Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C251
  Reference Plasmid   LR962118.1
  Reference Plasmid Size   54470
  Reference Plasmid GC Content   0.35
  Reference Plasmid Mobility Type   non-mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0149736 MBMONOOD_00017 18793 4 Gut 0.80 protein_coding synonymous_variant LOW 375A>G Leu125Leu
M0149737 MBMONOOD_00017 18961 4 Gut 0.80 protein_coding synonymous_variant LOW 543A>T Pro181Pro
M0149738 MBMONOOD_00017 19195 4 Gut 0.80 protein_coding synonymous_variant LOW 777C>T Asn259Asn
M0149739 MBMONOOD_00017 19486 4 Gut 0.80 protein_coding synonymous_variant LOW 1068T>C Asn356Asn
M0149740 MBMONOOD_00017 19714 4 Gut 0.80 protein_coding missense_variant MODERATE 1296T>G Asp432Glu
M0149741 MBMONOOD_00019 21219 4 Gut 0.80 protein_coding missense_variant MODERATE 175C>A Leu59Ile
M0149742 MBMONOOD_00019 21222 4 Gut 0.80 protein_coding missense_variant MODERATE 178A>G Ser60Gly
M0149743 MBMONOOD_00019 21245 4 Gut 0.80 protein_coding synonymous_variant LOW 201T>C Cys67Cys
M0149744 MBMONOOD_00019 21374 4 Gut 0.80 protein_coding synonymous_variant LOW 330C>T Asp110Asp
M0149745 MBMONOOD_00020 23383 3 Gut 0.60 protein_coding missense_variant MODERATE 118A>G Ile40Val
M0149746 MBMONOOD_00020 23433 3 Gut 0.60 protein_coding synonymous_variant LOW 168T>C Tyr56Tyr
M0149747 MBMONOOD_00020 23490 3 Gut 0.60 protein_coding synonymous_variant LOW 225C>A Ala75Ala
M0149748 MBMONOOD_00020 24402 3 Gut 0.60 protein_coding synonymous_variant LOW 1137G>A Lys379Lys
M0149749 MBMONOOD_00020 24676 3 Gut 0.60 protein_coding missense_variant MODERATE 1411A>G Ile471Val
M0149750 MBMONOOD_00020 24695 3 Gut 0.60 protein_coding missense_variant MODERATE 1430T>C Ile477Thr
M0149751 MBMONOOD_00020 24711 3 Gut 0.60 protein_coding synonymous_variant LOW 1446T>A Pro482Pro
M0149752 MBMONOOD_00020 25125 3 Gut 0.60 protein_coding synonymous_variant LOW 1860C>T Ser620Ser
M0149753 MBMONOOD_00021 25370 3 Gut 0.60 protein_coding synonymous_variant LOW 201G>A Val67Val
M0149754 MBMONOOD_00025 28900 3 Gut 0.60 protein_coding missense_variant MODERATE 582T>G Asp194Glu
M0149755 MBMONOOD_00025 28969 3 Gut 0.60 protein_coding synonymous_variant LOW 651T>C Ala217Ala
M0149756 MBMONOOD_00025 29113 3 Gut 0.60 protein_coding synonymous_variant LOW 795G>A Val265Val
M0149757 MBMONOOD_00025 29182 3 Gut 0.60 protein_coding synonymous_variant LOW 864C>T Gly288Gly
M0149758 MBMONOOD_00025 29422 3 Gut 0.60 protein_coding synonymous_variant LOW 1104G>A Arg368Arg
M0149759 MBMONOOD_00026 29673 3 Gut 0.60 protein_coding synonymous_variant LOW 147G>A Ser49Ser
M0149760 MBMONOOD_00026 29880 3 Gut 0.60 protein_coding synonymous_variant LOW 354T>C Ile118Ile
M0149761 MBMONOOD_00027 30272 3 Gut 0.60 protein_coding synonymous_variant LOW 219T>C Asp73Asp
M0149762 MBMONOOD_00027 30590 3 Gut 0.60 protein_coding synonymous_variant LOW 537G>A Gln179Gln
M0149763 MBMONOOD_00027 30727 3 Gut 0.60 protein_coding missense_variant MODERATE 674T>A Val225Glu
