Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C233
  Reference Plasmid   HG796812.1
  Reference Plasmid Size   2773
  Reference Plasmid GC Content   0.51
  Reference Plasmid Mobility Type   mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0149303 MIEBFMPO_00001 73 4 Gut 0.50 protein_coding missense_variant MODERATE 50A>G Asn17Ser
M0149304 MIEBFMPO_00001 197 4 Gut 0.50 protein_coding synonymous_variant LOW 174G>A Lys58Lys
M0149305 MIEBFMPO_00001 200 4 Gut 0.50 protein_coding synonymous_variant LOW 177A>G Thr59Thr
M0149306 MIEBFMPO_00001 215 5 Gut 0.63 protein_coding synonymous_variant LOW 192C>T Phe64Phe
M0149307 MIEBFMPO_00001 290 5 Gut 0.63 protein_coding synonymous_variant LOW 267C>T Asn89Asn
M0149308 MIEBFMPO_00001 296 4 Gut 0.50 protein_coding synonymous_variant LOW 273C>T Leu91Leu
M0149309 MIEBFMPO_00001 398 4 Gut 0.50 protein_coding synonymous_variant LOW 375C>T Ala125Ala
M0149310 MIEBFMPO_00001 432 5 Gut 0.63 protein_coding synonymous_variant LOW 409T>C Leu137Leu
M0149311 MIEBFMPO_00001 453 5 Gut 0.63 protein_coding missense_variant MODERATE 430A>C Thr144Pro
M0149312 MIEBFMPO_00001 454 5 Gut 0.63 protein_coding missense_variant MODERATE 431C>T Thr144Ile
M0149313 MIEBFMPO_00001 500 4 Gut 0.50 protein_coding synonymous_variant LOW 477C>T Pro159Pro
M0149314 MIEBFMPO_00001 506 4 Gut 0.50 protein_coding synonymous_variant LOW 483A>G Val161Val
M0149315 MIEBFMPO_00001 515 4 Gut 0.50 protein_coding synonymous_variant LOW 492G>A Lys164Lys
M0149316 MIEBFMPO_00001 522 4 Gut 0.50 protein_coding synonymous_variant LOW 499C>T Leu167Leu
M0149317 MIEBFMPO_00001 561 5 Gut 0.63 protein_coding missense_variant MODERATE 538A>G Ile180Val
M0149318 MIEBFMPO_00001 599 4 Gut 0.50 protein_coding synonymous_variant LOW 576G>A Glu192Glu
M0149319 MIEBFMPO_00001 620 4 Gut 0.50 protein_coding synonymous_variant LOW 597C>A Leu199Leu
M0149320 MIEBFMPO_00001 632 5 Gut 0.63 protein_coding synonymous_variant LOW 609C>A Thr203Thr
M0149321 MIEBFMPO_00001 633 5 Gut 0.63 protein_coding missense_variant MODERATE 610T>C Tyr204His
M0149322 MIEBFMPO_00001 667 4 Gut 0.50 protein_coding missense_variant MODERATE 644C>T Ala215Val
M0149323 MIEBFMPO_00001 683 5 Gut 0.63 protein_coding missense_variant MODERATE 660A>G Ile220Met
M0149324 MIEBFMPO_00001 761 5 Gut 0.63 protein_coding synonymous_variant LOW 738G>A Lys246Lys
M0149325 MIEBFMPO_00001 785 4 Gut 0.50 protein_coding synonymous_variant LOW 762A>T Arg254Arg
M0149326 MIEBFMPO_00001 842 4 Gut 0.50 protein_coding synonymous_variant LOW 819A>G Gln273Gln
M0149327 MIEBFMPO_00001 893 5 Gut 0.63 protein_coding synonymous_variant LOW 870T>C Asn290Asn
M0149328 MIEBFMPO_00001 897 5 Gut 0.63 protein_coding missense_variant MODERATE 874G>A Gly292Ser
