Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C3324
  Reference Plasmid   MGYG000298413__MGYG000298413_8
  Reference Plasmid Size   285638
  Reference Plasmid GC Content   0.48
  Reference Plasmid Mobility Type   non-mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0135373 JGOHBKEC_00073 65914 4 Oral 0.44 protein_coding missense_variant MODERATE 650T>C Ile217Thr
M0135374 JGOHBKEC_00073 66150 4 Oral 0.44 protein_coding synonymous_variant LOW 414A>G Arg138Arg
M0135375 JGOHBKEC_00074 66905 3 Oral 0.33 protein_coding missense_variant MODERATE 170A>G Asn57Ser
M0135376 JGOHBKEC_00074 66994 4 Oral 0.44 protein_coding synonymous_variant LOW 81G>A Leu27Leu
M0135377 JGOHBKEC_00074 67015 4 Oral 0.44 protein_coding synonymous_variant LOW 60G>A Lys20Lys
M0135378 JGOHBKEC_00076 67876 3 Oral 0.33 protein_coding synonymous_variant LOW 663A>G Leu221Leu
M0135379 JGOHBKEC_00076 67878 3 Oral 0.33 protein_coding synonymous_variant LOW 661T>C Leu221Leu
M0135380 JGOHBKEC_00076 68125 3 Oral 0.33 protein_coding missense_variant MODERATE 414T>A His138Gln
M0135381 JGOHBKEC_00076 68164 3 Oral 0.33 protein_coding synonymous_variant LOW 375A>G Ala125Ala
M0135382 JGOHBKEC_00076 68289 3 Oral 0.33 protein_coding missense_variant MODERATE 250A>G Met84Val
M0135383 JGOHBKEC_00076 68305 3 Oral 0.33 protein_coding synonymous_variant LOW 234T>C His78His
M0135384 JGOHBKEC_00076 68310 3 Oral 0.33 protein_coding missense_variant MODERATE 229G>A Ala77Thr
M0135385 JGOHBKEC_00076 68326 3 Oral 0.33 protein_coding synonymous_variant LOW 213T>C Ala71Ala
M0135386 JGOHBKEC_00076 68358 3 Oral 0.33 protein_coding missense_variant MODERATE 181T>A Cys61Ser
M0135387 JGOHBKEC_00076 68385 3 Oral 0.33 protein_coding missense_variant MODERATE 154A>G Thr52Ala
M0135388 JGOHBKEC_00076 68482 3 Oral 0.33 protein_coding synonymous_variant LOW 57G>A Ala19Ala
M0135389 JGOHBKEC_00076 68533 3 Oral 0.33 protein_coding synonymous_variant LOW 6T>C Ser2Ser
M0135390 JGOHBKEC_00077 68583 3 Oral 0.33 protein_coding synonymous_variant LOW 1185G>A Lys395Lys
M0135391 JGOHBKEC_00077 68646 3 Oral 0.33 protein_coding synonymous_variant LOW 1122C>T Arg374Arg
M0135392 JGOHBKEC_00077 68661 3 Oral 0.33 protein_coding synonymous_variant LOW 1107A>G Leu369Leu
M0135393 JGOHBKEC_00077 68751 3 Oral 0.33 protein_coding synonymous_variant LOW 1017G>A Gly339Gly
M0135394 JGOHBKEC_00077 68835 3 Oral 0.33 protein_coding synonymous_variant LOW 933A>G Ala311Ala
M0135395 JGOHBKEC_00077 68910 3 Oral 0.33 protein_coding synonymous_variant LOW 858C>T Asn286Asn
M0135396 JGOHBKEC_00077 69015 3 Oral 0.33 protein_coding synonymous_variant LOW 753C>T Ser251Ser
M0135397 JGOHBKEC_00077 69078 3 Oral 0.33 protein_coding synonymous_variant LOW 690T>C Ala230Ala