M0149764 MBMONOOD_00027 30746 3 Gut 0.60 protein_coding synonymous_variant LOW 693C>T Ser231Ser
M0149765 MBMONOOD_00028 31017 3 Gut 0.60 protein_coding upstream_gene_variant MODIFIER -283C>A None
M0149766 MBMONOOD_00028 31122 3 Gut 0.60 protein_coding upstream_gene_variant MODIFIER -178T>C None
M0149767 MBMONOOD_00028 31491 3 Gut 0.60 protein_coding synonymous_variant LOW 192G>A Thr64Thr
M0149768 MBMONOOD_00029 31663 3 Gut 0.60 protein_coding synonymous_variant LOW 111A>G Leu37Leu
M0149769 MBMONOOD_00029 32092 3 Gut 0.60 protein_coding synonymous_variant LOW 540T>C Ile180Ile
M0149770 MBMONOOD_00030 32512 3 Gut 0.60 protein_coding synonymous_variant LOW 400T>C Leu134Leu
M0149771 MBMONOOD_00030 32652 3 Gut 0.60 protein_coding synonymous_variant LOW 540A>C Gly180Gly
M0149772 MBMONOOD_00030 33142 3 Gut 0.60 protein_coding synonymous_variant LOW 1030T>C Leu344Leu
M0149773 MBMONOOD_00033 34009 3 Gut 0.60 protein_coding synonymous_variant LOW 39C>A Ala13Ala
M0149774 MBMONOOD_00033 34069 3 Gut 0.60 protein_coding synonymous_variant LOW 99A>G Arg33Arg
M0149775 MBMONOOD_00033 34153 3 Gut 0.60 protein_coding synonymous_variant LOW 183T>C Asp61Asp
M0149776 MBMONOOD_00033 34339 3 Gut 0.60 protein_coding synonymous_variant LOW 369G>A Arg123Arg
M0149777 MBMONOOD_00033 34393 3 Gut 0.60 protein_coding synonymous_variant LOW 423T>C His141His
M0149778 MBMONOOD_00033 34402 3 Gut 0.60 protein_coding synonymous_variant LOW 432G>A Gly144Gly
M0149779 MBMONOOD_00033 34612 3 Gut 0.60 protein_coding synonymous_variant LOW 642G>A Pro214Pro
M0149780 MBMONOOD_00033 34840 3 Gut 0.60 protein_coding synonymous_variant LOW 870G>A Arg290Arg
M0149781 MBMONOOD_00033 34954 3 Gut 0.60 protein_coding synonymous_variant LOW 984A>G Ala328Ala
M0149782 MBMONOOD_00033 35014 3 Gut 0.60 protein_coding synonymous_variant LOW 1044C>T Val348Val
M0149783 MBMONOOD_00033 35068 3 Gut 0.60 protein_coding synonymous_variant LOW 1098T>C Val366Val
M0149784 MBMONOOD_00033 35188 3 Gut 0.60 protein_coding synonymous_variant LOW 1218T>G Thr406Thr
M0149785 MBMONOOD_00033 35221 3 Gut 0.60 protein_coding synonymous_variant LOW 1251G>A Arg417Arg
M0149786 MBMONOOD_00033 35443 3 Gut 0.60 protein_coding synonymous_variant LOW 1473T>C Leu491Leu
M0149787 MBMONOOD_00033 35487 3 Gut 0.60 protein_coding missense_variant MODERATE 1517A>T Gln506Leu
M0149788 MBMONOOD_00033 35528 3 Gut 0.60 protein_coding missense_variant MODERATE 1558A>T Thr520Ser
M0149789 MBMONOOD_00034 35879 3 Gut 0.60 protein_coding synonymous_variant LOW 321A>T Val107Val
M0149790 MBMONOOD_00034 35933 3 Gut 0.60 protein_coding synonymous_variant LOW 375A>G Leu125Leu
M0149791 MBMONOOD_00034 36057 3 Gut 0.60 protein_coding missense_variant MODERATE 499C>A Gln167Lys
M0149792 MBMONOOD_00034 36099 3 Gut 0.60 protein_coding missense_variant MODERATE 541A>G Thr181Ala
M0149793 MBMONOOD_00034 36525 3 Gut 0.60 protein_coding missense_variant MODERATE 967A>G Thr323Ala
