Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C3030
  Reference Plasmid   DSKIN0049-1_bin.24__k141_100317
  Reference Plasmid Size   21923
  Reference Plasmid GC Content   0.67
  Reference Plasmid Mobility Type   non-mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0069948 MIKOKNHI_00019 20378 4 Skin 0.29 protein_coding synonymous_variant LOW 225T>C Val75Val
M0069949 MIKOKNHI_00015 20983 3 Skin 0.21 protein_coding upstream_gene_variant MODIFIER -3264G>T None
M0069950 MIKOKNHI_00015 20987 3 Skin 0.21 protein_coding upstream_gene_variant MODIFIER -3268T>C None
M0069951 MIKOKNHI_00010 11393 4 Skin 0.29 protein_coding synonymous_variant LOW 390G>A Glu130Glu
M0069952 MIKOKNHI_00010 11402 4 Skin 0.29 protein_coding synonymous_variant LOW 399C>G Ala133Ala
M0069953 MIKOKNHI_00010 11405 4 Skin 0.29 protein_coding synonymous_variant LOW 402G>T Gly134Gly
M0069954 MIKOKNHI_00010 11411 4 Skin 0.29 protein_coding synonymous_variant LOW 408T>C Gly136Gly
M0069955 MIKOKNHI_00010 11414 4 Skin 0.29 protein_coding synonymous_variant LOW 411A>G Ala137Ala
M0069956 MIKOKNHI_00010 11429 4 Skin 0.29 protein_coding synonymous_variant LOW 426T>C Thr142Thr
M0069957 MIKOKNHI_00010 11453 4 Skin 0.29 protein_coding synonymous_variant LOW 450T>C Gly150Gly
M0069958 MIKOKNHI_00010 11471 4 Skin 0.29 protein_coding synonymous_variant LOW 468C>G Ala156Ala
M0069959 MIKOKNHI_00012 13883 3 Skin 0.21 protein_coding synonymous_variant LOW 399C>T Asp133Asp
M0069960 MIKOKNHI_00012 13901 3 Skin 0.21 protein_coding synonymous_variant LOW 417C>T Asn139Asn
M0069961 MIKOKNHI_00012 13913 3 Skin 0.21 protein_coding synonymous_variant LOW 429G>C Arg143Arg
M0069962 MIKOKNHI_00012 13922 3 Skin 0.21 protein_coding synonymous_variant LOW 438C>T Ala146Ala
M0069963 MIKOKNHI_00012 13943 3 Skin 0.21 protein_coding synonymous_variant LOW 459G>A Val153Val
M0069964 MIKOKNHI_00012 13970 3 Skin 0.21 protein_coding synonymous_variant LOW 486C>T Gly162Gly
M0069965 MIKOKNHI_00012 13988 3 Skin 0.21 protein_coding synonymous_variant LOW 504A>C Ala168Ala
M0069966 MIKOKNHI_00012 13994 3 Skin 0.21 protein_coding synonymous_variant LOW 510C>G Ser170Ser
M0069967 MIKOKNHI_00012 14003 3 Skin 0.21 protein_coding synonymous_variant LOW 519C>T Ser173Ser
M0069968 MIKOKNHI_00012 14006 3 Skin 0.21 protein_coding synonymous_variant LOW 522C>A Gly174Gly
M0069969 MIKOKNHI_00012 14027 3 Skin 0.21 protein_coding synonymous_variant LOW 543C>T Arg181Arg
M0069970 MIKOKNHI_00012 14060 3 Skin 0.21 protein_coding synonymous_variant LOW 576G>T Ala192Ala
M0069971 MIKOKNHI_00012 14081 3 Skin 0.21 protein_coding synonymous_variant LOW 597C>T Gly199Gly
M0069972 MIKOKNHI_00012 14117 3 Skin 0.21 protein_coding synonymous_variant LOW 633T>C Arg211Arg
M0069973 MIKOKNHI_00012 14141 3 Skin 0.21 protein_coding synonymous_variant LOW 657C>T Asp219Asp
M0069974 MIKOKNHI_00012 14144 3 Skin 0.21 protein_coding synonymous_variant LOW 660T>C Tyr220Tyr
M0069975 MIKOKNHI_00012 14150 3 Skin 0.21 protein_coding synonymous_variant LOW 666C>T Gly222Gly
M0069976 MIKOKNHI_00002 3909 5 Skin 0.36 protein_coding upstream_gene_variant MODIFIER -1860C>G None
M0069977 MIKOKNHI_00002 2091 3 Skin 0.21 protein_coding upstream_gene_variant MODIFIER -42A>G None
M0069978 MIKOKNHI_00002 2149 3 Skin 0.21 protein_coding upstream_gene_variant MODIFIER -100G>A None
M0069979 MIKOKNHI_00003 2843 3 Skin 0.21 protein_coding missense_variant MODERATE 689C>T Thr230Ile






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
MIKOKNHI_00010 2.A.14.1.1 76.8 1.2e-241 1 552 1.0000 1.0000 2 Electrochemical Potential-driven Transporters 2.A Porters (uniporters, symporters, antiporters) 2.A.14 The Lactate Permease (LctP) Family