Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C2589
  Reference Plasmid   1111525849854152_bin.43__k141_178805
  Reference Plasmid Size   21755
  Reference Plasmid GC Content   0.64
  Reference Plasmid Mobility Type   mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0048201 ENHDLEAH_00007 6121 5 Skin 0.36 protein_coding missense_variant MODERATE 1132G>A Val378Ile
M0048202 ENHDLEAH_00010 8639 3 Skin 0.21 protein_coding missense_variant MODERATE 1037A>C Glu346Ala
M0048203 ENHDLEAH_00010 11324 3 Skin 0.21 protein_coding upstream_gene_variant MODIFIER -1649G>T None
M0048204 ENHDLEAH_00013 12469 4 Skin 0.29 protein_coding synonymous_variant LOW 772T>C Leu258Leu
M0048205 ENHDLEAH_00013 12480 4 Skin 0.29 protein_coding synonymous_variant LOW 783A>G Ser261Ser
M0048206 ENHDLEAH_00013 12487 3 Skin 0.21 protein_coding synonymous_variant LOW 790T>C Leu264Leu
M0048207 ENHDLEAH_00013 12519 4 Skin 0.29 protein_coding synonymous_variant LOW 822T>C Arg274Arg
M0048208 ENHDLEAH_00014 14242 3 Skin 0.21 protein_coding missense_variant MODERATE 1217T>C Val406Ala
M0048209 ENHDLEAH_00015 15861 6 Skin 0.43 protein_coding synonymous_variant LOW 1227T>A Gly409Gly
M0048210 ENHDLEAH_00015 16248 3 Skin 0.21 protein_coding synonymous_variant LOW 1614A>C Ala538Ala
M0048211 ENHDLEAH_00015 16252 3 Skin 0.21 protein_coding missense_variant MODERATE 1618A>G Met540Val
M0048212 ENHDLEAH_00015 16305 3 Skin 0.21 protein_coding synonymous_variant LOW 1671A>C Arg557Arg
M0048213 ENHDLEAH_00015 16395 3 Skin 0.21 protein_coding synonymous_variant LOW 1761T>G Val587Val
M0048214 ENHDLEAH_00015 16419 3 Skin 0.21 protein_coding synonymous_variant LOW 1785C>G Ala595Ala
M0048215 ENHDLEAH_00007 5251 3 Skin 0.21 protein_coding missense_variant MODERATE 262G>C Glu88Gln
M0048216 ENHDLEAH_00007 5259 3 Skin 0.21 protein_coding synonymous_variant LOW 270A>G Leu90Leu
M0048217 ENHDLEAH_00015 15762 3 Skin 0.21 protein_coding synonymous_variant LOW 1128T>C Pro376Pro
M0048218 ENHDLEAH_00014 13794 4 Skin 0.29 protein_coding missense_variant MODERATE 769C>G Leu257Val
M0048219 ENHDLEAH_00014 13864 3 Skin 0.21 protein_coding missense_variant MODERATE 839G>T Arg280Leu
M0048220 ENHDLEAH_00014 13868 3 Skin 0.21 protein_coding synonymous_variant LOW 843A>C Ala281Ala
M0048221 ENHDLEAH_00011 10245 3 Skin 0.21 protein_coding synonymous_variant LOW 246T>C Arg82Arg
M0048222 ENHDLEAH_00010 10559 4 Skin 0.29 protein_coding upstream_gene_variant MODIFIER -884C>A None
M0048223 ENHDLEAH_00010 10609 4 Skin 0.29 protein_coding upstream_gene_variant MODIFIER -934A>G None
M0048224 ENHDLEAH_00013 11756 3 Skin 0.21 protein_coding missense_variant MODERATE 59T>C Val20Ala
M0048225 ENHDLEAH_00013 11859 3 Skin 0.21 protein_coding synonymous_variant LOW 162T>C Thr54Thr
M0048226 ENHDLEAH_00013 11904 3 Skin 0.21 protein_coding synonymous_variant LOW 207A>G Leu69Leu