M0149794 MBMONOOD_00034 36591 3 Gut 0.60 protein_coding missense_variant MODERATE 1033A>G Lys345Glu
M0149795 MBMONOOD_00034 36601 3 Gut 0.60 protein_coding missense_variant MODERATE 1043G>A Gly348Asp
M0149796 MBMONOOD_00034 36830 3 Gut 0.60 protein_coding synonymous_variant LOW 1272G>A Pro424Pro
M0149797 MBMONOOD_00034 36950 3 Gut 0.60 protein_coding synonymous_variant LOW 1392C>T Tyr464Tyr
M0149798 MBMONOOD_00034 37351 3 Gut 0.60 protein_coding missense_variant MODERATE 1793C>T Ser598Phe
M0149799 MBMONOOD_00034 37537 3 Gut 0.60 protein_coding missense_variant MODERATE 1979A>C Asn660Thr
M0149800 MBMONOOD_00034 37745 3 Gut 0.60 protein_coding synonymous_variant LOW 2187G>A Lys729Lys
M0149801 MBMONOOD_00034 38160 3 Gut 0.60 protein_coding synonymous_variant LOW 2602C>T Leu868Leu
M0149802 MBMONOOD_00034 38351 3 Gut 0.60 protein_coding synonymous_variant LOW 2793G>T Gly931Gly
M0149803 MBMONOOD_00034 38378 3 Gut 0.60 protein_coding synonymous_variant LOW 2820C>T Asn940Asn
M0149804 MBMONOOD_00034 38789 3 Gut 0.60 protein_coding synonymous_variant LOW 3231A>G Ser1077Ser
M0149805 MBMONOOD_00034 38967 3 Gut 0.60 protein_coding synonymous_variant LOW 3409C>A Arg1137Arg
M0149806 MBMONOOD_00034 39170 3 Gut 0.60 protein_coding synonymous_variant LOW 3612G>A Lys1204Lys
M0149807 MBMONOOD_00034 39174 3 Gut 0.60 protein_coding missense_variant MODERATE 3616T>G Ser1206Ala
M0149808 MBMONOOD_00034 41399 4 Gut 0.80 protein_coding synonymous_variant LOW 5841G>A Gln1947Gln
M0149809 MBMONOOD_00034 41734 4 Gut 0.80 protein_coding missense_variant MODERATE 6176A>G Gln2059Arg
M0149810 MBMONOOD_00034 41735 4 Gut 0.80 protein_coding missense_variant MODERATE 6177A>T Gln2059His
M0149811 MBMONOOD_00034 41922 4 Gut 0.80 protein_coding missense_variant MODERATE 6364T>G Ser2122Ala
M0149812 MBMONOOD_00034 41957 4 Gut 0.80 protein_coding synonymous_variant LOW 6399T>C Val2133Val
M0149813 MBMONOOD_00034 42258 4 Gut 0.80 protein_coding missense_variant MODERATE 6700A>G Ile2234Val
M0149814 MBMONOOD_00035 42476 4 Gut 0.80 protein_coding synonymous_variant LOW 63G>A Lys21Lys
M0149815 MBMONOOD_00036 42930 4 Gut 0.80 protein_coding synonymous_variant LOW 237G>A Gly79Gly
M0149816 MBMONOOD_00037 43184 4 Gut 0.80 protein_coding synonymous_variant LOW 84G>A Leu28Leu
M0149817 MBMONOOD_00037 43242 4 Gut 0.80 protein_coding missense_variant MODERATE 142G>C Val48Leu
M0149818 MBMONOOD_00037 43296 4 Gut 0.80 protein_coding synonymous_variant LOW 196C>T Leu66Leu
M0149819 MBMONOOD_00037 43304 4 Gut 0.80 protein_coding synonymous_variant LOW 204A>C Ala68Ala
M0149820 MBMONOOD_00037 43364 4 Gut 0.80 protein_coding synonymous_variant LOW 264A>G Glu88Glu
M0149821 MBMONOOD_00038 43438 4 Gut 0.80 protein_coding upstream_gene_variant MODIFIER -11C>T None
M0149822 MBMONOOD_00038 43537 4 Gut 0.80 protein_coding missense_variant MODERATE 89T>C Met30Thr
M0149823 MBMONOOD_00038 43560 4 Gut 0.80 protein_coding missense_variant MODERATE 112A>C Ile38Leu
