Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C2422
  Reference Plasmid   1111525849768046_bin.37__k141_211790
  Reference Plasmid Size   8783
  Reference Plasmid GC Content   0.38
  Reference Plasmid Mobility Type   non-mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0124416 MLKENHAC_00002 1906 4 Oral 0.40 protein_coding synonymous_variant LOW 873A>T Leu291Leu
M0124417 MLKENHAC_00002 1916 4 Oral 0.40 protein_coding missense_variant MODERATE 863G>T Arg288Leu
M0124418 MLKENHAC_00002 1934 3 Oral 0.30 protein_coding missense_variant MODERATE 845T>C Ile282Thr
M0124419 MLKENHAC_00002 1954 4 Oral 0.40 protein_coding synonymous_variant LOW 825A>G Glu275Glu
M0124420 MLKENHAC_00002 1994 4 Oral 0.40 protein_coding missense_variant MODERATE 785G>A Arg262Lys
M0124421 MLKENHAC_00002 2053 3 Oral 0.30 protein_coding synonymous_variant LOW 726T>C Arg242Arg
M0124422 MLKENHAC_00002 2068 3 Oral 0.30 protein_coding synonymous_variant LOW 711A>T Pro237Pro
M0124423 MLKENHAC_00002 2071 3 Oral 0.30 protein_coding synonymous_variant LOW 708C>T Ser236Ser
M0124424 MLKENHAC_00002 2077 3 Oral 0.30 protein_coding synonymous_variant LOW 702C>T Ile234Ile
M0124425 MLKENHAC_00002 2121 3 Oral 0.30 protein_coding missense_variant MODERATE 658T>G Ser220Ala
M0124426 MLKENHAC_00002 2122 3 Oral 0.30 protein_coding synonymous_variant LOW 657A>G Lys219Lys
M0124427 MLKENHAC_00002 2162 4 Oral 0.40 protein_coding missense_variant MODERATE 617T>C Ile206Thr
M0124428 MLKENHAC_00002 2164 3 Oral 0.30 protein_coding synonymous_variant LOW 615C>T Asp205Asp
M0124429 MLKENHAC_00002 2169 3 Oral 0.30 protein_coding missense_variant MODERATE 610A>G Lys204Glu
M0124430 MLKENHAC_00002 2434 4 Oral 0.40 protein_coding synonymous_variant LOW 345A>C Ile115Ile
M0124431 MLKENHAC_00002 2482 3 Oral 0.30 protein_coding synonymous_variant LOW 297T>C Asp99Asp
M0124432 MLKENHAC_00002 2557 3 Oral 0.30 protein_coding missense_variant MODERATE 222T>G Asp74Glu
M0124433 MLKENHAC_00002 2656 3 Oral 0.30 protein_coding synonymous_variant LOW 123C>T Leu41Leu
M0124434 MLKENHAC_00002 2686 3 Oral 0.30 protein_coding synonymous_variant LOW 93A>T Pro31Pro
M0124435 MLKENHAC_00002 2805 3 Oral 0.30 protein_coding upstream_gene_variant MODIFIER -27T>C None
M0124436 MLKENHAC_00002 2842 3 Oral 0.30 protein_coding upstream_gene_variant MODIFIER -64C>A None
M0124437 MLKENHAC_00002 2849 3 Oral 0.30 protein_coding upstream_gene_variant MODIFIER -71G>A None
M0124438 MLKENHAC_00003 3216 4 Oral 0.40 protein_coding synonymous_variant LOW 717G>A Val239Val
M0124439 MLKENHAC_00003 3249 3 Oral 0.30 protein_coding synonymous_variant LOW 684C>T Asp228Asp
M0124440 MLKENHAC_00003 3261 4 Oral 0.40 protein_coding synonymous_variant LOW 672T>C Phe224Phe
M0124441 MLKENHAC_00003 3275 4 Oral 0.40 protein_coding missense_variant MODERATE 658T>G Tyr220Asp
M0124442 MLKENHAC_00003 3294 3 Oral 0.30 protein_coding synonymous_variant LOW 639G>A Val213Val
M0124443 MLKENHAC_00003 3303 4 Oral 0.40 protein_coding synonymous_variant LOW 630A>G Val210Val
M0124444 MLKENHAC_00003 3411 4 Oral 0.40 protein_coding synonymous_variant LOW 522T>C Ser174Ser
M0124445 MLKENHAC_00003 3465 3 Oral 0.30 protein_coding synonymous_variant LOW 468A>G Val156Val
M0124446 MLKENHAC_00003 3468 3 Oral 0.30 protein_coding synonymous_variant LOW 465C>T Ser155Ser
M0124447 MLKENHAC_00003 3498 3 Oral 0.30 protein_coding missense_variant MODERATE 435G>T Gln145His
M0124448 MLKENHAC_00003 3543 3 Oral 0.30 protein_coding synonymous_variant LOW 390T>A Ala130Ala
M0124449 MLKENHAC_00003 3554 3 Oral 0.30 protein_coding synonymous_variant LOW 379C>T Leu127Leu
