Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C253
  Reference Plasmid   LR962172.1
  Reference Plasmid Size   94567
  Reference Plasmid GC Content   0.33
  Reference Plasmid Mobility Type   non-mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0149881 KOLACBJI_00050 49144 3 Gut 0.30 protein_coding synonymous_variant LOW 451C>T Leu151Leu
M0149882 KOLACBJI_00051 49816 6 Gut 0.60 protein_coding synonymous_variant LOW 363C>T Val121Val
M0149883 KOLACBJI_00051 50003 8 Gut 0.80 protein_coding missense_variant MODERATE 550A>G Ile184Val
M0149884 KOLACBJI_00051 50681 3 Gut 0.30 protein_coding missense_variant MODERATE 1228G>A Ala410Thr
M0149885 KOLACBJI_00051 49696 3 Gut 0.30 protein_coding synonymous_variant LOW 243T>C Asn81Asn
M0149886 KOLACBJI_00051 49826 3 Gut 0.30 protein_coding missense_variant MODERATE 373G>A Val125Ile
M0149887 KOLACBJI_00051 50857 3 Gut 0.30 protein_coding synonymous_variant LOW 1404T>C Thr468Thr
M0149888 KOLACBJI_00049 51575 3 Gut 0.30 protein_coding upstream_gene_variant MODIFIER -2938G>A None






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition
KOLACBJI_00003 VFG002173 AS 97.2 0 1 1295 1.0 0.9992 Adherence aggregation substance Asa1 experiment
KOLACBJI_00003 VFG002173 AS 97.2 0 1 1295 1.0 0.9992 Adherence aggregation substance Asa1 prediction







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism
KOLACBJI_00031 ARO:3000375 78.4 4.21e-137 1 245 1.0000 0.9879 macrolide antibiotic Erm 23S ribosomal RNA methyltransferase antibiotic target alteration






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
KOLACBJI_00039 AAL59475.1|GT0 96.2 4.05e-205 128 442 0.7127 1





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
KOLACBJI_00021 3.A.7.19.1 97.5 0 1 605 0.9967 1.0784 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.7 The Type IV (Conjugal DNA-Protein Transfer or VirB) Secretory Pathway (IVSP) Family
KOLACBJI_00024 3.A.7.19.1 98.7 0 1 719 1.0000 1.2816 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.7 The Type IV (Conjugal DNA-Protein Transfer or VirB) Secretory Pathway (IVSP) Family
KOLACBJI_00027 3.A.7.19.1 71.2 2.3e-224 4 564 0.9947 0.9750 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.7 The Type IV (Conjugal DNA-Protein Transfer or VirB) Secretory Pathway (IVSP) Family
KOLACBJI_00032 3.A.7.19.1 86.1 2.5e-226 1 444 1.0000 0.7932 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.7 The Type IV (Conjugal DNA-Protein Transfer or VirB) Secretory Pathway (IVSP) Family