M0135398 JGOHBKEC_00077 69381 3 Oral 0.33 protein_coding synonymous_variant LOW 387T>C Asp129Asp
M0135399 JGOHBKEC_00077 69429 3 Oral 0.33 protein_coding synonymous_variant LOW 339G>A Gly113Gly
M0135400 JGOHBKEC_00077 69450 3 Oral 0.33 protein_coding synonymous_variant LOW 318A>G Leu106Leu
M0135401 JGOHBKEC_00077 69468 3 Oral 0.33 protein_coding synonymous_variant LOW 300A>G Gly100Gly
M0135402 JGOHBKEC_00077 69483 3 Oral 0.33 protein_coding synonymous_variant LOW 285A>G Lys95Lys
M0135403 JGOHBKEC_00077 69515 3 Oral 0.33 protein_coding synonymous_variant LOW 253T>C Leu85Leu
M0135404 JGOHBKEC_00077 69547 3 Oral 0.33 protein_coding missense_variant MODERATE 221C>T Ser74Leu
M0135405 JGOHBKEC_00077 69570 3 Oral 0.33 protein_coding synonymous_variant LOW 198A>G Leu66Leu
M0135406 JGOHBKEC_00077 69591 3 Oral 0.33 protein_coding synonymous_variant LOW 177T>C Val59Val
M0135407 JGOHBKEC_00077 69617 3 Oral 0.33 protein_coding synonymous_variant LOW 151C>T Leu51Leu
M0135408 JGOHBKEC_00077 69633 3 Oral 0.33 protein_coding synonymous_variant LOW 135T>C Pro45Pro
M0135409 JGOHBKEC_00074 66651 3 Oral 0.33 protein_coding missense_variant MODERATE 424A>G Thr142Ala
M0135410 JGOHBKEC_00074 66663 3 Oral 0.33 protein_coding missense_variant MODERATE 412A>G Thr138Ala
M0135411 JGOHBKEC_00074 66679 3 Oral 0.33 protein_coding synonymous_variant LOW 396T>C Thr132Thr
M0135412 JGOHBKEC_00074 66758 3 Oral 0.33 protein_coding missense_variant MODERATE 317G>A Arg106Gln
M0135413 JGOHBKEC_00074 66847 3 Oral 0.33 protein_coding synonymous_variant LOW 228C>T Asn76Asn
M0135414 JGOHBKEC_00075 67309 3 Oral 0.33 protein_coding synonymous_variant LOW 483G>A Val161Val
M0135415 JGOHBKEC_00029 28263 3 Oral 0.33 protein_coding missense_variant MODERATE 475A>G Ile159Val
M0135416 JGOHBKEC_00029 28429 3 Oral 0.33 protein_coding synonymous_variant LOW 309C>T Val103Val
M0135417 JGOHBKEC_00029 28477 3 Oral 0.33 protein_coding synonymous_variant LOW 261A>G Lys87Lys
M0135418 JGOHBKEC_00029 28718 3 Oral 0.33 protein_coding missense_variant MODERATE 20A>G Tyr7Cys
M0135419 JGOHBKEC_00029 28736 3 Oral 0.33 protein_coding start_lost HIGH 2T>A Leu1?
M0135420 JGOHBKEC_00027 28748 3 Oral 0.33 protein_coding upstream_gene_variant MODIFIER -1335A>G None






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
JGOHBKEC_00045 VEH14211.1|GH92 88 0 1 719 1 0.9958
JGOHBKEC_00046 VEH14212.1|GH33 86.1 1.21e-261 1 396 1 0.7059
JGOHBKEC_00050 VEH14214.1|GH92 92.1 0 1 717 0.9755 0.9547
JGOHBKEC_00247 QUB83811.1|GH97 70.1 3.76e-290 1 521 1 0.7738





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
JGOHBKEC_00164 1.B.57.4.6 70.1 3.6e-160 2 386 0.9974 0.9820 1 Channels/Pores 1.B β-Barrel Porins 1.B.57 The Legionella Major-Outer Membrane Protein (LM-OMP) Family