Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C2723
  Reference Plasmid   1111525849861727_bin.16__k141_155955
  Reference Plasmid Size   8057
  Reference Plasmid GC Content   0.43
  Reference Plasmid Mobility Type   non-mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0131871 MKMHILHB_00003 980 4 Oral 0.67 protein_coding upstream_gene_variant MODIFIER -7C>T None
M0131872 MKMHILHB_00003 1071 6 Oral 1.00 protein_coding missense_variant MODERATE 85A>G Ile29Val
M0131873 MKMHILHB_00003 1133 6 Oral 1.00 protein_coding synonymous_variant LOW 147G>T Ala49Ala
M0131874 MKMHILHB_00003 1139 3 Oral 0.50 protein_coding synonymous_variant LOW 153T>C Pro51Pro
M0131875 MKMHILHB_00003 1380 5 Oral 0.83 protein_coding synonymous_variant LOW 394T>C Leu132Leu
M0131876 MKMHILHB_00003 1382 5 Oral 0.83 protein_coding synonymous_variant LOW 396A>G Leu132Leu
M0131877 MKMHILHB_00003 1724 6 Oral 1.00 protein_coding synonymous_variant LOW 738T>G Ala246Ala
M0131878 MKMHILHB_00003 1802 6 Oral 1.00 protein_coding synonymous_variant LOW 816T>C Gly272Gly
M0131879 MKMHILHB_00003 2183 4 Oral 0.67 protein_coding synonymous_variant LOW 1197A>G Ala399Ala
M0131880 MKMHILHB_00003 2292 4 Oral 0.67 protein_coding synonymous_variant LOW 1306T>C Leu436Leu
M0131881 MKMHILHB_00003 2343 6 Oral 1.00 protein_coding stop_lost&splice_region_variant HIGH 1357T>G Ter453Gluext*?
M0131882 MKMHILHB_00004 2456 4 Oral 0.67 protein_coding synonymous_variant LOW 87G>T Ala29Ala
M0131883 MKMHILHB_00004 2603 6 Oral 1.00 protein_coding synonymous_variant LOW 234C>T Ala78Ala
M0131884 MKMHILHB_00004 2610 5 Oral 0.83 protein_coding missense_variant MODERATE 241C>A Leu81Ile
M0131885 MKMHILHB_00004 2621 4 Oral 0.67 protein_coding synonymous_variant LOW 252A>G Lys84Lys
M0131886 MKMHILHB_00004 2881 6 Oral 1.00 protein_coding missense_variant MODERATE 512C>T Ala171Val
M0131887 MKMHILHB_00004 2883 6 Oral 1.00 protein_coding missense_variant MODERATE 514A>G Ile172Val
M0131888 MKMHILHB_00004 2884 6 Oral 1.00 protein_coding missense_variant MODERATE 515T>C Ile172Thr
M0131889 MKMHILHB_00004 2930 6 Oral 1.00 protein_coding synonymous_variant LOW 561A>G Glu187Glu
M0131890 MKMHILHB_00004 3191 6 Oral 1.00 protein_coding synonymous_variant LOW 822T>C Thr274Thr
M0131891 MKMHILHB_00004 3236 5 Oral 0.83 protein_coding synonymous_variant LOW 867C>T Asp289Asp
M0131892 MKMHILHB_00004 3317 3 Oral 0.50 protein_coding synonymous_variant LOW 948A>G Lys316Lys
M0131893 MKMHILHB_00004 3338 3 Oral 0.50 protein_coding synonymous_variant LOW 969T>C Asp323Asp
M0131894 MKMHILHB_00004 3842 6 Oral 1.00 protein_coding synonymous_variant LOW 1473C>T Gly491Gly
M0131895 MKMHILHB_00004 3905 6 Oral 1.00 protein_coding synonymous_variant LOW 1536T>C Asp512Asp
M0131896 MKMHILHB_00004 3911 6 Oral 1.00 protein_coding synonymous_variant LOW 1542T>A Val514Val
M0131897 MKMHILHB_00004 3920 6 Oral 1.00 protein_coding synonymous_variant LOW 1551T>A Val517Val
M0131898 MKMHILHB_00004 3926 6 Oral 1.00 protein_coding synonymous_variant LOW 1557A>G Val519Val
M0131899 MKMHILHB_00004 3947 6 Oral 1.00 protein_coding synonymous_variant LOW 1578A>G Thr526Thr
