Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C153
  Reference Plasmid   CP088772.1
  Reference Plasmid Size   211329
  Reference Plasmid GC Content   0.47
  Reference Plasmid Mobility Type   conjugative





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0145488 KKOLJHFE_00055 52066 3 Gut 0.11 protein_coding upstream_gene_variant MODIFIER -4573T>C None
M0145489 KKOLJHFE_00055 52187 3 Gut 0.11 protein_coding upstream_gene_variant MODIFIER -4694A>C None
M0145490 KKOLJHFE_00061 52200 3 Gut 0.11 protein_coding missense_variant MODERATE 146C>G Ala49Gly
M0145491 KKOLJHFE_00057 52816 3 Gut 0.11 protein_coding upstream_gene_variant MODIFIER -4882A>G None
M0145492 KKOLJHFE_00057 52825 3 Gut 0.11 protein_coding upstream_gene_variant MODIFIER -4891G>T None
M0145493 KKOLJHFE_00052 44148 3 Gut 0.11 protein_coding missense_variant MODERATE 432G>A Met144Ile
M0145494 KKOLJHFE_00052 44285 3 Gut 0.11 protein_coding missense_variant MODERATE 295G>A Glu99Lys
M0145495 KKOLJHFE_00052 44297 3 Gut 0.11 protein_coding missense_variant MODERATE 283T>C Ser95Pro
M0145496 KKOLJHFE_00052 44315 3 Gut 0.11 protein_coding missense_variant MODERATE 265T>G Ser89Ala
M0145497 KKOLJHFE_00052 44323 3 Gut 0.11 protein_coding missense_variant MODERATE 257A>C Lys86Thr
M0145498 KKOLJHFE_00052 44324 3 Gut 0.11 protein_coding stop_gained HIGH 256A>T Lys86*
M0145499 KKOLJHFE_00052 44388 3 Gut 0.11 protein_coding synonymous_variant LOW 192C>T Cys64Cys
M0145500 KKOLJHFE_00052 44466 3 Gut 0.11 protein_coding synonymous_variant LOW 114T>C Ser38Ser
M0145501 KKOLJHFE_00052 44482 3 Gut 0.11 protein_coding missense_variant MODERATE 98T>G Ile33Ser
M0145502 KKOLJHFE_00052 44558 3 Gut 0.11 protein_coding missense_variant MODERATE 22T>C Tyr8His
M0145503 KKOLJHFE_00053 44914 3 Gut 0.11 protein_coding synonymous_variant LOW 123T>A Thr41Thr
M0145504 KKOLJHFE_00053 44954 3 Gut 0.11 protein_coding missense_variant MODERATE 83A>G Asn28Ser
M0145505 KKOLJHFE_00047 45334 3 Gut 0.11 protein_coding upstream_gene_variant MODIFIER -3765A>G None
M0145506 KKOLJHFE_00047 45399 3 Gut 0.11 protein_coding upstream_gene_variant MODIFIER -3830C>T None
M0145507 KKOLJHFE_00047 45410 3 Gut 0.11 protein_coding upstream_gene_variant MODIFIER -3841G>A None
M0145508 KKOLJHFE_00047 45412 3 Gut 0.11 protein_coding upstream_gene_variant MODIFIER -3843T>C None
M0145509 KKOLJHFE_00047 45442 3 Gut 0.11 protein_coding upstream_gene_variant MODIFIER -3873C>T None
M0145510 KKOLJHFE_00047 45448 3 Gut 0.11 protein_coding upstream_gene_variant MODIFIER -3879T>C None
M0145511 KKOLJHFE_00047 45480 3 Gut 0.11 protein_coding upstream_gene_variant MODIFIER -3911T>C None
M0145512 KKOLJHFE_00047 45539 3 Gut 0.11 protein_coding upstream_gene_variant MODIFIER -3970A>T None
M0145513 KKOLJHFE_00047 45560 3 Gut 0.11 protein_coding upstream_gene_variant MODIFIER -3991T>G None
M0145514 KKOLJHFE_00054 45694 3 Gut 0.11 protein_coding synonymous_variant LOW 60A>G Lys20Lys
M0145515 KKOLJHFE_00054 45833 3 Gut 0.11 protein_coding missense_variant MODERATE 199C>A Gln67Lys
M0145516 KKOLJHFE_00054 45859 3 Gut 0.11 protein_coding synonymous_variant LOW 225G>A Leu75Leu
M0145517 KKOLJHFE_00017 12845 4 Gut 0.14 protein_coding synonymous_variant LOW 573T>A Ala191Ala
