Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C21
  Reference Plasmid   1111525849861219_bin.29__k141_116707
  Reference Plasmid Size   17063
  Reference Plasmid GC Content   0.42
  Reference Plasmid Mobility Type   non-mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0053229 HFHMHMCK_00006 3981 3 Skin 0.60 protein_coding missense_variant MODERATE 130A>G Lys44Glu
M0053230 HFHMHMCK_00006 4083 3 Skin 0.60 protein_coding missense_variant MODERATE 28C>A Gln10Lys
M0131158 HFHMHMCK_00002 613 4 Oral 0.80 protein_coding upstream_gene_variant MODIFIER -119T>C None
M0131159 HFHMHMCK_00002 632 4 Oral 0.80 protein_coding upstream_gene_variant MODIFIER -100C>A None
M0131160 HFHMHMCK_00002 851 5 Oral 1.00 protein_coding synonymous_variant LOW 120A>G Gly40Gly
M0131161 HFHMHMCK_00002 1222 5 Oral 1.00 protein_coding missense_variant MODERATE 491T>C Ile164Thr
M0131162 HFHMHMCK_00002 1265 5 Oral 1.00 protein_coding synonymous_variant LOW 534T>C Ala178Ala
M0131163 HFHMHMCK_00003 1363 5 Oral 1.00 protein_coding synonymous_variant LOW 33T>G Ala11Ala
M0131164 HFHMHMCK_00003 1760 5 Oral 1.00 protein_coding synonymous_variant LOW 430T>C Leu144Leu
M0131165 HFHMHMCK_00003 1922 5 Oral 1.00 protein_coding synonymous_variant LOW 592T>C Leu198Leu
M0131166 HFHMHMCK_00003 2047 5 Oral 1.00 protein_coding synonymous_variant LOW 717A>G Arg239Arg
M0131167 HFHMHMCK_00004 2363 5 Oral 1.00 protein_coding missense_variant MODERATE 263G>A Ser88Asn
M0131168 HFHMHMCK_00004 2416 5 Oral 1.00 protein_coding missense_variant MODERATE 316T>G Phe106Val
M0131169 HFHMHMCK_00004 2685 5 Oral 1.00 protein_coding missense_variant MODERATE 585T>G Asp195Glu
M0131170 HFHMHMCK_00005 2987 4 Oral 0.80 protein_coding missense_variant MODERATE 64A>G Ile22Val
M0131171 HFHMHMCK_00005 2998 4 Oral 0.80 protein_coding synonymous_variant LOW 75G>A Lys25Lys
M0131172 HFHMHMCK_00005 3703 5 Oral 1.00 protein_coding synonymous_variant LOW 780T>C Cys260Cys
M0131173 HFHMHMCK_00009 3786 5 Oral 1.00 protein_coding upstream_gene_variant MODIFIER -1651T>C None
M0131174 HFHMHMCK_00006 3981 3 Oral 0.60 protein_coding missense_variant MODERATE 130A>G Lys44Glu
M0131175 HFHMHMCK_00006 4083 3 Oral 0.60 protein_coding missense_variant MODERATE 28C>A Gln10Lys
M0131176 HFHMHMCK_00004 2927 3 Oral 0.60 protein_coding missense_variant MODERATE 827C>T Ala276Val






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
HFHMHMCK_00011 AGL64020.2|AA10 77.8 6.54e-194 1 345 1 1





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term