Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C3282
  Reference Plasmid   MET0773_bin.1__k81_2933
  Reference Plasmid Size   8907
  Reference Plasmid GC Content   0.64
  Reference Plasmid Mobility Type   non-mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0083596 NHJEIEBE_00001 652 3 Skin 0.14 protein_coding synonymous_variant LOW 276C>T Arg92Arg
M0083597 NHJEIEBE_00001 922 3 Skin 0.14 protein_coding synonymous_variant LOW 546A>G Gly182Gly
M0083598 NHJEIEBE_00005 1377 3 Skin 0.14 protein_coding upstream_gene_variant MODIFIER -3588A>G None
M0083599 NHJEIEBE_00005 1424 3 Skin 0.14 protein_coding upstream_gene_variant MODIFIER -3541A>G None
M0083600 NHJEIEBE_00005 1550 3 Skin 0.14 protein_coding upstream_gene_variant MODIFIER -3415A>G None
M0083601 NHJEIEBE_00005 1702 4 Skin 0.19 protein_coding upstream_gene_variant MODIFIER -3263T>C None
M0083602 NHJEIEBE_00005 1706 3 Skin 0.14 protein_coding upstream_gene_variant MODIFIER -3259A>G None
M0083603 NHJEIEBE_00002 1785 3 Skin 0.14 protein_coding missense_variant MODERATE 526G>A Ala176Thr
M0083604 NHJEIEBE_00002 1786 3 Skin 0.14 protein_coding synonymous_variant LOW 525T>C Ala175Ala
M0083605 NHJEIEBE_00002 1867 5 Skin 0.24 protein_coding synonymous_variant LOW 444T>C Leu148Leu
M0083606 NHJEIEBE_00002 2002 3 Skin 0.14 protein_coding synonymous_variant LOW 309G>A Pro103Pro
M0083607 NHJEIEBE_00002 2014 3 Skin 0.14 protein_coding synonymous_variant LOW 297T>C Ala99Ala
M0083608 NHJEIEBE_00002 2092 3 Skin 0.14 protein_coding synonymous_variant LOW 219G>A Gly73Gly
M0083609 NHJEIEBE_00002 2099 3 Skin 0.14 protein_coding missense_variant MODERATE 212G>A Arg71Lys
M0083610 NHJEIEBE_00002 2100 3 Skin 0.14 protein_coding missense_variant MODERATE 211A>G Arg71Gly
M0083611 NHJEIEBE_00002 2122 4 Skin 0.19 protein_coding synonymous_variant LOW 189T>C His63His
M0083612 NHJEIEBE_00003 2348 3 Skin 0.14 protein_coding synonymous_variant LOW 1125T>G Arg375Arg
M0083613 NHJEIEBE_00003 2370 7 Skin 0.33 protein_coding missense_variant MODERATE 1103G>A Gly368Asp
M0083614 NHJEIEBE_00003 2435 12 Skin 0.57 protein_coding synonymous_variant LOW 1038A>G Val346Val
M0083615 NHJEIEBE_00003 2498 3 Skin 0.14 protein_coding synonymous_variant LOW 975C>T Gly325Gly
M0083616 NHJEIEBE_00003 2504 8 Skin 0.38 protein_coding synonymous_variant LOW 969T>C Leu323Leu
M0083617 NHJEIEBE_00003 2515 6 Skin 0.29 protein_coding missense_variant MODERATE 958A>G Thr320Ala
M0083618 NHJEIEBE_00003 2528 3 Skin 0.14 protein_coding synonymous_variant LOW 945T>C Pro315Pro
M0083619 NHJEIEBE_00003 2531 11 Skin 0.52 protein_coding synonymous_variant LOW 942T>C Ala314Ala
M0083620 NHJEIEBE_00003 2534 11 Skin 0.52 protein_coding synonymous_variant LOW 939T>C Thr313Thr
M0083621 NHJEIEBE_00003 2627 6 Skin 0.29 protein_coding synonymous_variant LOW 846C>T Thr282Thr
M0083622 NHJEIEBE_00003 2720 8 Skin 0.38 protein_coding synonymous_variant LOW 753A>G Arg251Arg
M0083623 NHJEIEBE_00003 2764 12 Skin 0.57 protein_coding missense_variant MODERATE 709T>C Trp237Arg
M0083624 NHJEIEBE_00003 2909 3 Skin 0.14 protein_coding synonymous_variant LOW 564G>A Glu188Glu
M0083625 NHJEIEBE_00003 2933 7 Skin 0.33 protein_coding synonymous_variant LOW 540A>G Gln180Gln
M0083626 NHJEIEBE_00003 3095 10 Skin 0.48 protein_coding synonymous_variant LOW 378T>C Asp126Asp
