Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C2484
  Reference Plasmid   1111525849768216_bin.27__k141_1482746
  Reference Plasmid Size   17659
  Reference Plasmid GC Content   0.70
  Reference Plasmid Mobility Type   non-mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0041397 PKAEECDC_00013 15525 6 Skin 0.12 protein_coding synonymous_variant LOW 840G>C Ala280Ala
M0041398 PKAEECDC_00013 15749 4 Skin 0.08 protein_coding missense_variant MODERATE 616C>A Leu206Ile
M0041399 PKAEECDC_00013 15759 4 Skin 0.08 protein_coding synonymous_variant LOW 606T>C Val202Val
M0041400 PKAEECDC_00013 15810 3 Skin 0.06 protein_coding missense_variant MODERATE 555G>C Glu185Asp
M0041401 PKAEECDC_00013 15837 7 Skin 0.13 protein_coding synonymous_variant LOW 528C>G Arg176Arg
M0041402 PKAEECDC_00013 15891 4 Skin 0.08 protein_coding synonymous_variant LOW 474G>C Val158Val
M0041403 PKAEECDC_00013 15951 6 Skin 0.12 protein_coding synonymous_variant LOW 414C>T Arg138Arg
M0041404 PKAEECDC_00013 15978 5 Skin 0.10 protein_coding synonymous_variant LOW 387T>G Leu129Leu
M0041405 PKAEECDC_00013 15993 5 Skin 0.10 protein_coding missense_variant MODERATE 372A>C Glu124Asp
M0041406 PKAEECDC_00013 16056 6 Skin 0.12 protein_coding synonymous_variant LOW 309C>G Thr103Thr
M0041407 PKAEECDC_00013 16095 6 Skin 0.12 protein_coding synonymous_variant LOW 270A>G Gly90Gly
M0041408 PKAEECDC_00013 16119 5 Skin 0.10 protein_coding synonymous_variant LOW 246G>C Arg82Arg
M0041409 PKAEECDC_00013 16218 4 Skin 0.08 protein_coding synonymous_variant LOW 147C>A Thr49Thr
M0041410 PKAEECDC_00013 16221 4 Skin 0.08 protein_coding synonymous_variant LOW 144T>C Asp48Asp
M0041411 PKAEECDC_00013 16242 3 Skin 0.06 protein_coding synonymous_variant LOW 123G>T Pro41Pro
M0041412 PKAEECDC_00013 16263 3 Skin 0.06 protein_coding synonymous_variant LOW 102A>G Ala34Ala
M0041413 PKAEECDC_00013 16293 5 Skin 0.10 protein_coding synonymous_variant LOW 72C>G Ser24Ser
M0041414 PKAEECDC_00003 3219 14 Skin 0.27 protein_coding upstream_gene_variant MODIFIER -475A>C None
M0041415 PKAEECDC_00005 5223 19 Skin 0.37 protein_coding missense_variant MODERATE 965G>T Gly322Val
M0041416 PKAEECDC_00005 5506 17 Skin 0.33 protein_coding missense_variant MODERATE 682A>G Asn228Asp
M0041417 PKAEECDC_00013 15087 6 Skin 0.12 protein_coding synonymous_variant LOW 1278C>T Ile426Ile
M0041418 PKAEECDC_00013 15186 7 Skin 0.13 protein_coding synonymous_variant LOW 1179C>G Ser393Ser
M0041419 PKAEECDC_00013 15207 7 Skin 0.13 protein_coding synonymous_variant LOW 1158G>T Leu386Leu
M0041420 PKAEECDC_00013 15210 7 Skin 0.13 protein_coding synonymous_variant LOW 1155C>T Ser385Ser
M0041421 PKAEECDC_00009 10240 4 Skin 0.08 protein_coding synonymous_variant LOW 480T>C Phe160Phe
M0041422 PKAEECDC_00005 4010 4 Skin 0.08 protein_coding synonymous_variant LOW 2178T>C Asn726Asn
M0041423 PKAEECDC_00005 5030 3 Skin 0.06 protein_coding synonymous_variant LOW 1158G>A Glu386Glu
M0041424 PKAEECDC_00005 6161 3 Skin 0.06 protein_coding missense_variant MODERATE 27C>G Asp9Glu
M0041425 PKAEECDC_00003 2370 3 Skin 0.06 protein_coding synonymous_variant LOW 375T>C Asp125Asp
M0041426 PKAEECDC_00009 10450 3 Skin 0.06 protein_coding synonymous_variant LOW 270C>A Ala90Ala
M0041427 PKAEECDC_00009 10474 3 Skin 0.06 protein_coding synonymous_variant LOW 246G>T Gly82Gly
M0041428 PKAEECDC_00009 10549 3 Skin 0.06 protein_coding synonymous_variant LOW 171C>G Ala57Ala