M0124450 MLKENHAC_00003 3570 5 Oral 0.50 protein_coding synonymous_variant LOW 363T>C Cys121Cys
M0124451 MLKENHAC_00003 3587 5 Oral 0.50 protein_coding missense_variant MODERATE 346A>G Ile116Val
M0124452 MLKENHAC_00003 3592 5 Oral 0.50 protein_coding missense_variant MODERATE 341G>C Ser114Thr
M0124453 MLKENHAC_00003 3599 5 Oral 0.50 protein_coding missense_variant MODERATE 334C>A Gln112Lys
M0124454 MLKENHAC_00003 3603 4 Oral 0.40 protein_coding synonymous_variant LOW 330C>T Ile110Ile
M0124455 MLKENHAC_00003 3661 3 Oral 0.30 protein_coding missense_variant MODERATE 272T>C Met91Thr
M0124456 MLKENHAC_00003 3664 3 Oral 0.30 protein_coding missense_variant MODERATE 269G>A Arg90Gln
M0124457 MLKENHAC_00003 3741 4 Oral 0.40 protein_coding synonymous_variant LOW 192G>A Arg64Arg
M0124458 MLKENHAC_00003 3816 5 Oral 0.50 protein_coding synonymous_variant LOW 117A>G Ala39Ala
M0124459 MLKENHAC_00003 3833 3 Oral 0.30 protein_coding missense_variant MODERATE 100T>C Ser34Pro
M0124460 MLKENHAC_00003 3835 3 Oral 0.30 protein_coding missense_variant MODERATE 98T>A Ile33Lys
M0124461 MLKENHAC_00002 3961 3 Oral 0.30 protein_coding upstream_gene_variant MODIFIER -1183A>G None
M0124462 MLKENHAC_00004 4028 5 Oral 0.50 protein_coding synonymous_variant LOW 36T>C Asn12Asn
M0124463 MLKENHAC_00002 1876 3 Oral 0.30 protein_coding synonymous_variant LOW 903T>C Ala301Ala
M0124464 MLKENHAC_00002 2011 3 Oral 0.30 protein_coding synonymous_variant LOW 768G>A Gln256Gln
M0124465 MLKENHAC_00003 3794 4 Oral 0.40 protein_coding synonymous_variant LOW 139T>C Leu47Leu
M0124466 MLKENHAC_00003 3925 4 Oral 0.40 protein_coding missense_variant MODERATE 8C>A Pro3Gln
M0124467 MLKENHAC_00004 4196 3 Oral 0.30 protein_coding synonymous_variant LOW 204G>A Glu68Glu
M0124468 MLKENHAC_00004 4200 3 Oral 0.30 protein_coding missense_variant MODERATE 208A>G Ser70Gly
M0124469 MLKENHAC_00004 4298 4 Oral 0.40 protein_coding synonymous_variant LOW 306A>T Leu102Leu
M0124470 MLKENHAC_00004 4514 4 Oral 0.40 protein_coding synonymous_variant LOW 522T>C Asp174Asp
M0124471 MLKENHAC_00004 4556 3 Oral 0.30 protein_coding synonymous_variant LOW 564A>G Leu188Leu
M0124472 MLKENHAC_00004 4568 5 Oral 0.50 protein_coding synonymous_variant LOW 576T>C Tyr192Tyr
M0124473 MLKENHAC_00004 4593 5 Oral 0.50 protein_coding missense_variant MODERATE 601A>G Ile201Val
M0124474 MLKENHAC_00004 4673 3 Oral 0.30 protein_coding synonymous_variant LOW 681A>G Pro227Pro
M0124475 MLKENHAC_00004 4729 3 Oral 0.30 protein_coding missense_variant MODERATE 737A>G His246Arg
M0124476 MLKENHAC_00004 4826 3 Oral 0.30 protein_coding synonymous_variant LOW 834A>C Ile278Ile
M0124477 MLKENHAC_00004 4832 5 Oral 0.50 protein_coding synonymous_variant LOW 840T>G Ser280Ser
M0124478 MLKENHAC_00004 4886 4 Oral 0.40 protein_coding synonymous_variant LOW 894G>A Val298Val
M0124479 MLKENHAC_00004 4898 3 Oral 0.30 protein_coding synonymous_variant LOW 906T>A Val302Val
M0124480 MLKENHAC_00004 4916 3 Oral 0.30 protein_coding synonymous_variant LOW 924A>G Glu308Glu
M0124481 MLKENHAC_00004 4985 3 Oral 0.30 protein_coding synonymous_variant LOW 993G>A Leu331Leu
M0124482 MLKENHAC_00004 5000 5 Oral 0.50 protein_coding synonymous_variant LOW 1008C>T Asn336Asn
M0124483 MLKENHAC_00004 5255 3 Oral 0.30 protein_coding synonymous_variant LOW 1263G>T Thr421Thr
M0124484 MLKENHAC_00004 5294 3 Oral 0.30 protein_coding synonymous_variant LOW 1302T>C Tyr434Tyr
M0124485 MLKENHAC_00004 5327 3 Oral 0.30 protein_coding synonymous_variant LOW 1335C>T Tyr445Tyr
