Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C2256
  Reference Plasmid   1111525849758379_bin.23__k141_63081
  Reference Plasmid Size   4780
  Reference Plasmid GC Content   0.44
  Reference Plasmid Mobility Type   non-mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0031930 NMCBKONN_00001 761 3 Skin 0.60 protein_coding synonymous_variant LOW 1419A>G Gly473Gly
M0031931 NMCBKONN_00001 818 3 Skin 0.60 protein_coding synonymous_variant LOW 1362T>C Val454Val
M0031932 NMCBKONN_00001 881 3 Skin 0.60 protein_coding synonymous_variant LOW 1299T>C Ile433Ile
M0031933 NMCBKONN_00001 996 3 Skin 0.60 protein_coding missense_variant MODERATE 1184A>G Asn395Ser
M0031934 NMCBKONN_00001 1034 3 Skin 0.60 protein_coding synonymous_variant LOW 1146T>C Thr382Thr
M0031935 NMCBKONN_00001 1087 3 Skin 0.60 protein_coding synonymous_variant LOW 1093T>C Leu365Leu
M0031936 NMCBKONN_00001 1100 3 Skin 0.60 protein_coding synonymous_variant LOW 1080A>C Val360Val
M0031937 NMCBKONN_00001 1244 3 Skin 0.60 protein_coding synonymous_variant LOW 936G>A Ala312Ala
M0031938 NMCBKONN_00001 1322 3 Skin 0.60 protein_coding synonymous_variant LOW 858A>G Val286Val
M0031939 NMCBKONN_00001 1676 3 Skin 0.60 protein_coding synonymous_variant LOW 504G>A Arg168Arg
M0031940 NMCBKONN_00002 2434 3 Skin 0.60 protein_coding synonymous_variant LOW 360A>G Thr120Thr
M0031941 NMCBKONN_00002 2452 3 Skin 0.60 protein_coding missense_variant MODERATE 342G>T Glu114Asp
M0031942 NMCBKONN_00002 2453 3 Skin 0.60 protein_coding missense_variant MODERATE 341A>G Glu114Gly
M0031943 NMCBKONN_00002 2671 4 Skin 0.80 protein_coding synonymous_variant LOW 123G>A Gln41Gln
M0031944 NMCBKONN_00001 2820 3 Skin 0.60 protein_coding upstream_gene_variant MODIFIER -641T>C None
M0031945 NMCBKONN_00003 2996 4 Skin 0.80 protein_coding synonymous_variant LOW 807A>T Gly269Gly
M0031946 NMCBKONN_00003 3110 3 Skin 0.60 protein_coding synonymous_variant LOW 693G>A Gly231Gly
M0031947 NMCBKONN_00003 3137 3 Skin 0.60 protein_coding synonymous_variant LOW 666C>T Gly222Gly
M0031948 NMCBKONN_00003 3167 4 Skin 0.80 protein_coding synonymous_variant LOW 636C>G Pro212Pro
M0031949 NMCBKONN_00003 3359 3 Skin 0.60 protein_coding synonymous_variant LOW 444C>T Ser148Ser
M0031950 NMCBKONN_00003 3401 4 Skin 0.80 protein_coding synonymous_variant LOW 402G>A Ala134Ala
M0031951 NMCBKONN_00003 3404 4 Skin 0.80 protein_coding synonymous_variant LOW 399T>C Gly133Gly
M0031952 NMCBKONN_00003 3467 4 Skin 0.80 protein_coding synonymous_variant LOW 336C>T Arg112Arg
M0031953 NMCBKONN_00003 3473 3 Skin 0.60 protein_coding synonymous_variant LOW 330C>T Val110Val
M0031954 NMCBKONN_00003 3596 4 Skin 0.80 protein_coding synonymous_variant LOW 207T>C Asn69Asn
M0031955 NMCBKONN_00003 3605 3 Skin 0.60 protein_coding synonymous_variant LOW 198G>A Gln66Gln
M0031956 NMCBKONN_00003 3734 4 Skin 0.80 protein_coding synonymous_variant LOW 69C>A Gly23Gly
M0031957 NMCBKONN_00001 3855 3 Skin 0.60 protein_coding upstream_gene_variant MODIFIER -1676C>G None
M0031958 NMCBKONN_00004 4316 3 Skin 0.60 protein_coding synonymous_variant LOW 339T>C Phe113Phe
M0031959 NMCBKONN_00002 2644 3 Skin 0.60 protein_coding synonymous_variant LOW 150T>C Asn50Asn
M0031960 NMCBKONN_00003 3542 3 Skin 0.60 protein_coding synonymous_variant LOW 261G>A Leu87Leu
M0031961 NMCBKONN_00003 3545 3 Skin 0.60 protein_coding synonymous_variant LOW 258C>T Asp86Asp






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term