M0149329 MIEBFMPO_00001 931 4 Gut 0.50 protein_coding missense_variant MODERATE 908T>C Met303Thr
M0149330 MIEBFMPO_00001 946 4 Gut 0.50 protein_coding missense_variant MODERATE 923G>T Gly308Val
M0149331 MIEBFMPO_00001 1037 3 Gut 0.38 protein_coding synonymous_variant LOW 1014T>C Pro338Pro
M0149332 MIEBFMPO_00002 1405 6 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -99A>T None
M0149333 MIEBFMPO_00002 1441 6 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -63A>G None
M0149334 MIEBFMPO_00002 1452 6 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -52G>T None
M0149335 MIEBFMPO_00002 1554 4 Gut 0.50 protein_coding synonymous_variant LOW 51G>A Glu17Glu
M0149336 MIEBFMPO_00002 1587 5 Gut 0.63 protein_coding synonymous_variant LOW 84A>C Gly28Gly
M0149337 MIEBFMPO_00002 1594 5 Gut 0.63 protein_coding synonymous_variant LOW 91C>T Leu31Leu
M0149338 MIEBFMPO_00002 1599 4 Gut 0.50 protein_coding synonymous_variant LOW 96A>G Gln32Gln
M0149339 MIEBFMPO_00002 1602 4 Gut 0.50 protein_coding synonymous_variant LOW 99T>C Tyr33Tyr
M0149340 MIEBFMPO_00002 1611 4 Gut 0.50 protein_coding synonymous_variant LOW 108C>G Ala36Ala
M0149341 MIEBFMPO_00002 1641 5 Gut 0.63 protein_coding synonymous_variant LOW 138T>C Ser46Ser
M0149342 MIEBFMPO_00002 1704 5 Gut 0.63 protein_coding synonymous_variant LOW 201T>C Tyr67Tyr
M0149343 MIEBFMPO_00002 1971 4 Gut 0.50 protein_coding synonymous_variant LOW 468T>C Phe156Phe
M0149344 MIEBFMPO_00002 1998 5 Gut 0.63 protein_coding synonymous_variant LOW 495T>C Phe165Phe
M0149345 MIEBFMPO_00002 2019 5 Gut 0.63 protein_coding synonymous_variant LOW 516T>C Asp172Asp
M0149346 MIEBFMPO_00002 2146 3 Gut 0.38 protein_coding missense_variant MODERATE 643T>C Ser215Pro
M0149347 MIEBFMPO_00002 2205 4 Gut 0.50 protein_coding synonymous_variant LOW 702C>T Ser234Ser
M0149348 MIEBFMPO_00002 1192 5 Gut 0.63 protein_coding upstream_gene_variant MODIFIER -312A>T None
M0149349 MIEBFMPO_00002 1252 5 Gut 0.63 protein_coding upstream_gene_variant MODIFIER -252A>G None
M0149350 MIEBFMPO_00001 906 3 Gut 0.38 protein_coding missense_variant MODERATE 883A>G Ser295Gly
M0149351 MIEBFMPO_00001 2574 3 Gut 0.38 protein_coding downstream_gene_variant MODIFIER *1513C>T None
M0149352 MIEBFMPO_00001 2607 3 Gut 0.38 protein_coding downstream_gene_variant MODIFIER *1546C>T None
M0149353 MIEBFMPO_00001 2616 4 Gut 0.50 protein_coding downstream_gene_variant MODIFIER *1555A>T None
M0149354 MIEBFMPO_00001 2691 4 Gut 0.50 protein_coding downstream_gene_variant MODIFIER *1630A>C None
M0149355 MIEBFMPO_00001 2564 3 Gut 0.38 protein_coding downstream_gene_variant MODIFIER *1503G>C None
M0149356 MIEBFMPO_00001 2565 3 Gut 0.38 protein_coding downstream_gene_variant MODIFIER *1504C>T None






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term