Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C249
  Reference Plasmid   LR961983.1
  Reference Plasmid Size   30812
  Reference Plasmid GC Content   0.33
  Reference Plasmid Mobility Type   non-mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0149598 BMOAELGL_00013 9255 3 Gut 0.60 protein_coding synonymous_variant LOW 1269G>A Gly423Gly
M0149599 BMOAELGL_00013 9411 3 Gut 0.60 protein_coding synonymous_variant LOW 1425C>T Gly475Gly
M0149600 BMOAELGL_00013 9545 3 Gut 0.60 protein_coding missense_variant MODERATE 1559A>G Lys520Arg
M0149601 BMOAELGL_00013 9699 3 Gut 0.60 protein_coding synonymous_variant LOW 1713T>C Asn571Asn
M0149602 BMOAELGL_00013 9747 3 Gut 0.60 protein_coding synonymous_variant LOW 1761G>A Val587Val
M0149603 BMOAELGL_00013 9912 3 Gut 0.60 protein_coding synonymous_variant LOW 1926G>A Val642Val
M0149604 BMOAELGL_00013 9951 3 Gut 0.60 protein_coding synonymous_variant LOW 1965G>A Glu655Glu
M0149605 BMOAELGL_00013 9972 3 Gut 0.60 protein_coding synonymous_variant LOW 1986T>C Pro662Pro
M0149606 BMOAELGL_00013 9979 3 Gut 0.60 protein_coding synonymous_variant LOW 1993A>C Arg665Arg
M0149607 BMOAELGL_00013 9999 3 Gut 0.60 protein_coding synonymous_variant LOW 2013A>G Glu671Glu
M0149608 BMOAELGL_00013 10008 3 Gut 0.60 protein_coding synonymous_variant LOW 2022A>G Arg674Arg
M0149609 BMOAELGL_00013 10009 3 Gut 0.60 protein_coding synonymous_variant LOW 2023C>T Leu675Leu
M0149610 BMOAELGL_00013 10011 3 Gut 0.60 protein_coding synonymous_variant LOW 2025G>A Leu675Leu
M0149611 BMOAELGL_00013 10017 3 Gut 0.60 protein_coding synonymous_variant LOW 2031T>G Val677Val
M0149612 BMOAELGL_00013 10035 3 Gut 0.60 protein_coding synonymous_variant LOW 2049G>A Leu683Leu
M0149613 BMOAELGL_00013 10038 3 Gut 0.60 protein_coding synonymous_variant LOW 2052T>C Gly684Gly
M0149614 BMOAELGL_00013 10050 3 Gut 0.60 protein_coding synonymous_variant LOW 2064G>A Ser688Ser
M0149615 BMOAELGL_00013 10053 3 Gut 0.60 protein_coding synonymous_variant LOW 2067G>A Lys689Lys
M0149616 BMOAELGL_00013 10059 3 Gut 0.60 protein_coding synonymous_variant LOW 2073T>G Gly691Gly
M0149617 BMOAELGL_00013 10089 3 Gut 0.60 protein_coding synonymous_variant LOW 2103G>T Thr701Thr
M0149618 BMOAELGL_00014 10530 3 Gut 0.60 protein_coding synonymous_variant LOW 30A>C Ala10Ala
M0149619 BMOAELGL_00014 10539 3 Gut 0.60 protein_coding synonymous_variant LOW 39A>G Val13Val
M0149620 BMOAELGL_00014 10674 3 Gut 0.60 protein_coding synonymous_variant LOW 174C>T Val58Val
M0149621 BMOAELGL_00014 10863 3 Gut 0.60 protein_coding missense_variant MODERATE 363T>G Phe121Leu
M0149622 BMOAELGL_00014 10966 3 Gut 0.60 protein_coding missense_variant MODERATE 466A>G Ser156Gly
M0149623 BMOAELGL_00014 10989 3 Gut 0.60 protein_coding synonymous_variant LOW 489T>C Asn163Asn
