Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C2374
  Reference Plasmid   1111525849768046_bin.37__k141_127168
  Reference Plasmid Size   9187
  Reference Plasmid GC Content   0.38
  Reference Plasmid Mobility Type   non-mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0119824 ANKNANPK_00006 4942 4 Oral 0.44 protein_coding synonymous_variant LOW 571T>C Leu191Leu
M0119825 ANKNANPK_00006 4982 3 Oral 0.33 protein_coding synonymous_variant LOW 531A>C Gly177Gly
M0119826 ANKNANPK_00006 5006 3 Oral 0.33 protein_coding synonymous_variant LOW 507A>G Glu169Glu
M0119827 ANKNANPK_00006 5099 3 Oral 0.33 protein_coding synonymous_variant LOW 414C>T Asp138Asp
M0119828 ANKNANPK_00006 5104 5 Oral 0.56 protein_coding missense_variant MODERATE 409A>C Ile137Leu
M0119829 ANKNANPK_00006 5122 5 Oral 0.56 protein_coding missense_variant MODERATE 391G>T Ala131Ser
M0119830 ANKNANPK_00006 5213 4 Oral 0.44 protein_coding synonymous_variant LOW 300T>C Ser100Ser
M0119831 ANKNANPK_00006 5228 3 Oral 0.33 protein_coding synonymous_variant LOW 285C>T Ile95Ile
M0119832 ANKNANPK_00006 5234 5 Oral 0.56 protein_coding synonymous_variant LOW 279C>T Leu93Leu
M0119833 ANKNANPK_00006 5246 4 Oral 0.44 protein_coding synonymous_variant LOW 267A>G Lys89Lys
M0119834 ANKNANPK_00006 5303 3 Oral 0.33 protein_coding synonymous_variant LOW 210C>T Thr70Thr
M0119835 ANKNANPK_00006 5345 4 Oral 0.44 protein_coding synonymous_variant LOW 168T>A Ala56Ala
M0119836 ANKNANPK_00007 5902 4 Oral 0.44 protein_coding synonymous_variant LOW 1134C>T His378His
M0119837 ANKNANPK_00007 5947 5 Oral 0.56 protein_coding missense_variant MODERATE 1089A>T Arg363Ser
M0119838 ANKNANPK_00007 5949 5 Oral 0.56 protein_coding synonymous_variant LOW 1087A>C Arg363Arg
M0119839 ANKNANPK_00007 5965 3 Oral 0.33 protein_coding synonymous_variant LOW 1071T>C Asn357Asn
M0119840 ANKNANPK_00007 6001 5 Oral 0.56 protein_coding synonymous_variant LOW 1035A>G Ala345Ala
M0119841 ANKNANPK_00007 6025 4 Oral 0.44 protein_coding synonymous_variant LOW 1011G>A Ala337Ala
M0119842 ANKNANPK_00007 6148 6 Oral 0.67 protein_coding synonymous_variant LOW 888G>A Val296Val
M0119843 ANKNANPK_00007 6151 6 Oral 0.67 protein_coding synonymous_variant LOW 885A>T Ala295Ala
M0119844 ANKNANPK_00007 6191 6 Oral 0.67 protein_coding missense_variant MODERATE 845T>C Val282Ala
M0119845 ANKNANPK_00007 6202 5 Oral 0.56 protein_coding synonymous_variant LOW 834C>T Gly278Gly
M0119846 ANKNANPK_00007 6231 6 Oral 0.67 protein_coding missense_variant MODERATE 805T>G Ser269Ala
M0119847 ANKNANPK_00007 6268 4 Oral 0.44 protein_coding synonymous_variant LOW 768A>T Ala256Ala
M0119848 ANKNANPK_00007 6286 3 Oral 0.33 protein_coding synonymous_variant LOW 750A>T Gly250Gly
M0119849 ANKNANPK_00007 6442 4 Oral 0.44 protein_coding synonymous_variant LOW 594C>T Asp198Asp
M0119850 ANKNANPK_00007 6483 6 Oral 0.67 protein_coding synonymous_variant LOW 553T>C Leu185Leu
M0119851 ANKNANPK_00007 6490 6 Oral 0.67 protein_coding synonymous_variant LOW 546T>C Asp182Asp
