Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C2230
  Reference Plasmid   1111525849753914_bin.29__k141_428227
  Reference Plasmid Size   22466
  Reference Plasmid GC Content   0.69
  Reference Plasmid Mobility Type   non-mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0030509 KFCFIDNB_00019 20050 3 Skin 0.23 protein_coding synonymous_variant LOW 642T>C Arg214Arg
M0030510 KFCFIDNB_00005 99 3 Skin 0.23 protein_coding upstream_gene_variant MODIFIER -4466G>T None
M0030511 KFCFIDNB_00003 1251 3 Skin 0.23 protein_coding synonymous_variant LOW 1890A>C Ala630Ala
M0030512 KFCFIDNB_00003 1252 3 Skin 0.23 protein_coding missense_variant MODERATE 1889C>T Ala630Val
M0030513 KFCFIDNB_00005 4903 3 Skin 0.23 protein_coding synonymous_variant LOW 339T>C Gly113Gly
M0030514 KFCFIDNB_00005 5041 3 Skin 0.23 protein_coding synonymous_variant LOW 477A>G Arg159Arg
M0030515 KFCFIDNB_00005 5440 3 Skin 0.23 protein_coding synonymous_variant LOW 876T>C Ala292Ala
M0030516 KFCFIDNB_00008 7296 3 Skin 0.23 protein_coding synonymous_variant LOW 493T>C Leu165Leu
M0030517 KFCFIDNB_00008 7544 3 Skin 0.23 protein_coding missense_variant MODERATE 741T>G Ile247Met
M0030518 KFCFIDNB_00008 7802 3 Skin 0.23 protein_coding synonymous_variant LOW 999T>C Ala333Ala
M0030519 KFCFIDNB_00017 18022 3 Skin 0.23 protein_coding synonymous_variant LOW 405A>C Ala135Ala
M0030520 KFCFIDNB_00017 18170 3 Skin 0.23 protein_coding missense_variant MODERATE 257G>A Gly86Asp
M0030521 KFCFIDNB_00018 19256 3 Skin 0.23 protein_coding synonymous_variant LOW 675T>G Leu225Leu
M0030522 KFCFIDNB_00019 19652 3 Skin 0.23 protein_coding missense_variant MODERATE 244A>G Thr82Ala
M0030523 KFCFIDNB_00019 19755 3 Skin 0.23 protein_coding missense_variant MODERATE 347A>G Asp116Gly
M0030524 KFCFIDNB_00011 15746 3 Skin 0.23 protein_coding upstream_gene_variant MODIFIER -4706A>G None
M0030525 KFCFIDNB_00016 16560 3 Skin 0.23 protein_coding missense_variant MODERATE 428A>G Gln143Arg
M0030526 KFCFIDNB_00017 16919 3 Skin 0.23 protein_coding missense_variant MODERATE 1508A>G Lys503Arg
M0030527 KFCFIDNB_00010 8889 3 Skin 0.23 protein_coding synonymous_variant LOW 1071T>C Thr357Thr
M0030528 KFCFIDNB_00010 9766 3 Skin 0.23 protein_coding missense_variant MODERATE 194C>G Ala65Gly
M0030529 KFCFIDNB_00011 10924 3 Skin 0.23 protein_coding synonymous_variant LOW 117T>C Val39Val
M0030530 KFCFIDNB_00012 11790 3 Skin 0.23 protein_coding synonymous_variant LOW 345A>C Ala115Ala
M0030531 KFCFIDNB_00012 11806 3 Skin 0.23 protein_coding missense_variant MODERATE 329G>A Gly110Asp
M0030532 KFCFIDNB_00012 11808 3 Skin 0.23 protein_coding missense_variant MODERATE 327C>G His109Gln
M0030533 KFCFIDNB_00012 11809 3 Skin 0.23 protein_coding missense_variant MODERATE 326A>G His109Arg
M0030534 KFCFIDNB_00012 11811 3 Skin 0.23 protein_coding synonymous_variant LOW 324T>C Cys108Cys
M0030535 KFCFIDNB_00012 11820 3 Skin 0.23 protein_coding synonymous_variant LOW 315C>G Val105Val
M0030536 KFCFIDNB_00012 11823 3 Skin 0.23 protein_coding synonymous_variant LOW 312C>G Ala104Ala
M0030537 KFCFIDNB_00013 12771 3 Skin 0.23 protein_coding missense_variant MODERATE 266A>G His89Arg






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
KFCFIDNB_00010 AYF03582.1|GH103 100 2.32000000161579e-315 1 427 1 1
KFCFIDNB_00012 AYF03584.1|CE9 100 1.19e-256 1 365 1 1





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
KFCFIDNB_00008 2.A.23.1.8 70.5 3.5e-170 3 459 0.9892 0.9870 2 Electrochemical Potential-driven Transporters 2.A Porters (uniporters, symporters, antiporters) 2.A.23 The Dicarboxylate/Amino Acid:Cation (Na+ or H+) Symporter (DAACS) Family