Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C2714
  Reference Plasmid   1111525849861727_bin.16__k141_103548
  Reference Plasmid Size   8805
  Reference Plasmid GC Content   0.40
  Reference Plasmid Mobility Type   non-mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0131234 MDEMHBMJ_00003 2244 5 Oral 0.83 protein_coding synonymous_variant LOW 924T>C Gly308Gly
M0131235 MDEMHBMJ_00003 2250 3 Oral 0.50 protein_coding synonymous_variant LOW 918A>T Thr306Thr
M0131236 MDEMHBMJ_00003 2298 3 Oral 0.50 protein_coding synonymous_variant LOW 870T>A Gly290Gly
M0131237 MDEMHBMJ_00003 2336 5 Oral 0.83 protein_coding missense_variant MODERATE 832C>T Pro278Ser
M0131238 MDEMHBMJ_00003 2340 5 Oral 0.83 protein_coding synonymous_variant LOW 828T>C Thr276Thr
M0131239 MDEMHBMJ_00003 2341 5 Oral 0.83 protein_coding missense_variant MODERATE 827C>G Thr276Ser
M0131240 MDEMHBMJ_00003 2406 3 Oral 0.50 protein_coding synonymous_variant LOW 762C>T Ala254Ala
M0131241 MDEMHBMJ_00003 2438 5 Oral 0.83 protein_coding missense_variant MODERATE 730C>A Leu244Ile
M0131242 MDEMHBMJ_00003 2452 5 Oral 0.83 protein_coding missense_variant MODERATE 716G>A Arg239Lys
M0131243 MDEMHBMJ_00003 2592 5 Oral 0.83 protein_coding synonymous_variant LOW 576A>G Leu192Leu
M0131244 MDEMHBMJ_00003 2613 5 Oral 0.83 protein_coding synonymous_variant LOW 555C>T Thr185Thr
M0131245 MDEMHBMJ_00003 2616 5 Oral 0.83 protein_coding synonymous_variant LOW 552T>C Ile184Ile
M0131246 MDEMHBMJ_00003 2661 3 Oral 0.50 protein_coding synonymous_variant LOW 507T>C Asp169Asp
M0131247 MDEMHBMJ_00003 2664 5 Oral 0.83 protein_coding synonymous_variant LOW 504A>C Gly168Gly
M0131248 MDEMHBMJ_00003 2782 5 Oral 0.83 protein_coding missense_variant MODERATE 386T>A Phe129Tyr
M0131249 MDEMHBMJ_00003 2856 3 Oral 0.50 protein_coding synonymous_variant LOW 312T>C Thr104Thr
M0131250 MDEMHBMJ_00003 2862 4 Oral 0.67 protein_coding synonymous_variant LOW 306T>C Asn102Asn
M0131251 MDEMHBMJ_00003 2958 3 Oral 0.50 protein_coding synonymous_variant LOW 210A>G Ala70Ala
M0131252 MDEMHBMJ_00003 2960 5 Oral 0.83 protein_coding missense_variant MODERATE 208G>T Ala70Ser
M0131253 MDEMHBMJ_00003 3033 4 Oral 0.67 protein_coding synonymous_variant LOW 135C>T Gly45Gly
M0131254 MDEMHBMJ_00003 3072 5 Oral 0.83 protein_coding synonymous_variant LOW 96G>A Arg32Arg
M0131255 MDEMHBMJ_00004 3310 5 Oral 0.83 protein_coding synonymous_variant LOW 2628T>C His876His
M0131256 MDEMHBMJ_00004 3316 5 Oral 0.83 protein_coding synonymous_variant LOW 2622T>C Arg874Arg
M0131257 MDEMHBMJ_00004 3379 4 Oral 0.67 protein_coding synonymous_variant LOW 2559A>G Leu853Leu
M0131258 MDEMHBMJ_00004 3719 5 Oral 0.83 protein_coding missense_variant MODERATE 2219C>G Ser740Cys
M0131259 MDEMHBMJ_00004 3775 5 Oral 0.83 protein_coding synonymous_variant LOW 2163A>G Leu721Leu
M0131260 MDEMHBMJ_00004 4129 4 Oral 0.67 protein_coding synonymous_variant LOW 1809G>A Glu603Glu
