Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C3281
  Reference Plasmid   MET0772_bin.8__k81_263687
  Reference Plasmid Size   6156
  Reference Plasmid GC Content   0.52
  Reference Plasmid Mobility Type   non-mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0133782 EDLMBGIJ_00002 5005 4 Oral 1.00 protein_coding synonymous_variant LOW 27G>A Val9Val
M0133783 EDLMBGIJ_00002 5044 3 Oral 0.75 protein_coding synonymous_variant LOW 66A>G Leu22Leu
M0133784 EDLMBGIJ_00002 5107 4 Oral 1.00 protein_coding synonymous_variant LOW 129T>C Tyr43Tyr
M0133785 EDLMBGIJ_00002 5153 4 Oral 1.00 protein_coding missense_variant MODERATE 175A>G Ile59Val
M0133786 EDLMBGIJ_00002 5161 4 Oral 1.00 protein_coding synonymous_variant LOW 183C>T Cys61Cys
M0133787 EDLMBGIJ_00002 5167 4 Oral 1.00 protein_coding synonymous_variant LOW 189C>T Pro63Pro
M0133788 EDLMBGIJ_00002 5204 4 Oral 1.00 protein_coding missense_variant MODERATE 226C>G Pro76Ala
M0133789 EDLMBGIJ_00002 5212 4 Oral 1.00 protein_coding synonymous_variant LOW 234T>C Val78Val
M0133790 EDLMBGIJ_00002 5328 4 Oral 1.00 protein_coding missense_variant MODERATE 350T>A Leu117His
M0133791 EDLMBGIJ_00002 5348 4 Oral 1.00 protein_coding missense_variant MODERATE 370A>G Lys124Glu
M0133792 EDLMBGIJ_00001 136 3 Oral 0.75 protein_coding synonymous_variant LOW 111T>A Ile37Ile
M0133793 EDLMBGIJ_00001 151 3 Oral 0.75 protein_coding synonymous_variant LOW 126C>T Cys42Cys
M0133794 EDLMBGIJ_00001 154 3 Oral 0.75 protein_coding synonymous_variant LOW 129T>C Arg43Arg
M0133795 EDLMBGIJ_00001 195 3 Oral 0.75 protein_coding missense_variant MODERATE 170A>G Asp57Gly
M0133796 EDLMBGIJ_00001 196 3 Oral 0.75 protein_coding synonymous_variant LOW 171T>C Asp57Asp
M0133797 EDLMBGIJ_00001 209 3 Oral 0.75 protein_coding synonymous_variant LOW 184T>C Leu62Leu
M0133798 EDLMBGIJ_00001 211 3 Oral 0.75 protein_coding synonymous_variant LOW 186G>A Leu62Leu
M0133799 EDLMBGIJ_00001 241 3 Oral 0.75 protein_coding synonymous_variant LOW 216T>C Ala72Ala
M0133800 EDLMBGIJ_00001 382 3 Oral 0.75 protein_coding synonymous_variant LOW 357T>C Val119Val
M0133801 EDLMBGIJ_00001 484 3 Oral 0.75 protein_coding synonymous_variant LOW 459C>T Val153Val
M0133802 EDLMBGIJ_00001 491 3 Oral 0.75 protein_coding missense_variant MODERATE 466G>A Val156Ile
M0133803 EDLMBGIJ_00001 550 3 Oral 0.75 protein_coding synonymous_variant LOW 525T>C Ile175Ile
M0133804 EDLMBGIJ_00001 641 3 Oral 0.75 protein_coding missense_variant MODERATE 616A>C Ser206Arg
M0133805 EDLMBGIJ_00001 682 3 Oral 0.75 protein_coding synonymous_variant LOW 657T>G Leu219Leu
M0133806 EDLMBGIJ_00001 685 3 Oral 0.75 protein_coding synonymous_variant LOW 660T>G Ala220Ala
M0133807 EDLMBGIJ_00001 691 3 Oral 0.75 protein_coding synonymous_variant LOW 666A>T Gly222Gly
M0133808 EDLMBGIJ_00001 760 3 Oral 0.75 protein_coding synonymous_variant LOW 735A>G Val245Val
M0133809 EDLMBGIJ_00001 865 3 Oral 0.75 protein_coding synonymous_variant LOW 840C>T Arg280Arg
M0133810 EDLMBGIJ_00001 889 3 Oral 0.75 protein_coding synonymous_variant LOW 864T>C Pro288Pro
M0133811 EDLMBGIJ_00001 992 3 Oral 0.75 protein_coding missense_variant MODERATE 967A>G Thr323Ala
M0133812 EDLMBGIJ_00001 994 3 Oral 0.75 protein_coding synonymous_variant LOW 969G>A Thr323Thr
M0133813 EDLMBGIJ_00001 1141 3 Oral 0.75 protein_coding synonymous_variant LOW 1116A>G Lys372Lys
M0133814 EDLMBGIJ_00001 1228 3 Oral 0.75 protein_coding synonymous_variant LOW 1203T>C Ser401Ser
M0133815 EDLMBGIJ_00001 1396 3 Oral 0.75 protein_coding synonymous_variant LOW 1371C>T Leu457Leu
M0133816 EDLMBGIJ_00001 1420 3 Oral 0.75 protein_coding synonymous_variant LOW 1395G>T Thr465Thr
M0133817 EDLMBGIJ_00001 1460 3 Oral 0.75 protein_coding missense_variant MODERATE 1435A>G Thr479Ala