M0131900 MKMHILHB_00004 4181 6 Oral 1.00 protein_coding synonymous_variant LOW 1812A>G Gly604Gly
M0131901 MKMHILHB_00004 4194 6 Oral 1.00 protein_coding missense_variant MODERATE 1825C>T Pro609Ser
M0131902 MKMHILHB_00004 4388 6 Oral 1.00 protein_coding synonymous_variant LOW 2019T>C Ile673Ile
M0131903 MKMHILHB_00004 4628 3 Oral 0.50 protein_coding synonymous_variant LOW 2259G>C Ala753Ala
M0131904 MKMHILHB_00005 4656 5 Oral 0.83 protein_coding upstream_gene_variant MODIFIER -138T>G None
M0131905 MKMHILHB_00005 4663 6 Oral 1.00 protein_coding upstream_gene_variant MODIFIER -131T>G None
M0131906 MKMHILHB_00005 4664 6 Oral 1.00 protein_coding upstream_gene_variant MODIFIER -130T>C None
M0131907 MKMHILHB_00005 4665 4 Oral 0.67 protein_coding upstream_gene_variant MODIFIER -129G>A None
M0131908 MKMHILHB_00005 4685 6 Oral 1.00 protein_coding upstream_gene_variant MODIFIER -109T>C None
M0131909 MKMHILHB_00005 4734 3 Oral 0.50 protein_coding upstream_gene_variant MODIFIER -60T>C None
M0131910 MKMHILHB_00005 5003 5 Oral 0.83 protein_coding synonymous_variant LOW 210A>C Leu70Leu
M0131911 MKMHILHB_00005 5077 5 Oral 0.83 protein_coding missense_variant MODERATE 284A>T Lys95Met
M0131912 MKMHILHB_00005 5093 3 Oral 0.50 protein_coding synonymous_variant LOW 300T>C Gly100Gly
M0131913 MKMHILHB_00005 5108 3 Oral 0.50 protein_coding synonymous_variant LOW 315G>A Thr105Thr
M0131914 MKMHILHB_00005 5136 5 Oral 0.83 protein_coding missense_variant MODERATE 343A>G Thr115Ala
M0131915 MKMHILHB_00005 5298 3 Oral 0.50 protein_coding missense_variant MODERATE 505G>T Ala169Ser
M0131916 MKMHILHB_00005 5396 5 Oral 0.83 protein_coding synonymous_variant LOW 603C>T Ile201Ile
M0131917 MKMHILHB_00005 5730 4 Oral 0.67 protein_coding missense_variant MODERATE 937C>A Gln313Lys
M0131918 MKMHILHB_00005 5734 5 Oral 0.83 protein_coding missense_variant MODERATE 941G>A Arg314Lys
M0131919 MKMHILHB_00005 5774 5 Oral 0.83 protein_coding synonymous_variant LOW 981G>A Gln327Gln
M0131920 MKMHILHB_00005 5987 4 Oral 0.67 protein_coding synonymous_variant LOW 1194T>C Asp398Asp
M0131921 MKMHILHB_00005 6009 5 Oral 0.83 protein_coding missense_variant MODERATE 1216G>A Gly406Ser
M0131922 MKMHILHB_00005 6018 5 Oral 0.83 protein_coding missense_variant MODERATE 1225A>G Thr409Ala
M0131923 MKMHILHB_00006 6295 5 Oral 0.83 protein_coding synonymous_variant LOW 198G>A Gly66Gly
M0131924 MKMHILHB_00006 6487 5 Oral 0.83 protein_coding synonymous_variant LOW 390A>C Arg130Arg
M0131925 MKMHILHB_00006 6508 4 Oral 0.67 protein_coding synonymous_variant LOW 411A>G Ala137Ala
M0131926 MKMHILHB_00006 6820 5 Oral 0.83 protein_coding synonymous_variant LOW 723C>T Ser241Ser
M0131927 MKMHILHB_00006 7100 3 Oral 0.50 protein_coding synonymous_variant LOW 1003C>T Leu335Leu
M0131928 MKMHILHB_00006 7102 3 Oral 0.50 protein_coding synonymous_variant LOW 1005G>A Leu335Leu
M0131929 MKMHILHB_00006 7171 5 Oral 0.83 protein_coding synonymous_variant LOW 1074T>G Val358Val
M0131930 MKMHILHB_00007 7615 4 Oral 0.67 protein_coding synonymous_variant LOW 159T>C Asn53Asn