M0131261 MDEMHBMJ_00004 4213 4 Oral 0.67 protein_coding synonymous_variant LOW 1725A>T Ile575Ile
M0131262 MDEMHBMJ_00004 4228 3 Oral 0.50 protein_coding synonymous_variant LOW 1710G>A Leu570Leu
M0131263 MDEMHBMJ_00004 4357 3 Oral 0.50 protein_coding synonymous_variant LOW 1581T>A Arg527Arg
M0131264 MDEMHBMJ_00004 4558 4 Oral 0.67 protein_coding synonymous_variant LOW 1380A>G Lys460Lys
M0131265 MDEMHBMJ_00004 4567 4 Oral 0.67 protein_coding synonymous_variant LOW 1371T>C Ala457Ala
M0131266 MDEMHBMJ_00004 4639 3 Oral 0.50 protein_coding synonymous_variant LOW 1299T>C Tyr433Tyr
M0131267 MDEMHBMJ_00004 4649 5 Oral 0.83 protein_coding missense_variant MODERATE 1289A>G Asn430Ser
M0131268 MDEMHBMJ_00004 4668 5 Oral 0.83 protein_coding missense_variant MODERATE 1270T>C Ser424Pro
M0131269 MDEMHBMJ_00004 4680 5 Oral 0.83 protein_coding missense_variant MODERATE 1258T>A Cys420Ser
M0131270 MDEMHBMJ_00004 4699 3 Oral 0.50 protein_coding synonymous_variant LOW 1239G>A Glu413Glu
M0131271 MDEMHBMJ_00004 4837 5 Oral 0.83 protein_coding synonymous_variant LOW 1101G>A Lys367Lys
M0131272 MDEMHBMJ_00004 4839 5 Oral 0.83 protein_coding missense_variant MODERATE 1099A>C Lys367Gln
M0131273 MDEMHBMJ_00004 4860 5 Oral 0.83 protein_coding missense_variant MODERATE 1078A>G Lys360Glu
M0131274 MDEMHBMJ_00004 4912 4 Oral 0.67 protein_coding synonymous_variant LOW 1026G>A Glu342Glu
M0131275 MDEMHBMJ_00004 4924 5 Oral 0.83 protein_coding synonymous_variant LOW 1014A>G Lys338Lys
M0131276 MDEMHBMJ_00004 5014 5 Oral 0.83 protein_coding synonymous_variant LOW 924T>C Cys308Cys
M0131277 MDEMHBMJ_00004 5128 5 Oral 0.83 protein_coding synonymous_variant LOW 810T>C Asp270Asp
M0131278 MDEMHBMJ_00004 5140 3 Oral 0.50 protein_coding synonymous_variant LOW 798C>A Gly266Gly
M0131279 MDEMHBMJ_00004 5248 5 Oral 0.83 protein_coding synonymous_variant LOW 690T>A Thr230Thr
M0131280 MDEMHBMJ_00004 5329 5 Oral 0.83 protein_coding synonymous_variant LOW 609T>C Asn203Asn
M0131281 MDEMHBMJ_00004 5413 5 Oral 0.83 protein_coding synonymous_variant LOW 525A>G Glu175Glu
M0131282 MDEMHBMJ_00004 5456 5 Oral 0.83 protein_coding missense_variant MODERATE 482C>T Ala161Val
M0131283 MDEMHBMJ_00004 5605 5 Oral 0.83 protein_coding synonymous_variant LOW 333C>T Tyr111Tyr
M0131284 MDEMHBMJ_00004 5713 5 Oral 0.83 protein_coding synonymous_variant LOW 225T>C Val75Val
M0131285 MDEMHBMJ_00004 5815 3 Oral 0.50 protein_coding synonymous_variant LOW 123T>C Asn41Asn
M0131286 MDEMHBMJ_00001 5972 5 Oral 0.83 protein_coding upstream_gene_variant MODIFIER -4616G>C None
M0131287 MDEMHBMJ_00001 5983 3 Oral 0.50 protein_coding upstream_gene_variant MODIFIER -4627C>T None
M0131288 MDEMHBMJ_00001 6042 5 Oral 0.83 protein_coding upstream_gene_variant MODIFIER -4686G>A None
M0131289 MDEMHBMJ_00005 6223 5 Oral 0.83 protein_coding synonymous_variant LOW 111A>T Ser37Ser