M0041429 PKAEECDC_00012 14465 4 Skin 0.08 protein_coding missense_variant MODERATE 269G>A Arg90His
M0041430 PKAEECDC_00012 14488 5 Skin 0.10 protein_coding synonymous_variant LOW 246A>G Glu82Glu
M0041431 PKAEECDC_00009 14843 3 Skin 0.06 protein_coding upstream_gene_variant MODIFIER -4124G>C None
M0041432 PKAEECDC_00009 14848 4 Skin 0.08 protein_coding upstream_gene_variant MODIFIER -4129G>C None
M0041433 PKAEECDC_00009 14849 4 Skin 0.08 protein_coding upstream_gene_variant MODIFIER -4130T>C None
M0041434 PKAEECDC_00009 14850 4 Skin 0.08 protein_coding upstream_gene_variant MODIFIER -4131G>T None
M0041435 PKAEECDC_00009 14852 3 Skin 0.06 protein_coding upstream_gene_variant MODIFIER -4133G>A None
M0041436 PKAEECDC_00009 14853 4 Skin 0.08 protein_coding upstream_gene_variant MODIFIER -4134T>C None
M0041437 PKAEECDC_00009 14904 4 Skin 0.08 protein_coding upstream_gene_variant MODIFIER -4185C>G None
M0041438 PKAEECDC_00013 15066 4 Skin 0.08 protein_coding synonymous_variant LOW 1299G>T Leu433Leu
M0041439 PKAEECDC_00013 15141 4 Skin 0.08 protein_coding synonymous_variant LOW 1224C>G Val408Val
M0041440 PKAEECDC_00013 15144 4 Skin 0.08 protein_coding synonymous_variant LOW 1221G>C Leu407Leu
M0041441 PKAEECDC_00012 14098 3 Skin 0.06 protein_coding synonymous_variant LOW 636T>C Gly212Gly
M0041442 PKAEECDC_00012 14119 3 Skin 0.06 protein_coding synonymous_variant LOW 615A>C Ile205Ile
M0041443 PKAEECDC_00008 8872 4 Skin 0.08 protein_coding missense_variant MODERATE 554A>G Asp185Gly
M0041444 PKAEECDC_00009 9712 4 Skin 0.08 protein_coding synonymous_variant LOW 1008T>C Arg336Arg
M0041445 PKAEECDC_00009 9716 4 Skin 0.08 protein_coding missense_variant MODERATE 1004T>C Ile335Thr
M0041446 PKAEECDC_00009 10684 3 Skin 0.06 protein_coding synonymous_variant LOW 36C>A Ala12Ala
M0041447 PKAEECDC_00009 10708 4 Skin 0.08 protein_coding synonymous_variant LOW 12G>A Gln4Gln
M0041448 PKAEECDC_00012 13816 3 Skin 0.06 protein_coding synonymous_variant LOW 918T>C Ala306Ala
M0041449 PKAEECDC_00012 13843 3 Skin 0.06 protein_coding synonymous_variant LOW 891T>C Pro297Pro
M0041450 PKAEECDC_00014 16702 3 Skin 0.06 protein_coding missense_variant MODERATE 520C>T Arg174Cys
M0041451 PKAEECDC_00014 16777 3 Skin 0.06 protein_coding missense_variant MODERATE 445C>G His149Asp
M0041452 PKAEECDC_00014 16784 3 Skin 0.06 protein_coding synonymous_variant LOW 438G>T Thr146Thr
M0041453 PKAEECDC_00014 16785 3 Skin 0.06 protein_coding missense_variant MODERATE 437C>T Thr146Met
M0041454 PKAEECDC_00014 16786 3 Skin 0.06 protein_coding missense_variant MODERATE 436A>C Thr146Pro
M0041455 PKAEECDC_00014 16916 3 Skin 0.06 protein_coding synonymous_variant LOW 306C>T Arg102Arg
M0041456 PKAEECDC_00008 8576 4 Skin 0.08 protein_coding missense_variant MODERATE 850T>A Phe284Ile
M0041457 PKAEECDC_00010 11943 3 Skin 0.06 protein_coding synonymous_variant LOW 339C>T Tyr113Tyr
M0041458 PKAEECDC_00010 12033 8 Skin 0.15 protein_coding synonymous_variant LOW 249G>T Arg83Arg
M0041459 PKAEECDC_00012 14167 3 Skin 0.06 protein_coding synonymous_variant LOW 567A>C Arg189Arg
M0041460 PKAEECDC_00012 14638 3 Skin 0.06 protein_coding synonymous_variant LOW 96G>A Ala32Ala
M0041461 PKAEECDC_00010 12048 3 Skin 0.06 protein_coding synonymous_variant LOW 234C>G Gly78Gly
M0041462 PKAEECDC_00010 12075 8 Skin 0.15 protein_coding synonymous_variant LOW 207A>G Ala69Ala