M0145518 KKOLJHFE_00018 13579 3 Gut 0.11 protein_coding missense_variant MODERATE 449T>G Phe150Cys
M0145519 KKOLJHFE_00018 13802 3 Gut 0.11 protein_coding missense_variant MODERATE 226A>G Ile76Val
M0145520 KKOLJHFE_00011 14130 3 Gut 0.11 protein_coding upstream_gene_variant MODIFIER -4611G>C None
M0145521 KKOLJHFE_00019 14358 4 Gut 0.14 protein_coding synonymous_variant LOW 190T>C Leu64Leu
M0145522 KKOLJHFE_00020 15265 4 Gut 0.14 protein_coding synonymous_variant LOW 378T>G Pro126Pro
M0145523 KKOLJHFE_00020 15358 4 Gut 0.14 protein_coding synonymous_variant LOW 471T>C Tyr157Tyr
M0145524 KKOLJHFE_00020 16325 4 Gut 0.14 protein_coding missense_variant MODERATE 1438T>C Ser480Pro
M0145525 KKOLJHFE_00014 16508 3 Gut 0.11 protein_coding upstream_gene_variant MODIFIER -4602A>G None
M0145526 KKOLJHFE_00027 23888 4 Gut 0.14 protein_coding missense_variant MODERATE 243G>T Lys81Asn
M0145527 KKOLJHFE_00028 24357 3 Gut 0.11 protein_coding missense_variant MODERATE 383A>G Glu128Gly
M0145528 KKOLJHFE_00126 105193 3 Gut 0.11 protein_coding upstream_gene_variant MODIFIER -2670G>A None
M0145529 KKOLJHFE_00126 105211 3 Gut 0.11 protein_coding upstream_gene_variant MODIFIER -2688A>G None
M0145530 KKOLJHFE_00126 105266 6 Gut 0.21 protein_coding upstream_gene_variant MODIFIER -2743C>T None
M0145531 KKOLJHFE_00126 105420 3 Gut 0.11 protein_coding upstream_gene_variant MODIFIER -2897G>A None
M0145532 KKOLJHFE_00126 105507 3 Gut 0.11 protein_coding upstream_gene_variant MODIFIER -2984G>A None
M0145533 KKOLJHFE_00126 105508 3 Gut 0.11 protein_coding upstream_gene_variant MODIFIER -2985G>T None
M0145534 KKOLJHFE_00126 105531 3 Gut 0.11 protein_coding upstream_gene_variant MODIFIER -3008T>C None
M0145535 KKOLJHFE_00126 105539 3 Gut 0.11 protein_coding upstream_gene_variant MODIFIER -3016G>A None
M0145536 KKOLJHFE_00126 105603 3 Gut 0.11 protein_coding upstream_gene_variant MODIFIER -3080T>A None
M0145537 KKOLJHFE_00126 105837 5 Gut 0.18 protein_coding upstream_gene_variant MODIFIER -3314C>T None
M0145538 KKOLJHFE_00133 107097 5 Gut 0.18 protein_coding synonymous_variant LOW 246A>G Leu82Leu
M0145539 KKOLJHFE_00133 107281 5 Gut 0.18 protein_coding missense_variant MODERATE 62A>G Asp21Gly
M0145540 KKOLJHFE_00132 110896 4 Gut 0.14 protein_coding upstream_gene_variant MODIFIER -4107A>C None
M0145541 KKOLJHFE_00139 115753 5 Gut 0.18 protein_coding synonymous_variant LOW 1035T>C Arg345Arg
M0145542 KKOLJHFE_00139 120555 5 Gut 0.18 protein_coding upstream_gene_variant MODIFIER -3768A>G None
M0145543 KKOLJHFE_00143 120783 4 Gut 0.14 protein_coding missense_variant MODERATE 437T>C Met146Thr
M0145544 KKOLJHFE_00145 122643 5 Gut 0.18 protein_coding synonymous_variant LOW 345C>T Asn115Asn
M0145545 KKOLJHFE_00145 122769 3 Gut 0.11 protein_coding synonymous_variant LOW 219T>G Leu73Leu
M0145546 KKOLJHFE_00145 122778 3 Gut 0.11 protein_coding synonymous_variant LOW 210T>G Leu70Leu
M0145547 KKOLJHFE_00145 122801 3 Gut 0.11 protein_coding missense_variant MODERATE 187A>G Thr63Ala
M0145548 KKOLJHFE_00019 14696 3 Gut 0.11 protein_coding synonymous_variant LOW 528G>T Gly176Gly
M0145549 KKOLJHFE_00092 71786 3 Gut 0.11 protein_coding synonymous_variant LOW 375T>C Tyr125Tyr
