Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C77
  Reference Plasmid   CP033372.1
  Reference Plasmid Size   759694
  Reference Plasmid GC Content   0.50
  Reference Plasmid Mobility Type   mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0143654 FEOLHBOC_00129 114493 4 Gut 0.10 protein_coding missense_variant MODERATE 7T>G Cys3Gly
M0143655 FEOLHBOC_00133 117424 4 Gut 0.10 protein_coding missense_variant MODERATE 506T>C Val169Ala
M0143656 FEOLHBOC_00135 119272 4 Gut 0.10 protein_coding synonymous_variant LOW 624G>T Gly208Gly
M0143657 FEOLHBOC_00137 122732 4 Gut 0.10 protein_coding missense_variant MODERATE 2437G>C Gly813Arg
M0143658 FEOLHBOC_00139 124314 4 Gut 0.10 protein_coding synonymous_variant LOW 508T>C Leu170Leu
M0143659 FEOLHBOC_00139 125097 4 Gut 0.10 protein_coding missense_variant MODERATE 1291A>G Thr431Ala
M0143660 FEOLHBOC_00140 128079 4 Gut 0.10 protein_coding missense_variant MODERATE 663A>C Lys221Asn
M0143661 FEOLHBOC_00143 132233 4 Gut 0.10 protein_coding missense_variant MODERATE 218C>A Pro73His
M0143662 FEOLHBOC_00147 136703 3 Gut 0.07 protein_coding synonymous_variant LOW 1375C>T Leu459Leu
M0143663 FEOLHBOC_00144 137453 3 Gut 0.07 protein_coding upstream_gene_variant MODIFIER -3410T>C None
M0143664 FEOLHBOC_00144 137505 3 Gut 0.07 protein_coding upstream_gene_variant MODIFIER -3462T>C None
M0143665 FEOLHBOC_00144 137519 3 Gut 0.07 protein_coding upstream_gene_variant MODIFIER -3476C>T None
M0143666 FEOLHBOC_00144 137777 3 Gut 0.07 protein_coding upstream_gene_variant MODIFIER -3734A>G None
M0143667 FEOLHBOC_00149 138180 3 Gut 0.07 protein_coding synonymous_variant LOW 21C>T Gly7Gly
M0143668 FEOLHBOC_00149 138189 3 Gut 0.07 protein_coding synonymous_variant LOW 30T>C Val10Val
M0143669 FEOLHBOC_00149 138534 4 Gut 0.10 protein_coding synonymous_variant LOW 375C>T His125His
M0143670 FEOLHBOC_00149 138540 4 Gut 0.10 protein_coding synonymous_variant LOW 381T>C Asp127Asp
M0143671 FEOLHBOC_00149 138543 4 Gut 0.10 protein_coding synonymous_variant LOW 384T>C Gly128Gly
M0143672 FEOLHBOC_00149 138681 3 Gut 0.07 protein_coding synonymous_variant LOW 522G>A Leu174Leu
M0143673 FEOLHBOC_00150 139350 3 Gut 0.07 protein_coding synonymous_variant LOW 510G>A Leu170Leu
M0143674 FEOLHBOC_00155 141647 3 Gut 0.07 protein_coding synonymous_variant LOW 321A>G Lys107Lys
M0143675 FEOLHBOC_00161 143576 3 Gut 0.07 protein_coding synonymous_variant LOW 54C>T Asn18Asn
M0143676 FEOLHBOC_00162 144215 3 Gut 0.07 protein_coding missense_variant MODERATE 502T>C Tyr168His
M0143677 FEOLHBOC_00164 144689 3 Gut 0.07 protein_coding missense_variant MODERATE 199A>G Ser67Gly
M0143678 FEOLHBOC_00165 144945 3 Gut 0.07 protein_coding missense_variant MODERATE 28A>G Asn10Asp
M0143679 FEOLHBOC_00166 145069 3 Gut 0.07 protein_coding synonymous_variant LOW 27A>G Lys9Lys
M0143680 FEOLHBOC_00168 146233 3 Gut 0.07 protein_coding synonymous_variant LOW 348C>T Asn116Asn
M0143681 FEOLHBOC_00170 146739 3 Gut 0.07 protein_coding synonymous_variant LOW 117A>G Lys39Lys
M0143682 FEOLHBOC_00177 151078 4 Gut 0.10 protein_coding synonymous_variant LOW 633C>T Ser211Ser
M0143683 FEOLHBOC_00178 151503 3 Gut 0.07 protein_coding missense_variant MODERATE 81G>T Lys27Asn
M0143684 FEOLHBOC_00181 154447 3 Gut 0.07 protein_coding missense_variant MODERATE 787A>G Thr263Ala
