Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C2489
  Reference Plasmid   1111525849770636_bin.4__k141_302624
  Reference Plasmid Size   2514
  Reference Plasmid GC Content   0.31
  Reference Plasmid Mobility Type   non-mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0041500 EBKNPFDD_00001 175 4 Skin 0.40 protein_coding upstream_gene_variant MODIFIER -554T>C None
M0041501 EBKNPFDD_00001 433 3 Skin 0.30 protein_coding upstream_gene_variant MODIFIER -296A>G None
M0041502 EBKNPFDD_00001 445 3 Skin 0.30 protein_coding upstream_gene_variant MODIFIER -284A>G None
M0041503 EBKNPFDD_00001 496 3 Skin 0.30 protein_coding upstream_gene_variant MODIFIER -233T>C None
M0041504 EBKNPFDD_00001 571 4 Skin 0.40 protein_coding upstream_gene_variant MODIFIER -158A>T None
M0041505 EBKNPFDD_00001 573 4 Skin 0.40 protein_coding upstream_gene_variant MODIFIER -156T>A None
M0041506 EBKNPFDD_00001 761 5 Skin 0.50 protein_coding synonymous_variant LOW 33A>G Glu11Glu
M0041507 EBKNPFDD_00001 846 4 Skin 0.40 protein_coding missense_variant MODERATE 118C>A Gln40Lys
M0041508 EBKNPFDD_00001 852 5 Skin 0.50 protein_coding missense_variant MODERATE 124G>A Glu42Lys
M0041509 EBKNPFDD_00001 905 4 Skin 0.40 protein_coding synonymous_variant LOW 177C>T Ile59Ile
M0041510 EBKNPFDD_00001 1035 5 Skin 0.50 protein_coding missense_variant MODERATE 307A>G Lys103Glu
M0041511 EBKNPFDD_00001 1040 6 Skin 0.60 protein_coding synonymous_variant LOW 312A>G Val104Val
M0041512 EBKNPFDD_00001 1103 5 Skin 0.50 protein_coding missense_variant MODERATE 375T>A Asn125Lys
M0041513 EBKNPFDD_00001 1109 5 Skin 0.50 protein_coding synonymous_variant LOW 381T>C Cys127Cys
M0041514 EBKNPFDD_00001 1154 6 Skin 0.60 protein_coding synonymous_variant LOW 426A>G Glu142Glu
M0041515 EBKNPFDD_00002 1227 7 Skin 0.70 protein_coding upstream_gene_variant MODIFIER -69A>T None
M0041516 EBKNPFDD_00002 1523 6 Skin 0.60 protein_coding synonymous_variant LOW 228G>A Ala76Ala
M0041517 EBKNPFDD_00002 1532 5 Skin 0.50 protein_coding missense_variant MODERATE 237C>G Asp79Glu
M0041518 EBKNPFDD_00002 1565 4 Skin 0.40 protein_coding synonymous_variant LOW 270T>C Phe90Phe
M0041519 EBKNPFDD_00002 1644 6 Skin 0.60 protein_coding missense_variant MODERATE 349A>G Ile117Val
M0041520 EBKNPFDD_00002 1661 4 Skin 0.40 protein_coding synonymous_variant LOW 366C>T Ile122Ile
M0041521 EBKNPFDD_00002 1663 6 Skin 0.60 protein_coding missense_variant MODERATE 368C>A Thr123Asn
M0041522 EBKNPFDD_00002 1682 6 Skin 0.60 protein_coding synonymous_variant LOW 387C>T Asn129Asn
M0041523 EBKNPFDD_00002 1685 6 Skin 0.60 protein_coding synonymous_variant LOW 390T>A Val130Val
M0041524 EBKNPFDD_00002 1721 6 Skin 0.60 protein_coding synonymous_variant LOW 426A>G Thr142Thr
M0041525 EBKNPFDD_00002 1922 5 Skin 0.50 protein_coding synonymous_variant LOW 627C>T Ser209Ser
M0041526 EBKNPFDD_00002 1953 5 Skin 0.50 protein_coding missense_variant MODERATE 658A>G Lys220Glu
M0041527 EBKNPFDD_00002 1961 6 Skin 0.60 protein_coding synonymous_variant LOW 666G>A Pro222Pro
M0041528 EBKNPFDD_00002 1991 5 Skin 0.50 protein_coding synonymous_variant LOW 696A>C Ile232Ile
M0041529 EBKNPFDD_00002 2024 5 Skin 0.50 protein_coding synonymous_variant LOW 729C>G Thr243Thr
M0041530 EBKNPFDD_00002 2120 5 Skin 0.50 protein_coding synonymous_variant LOW 825T>G Arg275Arg
M0041531 EBKNPFDD_00002 2121 5 Skin 0.50 protein_coding missense_variant MODERATE 826A>G Asn276Asp
M0041532 EBKNPFDD_00002 2133 5 Skin 0.50 protein_coding missense_variant MODERATE 838G>A Ala280Thr
M0041533 EBKNPFDD_00002 2135 6 Skin 0.60 protein_coding synonymous_variant LOW 840A>G Ala280Ala
M0041534 EBKNPFDD_00002 2144 5 Skin 0.50 protein_coding synonymous_variant LOW 849G>A Lys283Lys
M0041535 EBKNPFDD_00002 2198 5 Skin 0.50 protein_coding synonymous_variant LOW 903G>A Val301Val
M0041536 EBKNPFDD_00002 2282 4 Skin 0.40 protein_coding synonymous_variant LOW 987G>A Gly329Gly
M0041537 EBKNPFDD_00002 2291 4 Skin 0.40 protein_coding synonymous_variant LOW 996A>T Gly332Gly
M0041538 EBKNPFDD_00002 2330 6 Skin 0.60 protein_coding synonymous_variant LOW 1035G>A Gly345Gly
M0041539 EBKNPFDD_00002 2416 5 Skin 0.50 protein_coding missense_variant MODERATE 1121C>A Thr374Lys
M0041540 EBKNPFDD_00002 2445 4 Skin 0.40 protein_coding missense_variant MODERATE 1150A>G Arg384Gly
M0041541 EBKNPFDD_00001 2460 4 Skin 0.40 protein_coding downstream_gene_variant MODIFIER *1291G>A None
M0041542 EBKNPFDD_00002 1280 4 Skin 0.40 protein_coding upstream_gene_variant MODIFIER -16A>G None
M0041543 EBKNPFDD_00001 409 4 Skin 0.40 protein_coding upstream_gene_variant MODIFIER -320C>T None
M0041544 EBKNPFDD_00001 983 5 Skin 0.50 protein_coding synonymous_variant LOW 255G>A Ser85Ser
M0041545 EBKNPFDD_00002 1634 4 Skin 0.40 protein_coding synonymous_variant LOW 339T>G Gly113Gly
M0041546 EBKNPFDD_00002 1811 5 Skin 0.50 protein_coding synonymous_variant LOW 516C>T Ser172Ser
M0041547 EBKNPFDD_00001 109 4 Skin 0.40 protein_coding upstream_gene_variant MODIFIER -620T>C None
M0041548 EBKNPFDD_00001 319 3 Skin 0.30 protein_coding upstream_gene_variant MODIFIER -410T>C None






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term