Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C2994
  Reference Plasmid   DSKIN0031-1_bin.8__k141_7538
  Reference Plasmid Size   24533
  Reference Plasmid GC Content   0.70
  Reference Plasmid Mobility Type   non-mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0069390 DIIAMFKA_00017 17334 3 Skin 0.25 protein_coding missense_variant MODERATE 76G>A Ala26Thr
M0069391 DIIAMFKA_00017 17336 3 Skin 0.25 protein_coding synonymous_variant LOW 78A>C Ala26Ala
M0069392 DIIAMFKA_00017 17351 3 Skin 0.25 protein_coding synonymous_variant LOW 93G>A Ala31Ala
M0069393 DIIAMFKA_00017 17352 3 Skin 0.25 protein_coding missense_variant MODERATE 94G>C Ala32Pro
M0069394 DIIAMFKA_00017 17354 4 Skin 0.33 protein_coding synonymous_variant LOW 96A>G Ala32Ala
M0069395 DIIAMFKA_00017 17360 3 Skin 0.25 protein_coding synonymous_variant LOW 102A>C Ala34Ala
M0069396 DIIAMFKA_00017 17450 4 Skin 0.33 protein_coding synonymous_variant LOW 192T>C Tyr64Tyr
M0069397 DIIAMFKA_00017 17491 6 Skin 0.50 protein_coding missense_variant MODERATE 233T>G Leu78Arg
M0069398 DIIAMFKA_00017 17495 4 Skin 0.33 protein_coding synonymous_variant LOW 237T>C Pro79Pro
M0069399 DIIAMFKA_00017 17516 4 Skin 0.33 protein_coding synonymous_variant LOW 258T>C Gly86Gly
M0069400 DIIAMFKA_00017 17846 3 Skin 0.25 protein_coding synonymous_variant LOW 588G>A Lys196Lys
M0069401 DIIAMFKA_00019 19885 4 Skin 0.33 protein_coding synonymous_variant LOW 111G>T Arg37Arg
M0069402 DIIAMFKA_00019 20017 3 Skin 0.25 protein_coding upstream_gene_variant MODIFIER -22T>C None
M0069403 DIIAMFKA_00020 20588 4 Skin 0.33 protein_coding missense_variant MODERATE 491G>A Arg164Lys
M0069404 DIIAMFKA_00020 20592 4 Skin 0.33 protein_coding synonymous_variant LOW 495G>C Ser165Ser
M0069405 DIIAMFKA_00010 9410 4 Skin 0.33 protein_coding synonymous_variant LOW 1113C>G Ala371Ala
M0069406 DIIAMFKA_00010 9425 4 Skin 0.33 protein_coding synonymous_variant LOW 1098T>C His366His
M0069407 DIIAMFKA_00011 10853 3 Skin 0.25 protein_coding synonymous_variant LOW 720A>G Ala240Ala
M0069408 DIIAMFKA_00011 11066 3 Skin 0.25 protein_coding synonymous_variant LOW 507A>G Glu169Glu
M0069409 DIIAMFKA_00011 11081 3 Skin 0.25 protein_coding synonymous_variant LOW 492T>C His164His
M0069410 DIIAMFKA_00011 11192 5 Skin 0.42 protein_coding synonymous_variant LOW 381A>C Ser127Ser
M0069411 DIIAMFKA_00012 11757 7 Skin 0.58 protein_coding missense_variant MODERATE 697C>G Pro233Ala
M0069412 DIIAMFKA_00012 12249 4 Skin 0.33 protein_coding missense_variant MODERATE 205T>G Leu69Val
M0069413 DIIAMFKA_00012 12376 5 Skin 0.42 protein_coding synonymous_variant LOW 78G>C Thr26Thr
M0069414 DIIAMFKA_00007 12552 4 Skin 0.33 protein_coding upstream_gene_variant MODIFIER -4835T>C None
M0069415 DIIAMFKA_00013 12776 4 Skin 0.33 protein_coding synonymous_variant LOW 180T>C Tyr60Tyr
M0069416 DIIAMFKA_00013 12785 4 Skin 0.33 protein_coding synonymous_variant LOW 189T>C Gly63Gly
M0069417 DIIAMFKA_00013 12797 4 Skin 0.33 protein_coding synonymous_variant LOW 201C>T Asp67Asp
M0069418 DIIAMFKA_00013 12818 4 Skin 0.33 protein_coding synonymous_variant LOW 222C>G Ala74Ala
M0069419 DIIAMFKA_00013 13095 5 Skin 0.42 protein_coding missense_variant MODERATE 499C>T His167Tyr
M0069420 DIIAMFKA_00013 13097 5 Skin 0.42 protein_coding synonymous_variant LOW 501T>C His167His
