00003 2859 14 Skin 0.58 protein_coding missense_variant MODERATE 917G>T Gly306Val
M0030364 CILEMHJN_00003 2929 16 Skin 0.67 protein_coding synonymous_variant LOW 987T>C Ser329Ser
M0030365 CILEMHJN_00003 2962 14 Skin 0.58 protein_coding synonymous_variant LOW 1020T>C Gly340Gly
M0030366 CILEMHJN_00003 2992 13 Skin 0.54 protein_coding synonymous_variant LOW 1050A>G Val350Val
M0030367 CILEMHJN_00003 2995 13 Skin 0.54 protein_coding synonymous_variant LOW 1053A>G Ala351Ala
M0030368 CILEMHJN_00003 3034 10 Skin 0.42 protein_coding synonymous_variant LOW 1092T>C Ile364Ile
M0030369 CILEMHJN_00003 3070 4 Skin 0.17 protein_coding synonymous_variant LOW 1128G>A Thr376Thr
M0030370 CILEMHJN_00003 3073 11 Skin 0.46 protein_coding synonymous_variant LOW 1131T>A Pro377Pro
M0030371 CILEMHJN_00003 3110 10 Skin 0.42 protein_coding missense_variant MODERATE 1168C>A Leu390Ile
M0030372 CILEMHJN_00003 3121 11 Skin 0.46 protein_coding missense_variant MODERATE 1179C>A Asn393Lys
M0030373 CILEMHJN_00003 3126 10 Skin 0.42 protein_coding missense_variant MODERATE 1184C>A Thr395Lys
M0030374 CILEMHJN_00003 3130 10 Skin 0.42 protein_coding synonymous_variant LOW 1188T>C Pro396Pro
M0030375 CILEMHJN_00003 3169 11 Skin 0.46 protein_coding synonymous_variant LOW 1227G>A Ala409Ala
M0030376 CILEMHJN_00003 3238 12 Skin 0.50 protein_coding synonymous_variant LOW 1296G>A Glu432Glu
M0030377 CILEMHJN_00003 3244 12 Skin 0.50 protein_coding synonymous_variant LOW 1302T>C Thr434Thr
M0030378 CILEMHJN_00002 1855 3 Skin 0.13 protein_coding synonymous_variant LOW 687A>G Lys229Lys
M0030379 CILEMHJN_00003 2092 3 Skin 0.13 protein_coding synonymous_variant LOW 150A>G Pro50Pro
M0030380 CILEMHJN_00003 2404 13 Skin 0.54 protein_coding synonymous_variant LOW 462A>G Gln154Gln
M0030381 CILEMHJN_00002 1084 5 Skin 0.21 protein_coding upstream_gene_variant MODIFIER -85G>A None
M0030382 CILEMHJN_00002 1104 5 Skin 0.21 protein_coding upstream_gene_variant MODIFIER -65A>T None
M0030383 CILEMHJN_00002 1111 7 Skin 0.29 protein_coding upstream_gene_variant MODIFIER -58C>T None
M0030384 CILEMHJN_00002 1118 5 Skin 0.21 protein_coding upstream_gene_variant MODIFIER -51T>G None
M0030385 CILEMHJN_00002 1134 5 Skin 0.21 protein_coding upstream_gene_variant MODIFIER -35C>T None
M0030386 CILEMHJN_00002 1155 6 Skin 0.25 protein_coding upstream_gene_variant MODIFIER -14T>G None
M0030387 CILEMHJN_00002 1156 6 Skin 0.25 protein_coding upstream_gene_variant MODIFIER -13C>G None
M0030388 CILEMHJN_00002 1157 6 Skin 0.25 protein_coding upstream_gene_variant MODIFIER -12G>A None
M0030389 CILEMHJN_00002 1159 6 Skin 0.25 protein_coding upstream_gene_variant MODIFIER -10T>G None
M0030390 CILEMHJN_00002 1247 10 Skin 0.42 protein_coding missense_variant MODERATE 79A>G Ser27Gly
M0030391 CILEMHJN_00002 1270 3 Skin 0.13 protein_coding synonymous_variant LOW 102T>C Val34Val
M0030392 CILEMHJN_00002 1676 3 Skin 0.13 protein_coding missense_variant MODERATE 508G>A Gly170Ser
M0030393 CILEMHJN_00002 1810 4 Skin 0.17 protein_coding synonymous_variant LOW 642A>G Thr214Thr
M0030394 CILEMHJN_00003 2584 5 Skin 0.21 protein_coding synonymous_variant LOW 642C>T Phe214Phe