M0119852 ANKNANPK_00007 6598 6 Oral 0.67 protein_coding synonymous_variant LOW 438A>G Val146Val
M0119853 ANKNANPK_00007 6652 6 Oral 0.67 protein_coding synonymous_variant LOW 384G>T Gly128Gly
M0119854 ANKNANPK_00007 6691 4 Oral 0.44 protein_coding synonymous_variant LOW 345C>T Asp115Asp
M0119855 ANKNANPK_00007 6784 6 Oral 0.67 protein_coding synonymous_variant LOW 252T>C His84His
M0119856 ANKNANPK_00007 6790 6 Oral 0.67 protein_coding synonymous_variant LOW 246C>T Asn82Asn
M0119857 ANKNANPK_00007 6820 4 Oral 0.44 protein_coding synonymous_variant LOW 216A>G Glu72Glu
M0119858 ANKNANPK_00007 6844 6 Oral 0.67 protein_coding synonymous_variant LOW 192G>A Ala64Ala
M0119859 ANKNANPK_00007 6928 3 Oral 0.33 protein_coding synonymous_variant LOW 108G>A Lys36Lys
M0119860 ANKNANPK_00008 7309 4 Oral 0.44 protein_coding synonymous_variant LOW 252T>C Ile84Ile
M0119861 ANKNANPK_00008 7408 6 Oral 0.67 protein_coding synonymous_variant LOW 351T>C Asn117Asn
M0119862 ANKNANPK_00008 7492 3 Oral 0.33 protein_coding synonymous_variant LOW 435C>A Pro145Pro
M0119863 ANKNANPK_00008 7630 4 Oral 0.44 protein_coding synonymous_variant LOW 573T>C Asn191Asn
M0119864 ANKNANPK_00006 7680 3 Oral 0.33 protein_coding upstream_gene_variant MODIFIER -2168A>G None
M0119865 ANKNANPK_00006 7686 3 Oral 0.33 protein_coding upstream_gene_variant MODIFIER -2174A>G None
M0119866 ANKNANPK_00006 7713 3 Oral 0.33 protein_coding upstream_gene_variant MODIFIER -2201A>T None
M0119867 ANKNANPK_00006 7736 5 Oral 0.56 protein_coding upstream_gene_variant MODIFIER -2224A>C None
M0119868 ANKNANPK_00009 7939 4 Oral 0.44 protein_coding missense_variant MODERATE 113A>G Asn38Ser
M0119869 ANKNANPK_00009 8358 4 Oral 0.44 protein_coding missense_variant MODERATE 532G>A Val178Ile
M0119870 ANKNANPK_00009 8359 3 Oral 0.33 protein_coding missense_variant MODERATE 533T>C Val178Ala
M0119871 ANKNANPK_00006 8514 6 Oral 0.67 protein_coding upstream_gene_variant MODIFIER -3002A>T None
M0119872 ANKNANPK_00006 8633 5 Oral 0.56 protein_coding upstream_gene_variant MODIFIER -3121C>T None
M0119873 ANKNANPK_00006 8705 4 Oral 0.44 protein_coding upstream_gene_variant MODIFIER -3193A>T None
M0119874 ANKNANPK_00006 8720 5 Oral 0.56 protein_coding upstream_gene_variant MODIFIER -3208A>G None
M0119875 ANKNANPK_00006 8753 3 Oral 0.33 protein_coding upstream_gene_variant MODIFIER -3241A>G None
M0119876 ANKNANPK_00006 8786 5 Oral 0.56 protein_coding upstream_gene_variant MODIFIER -3274A>G None
M0119877 ANKNANPK_00006 8792 3 Oral 0.33 protein_coding upstream_gene_variant MODIFIER -3280C>T None
M0119878 ANKNANPK_00006 8837 5 Oral 0.56 protein_coding upstream_gene_variant MODIFIER -3325A>G None
M0119879 ANKNANPK_00006 8840 5 Oral 0.56 protein_coding upstream_gene_variant MODIFIER -3328T>C None
M0119880 ANKNANPK_00006 8960 5 Oral 0.56 protein_coding upstream_gene_variant MODIFIER -3448G>A None
M0119881 ANKNANPK_00006 9122 5 Oral 0.56 protein_coding upstream_gene_variant MODIFIER -3610T>C None
M0119882 ANKNANPK_00004 2152 3 Oral 0.33 protein_coding upstream_gene_variant MODIFIER -636G>T None