M0149824 MBMONOOD_00038 43586 4 Gut 0.80 protein_coding synonymous_variant LOW 138A>G Leu46Leu
M0149825 MBMONOOD_00038 43712 4 Gut 0.80 protein_coding synonymous_variant LOW 264C>T Tyr88Tyr
M0149826 MBMONOOD_00040 44185 4 Gut 0.80 protein_coding synonymous_variant LOW 165C>T Arg55Arg
M0149827 MBMONOOD_00040 44299 4 Gut 0.80 protein_coding synonymous_variant LOW 279G>A Thr93Thr
M0149828 MBMONOOD_00042 44972 4 Gut 0.80 protein_coding missense_variant MODERATE 31T>G Leu11Val
M0149829 MBMONOOD_00042 44981 4 Gut 0.80 protein_coding synonymous_variant LOW 40C>T Leu14Leu
M0149830 MBMONOOD_00017 19510 3 Gut 0.60 protein_coding missense_variant MODERATE 1092T>A Asn364Lys
M0149831 MBMONOOD_00017 19747 3 Gut 0.60 protein_coding synonymous_variant LOW 1329G>A Ala443Ala
M0149832 MBMONOOD_00017 19762 3 Gut 0.60 protein_coding missense_variant MODERATE 1344G>T Leu448Phe
M0149833 MBMONOOD_00017 19802 3 Gut 0.60 protein_coding missense_variant MODERATE 1384A>G Thr462Ala
M0149834 MBMONOOD_00019 20826 3 Gut 0.60 protein_coding upstream_gene_variant MODIFIER -219T>G None
M0149835 MBMONOOD_00019 21131 3 Gut 0.60 protein_coding synonymous_variant LOW 87A>T Gly29Gly
M0149836 MBMONOOD_00019 21454 3 Gut 0.60 protein_coding missense_variant MODERATE 410C>T Thr137Ile
M0149837 MBMONOOD_00019 21515 3 Gut 0.60 protein_coding synonymous_variant LOW 471C>T Thr157Thr
M0149838 MBMONOOD_00019 21615 3 Gut 0.60 protein_coding missense_variant MODERATE 571C>A Leu191Ile
M0149839 MBMONOOD_00019 21839 3 Gut 0.60 protein_coding synonymous_variant LOW 795A>G Glu265Glu
M0149840 MBMONOOD_00019 21965 3 Gut 0.60 protein_coding synonymous_variant LOW 921A>G Glu307Glu
M0149841 MBMONOOD_00019 21974 3 Gut 0.60 protein_coding synonymous_variant LOW 930G>A Ala310Ala
M0149842 MBMONOOD_00019 22154 3 Gut 0.60 protein_coding synonymous_variant LOW 1110G>A Leu370Leu
M0149843 MBMONOOD_00019 22328 3 Gut 0.60 protein_coding synonymous_variant LOW 1284A>G Lys428Lys
M0149844 MBMONOOD_00019 22358 3 Gut 0.60 protein_coding synonymous_variant LOW 1314G>A Ala438Ala
M0149845 MBMONOOD_00034 41843 3 Gut 0.60 protein_coding synonymous_variant LOW 6285C>T Ala2095Ala
M0149846 MBMONOOD_00034 42162 3 Gut 0.60 protein_coding missense_variant MODERATE 6604G>A Val2202Met
M0149847 MBMONOOD_00036 43064 3 Gut 0.60 protein_coding missense_variant MODERATE 371C>T Ala124Val
M0149848 MBMONOOD_00040 44245 3 Gut 0.60 protein_coding synonymous_variant LOW 225T>G Gly75Gly
M0149849 MBMONOOD_00040 44410 3 Gut 0.60 protein_coding synonymous_variant LOW 390G>A Gln130Gln
M0149850 MBMONOOD_00041 44551 3 Gut 0.60 protein_coding missense_variant MODERATE 25T>A Ser9Thr
M0149851 MBMONOOD_00041 44649 3 Gut 0.60 protein_coding missense_variant MODERATE 123T>A Phe41Leu
M0149852 MBMONOOD_00041 44670 3 Gut 0.60 protein_coding synonymous_variant LOW 144C>T Pro48Pro
M0149853 MBMONOOD_00041 44796 3 Gut 0.60 protein_coding synonymous_variant LOW 270A>G Thr90Thr