M0131931 MKMHILHB_00004 7819 3 Oral 0.50 protein_coding downstream_gene_variant MODIFIER *3164C>T None
M0131932 MKMHILHB_00004 7821 5 Oral 0.83 protein_coding downstream_gene_variant MODIFIER *3166A>G None
M0131933 MKMHILHB_00004 7894 5 Oral 0.83 protein_coding downstream_gene_variant MODIFIER *3239T>C None
M0131934 MKMHILHB_00001 352 3 Oral 0.50 protein_coding missense_variant MODERATE 179C>T Thr60Ile
M0131935 MKMHILHB_00002 760 4 Oral 0.67 protein_coding missense_variant MODERATE 389A>G Gln130Arg
M0131936 MKMHILHB_00002 785 5 Oral 0.83 protein_coding synonymous_variant LOW 414G>A Pro138Pro
M0131937 MKMHILHB_00003 1493 5 Oral 0.83 protein_coding synonymous_variant LOW 507C>T Gly169Gly
M0131938 MKMHILHB_00003 1591 3 Oral 0.50 protein_coding missense_variant MODERATE 605T>A Phe202Tyr
M0131939 MKMHILHB_00003 1719 5 Oral 0.83 protein_coding missense_variant MODERATE 733G>A Glu245Lys
M0131940 MKMHILHB_00003 1836 5 Oral 0.83 protein_coding missense_variant MODERATE 850T>A Ser284Thr
M0131941 MKMHILHB_00003 1838 5 Oral 0.83 protein_coding synonymous_variant LOW 852G>A Ser284Ser
M0131942 MKMHILHB_00004 2568 3 Oral 0.50 protein_coding missense_variant MODERATE 199G>A Val67Ile
M0131943 MKMHILHB_00004 2687 5 Oral 0.83 protein_coding synonymous_variant LOW 318G>A Ala106Ala
M0131944 MKMHILHB_00004 2799 5 Oral 0.83 protein_coding missense_variant MODERATE 430T>C Phe144Leu
M0131945 MKMHILHB_00004 2951 4 Oral 0.67 protein_coding synonymous_variant LOW 582G>A Glu194Glu
M0131946 MKMHILHB_00004 3020 5 Oral 0.83 protein_coding synonymous_variant LOW 651C>A Arg217Arg
M0131947 MKMHILHB_00004 3032 4 Oral 0.67 protein_coding synonymous_variant LOW 663C>T Arg221Arg
M0131948 MKMHILHB_00004 3257 5 Oral 0.83 protein_coding synonymous_variant LOW 888C>A Ile296Ile
M0131949 MKMHILHB_00004 3273 5 Oral 0.83 protein_coding synonymous_variant LOW 904C>T Leu302Leu
M0131950 MKMHILHB_00004 3806 5 Oral 0.83 protein_coding synonymous_variant LOW 1437A>G Pro479Pro
M0131951 MKMHILHB_00004 3848 3 Oral 0.50 protein_coding synonymous_variant LOW 1479T>C Thr493Thr
M0131952 MKMHILHB_00004 4049 3 Oral 0.50 protein_coding synonymous_variant LOW 1680G>A Leu560Leu
M0131953 MKMHILHB_00004 4226 3 Oral 0.50 protein_coding synonymous_variant LOW 1857G>A Gln619Gln
M0131954 MKMHILHB_00004 4247 4 Oral 0.67 protein_coding synonymous_variant LOW 1878T>C Asp626Asp
M0131955 MKMHILHB_00005 4750 3 Oral 0.50 protein_coding upstream_gene_variant MODIFIER -44T>A None
M0131956 MKMHILHB_00005 4820 3 Oral 0.50 protein_coding missense_variant MODERATE 27A>G Ile9Met
M0131957 MKMHILHB_00005 4849 4 Oral 0.67 protein_coding missense_variant MODERATE 56T>C Val19Ala
M0131958 MKMHILHB_00005 4989 4 Oral 0.67 protein_coding missense_variant MODERATE 196T>C Ser66Pro
M0131959 MKMHILHB_00005 5038 3 Oral 0.50 protein_coding missense_variant MODERATE 245T>A Val82Glu
M0131960 MKMHILHB_00005 5039 3 Oral 0.50 protein_coding synonymous_variant LOW 246G>A Val82Val
M0131961 MKMHILHB_00005 5084 4 Oral 0.67 protein_coding synonymous_variant LOW 291C>T Pro97Pro