M0119883 ANKNANPK_00004 2186 4 Oral 0.44 protein_coding upstream_gene_variant MODIFIER -602C>T None
M0119884 ANKNANPK_00004 2195 4 Oral 0.44 protein_coding upstream_gene_variant MODIFIER -593G>T None
M0119885 ANKNANPK_00004 2199 4 Oral 0.44 protein_coding upstream_gene_variant MODIFIER -589G>A None
M0119886 ANKNANPK_00003 2419 3 Oral 0.33 protein_coding synonymous_variant LOW 126A>G Gly42Gly
M0119887 ANKNANPK_00003 2746 4 Oral 0.44 protein_coding upstream_gene_variant MODIFIER -202A>G None
M0119888 ANKNANPK_00003 2761 3 Oral 0.33 protein_coding upstream_gene_variant MODIFIER -217C>T None
M0119889 ANKNANPK_00004 2801 4 Oral 0.44 protein_coding missense_variant MODERATE 14T>C Val5Ala
M0119890 ANKNANPK_00004 2877 5 Oral 0.56 protein_coding synonymous_variant LOW 90A>G Val30Val
M0119891 ANKNANPK_00004 2904 5 Oral 0.56 protein_coding synonymous_variant LOW 117T>C Asn39Asn
M0119892 ANKNANPK_00004 3084 3 Oral 0.33 protein_coding synonymous_variant LOW 297G>A Leu99Leu
M0119893 ANKNANPK_00004 3117 3 Oral 0.33 protein_coding synonymous_variant LOW 330C>T Asp110Asp
M0119894 ANKNANPK_00004 3165 5 Oral 0.56 protein_coding synonymous_variant LOW 378T>C Asp126Asp
M0119895 ANKNANPK_00004 3250 5 Oral 0.56 protein_coding missense_variant MODERATE 463A>C Lys155Gln
M0119896 ANKNANPK_00004 3258 5 Oral 0.56 protein_coding synonymous_variant LOW 471A>G Lys157Lys
M0119897 ANKNANPK_00004 3267 5 Oral 0.56 protein_coding synonymous_variant LOW 480T>C Gly160Gly
M0119898 ANKNANPK_00004 3273 4 Oral 0.44 protein_coding synonymous_variant LOW 486T>C Tyr162Tyr
M0119899 ANKNANPK_00004 3291 4 Oral 0.44 protein_coding synonymous_variant LOW 504T>C Asp168Asp
M0119900 ANKNANPK_00004 3378 5 Oral 0.56 protein_coding synonymous_variant LOW 591T>C Gly197Gly
M0119901 ANKNANPK_00004 3390 5 Oral 0.56 protein_coding synonymous_variant LOW 603T>C Phe201Phe
M0119902 ANKNANPK_00004 3552 4 Oral 0.44 protein_coding synonymous_variant LOW 765C>T Cys255Cys
M0119903 ANKNANPK_00003 4019 3 Oral 0.33 protein_coding upstream_gene_variant MODIFIER -1475A>T None
M0119904 ANKNANPK_00003 4023 3 Oral 0.33 protein_coding upstream_gene_variant MODIFIER -1479A>G None
M0119905 ANKNANPK_00003 4031 3 Oral 0.33 protein_coding upstream_gene_variant MODIFIER -1487G>A None
M0119906 ANKNANPK_00003 4096 4 Oral 0.44 protein_coding upstream_gene_variant MODIFIER -1552T>C None
M0119907 ANKNANPK_00003 4098 4 Oral 0.44 protein_coding upstream_gene_variant MODIFIER -1554G>A None
M0119908 ANKNANPK_00003 4361 4 Oral 0.44 protein_coding upstream_gene_variant MODIFIER -1817C>T None
M0119909 ANKNANPK_00003 4399 4 Oral 0.44 protein_coding upstream_gene_variant MODIFIER -1855T>G None
M0119910 ANKNANPK_00003 4405 4 Oral 0.44 protein_coding upstream_gene_variant MODIFIER -1861A>G None
M0119911 ANKNANPK_00006 4475 4 Oral 0.44 protein_coding synonymous_variant LOW 1038A>T Pro346Pro
M0119912 ANKNANPK_00006 4490 4 Oral 0.44 protein_coding synonymous_variant LOW 1023T>C His341His
M0119913 ANKNANPK_00006 4501 4 Oral 0.44 protein_coding missense_variant MODERATE 1012A>G Thr338Ala