M0083627 NHJEIEBE_00003 3191 5 Skin 0.24 protein_coding synonymous_variant LOW 282T>C Pro94Pro
M0083628 NHJEIEBE_00003 3194 5 Skin 0.24 protein_coding synonymous_variant LOW 279C>T Arg93Arg
M0083629 NHJEIEBE_00003 3197 5 Skin 0.24 protein_coding synonymous_variant LOW 276A>G Val92Val
M0083630 NHJEIEBE_00003 3200 4 Skin 0.19 protein_coding synonymous_variant LOW 273A>G Ala91Ala
M0083631 NHJEIEBE_00003 3213 5 Skin 0.24 protein_coding missense_variant MODERATE 260A>C Glu87Ala
M0083632 NHJEIEBE_00003 3276 13 Skin 0.62 protein_coding missense_variant MODERATE 197C>A Ala66Asp
M0083633 NHJEIEBE_00003 3353 8 Skin 0.38 protein_coding synonymous_variant LOW 120G>A Glu40Glu
M0083634 NHJEIEBE_00003 3373 11 Skin 0.52 protein_coding synonymous_variant LOW 100T>C Leu34Leu
M0083635 NHJEIEBE_00003 3383 8 Skin 0.38 protein_coding synonymous_variant LOW 90T>C Arg30Arg
M0083636 NHJEIEBE_00003 3394 11 Skin 0.52 protein_coding synonymous_variant LOW 79T>C Leu27Leu
M0083637 NHJEIEBE_00002 3756 3 Skin 0.14 protein_coding upstream_gene_variant MODIFIER -1446C>T None
M0083638 NHJEIEBE_00002 3862 4 Skin 0.19 protein_coding upstream_gene_variant MODIFIER -1552A>G None
M0083639 NHJEIEBE_00002 3955 9 Skin 0.43 protein_coding upstream_gene_variant MODIFIER -1645G>A None
M0083640 NHJEIEBE_00002 3965 7 Skin 0.33 protein_coding upstream_gene_variant MODIFIER -1655C>T None
M0083641 NHJEIEBE_00004 4030 9 Skin 0.43 protein_coding synonymous_variant LOW 759A>T Pro253Pro
M0083642 NHJEIEBE_00004 4234 18 Skin 0.86 protein_coding synonymous_variant LOW 555T>G Leu185Leu
M0083643 NHJEIEBE_00004 4255 6 Skin 0.29 protein_coding synonymous_variant LOW 534T>C Ala178Ala
M0083644 NHJEIEBE_00004 4324 13 Skin 0.62 protein_coding synonymous_variant LOW 465T>C Gly155Gly
M0083645 NHJEIEBE_00004 4417 10 Skin 0.48 protein_coding synonymous_variant LOW 372A>G Gly124Gly
M0083646 NHJEIEBE_00004 4432 9 Skin 0.43 protein_coding synonymous_variant LOW 357G>C Val119Val
M0083647 NHJEIEBE_00004 4485 8 Skin 0.38 protein_coding synonymous_variant LOW 304C>T Leu102Leu
M0083648 NHJEIEBE_00004 4501 18 Skin 0.86 protein_coding synonymous_variant LOW 288T>C Arg96Arg
M0083649 NHJEIEBE_00004 4564 11 Skin 0.52 protein_coding synonymous_variant LOW 225A>T Ala75Ala
M0083650 NHJEIEBE_00003 3032 5 Skin 0.24 protein_coding synonymous_variant LOW 441C>T Ser147Ser
M0083651 NHJEIEBE_00003 3056 8 Skin 0.38 protein_coding synonymous_variant LOW 417A>G Gly139Gly
M0083652 NHJEIEBE_00003 3066 7 Skin 0.33 protein_coding missense_variant MODERATE 407A>G Glu136Gly
M0083653 NHJEIEBE_00003 3134 8 Skin 0.38 protein_coding synonymous_variant LOW 339G>A Glu113Glu
M0083654 NHJEIEBE_00003 3437 9 Skin 0.43 protein_coding synonymous_variant LOW 36G>A Gly12Gly
M0083655 NHJEIEBE_00002 3961 6 Skin 0.29 protein_coding upstream_gene_variant MODIFIER -1651T>C None
M0083656 NHJEIEBE_00004 4003 5 Skin 0.24 protein_coding synonymous_variant LOW 786G>A Glu262Glu
M0083657 NHJEIEBE_00004 4054 5 Skin 0.24 protein_coding synonymous_variant LOW 735G>A Gly245Gly
M0083658 NHJEIEBE_00004 4112 13 Skin 0.62 protein_coding missense_variant MODERATE 677A>G Gln226Arg
M0083659 NHJEIEBE_00004 4246 3 Skin 0.14 protein_coding synonymous_variant LOW 543C>T Gly181Gly