M0149624 BMOAELGL_00014 10995 3 Gut 0.60 protein_coding synonymous_variant LOW 495T>A Gly165Gly
M0149625 BMOAELGL_00014 10998 3 Gut 0.60 protein_coding synonymous_variant LOW 498A>G Gly166Gly
M0149626 BMOAELGL_00014 11007 3 Gut 0.60 protein_coding synonymous_variant LOW 507T>C Ser169Ser
M0149627 BMOAELGL_00014 12330 4 Gut 0.80 protein_coding synonymous_variant LOW 1830G>A Gly610Gly
M0149628 BMOAELGL_00014 12338 3 Gut 0.60 protein_coding missense_variant MODERATE 1838C>T Ser613Leu
M0149629 BMOAELGL_00014 12339 3 Gut 0.60 protein_coding synonymous_variant LOW 1839A>G Ser613Ser
M0149630 BMOAELGL_00014 12421 3 Gut 0.60 protein_coding missense_variant MODERATE 1921A>G Asn641Asp
M0149631 BMOAELGL_00014 12438 3 Gut 0.60 protein_coding missense_variant MODERATE 1938T>A Asp646Glu
M0149632 BMOAELGL_00015 12637 3 Gut 0.60 protein_coding synonymous_variant LOW 64T>C Leu22Leu
M0149633 BMOAELGL_00015 12657 3 Gut 0.60 protein_coding synonymous_variant LOW 84G>T Val28Val
M0149634 BMOAELGL_00015 12720 3 Gut 0.60 protein_coding synonymous_variant LOW 147T>C Ser49Ser
M0149635 BMOAELGL_00015 12827 3 Gut 0.60 protein_coding missense_variant MODERATE 254G>A Gly85Glu
M0149636 BMOAELGL_00015 12927 4 Gut 0.80 protein_coding synonymous_variant LOW 354T>C Asp118Asp
M0149637 BMOAELGL_00015 13035 4 Gut 0.80 protein_coding synonymous_variant LOW 462G>A Gln154Gln
M0149638 BMOAELGL_00015 13050 3 Gut 0.60 protein_coding synonymous_variant LOW 477G>A Thr159Thr
M0149639 BMOAELGL_00015 13122 4 Gut 0.80 protein_coding synonymous_variant LOW 549A>C Gly183Gly
M0149640 BMOAELGL_00015 13125 4 Gut 0.80 protein_coding synonymous_variant LOW 552G>A Lys184Lys
M0149641 BMOAELGL_00015 13134 4 Gut 0.80 protein_coding synonymous_variant LOW 561T>G Val187Val
M0149642 BMOAELGL_00015 13140 4 Gut 0.80 protein_coding synonymous_variant LOW 567G>A Glu189Glu
M0149643 BMOAELGL_00015 13144 4 Gut 0.80 protein_coding missense_variant MODERATE 571G>A Val191Ile
M0149644 BMOAELGL_00015 13146 4 Gut 0.80 protein_coding synonymous_variant LOW 573A>G Val191Val
M0149645 BMOAELGL_00015 13161 4 Gut 0.80 protein_coding synonymous_variant LOW 588T>C Val196Val
M0149646 BMOAELGL_00015 13167 4 Gut 0.80 protein_coding synonymous_variant LOW 594T>A Gly198Gly
M0149647 BMOAELGL_00015 13176 3 Gut 0.60 protein_coding synonymous_variant LOW 603C>T Cys201Cys
M0149648 BMOAELGL_00015 13185 4 Gut 0.80 protein_coding synonymous_variant LOW 612T>G Gly204Gly
M0149649 BMOAELGL_00015 13191 4 Gut 0.80 protein_coding synonymous_variant LOW 618G>T Ser206Ser
M0149650 BMOAELGL_00015 13197 4 Gut 0.80 protein_coding synonymous_variant LOW 624C>T Tyr208Tyr
M0149651 BMOAELGL_00015 13200 4 Gut 0.80 protein_coding synonymous_variant LOW 627A>T Val209Val