M0119914 ANKNANPK_00006 4694 3 Oral 0.33 protein_coding synonymous_variant LOW 819T>C Asp273Asp
M0119915 ANKNANPK_00006 4709 4 Oral 0.44 protein_coding synonymous_variant LOW 804G>C Thr268Thr
M0119916 ANKNANPK_00006 4720 3 Oral 0.33 protein_coding missense_variant MODERATE 793C>G Pro265Ala
M0119917 ANKNANPK_00006 4721 3 Oral 0.33 protein_coding synonymous_variant LOW 792A>T Pro264Pro
M0119918 ANKNANPK_00006 4772 4 Oral 0.44 protein_coding synonymous_variant LOW 741T>C Asp247Asp
M0119919 ANKNANPK_00006 4883 3 Oral 0.33 protein_coding synonymous_variant LOW 630A>G Lys210Lys
M0119920 ANKNANPK_00006 4898 3 Oral 0.33 protein_coding synonymous_variant LOW 615G>A Glu205Glu
M0119921 ANKNANPK_00006 4906 4 Oral 0.44 protein_coding synonymous_variant LOW 607C>T Leu203Leu
M0119922 ANKNANPK_00003 5515 5 Oral 0.56 protein_coding upstream_gene_variant MODIFIER -2971C>T None
M0119923 ANKNANPK_00007 5782 4 Oral 0.44 protein_coding synonymous_variant LOW 1254T>C Ser418Ser
M0119924 ANKNANPK_00007 6943 3 Oral 0.33 protein_coding synonymous_variant LOW 93A>T Ala31Ala
M0119925 ANKNANPK_00006 7778 3 Oral 0.33 protein_coding upstream_gene_variant MODIFIER -2266C>T None
M0119926 ANKNANPK_00003 2287 3 Oral 0.33 protein_coding synonymous_variant LOW 258T>C Ser86Ser
M0119927 ANKNANPK_00004 2841 3 Oral 0.33 protein_coding synonymous_variant LOW 54C>T Arg18Arg
M0119928 ANKNANPK_00004 3783 4 Oral 0.44 protein_coding synonymous_variant LOW 996T>C Asn332Asn
M0119929 ANKNANPK_00004 3804 3 Oral 0.33 protein_coding synonymous_variant LOW 1017T>C Ser339Ser
M0119930 ANKNANPK_00005 4287 3 Oral 0.33 protein_coding synonymous_variant LOW 138A>G Gly46Gly
M0119931 ANKNANPK_00005 4344 3 Oral 0.33 protein_coding synonymous_variant LOW 195G>A Lys65Lys
M0119932 ANKNANPK_00006 5498 4 Oral 0.44 protein_coding synonymous_variant LOW 15A>C Leu5Leu
M0119933 ANKNANPK_00007 6277 3 Oral 0.33 protein_coding synonymous_variant LOW 759A>G Leu253Leu
M0119934 ANKNANPK_00008 7462 3 Oral 0.33 protein_coding synonymous_variant LOW 405A>T Arg135Arg
M0119935 ANKNANPK_00004 3687 3 Oral 0.33 protein_coding synonymous_variant LOW 900A>T Ser300Ser
M0119936 ANKNANPK_00003 4090 3 Oral 0.33 protein_coding upstream_gene_variant MODIFIER -1546T>A None
M0119937 ANKNANPK_00003 4092 3 Oral 0.33 protein_coding upstream_gene_variant MODIFIER -1548C>T None
M0119938 ANKNANPK_00003 4094 3 Oral 0.33 protein_coding upstream_gene_variant MODIFIER -1550C>T None
M0119939 ANKNANPK_00003 4097 3 Oral 0.33 protein_coding upstream_gene_variant MODIFIER -1553A>C None
M0119940 ANKNANPK_00003 4103 3 Oral 0.33 protein_coding upstream_gene_variant MODIFIER -1559G>A None
M0119941 ANKNANPK_00003 4109 3 Oral 0.33 protein_coding upstream_gene_variant MODIFIER -1565A>C None
M0119942 ANKNANPK_00003 4110 3 Oral 0.33 protein_coding upstream_gene_variant MODIFIER -1566C>T None
M0119943 ANKNANPK_00006 4589 3 Oral 0.33 protein_coding synonymous_variant LOW 924G>A Ala308Ala






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term