M0041463 PKAEECDC_00005 9511 3 Skin 0.06 protein_coding upstream_gene_variant MODIFIER -3324G>C None
M0041464 PKAEECDC_00005 9568 3 Skin 0.06 protein_coding upstream_gene_variant MODIFIER -3381G>T None
M0041465 PKAEECDC_00005 9569 3 Skin 0.06 protein_coding upstream_gene_variant MODIFIER -3382C>G None
M0041466 PKAEECDC_00009 10054 3 Skin 0.06 protein_coding missense_variant MODERATE 666G>C Glu222Asp
M0041467 PKAEECDC_00009 10099 3 Skin 0.06 protein_coding synonymous_variant LOW 621T>C Tyr207Tyr
M0041468 PKAEECDC_00009 10131 3 Skin 0.06 protein_coding synonymous_variant LOW 589A>C Arg197Arg
M0041469 PKAEECDC_00009 10144 3 Skin 0.06 protein_coding synonymous_variant LOW 576T>G Leu192Leu
M0041470 PKAEECDC_00009 10189 3 Skin 0.06 protein_coding synonymous_variant LOW 531T>C Phe177Phe
M0041471 PKAEECDC_00007 8211 3 Skin 0.06 protein_coding missense_variant MODERATE 19C>A Leu7Ile
M0041472 PKAEECDC_00004 8233 3 Skin 0.06 protein_coding upstream_gene_variant MODIFIER -4632A>T None
M0041473 PKAEECDC_00008 8725 3 Skin 0.06 protein_coding missense_variant MODERATE 701A>C Lys234Thr
M0041474 PKAEECDC_00008 8739 3 Skin 0.06 protein_coding missense_variant MODERATE 687G>T Glu229Asp
M0041475 PKAEECDC_00008 8778 3 Skin 0.06 protein_coding synonymous_variant LOW 648C>T Arg216Arg
M0041476 PKAEECDC_00008 8892 3 Skin 0.06 protein_coding synonymous_variant LOW 534T>C Pro178Pro
M0041477 PKAEECDC_00009 10369 3 Skin 0.06 protein_coding synonymous_variant LOW 351C>G Leu117Leu
M0041478 PKAEECDC_00009 10402 3 Skin 0.06 protein_coding synonymous_variant LOW 318C>T Asp106Asp
M0041479 PKAEECDC_00009 10408 4 Skin 0.08 protein_coding synonymous_variant LOW 312C>G Ala104Ala
M0041480 PKAEECDC_00005 10767 3 Skin 0.06 protein_coding upstream_gene_variant MODIFIER -4580G>A None
M0041481 PKAEECDC_00005 10786 3 Skin 0.06 protein_coding upstream_gene_variant MODIFIER -4599C>G None
M0041482 PKAEECDC_00005 10810 3 Skin 0.06 protein_coding upstream_gene_variant MODIFIER -4623T>C None
M0041483 PKAEECDC_00005 10811 3 Skin 0.06 protein_coding upstream_gene_variant MODIFIER -4624T>G None
M0041484 PKAEECDC_00005 10812 3 Skin 0.06 protein_coding upstream_gene_variant MODIFIER -4625G>C None
M0041485 PKAEECDC_00005 10816 3 Skin 0.06 protein_coding upstream_gene_variant MODIFIER -4629C>T None
M0041486 PKAEECDC_00005 10817 3 Skin 0.06 protein_coding upstream_gene_variant MODIFIER -4630C>T None
M0041487 PKAEECDC_00005 10819 3 Skin 0.06 protein_coding upstream_gene_variant MODIFIER -4632C>G None
M0041488 PKAEECDC_00010 11210 7 Skin 0.13 protein_coding missense_variant MODERATE 1072A>G Ile358Val
M0041489 PKAEECDC_00010 12234 3 Skin 0.06 protein_coding synonymous_variant LOW 48C>G Pro16Pro
M0041490 PKAEECDC_00007 12312 3 Skin 0.06 protein_coding upstream_gene_variant MODIFIER -4083T>C None
M0041491 PKAEECDC_00007 12313 3 Skin 0.06 protein_coding upstream_gene_variant MODIFIER -4084T>C None
M0041492 PKAEECDC_00010 11781 5 Skin 0.10 protein_coding synonymous_variant LOW 501C>T Asp167Asp
M0041493 PKAEECDC_00010 11211 4 Skin 0.08 protein_coding synonymous_variant LOW 1071G>C Leu357Leu






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
PKAEECDC_00007 QXI66128.1|GT2 73.9 7.51e-160 9 326 0.955 0.9666
PKAEECDC_00008 QXI66129.1|GT0 74 1.65e-195 1 388 0.9823 0.9662
PKAEECDC_00012 QQO46796.1|GT4 70.9 2.18e-176 1 350 0.9887 0.9777





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term