M0131290 MDEMHBMJ_00005 6301 5 Oral 0.83 protein_coding synonymous_variant LOW 189T>A Thr63Thr
M0131291 MDEMHBMJ_00005 6697 5 Oral 0.83 protein_coding synonymous_variant LOW 585G>A Gly195Gly
M0131292 MDEMHBMJ_00005 6741 4 Oral 0.67 protein_coding missense_variant MODERATE 629G>A Cys210Tyr
M0131293 MDEMHBMJ_00005 6772 4 Oral 0.67 protein_coding synonymous_variant LOW 660T>C Ala220Ala
M0131294 MDEMHBMJ_00005 6782 4 Oral 0.67 protein_coding missense_variant MODERATE 670A>G Ile224Val
M0131295 MDEMHBMJ_00005 6855 4 Oral 0.67 protein_coding missense_variant MODERATE 743T>C Met248Thr
M0131296 MDEMHBMJ_00005 6914 4 Oral 0.67 protein_coding missense_variant MODERATE 802A>C Lys268Gln
M0131297 MDEMHBMJ_00005 6936 4 Oral 0.67 protein_coding missense_variant MODERATE 824G>A Arg275Gln
M0131298 MDEMHBMJ_00005 6961 5 Oral 0.83 protein_coding synonymous_variant LOW 849A>T Ala283Ala
M0131299 MDEMHBMJ_00003 1866 4 Oral 0.67 protein_coding synonymous_variant LOW 1302T>C Cys434Cys
M0131300 MDEMHBMJ_00003 1884 4 Oral 0.67 protein_coding synonymous_variant LOW 1284A>T Thr428Thr
M0131301 MDEMHBMJ_00003 1893 4 Oral 0.67 protein_coding synonymous_variant LOW 1275A>G Leu425Leu
M0131302 MDEMHBMJ_00003 1901 4 Oral 0.67 protein_coding missense_variant MODERATE 1267A>G Thr423Ala
M0131303 MDEMHBMJ_00003 1923 4 Oral 0.67 protein_coding synonymous_variant LOW 1245T>C Phe415Phe
M0131304 MDEMHBMJ_00003 1977 4 Oral 0.67 protein_coding synonymous_variant LOW 1191T>C Cys397Cys
M0131305 MDEMHBMJ_00003 1993 4 Oral 0.67 protein_coding missense_variant MODERATE 1175T>C Leu392Ser
M0131306 MDEMHBMJ_00003 2127 4 Oral 0.67 protein_coding synonymous_variant LOW 1041A>T Pro347Pro
M0131307 MDEMHBMJ_00003 2586 3 Oral 0.50 protein_coding synonymous_variant LOW 582G>A Gly194Gly
M0131308 MDEMHBMJ_00003 2754 4 Oral 0.67 protein_coding synonymous_variant LOW 414C>T Leu138Leu
M0131309 MDEMHBMJ_00004 3967 3 Oral 0.50 protein_coding synonymous_variant LOW 1971C>T Ser657Ser
M0131310 MDEMHBMJ_00004 4138 3 Oral 0.50 protein_coding synonymous_variant LOW 1800G>A Val600Val
M0131311 MDEMHBMJ_00004 4405 4 Oral 0.67 protein_coding synonymous_variant LOW 1533C>T Tyr511Tyr
M0131312 MDEMHBMJ_00004 4657 4 Oral 0.67 protein_coding synonymous_variant LOW 1281T>C Asn427Asn
M0131313 MDEMHBMJ_00004 4660 4 Oral 0.67 protein_coding synonymous_variant LOW 1278C>T Ser426Ser
M0131314 MDEMHBMJ_00004 4661 4 Oral 0.67 protein_coding missense_variant MODERATE 1277G>C Ser426Thr
M0131315 MDEMHBMJ_00004 4662 4 Oral 0.67 protein_coding missense_variant MODERATE 1276A>T Ser426Cys
M0131316 MDEMHBMJ_00004 4800 3 Oral 0.50 protein_coding missense_variant MODERATE 1138G>T Ala380Ser
M0131317 MDEMHBMJ_00004 4827 3 Oral 0.50 protein_coding missense_variant MODERATE 1111G>A Ala371Thr
M0131318 MDEMHBMJ_00004 4828 3 Oral 0.50 protein_coding synonymous_variant LOW 1110A>T Gly370Gly