M0145550 KKOLJHFE_00091 72167 3 Gut 0.11 protein_coding upstream_gene_variant MODIFIER -1435T>G None
M0145551 KKOLJHFE_00129 103647 3 Gut 0.11 protein_coding synonymous_variant LOW 762T>C Ala254Ala
M0145552 KKOLJHFE_00146 124375 3 Gut 0.11 protein_coding synonymous_variant LOW 3630C>T Gly1210Gly
M0145553 KKOLJHFE_00146 124377 3 Gut 0.11 protein_coding missense_variant MODERATE 3628G>A Gly1210Ser
M0145554 KKOLJHFE_00146 124462 3 Gut 0.11 protein_coding synonymous_variant LOW 3543A>G Gly1181Gly
M0145555 KKOLJHFE_00146 124687 3 Gut 0.11 protein_coding missense_variant MODERATE 3318C>A Asn1106Lys
M0145556 KKOLJHFE_00146 124691 3 Gut 0.11 protein_coding missense_variant MODERATE 3314A>T Gln1105Leu
M0145557 KKOLJHFE_00146 124816 3 Gut 0.11 protein_coding synonymous_variant LOW 3189A>G Gly1063Gly
M0145558 KKOLJHFE_00146 124897 3 Gut 0.11 protein_coding synonymous_variant LOW 3108A>G Lys1036Lys
M0145559 KKOLJHFE_00146 125005 3 Gut 0.11 protein_coding synonymous_variant LOW 3000C>T Gly1000Gly
M0145560 KKOLJHFE_00146 125014 3 Gut 0.11 protein_coding synonymous_variant LOW 2991A>G Ala997Ala
M0145561 KKOLJHFE_00146 125221 3 Gut 0.11 protein_coding synonymous_variant LOW 2784A>T Arg928Arg
M0145562 KKOLJHFE_00146 125237 3 Gut 0.11 protein_coding missense_variant MODERATE 2768C>A Ala923Glu
M0145563 KKOLJHFE_00146 125365 3 Gut 0.11 protein_coding synonymous_variant LOW 2640G>A Ser880Ser
M0145564 KKOLJHFE_00146 125368 3 Gut 0.11 protein_coding synonymous_variant LOW 2637G>A Thr879Thr
M0145565 KKOLJHFE_00146 125375 3 Gut 0.11 protein_coding missense_variant MODERATE 2630C>A Thr877Lys
M0145566 KKOLJHFE_00146 125392 3 Gut 0.11 protein_coding synonymous_variant LOW 2613C>T Pro871Pro
M0145567 KKOLJHFE_00146 125452 3 Gut 0.11 protein_coding synonymous_variant LOW 2553T>C Ser851Ser
M0145568 KKOLJHFE_00146 125455 3 Gut 0.11 protein_coding synonymous_variant LOW 2550A>G Lys850Lys
M0145569 KKOLJHFE_00146 125599 4 Gut 0.14 protein_coding synonymous_variant LOW 2406T>G Ser802Ser
M0145570 KKOLJHFE_00146 126159 3 Gut 0.11 protein_coding missense_variant MODERATE 1846T>C Ser616Pro
M0145571 KKOLJHFE_00146 126526 3 Gut 0.11 protein_coding synonymous_variant LOW 1479T>C Ala493Ala
M0145572 KKOLJHFE_00146 126631 3 Gut 0.11 protein_coding synonymous_variant LOW 1374T>C Ala458Ala
M0145573 KKOLJHFE_00148 128879 3 Gut 0.11 protein_coding synonymous_variant LOW 1677T>C Ser559Ser
M0145574 KKOLJHFE_00148 129443 3 Gut 0.11 protein_coding synonymous_variant LOW 1113T>G Arg371Arg
M0145575 KKOLJHFE_00148 129743 3 Gut 0.11 protein_coding synonymous_variant LOW 813C>T Gly271Gly
M0145576 KKOLJHFE_00148 129749 3 Gut 0.11 protein_coding synonymous_variant LOW 807G>T Pro269Pro
M0145577 KKOLJHFE_00148 129956 3 Gut 0.11 protein_coding synonymous_variant LOW 600A>G Glu200Glu
M0145578 KKOLJHFE_00126 105706 3 Gut 0.11 protein_coding upstream_gene_variant MODIFIER -3183C>T None
M0145579 KKOLJHFE_00126 105760 3 Gut 0.11 protein_coding upstream_gene_variant MODIFIER -3237T>G None
M0145580 KKOLJHFE_00140 116886 3 Gut 0.11 protein_coding missense_variant MODERATE 1204A>G Ser402Gly
M0145581 KKOLJHFE_00139 120577 3 Gut 0.11 protein_coding upstream_gene_variant MODIFIER -3790T>C None
M0145582 KKOLJHFE_00146 127180 3 Gut 0.11 protein_coding synonymous_variant LOW 825T>C Ser275Ser
