Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C2543
  Reference Plasmid   1111525849852267_bin.38__k141_421253
  Reference Plasmid Size   27627
  Reference Plasmid GC Content   0.57
  Reference Plasmid Mobility Type   non-mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0046929 MKGHNCHK_00007 11625 9 Skin 0.38 protein_coding missense_variant MODERATE 380A>G Asp127Gly
M0046930 MKGHNCHK_00007 11713 9 Skin 0.38 protein_coding missense_variant MODERATE 292C>G Pro98Ala
M0046931 MKGHNCHK_00011 15731 6 Skin 0.25 protein_coding missense_variant MODERATE 355G>A Val119Ile
M0046932 MKGHNCHK_00020 24609 9 Skin 0.38 protein_coding synonymous_variant LOW 921G>T Gly307Gly
M0046933 MKGHNCHK_00006 9226 9 Skin 0.38 protein_coding missense_variant MODERATE 1421A>G Asp474Gly
M0046934 MKGHNCHK_00014 23977 5 Skin 0.21 protein_coding upstream_gene_variant MODIFIER -4192G>A None
M0046935 MKGHNCHK_00014 23988 5 Skin 0.21 protein_coding upstream_gene_variant MODIFIER -4203G>A None
M0046936 MKGHNCHK_00014 23990 5 Skin 0.21 protein_coding upstream_gene_variant MODIFIER -4205A>G None






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant
MKGHNCHK_00003 PHI:10051 glgA 92.2 2.7e-266 1 476 0.9979 0.9979 rodents salmonellosis glycogen synthase reduced virulence






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
MKGHNCHK_00002 QIU90803.1|GT35 99.9 0 1 815 1 1
MKGHNCHK_00003 QIU90804.1|GT5 100 0 1 477 1 1
MKGHNCHK_00004 VDZ68364.1|GH13_11 94.4 7.87e-303 1 431 1 0.738
MKGHNCHK_00005 QIU90806.1|CBM48|GH13_11 99.8 0 1 658 1 1
MKGHNCHK_00006 QIU90807.1|CBM48|GH13_9 99.9 0 1 728 1 1





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
MKGHNCHK_00008 2.A.8.1.8 96.4 1.4e-237 1 446 1.0000 1.0000 2 Electrochemical Potential-driven Transporters 2.A Porters (uniporters, symporters, antiporters) 2.A.8 The Gluconate:H+ Symporter (GntP) Family
MKGHNCHK_00017 3.A.1.1.3 87.7 1.7e-180 1 357 1.0000 1.0000 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.1 The ATP-binding Cassette (ABC) Superfamily
MKGHNCHK_00018 3.A.1.1.3 88.3 1.7e-135 1 281 1.0000 0.7893 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.1 The ATP-binding Cassette (ABC) Superfamily
MKGHNCHK_00019 3.A.1.1.3 92.9 4.2e-153 1 295 1.0000 0.8287 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.1 The ATP-binding Cassette (ABC) Superfamily
MKGHNCHK_00020 3.A.1.1.3 88.8 5.5e-234 1 438 1.0000 1.2303 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.1 The ATP-binding Cassette (ABC) Superfamily
MKGHNCHK_00021 3.A.1.4.1 95.3 3.6e-123 1 233 1.0000 0.9831 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.1 The ATP-binding Cassette (ABC) Superfamily
MKGHNCHK_00022 3.A.1.4.1 94.9 3.7e-137 1 255 1.0000 1.0759 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.1 The ATP-binding Cassette (ABC) Superfamily