M0124486 MLKENHAC_00004 5330 3 Oral 0.30 protein_coding synonymous_variant LOW 1338C>T Ser446Ser
M0124487 MLKENHAC_00004 5474 3 Oral 0.30 protein_coding synonymous_variant LOW 1482A>G Val494Val
M0124488 MLKENHAC_00004 5535 4 Oral 0.40 protein_coding missense_variant MODERATE 1543G>A Ala515Thr
M0124489 MLKENHAC_00004 5544 4 Oral 0.40 protein_coding missense_variant MODERATE 1552A>G Ile518Val
M0124490 MLKENHAC_00004 5954 3 Oral 0.30 protein_coding synonymous_variant LOW 1962T>C His654His
M0124491 MLKENHAC_00004 5957 4 Oral 0.40 protein_coding synonymous_variant LOW 1965G>A Gln655Gln
M0124492 MLKENHAC_00007 7363 3 Oral 0.30 protein_coding missense_variant MODERATE 932T>A Ile311Asn
M0124493 MLKENHAC_00007 7518 3 Oral 0.30 protein_coding synonymous_variant LOW 777T>A Thr259Thr
M0124494 MLKENHAC_00007 7554 3 Oral 0.30 protein_coding synonymous_variant LOW 741A>G Glu247Glu
M0124495 MLKENHAC_00007 7841 4 Oral 0.40 protein_coding missense_variant MODERATE 454A>G Ile152Val
M0124496 MLKENHAC_00007 7977 4 Oral 0.40 protein_coding synonymous_variant LOW 318G>A Ala106Ala
M0124497 MLKENHAC_00007 8115 3 Oral 0.30 protein_coding synonymous_variant LOW 180A>G Leu60Leu
M0124498 MLKENHAC_00007 8194 5 Oral 0.50 protein_coding missense_variant MODERATE 101A>G Asn34Ser
M0124499 MLKENHAC_00003 8337 4 Oral 0.40 protein_coding upstream_gene_variant MODIFIER -4405G>A None
M0124500 MLKENHAC_00003 8347 3 Oral 0.30 protein_coding upstream_gene_variant MODIFIER -4415C>T None
M0124501 MLKENHAC_00008 8413 3 Oral 0.30 protein_coding synonymous_variant LOW 357G>A Glu119Glu
M0124502 MLKENHAC_00008 8470 4 Oral 0.40 protein_coding synonymous_variant LOW 300T>C Tyr100Tyr
M0124503 MLKENHAC_00008 8479 3 Oral 0.30 protein_coding synonymous_variant LOW 291A>G Glu97Glu
M0124504 MLKENHAC_00008 8551 3 Oral 0.30 protein_coding synonymous_variant LOW 219T>A Thr73Thr
M0124505 MLKENHAC_00008 8626 3 Oral 0.30 protein_coding synonymous_variant LOW 144A>C Ala48Ala
M0124506 MLKENHAC_00007 7680 3 Oral 0.30 protein_coding synonymous_variant LOW 615T>C Leu205Leu
M0124507 MLKENHAC_00007 7689 3 Oral 0.30 protein_coding synonymous_variant LOW 606A>C Ile202Ile
M0124508 MLKENHAC_00007 7710 3 Oral 0.30 protein_coding missense_variant MODERATE 585G>T Glu195Asp
M0124509 MLKENHAC_00007 7739 3 Oral 0.30 protein_coding missense_variant MODERATE 556T>A Ser186Thr
M0124510 MLKENHAC_00007 7745 3 Oral 0.30 protein_coding synonymous_variant LOW 550T>C Leu184Leu
M0124511 MLKENHAC_00007 7857 3 Oral 0.30 protein_coding synonymous_variant LOW 438A>G Thr146Thr
M0124512 MLKENHAC_00007 7979 3 Oral 0.30 protein_coding missense_variant MODERATE 316G>A Ala106Thr
M0124513 MLKENHAC_00007 8103 4 Oral 0.40 protein_coding synonymous_variant LOW 192T>C Pro64Pro
M0124514 MLKENHAC_00003 8313 3 Oral 0.30 protein_coding upstream_gene_variant MODIFIER -4381T>C None
M0124515 MLKENHAC_00003 8323 3 Oral 0.30 protein_coding upstream_gene_variant MODIFIER -4391C>G None
M0124516 MLKENHAC_00003 8331 3 Oral 0.30 protein_coding upstream_gene_variant MODIFIER -4399T>A None
M0124517 MLKENHAC_00003 8333 3 Oral 0.30 protein_coding upstream_gene_variant MODIFIER -4401A>G None
M0124518 MLKENHAC_00004 4086 3 Oral 0.30 protein_coding synonymous_variant LOW 94T>C Leu32Leu
M0124519 MLKENHAC_00004 4277 3 Oral 0.30 protein_coding synonymous_variant LOW 285C>T Phe95Phe
M0124520 MLKENHAC_00004 4511 4 Oral 0.40 protein_coding synonymous_variant LOW 519T>C Asp173Asp
M0124521 MLKENHAC_00004 4997 3 Oral 0.30 protein_coding synonymous_variant LOW 1005T>G Ala335Ala






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term