M0131319 MDEMHBMJ_00004 4990 3 Oral 0.50 protein_coding synonymous_variant LOW 948G>A Pro316Pro
M0131320 MDEMHBMJ_00004 5801 4 Oral 0.67 protein_coding missense_variant MODERATE 137G>A Arg46His
M0131321 MDEMHBMJ_00005 6475 3 Oral 0.50 protein_coding synonymous_variant LOW 363C>T Phe121Phe
M0131322 MDEMHBMJ_00005 6835 3 Oral 0.50 protein_coding synonymous_variant LOW 723T>C Ala241Ala
M0131323 MDEMHBMJ_00006 7248 3 Oral 0.50 protein_coding synonymous_variant LOW 252T>C Phe84Phe
M0131324 MDEMHBMJ_00006 7275 3 Oral 0.50 protein_coding synonymous_variant LOW 279T>C Arg93Arg
M0131325 MDEMHBMJ_00006 7548 3 Oral 0.50 protein_coding synonymous_variant LOW 552A>T Ala184Ala
M0131326 MDEMHBMJ_00003 7780 3 Oral 0.50 protein_coding upstream_gene_variant MODIFIER -4613G>A None
M0131327 MDEMHBMJ_00007 7790 3 Oral 0.50 protein_coding start_lost HIGH 3G>A Met1?
M0131328 MDEMHBMJ_00007 7840 3 Oral 0.50 protein_coding missense_variant MODERATE 53T>C Val18Ala
M0131329 MDEMHBMJ_00007 7909 3 Oral 0.50 protein_coding missense_variant MODERATE 122G>A Ser41Asn
M0131330 MDEMHBMJ_00008 8008 3 Oral 0.50 protein_coding synonymous_variant LOW 657G>A Val219Val
M0131331 MDEMHBMJ_00008 8037 3 Oral 0.50 protein_coding synonymous_variant LOW 628C>T Leu210Leu
M0131332 MDEMHBMJ_00008 8074 3 Oral 0.50 protein_coding synonymous_variant LOW 591C>T Cys197Cys
M0131333 MDEMHBMJ_00008 8249 3 Oral 0.50 protein_coding missense_variant MODERATE 416G>A Arg139Lys
M0131334 MDEMHBMJ_00008 8443 3 Oral 0.50 protein_coding synonymous_variant LOW 222T>C Ala74Ala
M0131335 MDEMHBMJ_00008 8566 3 Oral 0.50 protein_coding synonymous_variant LOW 99A>G Ser33Ser
M0131336 MDEMHBMJ_00008 8620 3 Oral 0.50 protein_coding synonymous_variant LOW 45G>C Gly15Gly
M0131337 MDEMHBMJ_00008 8635 3 Oral 0.50 protein_coding synonymous_variant LOW 30C>T Ile10Ile
M0131338 MDEMHBMJ_00004 8666 3 Oral 0.50 protein_coding upstream_gene_variant MODIFIER -2729C>A None
M0131339 MDEMHBMJ_00004 8668 3 Oral 0.50 protein_coding upstream_gene_variant MODIFIER -2731C>A None
M0131340 MDEMHBMJ_00004 8669 3 Oral 0.50 protein_coding upstream_gene_variant MODIFIER -2732T>C None
M0131341 MDEMHBMJ_00004 8694 3 Oral 0.50 protein_coding upstream_gene_variant MODIFIER -2757C>T None
M0131342 MDEMHBMJ_00004 8702 3 Oral 0.50 protein_coding upstream_gene_variant MODIFIER -2765T>C None
M0131343 MDEMHBMJ_00004 8707 3 Oral 0.50 protein_coding upstream_gene_variant MODIFIER -2770G>A None
M0131344 MDEMHBMJ_00003 2109 3 Oral 0.50 protein_coding synonymous_variant LOW 1059A>T Ala353Ala
M0131345 MDEMHBMJ_00003 2167 3 Oral 0.50 protein_coding missense_variant MODERATE 1001C>A Ala334Glu
M0131346 MDEMHBMJ_00003 2169 3 Oral 0.50 protein_coding synonymous_variant LOW 999G>A Thr333Thr






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
MDEMHBMJ_00004 EFC70588.2|CBM20|GH77 86.9 0 1 901 1 1





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term