Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C3131
  Reference Plasmid   MET0225_bin.16__k81_14946
  Reference Plasmid Size   21088
  Reference Plasmid GC Content   0.71
  Reference Plasmid Mobility Type   non-mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0076163 KHGDHFHA_00015 13762 5 Skin 0.28 protein_coding synonymous_variant LOW 243T>C His81His
M0076164 KHGDHFHA_00015 13777 5 Skin 0.28 protein_coding synonymous_variant LOW 258C>G Leu86Leu
M0076165 KHGDHFHA_00015 13804 4 Skin 0.22 protein_coding synonymous_variant LOW 285T>G Ala95Ala
M0076166 KHGDHFHA_00015 13807 5 Skin 0.28 protein_coding synonymous_variant LOW 288C>G Val96Val
M0076167 KHGDHFHA_00015 13810 4 Skin 0.22 protein_coding synonymous_variant LOW 291T>C Asp97Asp
M0076168 KHGDHFHA_00015 13826 5 Skin 0.28 protein_coding missense_variant MODERATE 307G>A Val103Met
M0076169 KHGDHFHA_00015 13828 3 Skin 0.17 protein_coding synonymous_variant LOW 309G>A Val103Val
M0076170 KHGDHFHA_00015 13832 5 Skin 0.28 protein_coding missense_variant MODERATE 313T>G Ser105Ala
M0076171 KHGDHFHA_00016 14097 5 Skin 0.28 protein_coding synonymous_variant LOW 261A>G Thr87Thr
M0076172 KHGDHFHA_00016 14274 5 Skin 0.28 protein_coding synonymous_variant LOW 438C>G Leu146Leu
M0076173 KHGDHFHA_00016 14619 5 Skin 0.28 protein_coding synonymous_variant LOW 783G>C Arg261Arg
M0076174 KHGDHFHA_00016 15270 3 Skin 0.17 protein_coding synonymous_variant LOW 1434C>T Pro478Pro
M0076175 KHGDHFHA_00016 15273 3 Skin 0.17 protein_coding synonymous_variant LOW 1437G>A Glu479Glu
M0076176 KHGDHFHA_00016 15276 3 Skin 0.17 protein_coding synonymous_variant LOW 1440C>G Val480Val
M0076177 KHGDHFHA_00016 15282 4 Skin 0.22 protein_coding synonymous_variant LOW 1446T>C Arg482Arg
M0076178 KHGDHFHA_00016 15291 4 Skin 0.22 protein_coding synonymous_variant LOW 1455C>G Ala485Ala
M0076179 KHGDHFHA_00016 15292 4 Skin 0.22 protein_coding missense_variant MODERATE 1456G>A Ala486Thr
M0076180 KHGDHFHA_00016 15294 4 Skin 0.22 protein_coding synonymous_variant LOW 1458G>C Ala486Ala
M0076181 KHGDHFHA_00016 15617 4 Skin 0.22 protein_coding missense_variant MODERATE 1781G>A Arg594His
M0076182 KHGDHFHA_00017 15836 5 Skin 0.28 protein_coding synonymous_variant LOW 240G>A Glu80Glu
M0076183 KHGDHFHA_00017 15841 5 Skin 0.28 protein_coding missense_variant MODERATE 235G>C Gly79Arg
M0076184 KHGDHFHA_00017 15842 5 Skin 0.28 protein_coding synonymous_variant LOW 234T>C Arg78Arg
M0076185 KHGDHFHA_00017 16025 5 Skin 0.28 protein_coding synonymous_variant LOW 51A>G Gly17Gly
M0076186 KHGDHFHA_00017 16028 5 Skin 0.28 protein_coding synonymous_variant LOW 48C>T Pro16Pro
M0076187 KHGDHFHA_00017 16029 5 Skin 0.28 protein_coding missense_variant MODERATE 47C>T Pro16Leu
M0076188 KHGDHFHA_00019 16581 5 Skin 0.28 protein_coding missense_variant MODERATE 1355T>C Met452Thr
M0076189 KHGDHFHA_00019 16865 7 Skin 0.39 protein_coding synonymous_variant LOW 1071G>A Thr357Thr
M0076190 KHGDHFHA_00019 17213 4 Skin 0.22 protein_coding synonymous_variant LOW 723T>C Val241Val
M0076191 KHGDHFHA_00019 17215 4 Skin 0.22 protein_coding missense_variant MODERATE 721G>A Val241Ile
M0076192 KHGDHFHA_00019 17216 4 Skin 0.22 protein_coding synonymous_variant LOW 720T>C Ala240Ala
M0076193 KHGDHFHA_00019 17228 4 Skin 0.22 protein_coding synonymous_variant LOW 708T>G Val236Val
M0076194 KHGDHFHA_00019 17231 4 Skin 0.22 protein_coding synonymous_variant LOW 705G>A Arg235Arg
M0076195 KHGDHFHA_00019 17246 4 Skin 0.22 protein_coding synonymous_variant LOW 690G>C Ala230Ala
