Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C3253
  Reference Plasmid   MET0658_bin.47__k81_11735
  Reference Plasmid Size   4348
  Reference Plasmid GC Content   0.74
  Reference Plasmid Mobility Type   non-mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0082064 GNCOJGLF_00001 102 8 Skin 0.73 protein_coding missense_variant MODERATE 74G>A Gly25Asp
M0082065 GNCOJGLF_00001 157 5 Skin 0.45 protein_coding missense_variant MODERATE 129C>G His43Gln
M0082066 GNCOJGLF_00001 502 9 Skin 0.82 protein_coding synonymous_variant LOW 474A>G Thr158Thr
M0082067 GNCOJGLF_00001 646 8 Skin 0.73 protein_coding synonymous_variant LOW 618T>C Thr206Thr
M0082068 GNCOJGLF_00001 758 7 Skin 0.64 protein_coding missense_variant MODERATE 730G>A Val244Ile
M0082069 GNCOJGLF_00001 759 6 Skin 0.55 protein_coding missense_variant MODERATE 731T>C Val244Ala
M0082070 GNCOJGLF_00001 766 4 Skin 0.36 protein_coding synonymous_variant LOW 738C>T Tyr246Tyr
M0082071 GNCOJGLF_00001 778 7 Skin 0.64 protein_coding synonymous_variant LOW 750A>G Ala250Ala
M0082072 GNCOJGLF_00002 858 7 Skin 0.64 protein_coding synonymous_variant LOW 18G>A Glu6Glu
M0082073 GNCOJGLF_00003 1328 6 Skin 0.55 protein_coding synonymous_variant LOW 24T>C Asp8Asp
M0082074 GNCOJGLF_00003 1528 3 Skin 0.27 protein_coding missense_variant MODERATE 224G>C Gly75Ala
M0082075 GNCOJGLF_00003 1533 9 Skin 0.82 protein_coding missense_variant MODERATE 229A>G Ile77Val
M0082076 GNCOJGLF_00003 1559 3 Skin 0.27 protein_coding synonymous_variant LOW 255C>T Tyr85Tyr
M0082077 GNCOJGLF_00003 1565 10 Skin 0.91 protein_coding synonymous_variant LOW 261T>G Ala87Ala
M0082078 GNCOJGLF_00003 1595 9 Skin 0.82 protein_coding synonymous_variant LOW 291T>C Asp97Asp
M0082079 GNCOJGLF_00003 1607 6 Skin 0.55 protein_coding synonymous_variant LOW 303C>G Arg101Arg
M0082080 GNCOJGLF_00003 1636 9 Skin 0.82 protein_coding missense_variant MODERATE 332T>A Leu111His
M0082081 GNCOJGLF_00003 1646 8 Skin 0.73 protein_coding missense_variant MODERATE 342G>C Gln114His
M0082082 GNCOJGLF_00003 1689 3 Skin 0.27 protein_coding synonymous_variant LOW 385C>T Leu129Leu
M0082083 GNCOJGLF_00003 1756 3 Skin 0.27 protein_coding missense_variant MODERATE 452T>C Val151Ala
M0082084 GNCOJGLF_00003 1904 3 Skin 0.27 protein_coding synonymous_variant LOW 600T>G Ala200Ala
M0082085 GNCOJGLF_00003 1942 8 Skin 0.73 protein_coding missense_variant MODERATE 638G>A Gly213Asp
M0082086 GNCOJGLF_00004 2064 5 Skin 0.45 protein_coding upstream_gene_variant MODIFIER -94G>T None
M0082087 GNCOJGLF_00004 2190 5 Skin 0.45 protein_coding synonymous_variant LOW 33T>C Arg11Arg
M0082088 GNCOJGLF_00004 2214 4 Skin 0.36 protein_coding synonymous_variant LOW 57C>G Leu19Leu
M0082089 GNCOJGLF_00004 2445 5 Skin 0.45 protein_coding synonymous_variant LOW 288T>C Asp96Asp
M0082090 GNCOJGLF_00004 2735 4 Skin 0.36 protein_coding missense_variant MODERATE 578C>T Ala193Val
M0082091 GNCOJGLF_00004 2741 4 Skin 0.36 protein_coding missense_variant MODERATE 584T>C Val195Ala
M0082092 GNCOJGLF_00004 2838 6 Skin 0.55 protein_coding synonymous_variant LOW 681A>G Val227Val
M0082093 GNCOJGLF_00004 2962 4 Skin 0.36 protein_coding missense_variant MODERATE 805T>G Ser269Ala
M0082094 GNCOJGLF_00004 2970 4 Skin 0.36 protein_coding synonymous_variant LOW 813A>G Glu271Glu
M0082095 GNCOJGLF_00004 2991 3 Skin 0.27 protein_coding synonymous_variant LOW 834G>T Ala278Ala
