Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C175
  Reference Plasmid   CP103105.1
  Reference Plasmid Size   35561
  Reference Plasmid GC Content   0.32
  Reference Plasmid Mobility Type   conjugative





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0146359 ECHBKFNO_00002 518 9 Gut 0.64 protein_coding synonymous_variant LOW 180G>A Gly60Gly
M0146360 ECHBKFNO_00003 570 4 Gut 0.29 protein_coding upstream_gene_variant MODIFIER -6A>C None
M0146361 ECHBKFNO_00003 751 8 Gut 0.57 protein_coding missense_variant MODERATE 176A>G Asp59Gly
M0146362 ECHBKFNO_00004 1015 8 Gut 0.57 protein_coding missense_variant MODERATE 89T>A Val30Asp
M0146363 ECHBKFNO_00004 1043 6 Gut 0.43 protein_coding synonymous_variant LOW 117G>A Gly39Gly
M0146364 ECHBKFNO_00004 1144 8 Gut 0.57 protein_coding missense_variant MODERATE 218T>A Phe73Tyr
M0146365 ECHBKFNO_00004 1157 8 Gut 0.57 protein_coding synonymous_variant LOW 231A>G Glu77Glu
M0146366 ECHBKFNO_00006 1771 8 Gut 0.57 protein_coding upstream_gene_variant MODIFIER -3A>G None
M0146367 ECHBKFNO_00006 2002 8 Gut 0.57 protein_coding missense_variant MODERATE 229G>A Asp77Asn
M0146368 ECHBKFNO_00006 2070 8 Gut 0.57 protein_coding synonymous_variant LOW 297T>C Ser99Ser
M0146369 ECHBKFNO_00006 2187 4 Gut 0.29 protein_coding synonymous_variant LOW 414T>G Pro138Pro
M0146370 ECHBKFNO_00006 2194 8 Gut 0.57 protein_coding missense_variant MODERATE 421C>T Leu141Phe
M0146371 ECHBKFNO_00006 2226 8 Gut 0.57 protein_coding missense_variant MODERATE 453T>A Asp151Glu
M0146372 ECHBKFNO_00006 2883 8 Gut 0.57 protein_coding synonymous_variant LOW 1110A>G Glu370Glu
M0146373 ECHBKFNO_00007 2952 8 Gut 0.57 protein_coding upstream_gene_variant MODIFIER -1111G>A None
M0146374 ECHBKFNO_00007 2954 6 Gut 0.43 protein_coding upstream_gene_variant MODIFIER -1109A>C None
M0146375 ECHBKFNO_00007 2959 4 Gut 0.29 protein_coding upstream_gene_variant MODIFIER -1104T>G None
M0146376 ECHBKFNO_00007 3059 8 Gut 0.57 protein_coding upstream_gene_variant MODIFIER -1004C>T None
M0146377 ECHBKFNO_00007 3060 8 Gut 0.57 protein_coding upstream_gene_variant MODIFIER -1003A>G None
M0146378 ECHBKFNO_00007 3150 8 Gut 0.57 protein_coding upstream_gene_variant MODIFIER -913T>C None
M0146379 ECHBKFNO_00007 3217 6 Gut 0.43 protein_coding upstream_gene_variant MODIFIER -846G>A None
M0146380 ECHBKFNO_00007 3313 8 Gut 0.57 protein_coding upstream_gene_variant MODIFIER -750A>G None
M0146381 ECHBKFNO_00007 3332 4 Gut 0.29 protein_coding upstream_gene_variant MODIFIER -731G>T None
M0146382 ECHBKFNO_00007 3365 4 Gut 0.29 protein_coding upstream_gene_variant MODIFIER -698G>A None
M0146383 ECHBKFNO_00043 35329 4 Gut 0.29 protein_coding upstream_gene_variant MODIFIER -4020G>C None
M0146384 ECHBKFNO_00043 35337 4 Gut 0.29 protein_coding upstream_gene_variant MODIFIER -4028C>T None
M0146385 ECHBKFNO_00034 23328 3 Gut 0.21 protein_coding synonymous_variant LOW 591C>T Phe197Phe
M0146386 ECHBKFNO_00034 23679 3 Gut 0.21 protein_coding synonymous_variant LOW 240G>A Lys80Lys
M0146387 ECHBKFNO_00035 23962 3 Gut 0.21 protein_coding synonymous_variant LOW 1068C>T Asn356Asn
M0146388 ECHBKFNO_00035 24028 3 Gut 0.21 protein_coding synonymous_variant LOW 1002C>T Ala334Ala