M0143685 FEOLHBOC_00182 154706 3 Gut 0.07 protein_coding missense_variant MODERATE 227T>C Leu76Pro
M0143686 FEOLHBOC_00182 155022 3 Gut 0.07 protein_coding synonymous_variant LOW 543C>T Asp181Asp
M0143687 FEOLHBOC_00182 155103 3 Gut 0.07 protein_coding synonymous_variant LOW 624G>A Leu208Leu
M0143688 FEOLHBOC_00106 97529 4 Gut 0.10 protein_coding synonymous_variant LOW 57G>A Leu19Leu
M0143689 FEOLHBOC_00106 98126 4 Gut 0.10 protein_coding synonymous_variant LOW 654C>T His218His
M0143690 FEOLHBOC_00093 83800 3 Gut 0.07 protein_coding synonymous_variant LOW 1983T>C Tyr661Tyr
M0143691 FEOLHBOC_00093 84229 3 Gut 0.07 protein_coding synonymous_variant LOW 1554T>C Phe518Phe
M0143692 FEOLHBOC_00093 84879 3 Gut 0.07 protein_coding missense_variant MODERATE 904C>G Leu302Val
M0143693 FEOLHBOC_00093 85054 3 Gut 0.07 protein_coding synonymous_variant LOW 729T>C Val243Val
M0143694 FEOLHBOC_00093 85168 3 Gut 0.07 protein_coding synonymous_variant LOW 615A>G Leu205Leu
M0143695 FEOLHBOC_00093 85863 3 Gut 0.07 protein_coding upstream_gene_variant MODIFIER -81T>C None
M0143696 FEOLHBOC_00096 87365 3 Gut 0.07 protein_coding synonymous_variant LOW 195A>C Ile65Ile
M0143697 FEOLHBOC_00096 88654 3 Gut 0.07 protein_coding missense_variant MODERATE 1484A>C Asn495Thr
M0143698 FEOLHBOC_00099 91261 3 Gut 0.07 protein_coding missense_variant MODERATE 362T>C Val121Ala
M0143699 FEOLHBOC_00099 92182 3 Gut 0.07 protein_coding missense_variant MODERATE 1283A>G Asp428Gly
M0143700 FEOLHBOC_00100 92355 3 Gut 0.07 protein_coding upstream_gene_variant MODIFIER -119C>T None
M0143701 FEOLHBOC_00100 93708 3 Gut 0.07 protein_coding missense_variant MODERATE 1235T>C Leu412Pro
M0143702 FEOLHBOC_00100 93757 3 Gut 0.07 protein_coding synonymous_variant LOW 1284G>A Pro428Pro
M0143703 FEOLHBOC_00102 95654 3 Gut 0.07 protein_coding missense_variant MODERATE 75A>C Leu25Phe
M0143704 FEOLHBOC_00106 97715 3 Gut 0.07 protein_coding synonymous_variant LOW 243C>T His81His
M0143705 FEOLHBOC_00106 98897 3 Gut 0.07 protein_coding synonymous_variant LOW 1425A>G Ala475Ala
M0143706 FEOLHBOC_00107 99669 3 Gut 0.07 protein_coding missense_variant MODERATE 194T>C Val65Ala
M0143707 FEOLHBOC_00108 100791 3 Gut 0.07 protein_coding missense_variant MODERATE 383T>C Leu128Pro
M0143708 FEOLHBOC_00111 103038 3 Gut 0.07 protein_coding missense_variant MODERATE 334G>A Gly112Arg
M0143709 FEOLHBOC_00111 103370 3 Gut 0.07 protein_coding synonymous_variant LOW 666T>C Thr222Thr
M0143710 FEOLHBOC_00112 104374 3 Gut 0.07 protein_coding synonymous_variant LOW 87A>G Gly29Gly
M0143711 FEOLHBOC_00113 105007 3 Gut 0.07 protein_coding synonymous_variant LOW 396A>C Ala132Ala
M0143712 FEOLHBOC_00114 105496 3 Gut 0.07 protein_coding synonymous_variant LOW 54T>C Ile18Ile
M0143713 FEOLHBOC_00116 107641 3 Gut 0.07 protein_coding missense_variant MODERATE 347T>C Val116Ala
M0143714 FEOLHBOC_00116 107666 3 Gut 0.07 protein_coding synonymous_variant LOW 372A>G Ala124Ala
M0143715 FEOLHBOC_00116 108006 3 Gut 0.07 protein_coding missense_variant MODERATE 712T>A Phe238Ile
M0143716 FEOLHBOC_00119 109943 3 Gut 0.07 protein_coding synonymous_variant LOW 351T>C Ala117Ala
M0143717 FEOLHBOC_00119 110109 3 Gut 0.07 protein_coding missense_variant MODERATE 185T>C Val62Ala
M0143718 FEOLHBOC_00120 110691 3 Gut 0.07 protein_coding synonymous_variant LOW 354T>G Val118Val