M0083660 NHJEIEBE_00004 4725 10 Skin 0.48 protein_coding missense_variant MODERATE 64T>C Ser22Pro
M0083661 NHJEIEBE_00004 4735 10 Skin 0.48 protein_coding synonymous_variant LOW 54G>A Arg18Arg
M0083662 NHJEIEBE_00002 3905 4 Skin 0.19 protein_coding upstream_gene_variant MODIFIER -1595A>G None
M0083663 NHJEIEBE_00002 3906 4 Skin 0.19 protein_coding upstream_gene_variant MODIFIER -1596G>T None
M0083664 NHJEIEBE_00002 3909 4 Skin 0.19 protein_coding upstream_gene_variant MODIFIER -1599A>C None
M0083665 NHJEIEBE_00004 4291 8 Skin 0.38 protein_coding synonymous_variant LOW 498A>G Gln166Gln
M0083666 NHJEIEBE_00007 6143 19 Skin 0.90 protein_coding missense_variant MODERATE 749C>T Ala250Val
M0083667 NHJEIEBE_00008 7619 6 Skin 0.29 protein_coding synonymous_variant LOW 189A>G Val63Val
M0083668 NHJEIEBE_00003 8218 3 Skin 0.14 protein_coding upstream_gene_variant MODIFIER -4746C>T None
M0083669 NHJEIEBE_00002 3679 3 Skin 0.14 protein_coding upstream_gene_variant MODIFIER -1369G>A None
M0083670 NHJEIEBE_00002 3912 7 Skin 0.33 protein_coding upstream_gene_variant MODIFIER -1602T>A None
M0083671 NHJEIEBE_00004 4587 6 Skin 0.29 protein_coding synonymous_variant LOW 202C>T Leu68Leu
M0083672 NHJEIEBE_00004 4588 6 Skin 0.29 protein_coding synonymous_variant LOW 201G>A Pro67Pro
M0083673 NHJEIEBE_00004 4603 6 Skin 0.29 protein_coding synonymous_variant LOW 186C>G Ser62Ser
M0083674 NHJEIEBE_00004 4642 6 Skin 0.29 protein_coding synonymous_variant LOW 147G>A Gly49Gly
M0083675 NHJEIEBE_00003 2690 5 Skin 0.24 protein_coding synonymous_variant LOW 783A>G Glu261Glu
M0083676 NHJEIEBE_00003 3248 3 Skin 0.14 protein_coding synonymous_variant LOW 225G>A Glu75Glu
M0083677 NHJEIEBE_00002 3847 3 Skin 0.14 protein_coding upstream_gene_variant MODIFIER -1537A>G None
M0083678 NHJEIEBE_00004 4075 7 Skin 0.33 protein_coding synonymous_variant LOW 714G>A Glu238Glu
M0083679 NHJEIEBE_00004 4171 10 Skin 0.48 protein_coding synonymous_variant LOW 618A>G Gln206Gln
M0083680 NHJEIEBE_00004 4595 4 Skin 0.19 protein_coding missense_variant MODERATE 194C>T Ser65Phe
M0083681 NHJEIEBE_00002 4829 4 Skin 0.19 protein_coding upstream_gene_variant MODIFIER -2519A>G None
M0083682 NHJEIEBE_00002 4831 4 Skin 0.19 protein_coding upstream_gene_variant MODIFIER -2521C>T None
M0083683 NHJEIEBE_00002 4849 4 Skin 0.19 protein_coding upstream_gene_variant MODIFIER -2539G>A None
M0083684 NHJEIEBE_00002 4852 4 Skin 0.19 protein_coding upstream_gene_variant MODIFIER -2542G>A None
M0083685 NHJEIEBE_00002 4855 4 Skin 0.19 protein_coding upstream_gene_variant MODIFIER -2545A>T None
M0083686 NHJEIEBE_00005 4998 4 Skin 0.19 protein_coding missense_variant MODERATE 34G>A Ala12Thr
M0083687 NHJEIEBE_00005 5132 4 Skin 0.19 protein_coding synonymous_variant LOW 168A>G Arg56Arg
M0083688 NHJEIEBE_00005 5138 4 Skin 0.19 protein_coding synonymous_variant LOW 174C>T His58His
M0083689 NHJEIEBE_00005 5213 4 Skin 0.19 protein_coding synonymous_variant LOW 249G>A Ser83Ser
M0083690 NHJEIEBE_00006 5330 4 Skin 0.19 protein_coding missense_variant MODERATE 83T>C Ile28Thr
M0083691 NHJEIEBE_00007 5556 4 Skin 0.19 protein_coding synonymous_variant LOW 162T>C Tyr54Tyr
M0083692 NHJEIEBE_00007 5694 4 Skin 0.19 protein_coding synonymous_variant LOW 300T>C Ser100Ser
M0083693 NHJEIEBE_00007 5846 4 Skin 0.19 protein_coding missense_variant MODERATE 452T>C Val151Ala