M0149652 BMOAELGL_00015 13270 3 Gut 0.60 protein_coding missense_variant MODERATE 697T>G Ser233Ala
M0149653 BMOAELGL_00015 13287 3 Gut 0.60 protein_coding synonymous_variant LOW 714T>C Gly238Gly
M0149654 BMOAELGL_00015 13311 4 Gut 0.80 protein_coding synonymous_variant LOW 738T>C Asn246Asn
M0149655 BMOAELGL_00015 13347 3 Gut 0.60 protein_coding synonymous_variant LOW 774T>C Phe258Phe
M0149656 BMOAELGL_00015 13365 3 Gut 0.60 protein_coding synonymous_variant LOW 792A>G Ala264Ala
M0149657 BMOAELGL_00015 13377 3 Gut 0.60 protein_coding synonymous_variant LOW 804T>C Tyr268Tyr
M0149658 BMOAELGL_00016 13603 3 Gut 0.60 protein_coding synonymous_variant LOW 60T>C Gly20Gly
M0149659 BMOAELGL_00016 13615 3 Gut 0.60 protein_coding synonymous_variant LOW 72G>A Leu24Leu
M0149660 BMOAELGL_00016 13651 4 Gut 0.80 protein_coding missense_variant MODERATE 108T>A Asn36Lys
M0149661 BMOAELGL_00016 13660 4 Gut 0.80 protein_coding missense_variant MODERATE 117T>A Asp39Glu
M0149662 BMOAELGL_00016 13670 4 Gut 0.80 protein_coding missense_variant MODERATE 127G>C Glu43Gln
M0149663 BMOAELGL_00016 13697 3 Gut 0.60 protein_coding missense_variant MODERATE 154T>C Ser52Pro
M0149664 BMOAELGL_00016 13744 3 Gut 0.60 protein_coding synonymous_variant LOW 201C>T Asp67Asp
M0149665 BMOAELGL_00016 13755 3 Gut 0.60 protein_coding missense_variant MODERATE 212C>A Ser71Tyr
M0149666 BMOAELGL_00016 13803 4 Gut 0.80 protein_coding missense_variant MODERATE 260G>A Gly87Asp
M0149667 BMOAELGL_00016 13804 3 Gut 0.60 protein_coding synonymous_variant LOW 261T>A Gly87Gly
M0149668 BMOAELGL_00016 13825 4 Gut 0.80 protein_coding synonymous_variant LOW 282G>A Lys94Lys
M0149669 BMOAELGL_00017 13871 4 Gut 0.80 protein_coding missense_variant MODERATE 5T>C Leu2Ser
M0149670 BMOAELGL_00017 13879 4 Gut 0.80 protein_coding missense_variant MODERATE 13C>T Leu5Phe
M0149671 BMOAELGL_00017 13902 4 Gut 0.80 protein_coding synonymous_variant LOW 36G>A Thr12Thr
M0149672 BMOAELGL_00017 13994 4 Gut 0.80 protein_coding missense_variant MODERATE 128C>T Ala43Val
M0149673 BMOAELGL_00017 14025 4 Gut 0.80 protein_coding synonymous_variant LOW 159T>G Ser53Ser
M0149674 BMOAELGL_00018 14198 3 Gut 0.60 protein_coding synonymous_variant LOW 111A>G Thr37Thr
M0149675 BMOAELGL_00018 14220 3 Gut 0.60 protein_coding missense_variant MODERATE 133G>A Gly45Arg
M0149676 BMOAELGL_00018 14258 3 Gut 0.60 protein_coding synonymous_variant LOW 171A>T Thr57Thr
M0149677 BMOAELGL_00018 14284 3 Gut 0.60 protein_coding missense_variant MODERATE 197C>T Ser66Leu
M0149678 BMOAELGL_00018 14301 3 Gut 0.60 protein_coding missense_variant MODERATE 214A>G Ile72Val
M0149679 BMOAELGL_00018 14360 3 Gut 0.60 protein_coding synonymous_variant LOW 273T>C Arg91Arg
M0149680 BMOAELGL_00018 14370 3 Gut 0.60 protein_coding synonymous_variant LOW 283T>C Leu95Leu