M0069421 DIIAMFKA_00013 13397 4 Skin 0.33 protein_coding synonymous_variant LOW 801A>C Gly267Gly
M0069422 DIIAMFKA_00013 13589 3 Skin 0.25 protein_coding synonymous_variant LOW 993G>A Lys331Lys
M0069423 DIIAMFKA_00014 13834 6 Skin 0.50 protein_coding synonymous_variant LOW 144T>C Pro48Pro
M0069424 DIIAMFKA_00014 13900 6 Skin 0.50 protein_coding synonymous_variant LOW 210A>G Ala70Ala
M0069425 DIIAMFKA_00014 13960 6 Skin 0.50 protein_coding synonymous_variant LOW 270A>G Val90Val
M0069426 DIIAMFKA_00014 13987 4 Skin 0.33 protein_coding synonymous_variant LOW 297C>T Pro99Pro
M0069427 DIIAMFKA_00014 14008 4 Skin 0.33 protein_coding synonymous_variant LOW 318C>G Ala106Ala
M0069428 DIIAMFKA_00014 14011 4 Skin 0.33 protein_coding synonymous_variant LOW 321C>T Gly107Gly
M0069429 DIIAMFKA_00014 14020 4 Skin 0.33 protein_coding synonymous_variant LOW 330A>G Ala110Ala
M0069430 DIIAMFKA_00014 14074 4 Skin 0.33 protein_coding synonymous_variant LOW 384G>C Ala128Ala
M0069431 DIIAMFKA_00014 14932 3 Skin 0.25 protein_coding synonymous_variant LOW 1242T>C His414His
M0069432 DIIAMFKA_00014 14944 3 Skin 0.25 protein_coding synonymous_variant LOW 1254C>T His418His
M0069433 DIIAMFKA_00014 14977 3 Skin 0.25 protein_coding synonymous_variant LOW 1287C>T His429His
M0069434 DIIAMFKA_00014 15042 5 Skin 0.42 protein_coding missense_variant MODERATE 1352A>G Asp451Gly
M0069435 DIIAMFKA_00010 15196 3 Skin 0.25 protein_coding upstream_gene_variant MODIFIER -4674G>T None
M0069436 DIIAMFKA_00010 15212 5 Skin 0.42 protein_coding upstream_gene_variant MODIFIER -4690T>C None
M0069437 DIIAMFKA_00015 15227 5 Skin 0.42 protein_coding synonymous_variant LOW 6A>C Ala2Ala
M0069438 DIIAMFKA_00015 15230 5 Skin 0.42 protein_coding synonymous_variant LOW 9C>G Thr3Thr
M0069439 DIIAMFKA_00015 15232 5 Skin 0.42 protein_coding missense_variant MODERATE 11C>A Pro4His
M0069440 DIIAMFKA_00015 15587 4 Skin 0.33 protein_coding synonymous_variant LOW 366A>C Pro122Pro
M0069441 DIIAMFKA_00015 15824 4 Skin 0.33 protein_coding synonymous_variant LOW 603C>G Ala201Ala
M0069442 DIIAMFKA_00015 15875 4 Skin 0.33 protein_coding synonymous_variant LOW 654G>C Val218Val
M0069443 DIIAMFKA_00016 16172 5 Skin 0.42 protein_coding synonymous_variant LOW 117G>A Val39Val
M0069444 DIIAMFKA_00016 16430 4 Skin 0.33 protein_coding synonymous_variant LOW 375G>C Ala125Ala
M0069445 DIIAMFKA_00016 16748 4 Skin 0.33 protein_coding synonymous_variant LOW 693C>G Leu231Leu
M0069446 DIIAMFKA_00016 16822 3 Skin 0.25 protein_coding missense_variant MODERATE 767A>C Glu256Ala
M0069447 DIIAMFKA_00016 16829 4 Skin 0.33 protein_coding synonymous_variant LOW 774C>G Gly258Gly
M0069448 DIIAMFKA_00016 16947 3 Skin 0.25 protein_coding missense_variant MODERATE 892C>G Pro298Ala
M0069449 DIIAMFKA_00016 16964 3 Skin 0.25 protein_coding synonymous_variant LOW 909G>T Leu303Leu
M0069450 DIIAMFKA_00016 17134 4 Skin 0.33 protein_coding missense_variant MODERATE 1079G>A Arg360Lys
M0069451 DIIAMFKA_00012 12405 5 Skin 0.42 protein_coding missense_variant MODERATE 49C>G Leu17Val
M0069452 DIIAMFKA_00014 13883 4 Skin 0.33 protein_coding missense_variant MODERATE 193A>G Ile65Val
M0069453 DIIAMFKA_00015 15569 3 Skin 0.25 protein_coding synonymous_variant LOW 348C>T Ile116Ile
M0069454 DIIAMFKA_00015 15613 3 Skin 0.25 protein_coding missense_variant MODERATE 392G>A Arg131Gln
M0069455 DIIAMFKA_00016 16637 3 Skin 0.25 protein_coding synonymous_variant LOW 582C>T Ala194Ala
