Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C2266
  Reference Plasmid   1111525849758568_bin.24__k141_657894
  Reference Plasmid Size   45219
  Reference Plasmid GC Content   0.50
  Reference Plasmid Mobility Type   non-mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0032046 DOGLHIAF_00006 2848 3 Skin 0.07 protein_coding synonymous_variant LOW 696A>G Pro232Pro
M0032047 DOGLHIAF_00023 18972 17 Skin 0.40 protein_coding synonymous_variant LOW 1218C>A Ala406Ala
M0032048 DOGLHIAF_00023 18977 7 Skin 0.17 protein_coding missense_variant MODERATE 1223G>T Arg408Leu
M0032049 DOGLHIAF_00023 18978 6 Skin 0.14 protein_coding synonymous_variant LOW 1224C>T Arg408Arg
M0032050 DOGLHIAF_00023 18981 7 Skin 0.17 protein_coding synonymous_variant LOW 1227A>G Ser409Ser
M0032051 DOGLHIAF_00042 41914 5 Skin 0.12 protein_coding upstream_gene_variant MODIFIER -3815A>G None
M0032052 DOGLHIAF_00050 43067 3 Skin 0.07 protein_coding missense_variant MODERATE 184G>A Gly62Ser
M0032053 DOGLHIAF_00050 43081 3 Skin 0.07 protein_coding synonymous_variant LOW 198A>G Val66Val
M0032054 DOGLHIAF_00050 43222 7 Skin 0.17 protein_coding synonymous_variant LOW 339T>C Arg113Arg
M0032055 DOGLHIAF_00046 43239 7 Skin 0.17 protein_coding upstream_gene_variant MODIFIER -4019C>T None
M0032056 DOGLHIAF_00051 43242 7 Skin 0.17 protein_coding splice_region_variant&stop_retained_variant LOW 431A>G Ter144Ter
M0032057 DOGLHIAF_00047 44238 11 Skin 0.26 protein_coding upstream_gene_variant MODIFIER -3127A>G None
M0032058 DOGLHIAF_00047 45146 15 Skin 0.36 protein_coding upstream_gene_variant MODIFIER -4035C>G None
M0032059 DOGLHIAF_00047 45150 15 Skin 0.36 protein_coding upstream_gene_variant MODIFIER -4039C>G None
M0032060 DOGLHIAF_00047 45158 15 Skin 0.36 protein_coding upstream_gene_variant MODIFIER -4047A>G None
M0032061 DOGLHIAF_00051 43313 3 Skin 0.07 protein_coding synonymous_variant LOW 360T>C Ala120Ala
M0032062 DOGLHIAF_00046 43877 8 Skin 0.19 protein_coding upstream_gene_variant MODIFIER -4657A>T None
M0032063 DOGLHIAF_00046 43896 7 Skin 0.17 protein_coding upstream_gene_variant MODIFIER -4676G>A None
M0032064 DOGLHIAF_00052 43940 8 Skin 0.19 protein_coding missense_variant MODERATE 272A>G Asp91Gly
M0032065 DOGLHIAF_00052 43987 9 Skin 0.21 protein_coding synonymous_variant LOW 225G>C Gly75Gly
M0032066 DOGLHIAF_00046 43700 4 Skin 0.10 protein_coding upstream_gene_variant MODIFIER -4480A>G None
M0032067 DOGLHIAF_00047 45065 4 Skin 0.10 protein_coding upstream_gene_variant MODIFIER -3954G>T None
M0032068 DOGLHIAF_00014 11784 3 Skin 0.07 protein_coding missense_variant MODERATE 74G>A Gly25Asp
M0032069 DOGLHIAF_00038 38633 3 Skin 0.07 protein_coding upstream_gene_variant MODIFIER -3909C>A None
M0032070 DOGLHIAF_00038 38663 3 Skin 0.07 protein_coding upstream_gene_variant MODIFIER -3939C>G None
M0032071 DOGLHIAF_00046 38696 3 Skin 0.07 protein_coding synonymous_variant LOW 525T>C Ala175Ala






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant
DOGLHIAF_00012 PHI:5564 opgG 79.1 1.4e-255 1 522 1.0000 1.0000 eudicots soft rot glucans biosynthesis protein reduced virulence
DOGLHIAF_00048 PHI:560 sirA 88.5 5e-103 1 216 0.9908 0.9954 rodents gastroenteritis None reduced virulence






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
DOGLHIAF_00011 SMZ56255.1|GT2 98.5 0 1 853 1 1
DOGLHIAF_00028 SMZ56241.1|GH4 97.9 1.73e-304 1 433 1 1





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
DOGLHIAF_00011 4.D.3.1.1 74.7 0 1 847 0.9930 0.9894 4 Group Translocators 4.D Polysaccharide Synthase/Exporters 4.D.3 The Glycan Glucosyl Transferase (OpgH) Family
DOGLHIAF_00016 2.A.36.6.11 76.1 2.2e-246 1 573 1.0000 0.9931 2 Electrochemical Potential-driven Transporters 2.A Porters (uniporters, symporters, antiporters) 2.A.36 The Monovalent Cation:Proton Antiporter-1 (CPA1) Family
DOGLHIAF_00025 2.A.1.6.4 79.3 5e-231 3 500 0.9960 0.9960 2 Electrochemical Potential-driven Transporters 2.A Porters (uniporters, symporters, antiporters) 2.A.1 The Major Facilitator Superfamily (MFS)
DOGLHIAF_00030 4.A.3.2.9 71 1.2e-30 1 100 0.9901 0.9524 4 Group Translocators 4.A Phosphotransfer-driven Group Translocators (PTS) 4.A.3 The PTS Lactose-N,N'-Diacetylchitobiose-β-glucoside (Lac) Family