M0083694 NHJEIEBE_00007 5988 4 Skin 0.19 protein_coding synonymous_variant LOW 594G>T Leu198Leu
M0083695 NHJEIEBE_00007 6380 4 Skin 0.19 protein_coding missense_variant MODERATE 986C>T Ser329Phe
M0083696 NHJEIEBE_00007 6625 4 Skin 0.19 protein_coding missense_variant MODERATE 1231G>T Ala411Ser
M0083697 NHJEIEBE_00007 6730 4 Skin 0.19 protein_coding missense_variant MODERATE 1336A>G Thr446Ala
M0083698 NHJEIEBE_00007 6859 4 Skin 0.19 protein_coding missense_variant MODERATE 1465T>G Ser489Ala
M0083699 NHJEIEBE_00007 6916 4 Skin 0.19 protein_coding missense_variant MODERATE 1522A>G Thr508Ala
M0083700 NHJEIEBE_00007 6930 4 Skin 0.19 protein_coding synonymous_variant LOW 1536T>C Asp512Asp
M0083701 NHJEIEBE_00007 7037 4 Skin 0.19 protein_coding missense_variant MODERATE 1643C>T Ala548Val
M0083702 NHJEIEBE_00007 7071 5 Skin 0.24 protein_coding synonymous_variant LOW 1677T>C Ile559Ile
M0083703 NHJEIEBE_00007 7135 4 Skin 0.19 protein_coding missense_variant MODERATE 1741A>G Thr581Ala
M0083704 NHJEIEBE_00007 7158 3 Skin 0.14 protein_coding synonymous_variant LOW 1764G>C Thr588Thr
M0083705 NHJEIEBE_00007 7251 3 Skin 0.14 protein_coding synonymous_variant LOW 1857A>G Gln619Gln
M0083706 NHJEIEBE_00007 7296 3 Skin 0.14 protein_coding missense_variant MODERATE 1902G>A Met634Ile
M0083707 NHJEIEBE_00008 7444 3 Skin 0.14 protein_coding missense_variant MODERATE 14A>G Asn5Ser
M0083708 NHJEIEBE_00009 8355 3 Skin 0.14 protein_coding synonymous_variant LOW 291T>C Ile97Ile
M0083709 NHJEIEBE_00004 8703 3 Skin 0.14 protein_coding upstream_gene_variant MODIFIER -3915C>T None
M0083710 NHJEIEBE_00004 8727 3 Skin 0.14 protein_coding upstream_gene_variant MODIFIER -3939T>C None
M0083711 NHJEIEBE_00004 8732 4 Skin 0.19 protein_coding upstream_gene_variant MODIFIER -3944C>T None
M0083712 NHJEIEBE_00004 8769 3 Skin 0.14 protein_coding upstream_gene_variant MODIFIER -3981C>T None
M0083713 NHJEIEBE_00003 3466 4 Skin 0.19 protein_coding missense_variant MODERATE 7C>T Arg3Cys
M0083714 NHJEIEBE_00004 4222 10 Skin 0.48 protein_coding synonymous_variant LOW 567A>G Ser189Ser
M0083715 NHJEIEBE_00004 4513 6 Skin 0.29 protein_coding synonymous_variant LOW 276A>G Glu92Glu
M0083716 NHJEIEBE_00003 2405 4 Skin 0.19 protein_coding synonymous_variant LOW 1068A>G Gly356Gly
M0083717 NHJEIEBE_00002 3797 4 Skin 0.19 protein_coding upstream_gene_variant MODIFIER -1487T>C None
M0083718 NHJEIEBE_00002 3801 3 Skin 0.14 protein_coding upstream_gene_variant MODIFIER -1491G>A None
M0083719 NHJEIEBE_00002 3898 5 Skin 0.24 protein_coding upstream_gene_variant MODIFIER -1588A>T None
M0083720 NHJEIEBE_00002 3919 6 Skin 0.29 protein_coding upstream_gene_variant MODIFIER -1609C>T None
M0083721 NHJEIEBE_00002 3921 6 Skin 0.29 protein_coding upstream_gene_variant MODIFIER -1611G>T None
M0083722 NHJEIEBE_00002 3928 3 Skin 0.14 protein_coding upstream_gene_variant MODIFIER -1618G>A None
M0083723 NHJEIEBE_00002 3943 5 Skin 0.24 protein_coding upstream_gene_variant MODIFIER -1633C>T None
M0083724 NHJEIEBE_00004 4132 4 Skin 0.19 protein_coding synonymous_variant LOW 657T>C Val219Val
M0083725 NHJEIEBE_00004 4360 3 Skin 0.14 protein_coding synonymous_variant LOW 429T>G Arg143Arg






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term