M0131962 MKMHILHB_00005 5546 3 Oral 0.50 protein_coding synonymous_variant LOW 753G>A Ala251Ala
M0131963 MKMHILHB_00005 5567 3 Oral 0.50 protein_coding synonymous_variant LOW 774G>A Ala258Ala
M0131964 MKMHILHB_00005 5888 4 Oral 0.67 protein_coding synonymous_variant LOW 1095C>T Ile365Ile
M0131965 MKMHILHB_00006 6338 4 Oral 0.67 protein_coding missense_variant MODERATE 241A>T Thr81Ser
M0131966 MKMHILHB_00006 6349 4 Oral 0.67 protein_coding synonymous_variant LOW 252T>C Ser84Ser
M0131967 MKMHILHB_00006 6350 4 Oral 0.67 protein_coding missense_variant MODERATE 253A>C Lys85Gln
M0131968 MKMHILHB_00006 6371 4 Oral 0.67 protein_coding missense_variant MODERATE 274G>T Ala92Ser
M0131969 MKMHILHB_00006 6433 3 Oral 0.50 protein_coding synonymous_variant LOW 336G>A Thr112Thr
M0131970 MKMHILHB_00006 6545 3 Oral 0.50 protein_coding missense_variant MODERATE 448G>T Ala150Ser
M0131971 MKMHILHB_00006 6619 4 Oral 0.67 protein_coding synonymous_variant LOW 522A>G Gln174Gln
M0131972 MKMHILHB_00006 6625 4 Oral 0.67 protein_coding synonymous_variant LOW 528A>T Leu176Leu
M0131973 MKMHILHB_00006 6940 3 Oral 0.50 protein_coding synonymous_variant LOW 843G>A Thr281Thr
M0131974 MKMHILHB_00006 6949 3 Oral 0.50 protein_coding synonymous_variant LOW 852A>T Gly284Gly
M0131975 MKMHILHB_00006 7055 3 Oral 0.50 protein_coding missense_variant MODERATE 958G>A Val320Ile
M0131976 MKMHILHB_00006 7159 3 Oral 0.50 protein_coding synonymous_variant LOW 1062G>A Lys354Lys
M0131977 MKMHILHB_00007 7399 3 Oral 0.50 protein_coding upstream_gene_variant MODIFIER -58A>T None
M0131978 MKMHILHB_00007 7402 3 Oral 0.50 protein_coding upstream_gene_variant MODIFIER -55T>C None
M0131979 MKMHILHB_00007 7403 3 Oral 0.50 protein_coding upstream_gene_variant MODIFIER -54T>A None
M0131980 MKMHILHB_00007 7675 3 Oral 0.50 protein_coding synonymous_variant LOW 219A>G Glu73Glu
M0131981 MKMHILHB_00007 7678 4 Oral 0.67 protein_coding missense_variant MODERATE 222G>T Lys74Asn
M0131982 MKMHILHB_00002 572 4 Oral 0.67 protein_coding synonymous_variant LOW 201C>T His67His
M0131983 MKMHILHB_00003 914 3 Oral 0.50 protein_coding upstream_gene_variant MODIFIER -73T>C None
M0131984 MKMHILHB_00003 965 3 Oral 0.50 protein_coding upstream_gene_variant MODIFIER -22A>T None
M0131985 MKMHILHB_00003 966 3 Oral 0.50 protein_coding upstream_gene_variant MODIFIER -21C>T None
M0131986 MKMHILHB_00003 967 3 Oral 0.50 protein_coding upstream_gene_variant MODIFIER -20C>A None
M0131987 MKMHILHB_00003 986 3 Oral 0.50 protein_coding upstream_gene_variant MODIFIER -1A>G None
M0131988 MKMHILHB_00004 3210 4 Oral 0.67 protein_coding missense_variant MODERATE 841G>A Val281Met
M0131989 MKMHILHB_00004 3239 3 Oral 0.50 protein_coding synonymous_variant LOW 870T>C Pro290Pro
M0131990 MKMHILHB_00004 3890 3 Oral 0.50 protein_coding synonymous_variant LOW 1521C>T Thr507Thr
M0131991 MKMHILHB_00004 3914 3 Oral 0.50 protein_coding synonymous_variant LOW 1545T>C Ala515Ala
M0131992 MKMHILHB_00004 7871 3 Oral 0.50 protein_coding downstream_gene_variant MODIFIER *3216C>T None






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term