M0149854 MBMONOOD_00041 44945 3 Gut 0.60 protein_coding missense_variant MODERATE 419A>G Glu140Gly
M0149855 MBMONOOD_00042 45091 3 Gut 0.60 protein_coding synonymous_variant LOW 150A>C Ala50Ala
M0149856 MBMONOOD_00042 45256 3 Gut 0.60 protein_coding missense_variant MODERATE 315G>C Lys105Asn
M0149857 MBMONOOD_00043 45332 3 Gut 0.60 protein_coding upstream_gene_variant MODIFIER -105G>T None
M0149858 MBMONOOD_00043 45755 3 Gut 0.60 protein_coding missense_variant MODERATE 319G>A Val107Ile
M0149859 MBMONOOD_00043 45860 3 Gut 0.60 protein_coding synonymous_variant LOW 424T>C Leu142Leu
M0149860 MBMONOOD_00043 46073 3 Gut 0.60 protein_coding missense_variant MODERATE 637C>T Leu213Phe
M0149861 MBMONOOD_00043 46103 3 Gut 0.60 protein_coding missense_variant MODERATE 667A>G Thr223Ala
M0149862 MBMONOOD_00043 46108 3 Gut 0.60 protein_coding synonymous_variant LOW 672T>A Ala224Ala
M0149863 MBMONOOD_00043 46183 3 Gut 0.60 protein_coding synonymous_variant LOW 747G>A Gly249Gly
M0149864 MBMONOOD_00043 46192 3 Gut 0.60 protein_coding synonymous_variant LOW 756C>A Ser252Ser
M0149865 MBMONOOD_00043 46441 3 Gut 0.60 protein_coding synonymous_variant LOW 1005G>T Gly335Gly
M0149866 MBMONOOD_00043 46477 3 Gut 0.60 protein_coding synonymous_variant LOW 1041A>G Leu347Leu
M0149867 MBMONOOD_00043 46478 3 Gut 0.60 protein_coding missense_variant MODERATE 1042T>G Ser348Ala
M0149868 MBMONOOD_00043 46485 3 Gut 0.60 protein_coding missense_variant MODERATE 1049T>C Ile350Thr
M0149869 MBMONOOD_00043 46516 3 Gut 0.60 protein_coding synonymous_variant LOW 1080T>C Arg360Arg
M0149870 MBMONOOD_00043 46564 3 Gut 0.60 protein_coding synonymous_variant LOW 1128T>A Thr376Thr
M0149871 MBMONOOD_00043 46655 3 Gut 0.60 protein_coding synonymous_variant LOW 1219C>T Leu407Leu
M0149872 MBMONOOD_00043 46659 3 Gut 0.60 protein_coding missense_variant MODERATE 1223T>G Phe408Cys
M0149873 MBMONOOD_00043 46660 3 Gut 0.60 protein_coding synonymous_variant LOW 1224C>T Phe408Phe
M0149874 MBMONOOD_00043 46670 3 Gut 0.60 protein_coding synonymous_variant LOW 1234C>T Leu412Leu
M0149875 MBMONOOD_00043 46678 3 Gut 0.60 protein_coding synonymous_variant LOW 1242G>A Leu414Leu
M0149876 MBMONOOD_00043 46697 3 Gut 0.60 protein_coding missense_variant MODERATE 1261A>G Met421Val
M0149877 MBMONOOD_00045 46721 3 Gut 0.60 protein_coding upstream_gene_variant MODIFIER -1666G>A None
M0149878 MBMONOOD_00045 46831 3 Gut 0.60 protein_coding upstream_gene_variant MODIFIER -1556T>C None






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition
MBMONOOD_00010 VFG002173 AS 98.1 0 1 1296 1.0 1 Adherence aggregation substance Asa1 experiment
MBMONOOD_00010 VFG002173 AS 98.1 0 1 1296 1.0 1 Adherence aggregation substance Asa1 prediction







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
MBMONOOD_00025 CBL31237.1|GH73 99.5 6.49e-286 1 392 1 1
MBMONOOD_00043 AAL59475.1|GT0 97.8 9.37e-207 112 426 0.7394 1





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term