M0030395 CILEMHJN_00003 2587 3 Skin 0.13 protein_coding synonymous_variant LOW 645G>A Val215Val
M0030396 CILEMHJN_00003 2797 12 Skin 0.50 protein_coding synonymous_variant LOW 855T>C Val285Val
M0030397 CILEMHJN_00003 3184 11 Skin 0.46 protein_coding synonymous_variant LOW 1242A>C Gly414Gly
M0030398 CILEMHJN_00002 1324 3 Skin 0.13 protein_coding synonymous_variant LOW 156C>T Ala52Ala
M0030399 CILEMHJN_00002 1597 4 Skin 0.17 protein_coding synonymous_variant LOW 429C>G Gly143Gly
M0030400 CILEMHJN_00002 1600 5 Skin 0.21 protein_coding synonymous_variant LOW 432G>A Ser144Ser
M0030401 CILEMHJN_00003 2767 7 Skin 0.29 protein_coding synonymous_variant LOW 825G>T Gly275Gly
M0030402 CILEMHJN_00002 1555 9 Skin 0.38 protein_coding synonymous_variant LOW 387G>A Leu129Leu
M0030403 CILEMHJN_00002 1696 6 Skin 0.25 protein_coding synonymous_variant LOW 528G>A Val176Val
M0030404 CILEMHJN_00002 1746 11 Skin 0.46 protein_coding missense_variant MODERATE 578C>G Pro193Arg
M0030405 CILEMHJN_00002 1909 8 Skin 0.33 protein_coding synonymous_variant LOW 741T>A Ile247Ile
M0030406 CILEMHJN_00002 1915 8 Skin 0.33 protein_coding synonymous_variant LOW 747C>T Val249Val
M0030407 CILEMHJN_00003 2111 7 Skin 0.29 protein_coding missense_variant MODERATE 169G>A Gly57Ser
M0030408 CILEMHJN_00003 2113 7 Skin 0.29 protein_coding synonymous_variant LOW 171T>C Gly57Gly
M0030409 CILEMHJN_00003 2159 7 Skin 0.29 protein_coding synonymous_variant LOW 217C>T Leu73Leu
M0030410 CILEMHJN_00003 2278 8 Skin 0.33 protein_coding synonymous_variant LOW 336G>T Ser112Ser
M0030411 CILEMHJN_00003 2299 9 Skin 0.38 protein_coding synonymous_variant LOW 357G>T Arg119Arg
M0030412 CILEMHJN_00003 3307 3 Skin 0.13 protein_coding synonymous_variant LOW 1365C>T Tyr455Tyr
M0030413 CILEMHJN_00003 2806 7 Skin 0.29 protein_coding synonymous_variant LOW 864C>T Leu288Leu
M0030414 CILEMHJN_00002 1477 4 Skin 0.17 protein_coding synonymous_variant LOW 309G>A Ser103Ser
M0030415 CILEMHJN_00002 1033 3 Skin 0.13 protein_coding upstream_gene_variant MODIFIER -136T>C None
M0030416 CILEMHJN_00002 1035 3 Skin 0.13 protein_coding upstream_gene_variant MODIFIER -134T>C None
M0030417 CILEMHJN_00002 1045 3 Skin 0.13 protein_coding upstream_gene_variant MODIFIER -124A>G None
M0030418 CILEMHJN_00002 1051 3 Skin 0.13 protein_coding upstream_gene_variant MODIFIER -118C>G None
M0030419 CILEMHJN_00002 1204 3 Skin 0.13 protein_coding synonymous_variant LOW 36C>T Asn12Asn
M0030420 CILEMHJN_00003 2581 6 Skin 0.25 protein_coding synonymous_variant LOW 639G>A Lys213Lys
M0030421 CILEMHJN_00003 2740 3 Skin 0.13 protein_coding synonymous_variant LOW 798A>G Leu266Leu
M0030422 CILEMHJN_00002 1465 5 Skin 0.21 protein_coding synonymous_variant LOW 297G>C Gly99Gly
M0030423 CILEMHJN_00002 1471 3 Skin 0.13 protein_coding synonymous_variant LOW 303T>C Tyr101Tyr
M0030424 CILEMHJN_00003 2032 4 Skin 0.17 protein_coding synonymous_variant LOW 90A>G Ala30Ala
M0030425 CILEMHJN_00003 3417 3 Skin 0.13 protein_coding missense_variant MODERATE 1475T>C Val492Ala
M0030426 CILEMHJN_00003 2029 3 Skin 0.13 protein_coding synonymous_variant LOW 87T>C Leu29Leu






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term