M0076196 KHGDHFHA_00019 17252 4 Skin 0.22 protein_coding synonymous_variant LOW 684T>C Val228Val
M0076197 KHGDHFHA_00019 17254 4 Skin 0.22 protein_coding missense_variant MODERATE 682G>A Val228Ile
M0076198 KHGDHFHA_00019 17256 4 Skin 0.22 protein_coding missense_variant MODERATE 680G>A Gly227Glu
M0076199 KHGDHFHA_00019 17267 4 Skin 0.22 protein_coding synonymous_variant LOW 669A>G Lys223Lys
M0076200 KHGDHFHA_00019 17276 4 Skin 0.22 protein_coding synonymous_variant LOW 660G>A Glu220Glu
M0076201 KHGDHFHA_00019 17308 5 Skin 0.28 protein_coding synonymous_variant LOW 628C>A Arg210Arg
M0076202 KHGDHFHA_00019 17432 5 Skin 0.28 protein_coding synonymous_variant LOW 504G>C Thr168Thr
M0076203 KHGDHFHA_00019 17438 5 Skin 0.28 protein_coding synonymous_variant LOW 498C>T Tyr166Tyr
M0076204 KHGDHFHA_00019 17439 5 Skin 0.28 protein_coding missense_variant MODERATE 497A>T Tyr166Phe
M0076205 KHGDHFHA_00019 17442 5 Skin 0.28 protein_coding missense_variant MODERATE 494G>A Gly165Asp
M0076206 KHGDHFHA_00019 17456 5 Skin 0.28 protein_coding synonymous_variant LOW 480A>G Gly160Gly
M0076207 KHGDHFHA_00019 17466 5 Skin 0.28 protein_coding missense_variant MODERATE 470T>C Leu157Pro
M0076208 KHGDHFHA_00019 17467 5 Skin 0.28 protein_coding missense_variant MODERATE 469C>G Leu157Val
M0076209 KHGDHFHA_00019 17468 5 Skin 0.28 protein_coding synonymous_variant LOW 468G>T Pro156Pro
M0076210 KHGDHFHA_00019 17489 5 Skin 0.28 protein_coding synonymous_variant LOW 447C>A Thr149Thr
M0076211 KHGDHFHA_00019 17497 5 Skin 0.28 protein_coding missense_variant MODERATE 439G>A Val147Ile
M0076212 KHGDHFHA_00019 17518 4 Skin 0.22 protein_coding missense_variant MODERATE 418G>A Val140Ile
M0076213 KHGDHFHA_00019 17554 4 Skin 0.22 protein_coding missense_variant MODERATE 382G>C Val128Leu
M0076214 KHGDHFHA_00019 17560 4 Skin 0.22 protein_coding missense_variant MODERATE 376C>T Pro126Ser
M0076215 KHGDHFHA_00019 17570 4 Skin 0.22 protein_coding synonymous_variant LOW 366C>G Thr122Thr
M0076216 KHGDHFHA_00019 17573 4 Skin 0.22 protein_coding synonymous_variant LOW 363A>C Gly121Gly
M0076217 KHGDHFHA_00019 17618 4 Skin 0.22 protein_coding synonymous_variant LOW 318G>A Glu106Glu
M0076218 KHGDHFHA_00019 17621 4 Skin 0.22 protein_coding synonymous_variant LOW 315C>A Ala105Ala
M0076219 KHGDHFHA_00019 17627 4 Skin 0.22 protein_coding synonymous_variant LOW 309T>C Asp103Asp
M0076220 KHGDHFHA_00020 18379 4 Skin 0.22 protein_coding missense_variant MODERATE 329T>A Val110Asp
M0076221 KHGDHFHA_00021 18734 4 Skin 0.22 protein_coding synonymous_variant LOW 1017G>C Gly339Gly
M0076222 KHGDHFHA_00021 18855 4 Skin 0.22 protein_coding missense_variant MODERATE 896T>C Leu299Pro
M0076223 KHGDHFHA_00008 8834 3 Skin 0.17 protein_coding synonymous_variant LOW 1080T>C Ile360Ile
M0076224 KHGDHFHA_00012 11379 3 Skin 0.17 protein_coding synonymous_variant LOW 132A>G Arg44Arg
M0076225 KHGDHFHA_00014 13304 5 Skin 0.28 protein_coding missense_variant MODERATE 363G>C Leu121Phe
M0076226 KHGDHFHA_00014 13426 4 Skin 0.22 protein_coding missense_variant MODERATE 485A>C Asp162Ala
M0076227 KHGDHFHA_00011 10892 3 Skin 0.17 protein_coding missense_variant MODERATE 232G>A Val78Ile
M0076228 KHGDHFHA_00011 11066 3 Skin 0.17 protein_coding missense_variant MODERATE 406C>T Pro136Ser






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group
KHGDHFHA_00015 Cobalt (Co), Nickel (Ni) 98.3 5e-62 1 119 1.0000 1.0000 prediction






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term