M0048227 ENHDLEAH_00013 11922 3 Skin 0.21 protein_coding synonymous_variant LOW 225A>G Glu75Glu
M0048228 ENHDLEAH_00013 11931 3 Skin 0.21 protein_coding synonymous_variant LOW 234G>C Ala78Ala
M0048229 ENHDLEAH_00013 12132 3 Skin 0.21 protein_coding synonymous_variant LOW 435T>C Val145Val
M0048230 ENHDLEAH_00013 12198 3 Skin 0.21 protein_coding synonymous_variant LOW 501G>A Leu167Leu
M0048231 ENHDLEAH_00013 12306 3 Skin 0.21 protein_coding synonymous_variant LOW 609G>A Glu203Glu
M0048232 ENHDLEAH_00013 12372 3 Skin 0.21 protein_coding synonymous_variant LOW 675C>T Ala225Ala
M0048233 ENHDLEAH_00013 12426 3 Skin 0.21 protein_coding synonymous_variant LOW 729C>T Asp243Asp
M0048234 ENHDLEAH_00013 12516 3 Skin 0.21 protein_coding synonymous_variant LOW 819C>T Arg273Arg
M0048235 ENHDLEAH_00013 12670 3 Skin 0.21 protein_coding synonymous_variant LOW 973T>C Leu325Leu
M0048236 ENHDLEAH_00013 12711 3 Skin 0.21 protein_coding synonymous_variant LOW 1014C>T Leu338Leu
M0048237 ENHDLEAH_00013 12734 3 Skin 0.21 protein_coding missense_variant MODERATE 1037A>G Asn346Ser
M0048238 ENHDLEAH_00013 12742 3 Skin 0.21 protein_coding missense_variant MODERATE 1045C>G Leu349Val
M0048239 ENHDLEAH_00013 12790 3 Skin 0.21 protein_coding missense_variant MODERATE 1093A>G Ile365Val
M0048240 ENHDLEAH_00013 12872 3 Skin 0.21 protein_coding missense_variant MODERATE 1175A>T Tyr392Phe
M0048241 ENHDLEAH_00013 12873 3 Skin 0.21 protein_coding synonymous_variant LOW 1176C>T Tyr392Tyr
M0048242 ENHDLEAH_00013 12921 3 Skin 0.21 protein_coding synonymous_variant LOW 1224G>A Gln408Gln
M0048243 ENHDLEAH_00014 13056 3 Skin 0.21 protein_coding missense_variant MODERATE 31G>A Gly11Ser
M0048244 ENHDLEAH_00014 13154 3 Skin 0.21 protein_coding synonymous_variant LOW 129C>T Gly43Gly
M0048245 ENHDLEAH_00014 13175 3 Skin 0.21 protein_coding synonymous_variant LOW 150T>G Val50Val
M0048246 ENHDLEAH_00014 14430 3 Skin 0.21 protein_coding missense_variant MODERATE 1405A>G Arg469Gly
M0048247 ENHDLEAH_00010 14630 3 Skin 0.21 protein_coding upstream_gene_variant MODIFIER -4955A>G None
M0048248 ENHDLEAH_00015 14691 4 Skin 0.29 protein_coding synonymous_variant LOW 57C>T Ala19Ala
M0048249 ENHDLEAH_00015 14751 3 Skin 0.21 protein_coding synonymous_variant LOW 117A>G Val39Val
M0048250 ENHDLEAH_00015 14754 3 Skin 0.21 protein_coding synonymous_variant LOW 120C>G Pro40Pro
M0048251 ENHDLEAH_00015 14832 3 Skin 0.21 protein_coding synonymous_variant LOW 198T>C Thr66Thr
M0048252 ENHDLEAH_00015 14835 3 Skin 0.21 protein_coding synonymous_variant LOW 201T>C Tyr67Tyr
M0048253 ENHDLEAH_00015 15099 3 Skin 0.21 protein_coding synonymous_variant LOW 465G>C Gly155Gly
M0048254 ENHDLEAH_00015 15357 3 Skin 0.21 protein_coding synonymous_variant LOW 723C>T Pro241Pro
M0048255 ENHDLEAH_00015 15360 3 Skin 0.21 protein_coding synonymous_variant LOW 726A>G Gly242Gly
M0048256 ENHDLEAH_00015 15378 3 Skin 0.21 protein_coding synonymous_variant LOW 744A>G Glu248Glu