M0069456 DIIAMFKA_00017 17585 3 Skin 0.25 protein_coding synonymous_variant LOW 327G>C Ala109Ala
M0069457 DIIAMFKA_00017 17747 5 Skin 0.42 protein_coding synonymous_variant LOW 489G>A Lys163Lys
M0069458 DIIAMFKA_00017 17975 3 Skin 0.25 protein_coding synonymous_variant LOW 717C>G Arg239Arg
M0069459 DIIAMFKA_00017 17990 3 Skin 0.25 protein_coding synonymous_variant LOW 732C>G Pro244Pro
M0069460 DIIAMFKA_00019 19523 3 Skin 0.25 protein_coding missense_variant MODERATE 473A>C Asp158Ala
M0069461 DIIAMFKA_00021 21565 3 Skin 0.25 protein_coding synonymous_variant LOW 207G>C Val69Val
M0069462 DIIAMFKA_00021 21574 3 Skin 0.25 protein_coding synonymous_variant LOW 216G>A Glu72Glu
M0069463 DIIAMFKA_00022 23137 3 Skin 0.25 protein_coding missense_variant MODERATE 1385A>C Gln462Pro
M0069464 DIIAMFKA_00022 23463 3 Skin 0.25 protein_coding synonymous_variant LOW 1059G>C Ala353Ala
M0069465 DIIAMFKA_00007 10575 5 Skin 0.42 protein_coding upstream_gene_variant MODIFIER -2858G>T None
M0069466 DIIAMFKA_00007 10586 5 Skin 0.42 protein_coding upstream_gene_variant MODIFIER -2869A>G None
M0069467 DIIAMFKA_00011 10640 3 Skin 0.25 protein_coding synonymous_variant LOW 933T>C Ala311Ala
M0069468 DIIAMFKA_00008 13660 4 Skin 0.33 protein_coding upstream_gene_variant MODIFIER -4695C>T None
M0069469 DIIAMFKA_00008 13669 5 Skin 0.42 protein_coding upstream_gene_variant MODIFIER -4704T>C None
M0069470 DIIAMFKA_00016 17168 3 Skin 0.25 protein_coding synonymous_variant LOW 1113T>C Ser371Ser
M0069471 DIIAMFKA_00005 5539 4 Skin 0.33 protein_coding missense_variant MODERATE 447C>G Asp149Glu
M0069472 DIIAMFKA_00001 6125 3 Skin 0.25 protein_coding upstream_gene_variant MODIFIER -4786C>T None
M0069473 DIIAMFKA_00001 6128 3 Skin 0.25 protein_coding upstream_gene_variant MODIFIER -4789T>G None
M0069474 DIIAMFKA_00007 7031 3 Skin 0.25 protein_coding synonymous_variant LOW 687A>G Leu229Leu
M0069475 DIIAMFKA_00012 11971 3 Skin 0.25 protein_coding synonymous_variant LOW 483G>A Leu161Leu
M0069476 DIIAMFKA_00012 11980 3 Skin 0.25 protein_coding synonymous_variant LOW 474G>T Ala158Ala
M0069477 DIIAMFKA_00014 14506 3 Skin 0.25 protein_coding synonymous_variant LOW 816G>A Pro272Pro
M0069478 DIIAMFKA_00003 3425 3 Skin 0.25 protein_coding synonymous_variant LOW 324G>A Ala108Ala
M0069479 DIIAMFKA_00003 3565 3 Skin 0.25 protein_coding missense_variant MODERATE 184A>T Thr62Ser
M0069480 DIIAMFKA_00004 4801 3 Skin 0.25 protein_coding synonymous_variant LOW 996C>G Pro332Pro
M0069481 DIIAMFKA_00004 4831 3 Skin 0.25 protein_coding synonymous_variant LOW 1026A>C Gly342Gly
M0069482 DIIAMFKA_00004 4852 3 Skin 0.25 protein_coding synonymous_variant LOW 1047G>T Val349Val
M0069483 DIIAMFKA_00004 4957 4 Skin 0.33 protein_coding synonymous_variant LOW 1152A>G Glu384Glu
M0069484 DIIAMFKA_00005 5255 4 Skin 0.33 protein_coding missense_variant MODERATE 163C>A Leu55Ile
M0069485 DIIAMFKA_00005 5268 4 Skin 0.33 protein_coding missense_variant MODERATE 176G>T Arg59Met
M0069486 DIIAMFKA_00006 6699 3 Skin 0.25 protein_coding missense_variant MODERATE 482T>C Val161Ala
M0069487 DIIAMFKA_00003 2912 3 Skin 0.25 protein_coding synonymous_variant LOW 837T>C Ala279Ala
M0069488 DIIAMFKA_00003 2922 3 Skin 0.25 protein_coding missense_variant MODERATE 827G>A Arg276His






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term