M0082096 GNCOJGLF_00004 3180 5 Skin 0.45 protein_coding synonymous_variant LOW 1023C>G Pro341Pro
M0082097 GNCOJGLF_00005 3219 4 Skin 0.36 protein_coding missense_variant MODERATE 34A>G Ser12Gly
M0082098 GNCOJGLF_00005 3231 4 Skin 0.36 protein_coding missense_variant MODERATE 46A>G Thr16Ala
M0082099 GNCOJGLF_00005 3362 4 Skin 0.36 protein_coding synonymous_variant LOW 177T>C Gly59Gly
M0082100 GNCOJGLF_00005 3506 4 Skin 0.36 protein_coding synonymous_variant LOW 321T>C Leu107Leu
M0082101 GNCOJGLF_00005 3509 3 Skin 0.27 protein_coding synonymous_variant LOW 324A>G Ala108Ala
M0082102 GNCOJGLF_00005 3569 3 Skin 0.27 protein_coding synonymous_variant LOW 384C>G Gly128Gly
M0082103 GNCOJGLF_00005 3650 4 Skin 0.36 protein_coding synonymous_variant LOW 465A>G Glu155Glu
M0082104 GNCOJGLF_00001 305 5 Skin 0.45 protein_coding missense_variant MODERATE 277T>C Phe93Leu
M0082105 GNCOJGLF_00001 427 5 Skin 0.45 protein_coding synonymous_variant LOW 399G>C Thr133Thr
M0082106 GNCOJGLF_00001 493 5 Skin 0.45 protein_coding synonymous_variant LOW 465C>T Arg155Arg
M0082107 GNCOJGLF_00001 577 4 Skin 0.36 protein_coding synonymous_variant LOW 549C>G Pro183Pro
M0082108 GNCOJGLF_00001 584 6 Skin 0.55 protein_coding missense_variant MODERATE 556A>G Thr186Ala
M0082109 GNCOJGLF_00002 1053 3 Skin 0.27 protein_coding synonymous_variant LOW 213G>A Ala71Ala
M0082110 GNCOJGLF_00002 1056 7 Skin 0.64 protein_coding synonymous_variant LOW 216G>C Gly72Gly
M0082111 GNCOJGLF_00002 1188 8 Skin 0.73 protein_coding synonymous_variant LOW 348G>C Val116Val
M0082112 GNCOJGLF_00003 1787 5 Skin 0.45 protein_coding missense_variant MODERATE 483C>G Asp161Glu
M0082113 GNCOJGLF_00003 1865 4 Skin 0.36 protein_coding synonymous_variant LOW 561G>A Glu187Glu
M0082114 GNCOJGLF_00002 1110 5 Skin 0.45 protein_coding synonymous_variant LOW 270C>G Leu90Leu
M0082115 GNCOJGLF_00002 1159 6 Skin 0.55 protein_coding synonymous_variant LOW 319C>T Leu107Leu
M0082116 GNCOJGLF_00003 1530 3 Skin 0.27 protein_coding missense_variant MODERATE 226G>A Val76Met
M0082117 GNCOJGLF_00003 1561 5 Skin 0.45 protein_coding missense_variant MODERATE 257A>G Asp86Gly
M0082118 GNCOJGLF_00003 1701 4 Skin 0.36 protein_coding missense_variant MODERATE 397C>G Leu133Val
M0082119 GNCOJGLF_00003 1825 4 Skin 0.36 protein_coding missense_variant MODERATE 521C>T Ala174Val
M0082120 GNCOJGLF_00003 1893 3 Skin 0.27 protein_coding missense_variant MODERATE 589G>A Ala197Thr
M0082121 GNCOJGLF_00003 1895 3 Skin 0.27 protein_coding synonymous_variant LOW 591C>T Ala197Ala
M0082122 GNCOJGLF_00003 1919 4 Skin 0.36 protein_coding missense_variant MODERATE 615G>C Glu205Asp
M0082123 GNCOJGLF_00004 2523 5 Skin 0.45 protein_coding synonymous_variant LOW 366C>G Leu122Leu
M0082124 GNCOJGLF_00004 2526 3 Skin 0.27 protein_coding missense_variant MODERATE 369C>G Asp123Glu
M0082125 GNCOJGLF_00004 2545 3 Skin 0.27 protein_coding missense_variant MODERATE 388G>A Ala130Thr
M0082126 GNCOJGLF_00002 924 3 Skin 0.27 protein_coding synonymous_variant LOW 84C>T Arg28Arg
M0082127 GNCOJGLF_00003 1892 3 Skin 0.27 protein_coding synonymous_variant LOW 588C>T Ala196Ala
M0082128 GNCOJGLF_00004 2377 3 Skin 0.27 protein_coding missense_variant MODERATE 220G>A Val74Ile
M0082129 GNCOJGLF_00004 2811 3 Skin 0.27 protein_coding synonymous_variant LOW 654G>C Leu218Leu






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term