M0133818 EDLMBGIJ_00001 1645 3 Oral 0.75 protein_coding synonymous_variant LOW 1620T>C Asn540Asn
M0133819 EDLMBGIJ_00001 1763 3 Oral 0.75 protein_coding synonymous_variant LOW 1738T>C Leu580Leu
M0133820 EDLMBGIJ_00001 1777 3 Oral 0.75 protein_coding synonymous_variant LOW 1752A>T Leu584Leu
M0133821 EDLMBGIJ_00001 1973 3 Oral 0.75 protein_coding missense_variant MODERATE 1948A>G Thr650Ala
M0133822 EDLMBGIJ_00001 1984 3 Oral 0.75 protein_coding synonymous_variant LOW 1959T>A Ala653Ala
M0133823 EDLMBGIJ_00001 2001 3 Oral 0.75 protein_coding missense_variant MODERATE 1976G>T Arg659Ile
M0133824 EDLMBGIJ_00001 2009 3 Oral 0.75 protein_coding synonymous_variant LOW 1984C>T Leu662Leu
M0133825 EDLMBGIJ_00001 2186 3 Oral 0.75 protein_coding missense_variant MODERATE 2161G>A Val721Ile
M0133826 EDLMBGIJ_00001 2414 3 Oral 0.75 protein_coding missense_variant MODERATE 2389A>G Thr797Ala
M0133827 EDLMBGIJ_00001 2514 3 Oral 0.75 protein_coding missense_variant MODERATE 2489T>A Val830Glu
M0133828 EDLMBGIJ_00001 2537 3 Oral 0.75 protein_coding missense_variant MODERATE 2512A>C Lys838Gln
M0133829 EDLMBGIJ_00001 2779 3 Oral 0.75 protein_coding synonymous_variant LOW 2754C>T Ser918Ser
M0133830 EDLMBGIJ_00001 2785 3 Oral 0.75 protein_coding synonymous_variant LOW 2760T>C Leu920Leu
M0133831 EDLMBGIJ_00001 2888 3 Oral 0.75 protein_coding synonymous_variant LOW 2863T>C Leu955Leu
M0133832 EDLMBGIJ_00001 3115 3 Oral 0.75 protein_coding synonymous_variant LOW 3090T>C Asp1030Asp
M0133833 EDLMBGIJ_00001 3334 3 Oral 0.75 protein_coding synonymous_variant LOW 3309T>C Ser1103Ser
M0133834 EDLMBGIJ_00001 3385 3 Oral 0.75 protein_coding synonymous_variant LOW 3360G>A Glu1120Glu
M0133835 EDLMBGIJ_00001 3403 3 Oral 0.75 protein_coding synonymous_variant LOW 3378A>T Ser1126Ser
M0133836 EDLMBGIJ_00001 3406 3 Oral 0.75 protein_coding synonymous_variant LOW 3381A>G Val1127Val
M0133837 EDLMBGIJ_00001 3643 3 Oral 0.75 protein_coding missense_variant MODERATE 3618T>G Phe1206Leu
M0133838 EDLMBGIJ_00001 3646 3 Oral 0.75 protein_coding synonymous_variant LOW 3621C>A Ala1207Ala
M0133839 EDLMBGIJ_00001 3871 3 Oral 0.75 protein_coding synonymous_variant LOW 3846T>C Ile1282Ile
M0133840 EDLMBGIJ_00001 3922 3 Oral 0.75 protein_coding synonymous_variant LOW 3897C>T Leu1299Leu
M0133841 EDLMBGIJ_00001 3964 3 Oral 0.75 protein_coding synonymous_variant LOW 3939T>C Pro1313Pro
M0133842 EDLMBGIJ_00001 4045 3 Oral 0.75 protein_coding synonymous_variant LOW 4020A>G Gln1340Gln
M0133843 EDLMBGIJ_00001 4436 3 Oral 0.75 protein_coding missense_variant MODERATE 4411T>G Tyr1471Asp
M0133844 EDLMBGIJ_00001 4482 3 Oral 0.75 protein_coding missense_variant MODERATE 4457T>C Leu1486Ser
M0133845 EDLMBGIJ_00001 4491 3 Oral 0.75 protein_coding missense_variant MODERATE 4466T>C Leu1489Ser
M0133846 EDLMBGIJ_00001 4516 3 Oral 0.75 protein_coding synonymous_variant LOW 4491T>C Ser1497Ser
M0133847 EDLMBGIJ_00002 5314 3 Oral 0.75 protein_coding synonymous_variant LOW 336A>G Glu112Glu
M0133848 EDLMBGIJ_00002 5428 3 Oral 0.75 protein_coding synonymous_variant LOW 450C>T Ile150Ile
M0133849 EDLMBGIJ_00002 5455 3 Oral 0.75 protein_coding synonymous_variant LOW 477C>T Arg159Arg
M0133850 EDLMBGIJ_00002 5472 3 Oral 0.75 protein_coding missense_variant MODERATE 494A>C Asn165Thr
M0133851 EDLMBGIJ_00002 5473 3 Oral 0.75 protein_coding missense_variant MODERATE 495T>A Asn165Lys
M0133852 EDLMBGIJ_00002 5477 3 Oral 0.75 protein_coding missense_variant MODERATE 499A>G Thr167Ala
M0133853 EDLMBGIJ_00002 5479 3 Oral 0.75 protein_coding synonymous_variant LOW 501C>T Thr167Thr
M0133854 EDLMBGIJ_00002 5481 3 Oral 0.75 protein_coding missense_variant MODERATE 503T>C Leu168Ser






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term