Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C250
  Reference Plasmid   LR962022.1
  Reference Plasmid Size   62226
  Reference Plasmid GC Content   0.35
  Reference Plasmid Mobility Type   mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0149705 AKBJGEAO_00047 37299 36 Gut 0.55 protein_coding missense_variant MODERATE 134T>G Ile45Arg
M0149706 AKBJGEAO_00051 39834 39 Gut 0.59 protein_coding synonymous_variant LOW 175C>T Leu59Leu
M0149707 AKBJGEAO_00063 50750 31 Gut 0.47 protein_coding missense_variant MODERATE 943T>C Tyr315His
M0149708 AKBJGEAO_00069 55056 17 Gut 0.26 protein_coding missense_variant MODERATE 1G>A Val1Met
M0149709 AKBJGEAO_00032 33307 4 Gut 0.06 protein_coding upstream_gene_variant MODIFIER -3659G>A None
M0149710 AKBJGEAO_00040 33883 6 Gut 0.09 protein_coding missense_variant MODERATE 133A>G Ile45Val
M0149711 AKBJGEAO_00040 33910 5 Gut 0.08 protein_coding synonymous_variant LOW 106C>T Leu36Leu
M0149712 AKBJGEAO_00040 33942 5 Gut 0.08 protein_coding missense_variant MODERATE 74T>C Val25Ala
M0149713 AKBJGEAO_00040 33967 4 Gut 0.06 protein_coding missense_variant MODERATE 49A>G Ile17Val
M0149714 AKBJGEAO_00041 34036 7 Gut 0.11 protein_coding stop_lost&splice_region_variant HIGH 172T>C Ter58Argext*?
M0149715 AKBJGEAO_00044 35641 3 Gut 0.05 protein_coding missense_variant MODERATE 5G>T Gly2Val
M0149716 AKBJGEAO_00054 42711 7 Gut 0.11 protein_coding missense_variant MODERATE 792T>A His264Gln
M0149717 AKBJGEAO_00058 46611 6 Gut 0.09 protein_coding upstream_gene_variant MODIFIER -220C>T None
M0149718 AKBJGEAO_00063 49994 11 Gut 0.17 protein_coding missense_variant MODERATE 187T>G Phe63Val
M0149719 AKBJGEAO_00064 52390 5 Gut 0.08 protein_coding missense_variant MODERATE 800A>G Lys267Arg
M0149720 AKBJGEAO_00068 54575 4 Gut 0.06 protein_coding stop_gained HIGH 145A>T Lys49*
M0149721 AKBJGEAO_00026 23353 3 Gut 0.05 protein_coding missense_variant MODERATE 988A>G Thr330Ala
M0149722 AKBJGEAO_00018 14676 3 Gut 0.05 protein_coding missense_variant MODERATE 485T>G Val162Gly
M0149723 AKBJGEAO_00053 41906 4 Gut 0.06 protein_coding missense_variant MODERATE 304A>G Arg102Gly
M0149724 AKBJGEAO_00047 37288 3 Gut 0.05 protein_coding missense_variant MODERATE 145A>G Asn49Asp
M0149725 AKBJGEAO_00057 45815 6 Gut 0.09 protein_coding synonymous_variant LOW 321C>T Asp107Asp
M0149726 AKBJGEAO_00060 47861 4 Gut 0.06 protein_coding missense_variant MODERATE 113T>C Ile38Thr
M0149727 AKBJGEAO_00062 48744 5 Gut 0.08 protein_coding missense_variant MODERATE 73A>G Met25Val
M0149728 AKBJGEAO_00040 33876 3 Gut 0.05 protein_coding missense_variant MODERATE 140A>C Asp47Ala
M0149729 AKBJGEAO_00043 35124 3 Gut 0.05 protein_coding missense_variant MODERATE 484A>G Thr162Ala
M0149730 AKBJGEAO_00043 35126 3 Gut 0.05 protein_coding synonymous_variant LOW 486A>G Thr162Thr
M0149731 AKBJGEAO_00043 35138 3 Gut 0.05 protein_coding synonymous_variant LOW 498C>T Val166Val
M0149732 AKBJGEAO_00043 35174 3 Gut 0.05 protein_coding synonymous_variant LOW 534C>T Asn178Asn
M0149733 AKBJGEAO_00043 35219 3 Gut 0.05 protein_coding synonymous_variant LOW 579G>A Gln193Gln
M0149734 AKBJGEAO_00051 40016 3 Gut 0.05 protein_coding synonymous_variant LOW 357C>T Asp119Asp
M0149735 AKBJGEAO_00051 40469 3 Gut 0.05 protein_coding synonymous_variant LOW 810C>T Asp270Asp






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition
AKBJGEAO_00064 VFG002162 BSH 73.8 1.9e-147 1 324 1.0 0.9969 Stress survival bile salt hydrolase experiment
AKBJGEAO_00064 VFG019385 BSH 74.4 3.3e-148 1 324 1.0 0.9969 Stress survival bile salt hydrolase prediction







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
AKBJGEAO_00055 QZK92746.1|GH1 100 0 1 468 1 1





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
AKBJGEAO_00054 4.A.3.1.1 71.9 1.1e-239 1 568 1.0000 0.9965 4 Group Translocators 4.A Phosphotransfer-driven Group Translocators (PTS) 4.A.3 The PTS Lactose-N,N'-Diacetylchitobiose-β-glucoside (Lac) Family