M0048257 ENHDLEAH_00007 6186 3 Skin 0.21 protein_coding synonymous_variant LOW 1197C>G Arg399Arg
M0048258 ENHDLEAH_00007 6252 3 Skin 0.21 protein_coding synonymous_variant LOW 1263T>C Phe421Phe
M0048259 ENHDLEAH_00007 6552 3 Skin 0.21 protein_coding synonymous_variant LOW 1563C>T Ala521Ala
M0048260 ENHDLEAH_00007 7059 4 Skin 0.29 protein_coding synonymous_variant LOW 2070C>A Gly690Gly
M0048261 ENHDLEAH_00007 7075 4 Skin 0.29 protein_coding missense_variant MODERATE 2086A>T Met696Leu
M0048262 ENHDLEAH_00007 7161 3 Skin 0.21 protein_coding synonymous_variant LOW 2172G>T Ala724Ala
M0048263 ENHDLEAH_00010 8950 3 Skin 0.21 protein_coding synonymous_variant LOW 726G>A Glu242Glu
M0048264 ENHDLEAH_00010 8956 3 Skin 0.21 protein_coding synonymous_variant LOW 720G>A Gly240Gly
M0048265 ENHDLEAH_00010 8959 3 Skin 0.21 protein_coding synonymous_variant LOW 717T>A Arg239Arg
M0048266 ENHDLEAH_00010 8976 3 Skin 0.21 protein_coding synonymous_variant LOW 700C>T Leu234Leu
M0048267 ENHDLEAH_00010 8977 3 Skin 0.21 protein_coding synonymous_variant LOW 699T>C Phe233Phe
M0048268 ENHDLEAH_00010 9015 3 Skin 0.21 protein_coding missense_variant MODERATE 661C>A Leu221Ile
M0048269 ENHDLEAH_00010 9043 3 Skin 0.21 protein_coding synonymous_variant LOW 633A>G Arg211Arg
M0048270 ENHDLEAH_00010 9048 3 Skin 0.21 protein_coding synonymous_variant LOW 628T>C Leu210Leu
M0048271 ENHDLEAH_00010 9079 3 Skin 0.21 protein_coding synonymous_variant LOW 597T>C Val199Val
M0048272 ENHDLEAH_00010 9085 3 Skin 0.21 protein_coding synonymous_variant LOW 591T>C Thr197Thr
M0048273 ENHDLEAH_00010 9133 3 Skin 0.21 protein_coding synonymous_variant LOW 543T>C Asp181Asp
M0048274 ENHDLEAH_00010 10503 3 Skin 0.21 protein_coding upstream_gene_variant MODIFIER -828A>G None
M0048275 ENHDLEAH_00010 10506 3 Skin 0.21 protein_coding upstream_gene_variant MODIFIER -831G>A None
M0048276 ENHDLEAH_00010 10511 3 Skin 0.21 protein_coding upstream_gene_variant MODIFIER -836A>G None
M0048277 ENHDLEAH_00012 10650 4 Skin 0.29 protein_coding missense_variant MODERATE 16A>C Ile6Leu
M0048278 ENHDLEAH_00012 10667 4 Skin 0.29 protein_coding synonymous_variant LOW 33C>G Ala11Ala
M0048279 ENHDLEAH_00012 10671 4 Skin 0.29 protein_coding missense_variant MODERATE 37G>A Val13Ile
M0048280 ENHDLEAH_00015 14683 3 Skin 0.21 protein_coding synonymous_variant LOW 49T>C Leu17Leu






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group
ENHDLEAH_00015 Cadmium (Cd), Zinc (Zn), Cobalt (Co) 77.7 0 1 1055 0.9944 0.9934 experiment
ENHDLEAH_00015 Cadmium (Cd), Zinc (Zn), Cobalt (Co) 78.5 0 1 1050 0.9896 0.9869 prediction






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
ENHDLEAH_00015 2.A.6.1.2 77.7 0 1 1055 0.9934 0.9868 2 Electrochemical Potential-driven Transporters 2.A Porters (uniporters, symporters, antiporters) 2.A.6 The Resistance-Nodulation-Cell Division (RND) Superfamily