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition
FEOLHBOC_00492 VFG006826 LisR/LisK 71.9 1.2e-91 1 228 1.0 1.0088 Regulation two-component response regulator prediction







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group
FEOLHBOC_00889 Copper (Cu) 77.2 3.7e-62 1 149 0.9803 0.9803 prediction






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism
FEOLHBOC_00168 ARO:3007527 71.4 2.45e-79 7 167 0.9527 0.9699 aminoglycoside antibiotic spectinomycin resistant rpsE antibiotic target alteration






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant
FEOLHBOC_00114 PHI:7637 CspA 86.4 1.4e-27 1 66 1.0000 1.0000 bony fishes listeriosis cold shock protein reduced virulence
FEOLHBOC_00371 PHI:4127 bgsB 82.2 6.3e-16 1 45 0.5844 0.8428 rodents enterococcal endocarditis cell-wall glycolipids reduced virulence






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
FEOLHBOC_00027 AGL89660.1|GH73 100 5.58e-136 1 195 1 1
FEOLHBOC_00140 CCO12868.2|GH1 74.1 0 1 862 0.9588 0.7152
FEOLHBOC_00188 AKM51856.1|GH73 100 4.38e-142 1 204 1 1
FEOLHBOC_00313 AYP99083.1|GT111 100 1.93e-192 1 253 1 1
FEOLHBOC_00320 WGW21413.1|GH73 99.5 1.08e-163 2 370 0.9973 0.9584
FEOLHBOC_00341 UJP15443.1|GT2 100 1.14e-227 1 325 1 1
FEOLHBOC_00344 AOR73897.1|GT113 100 7.33e-273 1 350 1 1
FEOLHBOC_00345 QID93864.1|GT2 100 5.03e-224 1 302 1 1
FEOLHBOC_00372 UZM84849.1|GT4 97.8 6.01e-222 1 319 0.9785 0.7877
FEOLHBOC_00373 AKM51739.1|GT4 100 3.55e-255 1 341 1 1
FEOLHBOC_00436 QZY75998.1|GH73 100 3.41e-239 1 372 1 1
FEOLHBOC_00452 AUO28086.1|GT51 100 0 1 705 1 0.9986
FEOLHBOC_00620 AGL62902.2|GH36 97.4 9.31e-219 1 309 1 1
FEOLHBOC_00626 AGL62902.2|GH36 99.4 2.49e-223 1 309 1 1
FEOLHBOC_00711 QIX58845.1|GT2 100 5.65e-137 1 188 1 1
FEOLHBOC_00903 AYP99429.1|GT32 100 5.28e-174 1 239 1 1





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
FEOLHBOC_00234 2.A.7.5.3 95.3 7.2e-153 1 295 1.0000 1.0000 2 Electrochemical Potential-driven Transporters 2.A Porters (uniporters, symporters, antiporters) 2.A.7 The Drug/Metabolite Transporter (DMT) Superfamily
FEOLHBOC_00308 9.B.126.2.5 99.3 7.3e-161 1 298 1.0000 0.9967 9 Incompletely Characterized Transport Systems 9.B Putative transport proteins 9.B.126 The Putative Lipid IV Exporter (YhjD) Family