M0149681 BMOAELGL_00018 14378 3 Gut 0.60 protein_coding synonymous_variant LOW 291T>C Pro97Pro
M0149682 BMOAELGL_00018 14391 3 Gut 0.60 protein_coding missense_variant MODERATE 304G>A Glu102Lys
M0149683 BMOAELGL_00018 14411 4 Gut 0.80 protein_coding synonymous_variant LOW 324G>A Gly108Gly
M0149684 BMOAELGL_00018 14447 4 Gut 0.80 protein_coding synonymous_variant LOW 360G>A Val120Val
M0149685 BMOAELGL_00018 14453 4 Gut 0.80 protein_coding synonymous_variant LOW 366A>G Lys122Lys
M0149686 BMOAELGL_00018 14481 4 Gut 0.80 protein_coding missense_variant MODERATE 394A>C Asn132His
M0149687 BMOAELGL_00018 14570 4 Gut 0.80 protein_coding synonymous_variant LOW 483A>T Ser161Ser
M0149688 BMOAELGL_00019 14611 3 Gut 0.60 protein_coding upstream_gene_variant MODIFIER -69C>T None
M0149689 BMOAELGL_00019 14621 3 Gut 0.60 protein_coding upstream_gene_variant MODIFIER -59T>A None
M0149690 BMOAELGL_00019 14626 3 Gut 0.60 protein_coding upstream_gene_variant MODIFIER -54C>A None
M0149691 BMOAELGL_00019 14633 3 Gut 0.60 protein_coding upstream_gene_variant MODIFIER -47A>G None
M0149692 BMOAELGL_00019 14634 3 Gut 0.60 protein_coding upstream_gene_variant MODIFIER -46T>C None
M0149693 BMOAELGL_00019 14636 3 Gut 0.60 protein_coding upstream_gene_variant MODIFIER -44G>A None
M0149694 BMOAELGL_00019 14638 3 Gut 0.60 protein_coding upstream_gene_variant MODIFIER -42G>A None
M0149695 BMOAELGL_00019 14648 3 Gut 0.60 protein_coding upstream_gene_variant MODIFIER -32T>C None
M0149696 BMOAELGL_00019 14654 3 Gut 0.60 protein_coding upstream_gene_variant MODIFIER -26G>C None
M0149697 BMOAELGL_00019 14656 3 Gut 0.60 protein_coding upstream_gene_variant MODIFIER -24T>C None
M0149698 BMOAELGL_00019 14700 3 Gut 0.60 protein_coding synonymous_variant LOW 21A>G Thr7Thr
M0149699 BMOAELGL_00019 14721 3 Gut 0.60 protein_coding synonymous_variant LOW 42A>G Gly14Gly
M0149700 BMOAELGL_00019 14724 3 Gut 0.60 protein_coding missense_variant MODERATE 45G>A Met15Ile
M0149701 BMOAELGL_00019 14737 3 Gut 0.60 protein_coding missense_variant MODERATE 58G>A Ala20Thr
M0149702 BMOAELGL_00019 14738 3 Gut 0.60 protein_coding missense_variant MODERATE 59C>G Ala20Gly
M0149703 BMOAELGL_00019 14739 3 Gut 0.60 protein_coding synonymous_variant LOW 60G>T Ala20Ala
M0149704 BMOAELGL_00019 14745 3 Gut 0.60 protein_coding synonymous_variant LOW 66T>G Gly22Gly






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism
BMOAELGL_00002 ARO:3002866 99.4 3.29e-121 5 166 0.9759 1.0000 diaminopyrimidine antibiotic trimethoprim resistant dihydrofolate reductase dfr antibiotic target replacement






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
BMOAELGL_00003 CCO10203.2|GH1 86.9 1.56e-244 1 390 1 1





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term