Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C3245
  Reference Plasmid   MET0639_bin.25__k81_90163
  Reference Plasmid Size   3559
  Reference Plasmid GC Content   0.76
  Reference Plasmid Mobility Type   non-mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0081590 KFEAHOKJ_00001 119 7 Skin 0.64 protein_coding upstream_gene_variant MODIFIER -95T>C None
M0081591 KFEAHOKJ_00001 131 6 Skin 0.55 protein_coding upstream_gene_variant MODIFIER -83C>G None
M0081592 KFEAHOKJ_00001 153 5 Skin 0.45 protein_coding upstream_gene_variant MODIFIER -61T>C None
M0081593 KFEAHOKJ_00001 171 5 Skin 0.45 protein_coding upstream_gene_variant MODIFIER -43T>G None
M0081594 KFEAHOKJ_00001 174 3 Skin 0.27 protein_coding upstream_gene_variant MODIFIER -40C>T None
M0081595 KFEAHOKJ_00001 182 4 Skin 0.36 protein_coding upstream_gene_variant MODIFIER -32G>C None
M0081596 KFEAHOKJ_00001 187 5 Skin 0.45 protein_coding upstream_gene_variant MODIFIER -27C>G None
M0081597 KFEAHOKJ_00001 211 4 Skin 0.36 protein_coding upstream_gene_variant MODIFIER -3G>T None
M0081598 KFEAHOKJ_00001 537 4 Skin 0.36 protein_coding missense_variant MODERATE 324C>G Asp108Glu
M0081599 KFEAHOKJ_00001 834 3 Skin 0.27 protein_coding synonymous_variant LOW 621C>G Gly207Gly
M0081600 KFEAHOKJ_00001 921 9 Skin 0.82 protein_coding synonymous_variant LOW 708A>G Gly236Gly
M0081601 KFEAHOKJ_00001 975 7 Skin 0.64 protein_coding synonymous_variant LOW 762T>C Gly254Gly
M0081602 KFEAHOKJ_00001 1012 6 Skin 0.55 protein_coding missense_variant MODERATE 799C>A Gln267Lys
M0081603 KFEAHOKJ_00001 1039 11 Skin 1.00 protein_coding missense_variant MODERATE 826G>A Ala276Thr
M0081604 KFEAHOKJ_00001 1065 8 Skin 0.73 protein_coding synonymous_variant LOW 852C>G Pro284Pro
M0081605 KFEAHOKJ_00001 1132 5 Skin 0.45 protein_coding missense_variant MODERATE 919A>G Ile307Val
M0081606 KFEAHOKJ_00001 1140 7 Skin 0.64 protein_coding synonymous_variant LOW 927G>T Arg309Arg
M0081607 KFEAHOKJ_00001 1156 8 Skin 0.73 protein_coding missense_variant MODERATE 943C>G Gln315Glu
M0081608 KFEAHOKJ_00001 1179 11 Skin 1.00 protein_coding synonymous_variant LOW 966T>C Gly322Gly
M0081609 KFEAHOKJ_00001 1475 3 Skin 0.27 protein_coding downstream_gene_variant MODIFIER *5A>G None
M0081610 KFEAHOKJ_00002 1913 6 Skin 0.55 protein_coding synonymous_variant LOW 456C>G Leu152Leu
M0081611 KFEAHOKJ_00001 672 3 Skin 0.27 protein_coding synonymous_variant LOW 459C>G Ala153Ala
M0081612 KFEAHOKJ_00001 726 3 Skin 0.27 protein_coding synonymous_variant LOW 513C>G Ser171Ser
M0081613 KFEAHOKJ_00001 738 3 Skin 0.27 protein_coding synonymous_variant LOW 525C>G Ser175Ser
M0081614 KFEAHOKJ_00001 924 4 Skin 0.36 protein_coding synonymous_variant LOW 711G>C Pro237Pro
M0081615 KFEAHOKJ_00001 1168 3 Skin 0.27 protein_coding missense_variant MODERATE 955G>C Asp319His
M0081616 KFEAHOKJ_00002 1679 3 Skin 0.27 protein_coding synonymous_variant LOW 690G>C Leu230Leu
M0081617 KFEAHOKJ_00002 1793 4 Skin 0.36 protein_coding synonymous_variant LOW 576T>G Gly192Gly
M0081618 KFEAHOKJ_00002 1832 3 Skin 0.27 protein_coding synonymous_variant LOW 537C>A Ala179Ala
M0081619 KFEAHOKJ_00002 2033 3 Skin 0.27 protein_coding synonymous_variant LOW 336A>G Ala112Ala
M0081620 KFEAHOKJ_00002 2129 5 Skin 0.45 protein_coding synonymous_variant LOW 240G>C Leu80Leu
M0081621 KFEAHOKJ_00002 2210 6 Skin 0.55 protein_coding synonymous_variant LOW 159T>C Ala53Ala
M0081622 KFEAHOKJ_00002 2225 6 Skin 0.55 protein_coding synonymous_variant LOW 144G>C Gly48Gly
M0081623 KFEAHOKJ_00002 2355 4 Skin 0.36 protein_coding missense_variant MODERATE 14T>C Leu5Pro
M0081624 KFEAHOKJ_00003 2453 5 Skin 0.45 protein_coding missense_variant MODERATE 932G>A Gly311Asp
M0081625 KFEAHOKJ_00003 2662 4 Skin 0.36 protein_coding synonymous_variant LOW 723G>C Thr241Thr
M0081626 KFEAHOKJ_00003 2886 5 Skin 0.45 protein_coding missense_variant MODERATE 499C>G Gln167Glu
M0081627 KFEAHOKJ_00003 2890 5 Skin 0.45 protein_coding synonymous_variant LOW 495A>G Ala165Ala
M0081628 KFEAHOKJ_00003 2923 5 Skin 0.45 protein_coding synonymous_variant LOW 462T>C Arg154Arg
M0081629 KFEAHOKJ_00003 3259 3 Skin 0.27 protein_coding synonymous_variant LOW 126T>C Gly42Gly
M0081630 KFEAHOKJ_00003 3278 3 Skin 0.27 protein_coding missense_variant MODERATE 107C>G Thr36Arg
M0081631 KFEAHOKJ_00003 3279 3 Skin 0.27 protein_coding missense_variant MODERATE 106A>G Thr36Ala
M0081632 KFEAHOKJ_00004 3425 3 Skin 0.27 protein_coding missense_variant MODERATE 133G>T Ala45Ser
M0081633 KFEAHOKJ_00001 318 4 Skin 0.36 protein_coding synonymous_variant LOW 105C>G Ala35Ala
M0081634 KFEAHOKJ_00001 357 4 Skin 0.36 protein_coding synonymous_variant LOW 144T>C Gly48Gly
M0081635 KFEAHOKJ_00001 365 3 Skin 0.27 protein_coding missense_variant MODERATE 152A>T Glu51Val
M0081636 KFEAHOKJ_00001 507 5 Skin 0.45 protein_coding synonymous_variant LOW 294T>C Arg98Arg
M0081637 KFEAHOKJ_00001 972 3 Skin 0.27 protein_coding synonymous_variant LOW 759C>A Ile253Ile
M0081638 KFEAHOKJ_00001 1224 4 Skin 0.36 protein_coding synonymous_variant LOW 1011G>C Ala337Ala
M0081639 KFEAHOKJ_00003 3040 3 Skin 0.27 protein_coding synonymous_variant LOW 345C>G Ala115Ala
M0081640 KFEAHOKJ_00003 3043 3 Skin 0.27 protein_coding synonymous_variant LOW 342G>A Gly114Gly






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term