M0146389 ECHBKFNO_00035 24187 3 Gut 0.21 protein_coding synonymous_variant LOW 843T>C Ser281Ser
M0146390 ECHBKFNO_00035 24209 3 Gut 0.21 protein_coding missense_variant MODERATE 821G>A Arg274Lys
M0146391 ECHBKFNO_00035 24253 3 Gut 0.21 protein_coding synonymous_variant LOW 777T>C Ala259Ala
M0146392 ECHBKFNO_00035 24369 3 Gut 0.21 protein_coding synonymous_variant LOW 661T>C Leu221Leu
M0146393 ECHBKFNO_00035 24493 3 Gut 0.21 protein_coding synonymous_variant LOW 537A>G Gln179Gln
M0146394 ECHBKFNO_00035 24529 3 Gut 0.21 protein_coding synonymous_variant LOW 501A>G Leu167Leu
M0146395 ECHBKFNO_00035 24604 3 Gut 0.21 protein_coding synonymous_variant LOW 426G>A Arg142Arg
M0146396 ECHBKFNO_00036 25321 3 Gut 0.21 protein_coding synonymous_variant LOW 144T>C Asp48Asp
M0146397 ECHBKFNO_00036 25333 3 Gut 0.21 protein_coding synonymous_variant LOW 132G>A Leu44Leu
M0146398 ECHBKFNO_00036 25394 3 Gut 0.21 protein_coding missense_variant MODERATE 71C>T Ala24Val
M0146399 ECHBKFNO_00037 25503 3 Gut 0.21 protein_coding synonymous_variant LOW 576A>C Leu192Leu
M0146400 ECHBKFNO_00037 25608 3 Gut 0.21 protein_coding synonymous_variant LOW 471C>T Thr157Thr
M0146401 ECHBKFNO_00037 25617 3 Gut 0.21 protein_coding synonymous_variant LOW 462G>A Glu154Glu
M0146402 ECHBKFNO_00037 25665 3 Gut 0.21 protein_coding synonymous_variant LOW 414G>A Val138Val
M0146403 ECHBKFNO_00037 25667 3 Gut 0.21 protein_coding missense_variant MODERATE 412G>A Val138Met
M0146404 ECHBKFNO_00037 25716 3 Gut 0.21 protein_coding synonymous_variant LOW 363C>T Thr121Thr
M0146405 ECHBKFNO_00037 25866 3 Gut 0.21 protein_coding synonymous_variant LOW 213G>A Lys71Lys
M0146406 ECHBKFNO_00037 25908 3 Gut 0.21 protein_coding synonymous_variant LOW 171A>G Val57Val
M0146407 ECHBKFNO_00040 27862 3 Gut 0.21 protein_coding synonymous_variant LOW 414G>A Gly138Gly
M0146408 ECHBKFNO_00040 28066 3 Gut 0.21 protein_coding synonymous_variant LOW 210A>T Val70Val
M0146409 ECHBKFNO_00041 28283 3 Gut 0.21 protein_coding synonymous_variant LOW 177A>G Lys59Lys
M0146410 ECHBKFNO_00042 28560 3 Gut 0.21 protein_coding missense_variant MODERATE 603T>A Asp201Glu
M0146411 ECHBKFNO_00043 30311 3 Gut 0.21 protein_coding synonymous_variant LOW 999T>C Cys333Cys
M0146412 ECHBKFNO_00043 30401 3 Gut 0.21 protein_coding synonymous_variant LOW 909A>C Ile303Ile
M0146413 ECHBKFNO_00043 30598 3 Gut 0.21 protein_coding missense_variant MODERATE 712G>A Gly238Ser
M0146414 ECHBKFNO_00043 31241 3 Gut 0.21 protein_coding synonymous_variant LOW 69C>T Phe23Phe
M0146415 ECHBKFNO_00007 3088 4 Gut 0.29 protein_coding upstream_gene_variant MODIFIER -975C>T None
M0146416 ECHBKFNO_00007 3399 4 Gut 0.29 protein_coding upstream_gene_variant MODIFIER -664T>C None
M0146417 ECHBKFNO_00007 3415 4 Gut 0.29 protein_coding upstream_gene_variant MODIFIER -648C>A None
M0146418 ECHBKFNO_00007 3417 4 Gut 0.29 protein_coding upstream_gene_variant MODIFIER -646T>C None






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
ECHBKFNO_00021 9.B.154.1.1 95.2 3.1e-84 1 167 1.0000 1.0000 9 Incompletely Characterized Transport Systems 9.B Putative transport proteins 9.B.154 The Putative Holin-2 (PH-2) Family