M0145583 KKOLJHFE_00146 127309 3 Gut 0.11 protein_coding synonymous_variant LOW 696C>G Ala232Ala
M0145584 KKOLJHFE_00146 127354 3 Gut 0.11 protein_coding synonymous_variant LOW 651A>T Pro217Pro
M0145585 KKOLJHFE_00146 127364 3 Gut 0.11 protein_coding missense_variant MODERATE 641T>C Val214Ala
M0145586 KKOLJHFE_00146 127540 3 Gut 0.11 protein_coding synonymous_variant LOW 465T>G Gly155Gly
M0145587 KKOLJHFE_00148 128434 3 Gut 0.11 protein_coding missense_variant MODERATE 2122C>A Leu708Ile






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition
KKOLJHFE_00125 VFG034574 Dispersin 95.7 1.1e-60 1 116 1.0 1 Others dispersin experiment
KKOLJHFE_00002 VFG035904 Dispersin 83.2 2.8e-162 6 377 0.9867 0.9841 Others permease AatP prediction
KKOLJHFE_00003 VFG035911 Dispersin 80.2 1.5e-177 1 400 1.0 1 Others outer membrane protein AatA prediction
KKOLJHFE_00004 VFG035918 Dispersin 82.4 1.7e-118 3 257 0.9922 1 Others AatB prediction
KKOLJHFE_00005 VFG035924 Dispersin 82.2 1.5e-93 1 208 1.0 1 Others ATP-binding protein AatC prediction
KKOLJHFE_00023 VFG033834 Pic 98.8 0 1 1364 1.0 0.9985 Effector delivery system Pic serine protease precursor, autotransporter prediction
KKOLJHFE_00029 VFG041000 AAI/SCI-II T6SS 92.6 3.7e-99 1 188 1.0 1 Effector delivery system hypothetical protein prediction
KKOLJHFE_00109 VFG035394 AAI/SCI-II T6SS 72.7 1.7e-39 1 99 1.0 1 Effector delivery system PAAR domain-containing protein prediction
KKOLJHFE_00116 VFG035359 AAI/SCI-II T6SS 80.1 7.6e-237 1 492 1.0 1 Effector delivery system type VI secretion system contractile sheath large subunit prediction
KKOLJHFE_00125 VFG034574 Dispersin 95.7 8e-60 1 116 1.0 1 Others dispersin prediction
KKOLJHFE_00202 VFG042364 Hbp (hemoglobin-binding protease) 70.2 0 1 1149 0.8866 0.8344 Effector delivery system haemoglobin protease prediction
KKOLJHFE_00206 VFG034602 Adhesive fimbriae 93 1.7e-139 1 257 1.0 1 Adherence fimbrial protein FaeJ prediction
KKOLJHFE_00207 VFG043644 CS31A capsule-like antigen 95.3 6.5e-139 1 254 1.0 1 Adherence CS31A minor subunit prediction
KKOLJHFE_00208 VFG043647 CS31A capsule-like antigen 91.7 1.4e-136 1 265 1.0 0.9962 Adherence fimbrial protein prediction
KKOLJHFE_00210 VFG034598 Adhesive fimbriae 92.6 3.8e-76 1 163 1.0 1 Adherence fimbrial protein FaeF prediction
KKOLJHFE_00211 VFG043643 CS31A capsule-like antigen 97 9e-144 1 263 1.0 1 Adherence unknown protein prediction
KKOLJHFE_00212 VFG034596 Adhesive fimbriae 93.6 0 5 814 0.9951 0.9988 Adherence fimbrial usher protein FaeD prediction
KKOLJHFE_00213 VFG042533 CS31A capsule-like antigen 88.9 2.2e-85 1 178 1.0 0.9889 Adherence ClpC prediction







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
KKOLJHFE_00169 QJS03103.1|GH23 100 1e-123 1 169 1 1





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
KKOLJHFE_00136 1.E.53.1.11 100 1.1e-21 1 49 1.0000 0.7206 1 Channels/Pores 1.E Holins 1.E.53 The Toxic Hok/Gef Protein (Hok/Gef) Family
KKOLJHFE_00202 1.B.12.4.2 70.2 0 1 1149 0.8866 0.8562 1 Channels/Pores 1.B β-Barrel Porins 1.B.12 The Autotransporter-1 (AT-1) Family
KKOLJHFE_00212 1.B.11.1.1 93.3 0 5 814 0.9951 0.9975 1 Channels/Pores 1.B β-Barrel Porins 1.B.11 The Outer Membrane Fimbrial Usher Porin (FUP) Family