Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C282
  Reference Plasmid   NC_006135.1
  Reference Plasmid Size   45205
  Reference Plasmid GC Content   0.29
  Reference Plasmid Mobility Type   conjugative





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0151149 AEKHKHCJ_00002 2331 7 Gut 0.64 protein_coding upstream_gene_variant MODIFIER -18T>C None
M0151150 AEKHKHCJ_00002 2555 8 Gut 0.73 protein_coding synonymous_variant LOW 207G>A Gly69Gly
M0151151 AEKHKHCJ_00002 2576 4 Gut 0.36 protein_coding synonymous_variant LOW 228C>T His76His
M0151152 AEKHKHCJ_00002 2675 4 Gut 0.36 protein_coding synonymous_variant LOW 327T>C Phe109Phe
M0151153 AEKHKHCJ_00002 2687 6 Gut 0.55 protein_coding synonymous_variant LOW 339T>C Ser113Ser
M0151154 AEKHKHCJ_00002 2868 8 Gut 0.73 protein_coding missense_variant MODERATE 520G>A Glu174Lys
M0151155 AEKHKHCJ_00002 2946 4 Gut 0.36 protein_coding missense_variant MODERATE 598G>A Ala200Thr
M0151156 AEKHKHCJ_00002 3001 7 Gut 0.64 protein_coding missense_variant MODERATE 653C>T Pro218Leu
M0151157 AEKHKHCJ_00003 3024 4 Gut 0.36 protein_coding upstream_gene_variant MODIFIER -282C>T None
M0151158 AEKHKHCJ_00004 3824 3 Gut 0.27 protein_coding upstream_gene_variant MODIFIER -97T>C None
M0151159 AEKHKHCJ_00004 3912 4 Gut 0.36 protein_coding upstream_gene_variant MODIFIER -9C>A None
M0151160 AEKHKHCJ_00004 4094 4 Gut 0.36 protein_coding synonymous_variant LOW 174T>C Gly58Gly
M0151161 AEKHKHCJ_00004 4124 4 Gut 0.36 protein_coding synonymous_variant LOW 204T>C Ser68Ser
M0151162 AEKHKHCJ_00004 4145 4 Gut 0.36 protein_coding synonymous_variant LOW 225G>A Gln75Gln
M0151163 AEKHKHCJ_00004 4190 4 Gut 0.36 protein_coding synonymous_variant LOW 270A>G Gly90Gly
M0151164 AEKHKHCJ_00002 2304 3 Gut 0.27 protein_coding upstream_gene_variant MODIFIER -45T>C None
M0151165 AEKHKHCJ_00002 2879 3 Gut 0.27 protein_coding synonymous_variant LOW 531A>G Ala177Ala
M0151166 AEKHKHCJ_00002 2992 4 Gut 0.36 protein_coding missense_variant MODERATE 644A>G Asp215Gly
M0151167 AEKHKHCJ_00002 3000 3 Gut 0.27 protein_coding missense_variant MODERATE 652C>T Pro218Ser
M0151168 AEKHKHCJ_00003 3048 3 Gut 0.27 protein_coding upstream_gene_variant MODIFIER -258G>A None
M0151169 AEKHKHCJ_00004 3815 3 Gut 0.27 protein_coding upstream_gene_variant MODIFIER -106A>G None
M0151170 AEKHKHCJ_00004 3910 3 Gut 0.27 protein_coding upstream_gene_variant MODIFIER -11T>G None
M0151171 AEKHKHCJ_00004 3932 3 Gut 0.27 protein_coding synonymous_variant LOW 12C>T Tyr4Tyr
M0151172 AEKHKHCJ_00004 3954 3 Gut 0.27 protein_coding missense_variant MODERATE 34G>A Gly12Arg
M0151173 AEKHKHCJ_00004 4280 3 Gut 0.27 protein_coding synonymous_variant LOW 360C>T Asp120Asp
M0151174 AEKHKHCJ_00004 4286 3 Gut 0.27 protein_coding synonymous_variant LOW 366T>C Asp122Asp
M0151175 AEKHKHCJ_00004 4295 3 Gut 0.27 protein_coding synonymous_variant LOW 375C>T Gly125Gly
M0151176 AEKHKHCJ_00004 4337 3 Gut 0.27 protein_coding synonymous_variant LOW 417T>C Gly139Gly
M0151177 AEKHKHCJ_00004 4340 3 Gut 0.27 protein_coding synonymous_variant LOW 420G>A Val140Val
M0151178 AEKHKHCJ_00004 4379 3 Gut 0.27 protein_coding synonymous_variant LOW 459G>C Gly153Gly
M0151179 AEKHKHCJ_00004 4445 3 Gut 0.27 protein_coding synonymous_variant LOW 525G>A Ala175Ala
M0151180 AEKHKHCJ_00004 4451 3 Gut 0.27 protein_coding synonymous_variant LOW 531G>A Leu177Leu
M0151181 AEKHKHCJ_00004 4460 3 Gut 0.27 protein_coding synonymous_variant LOW 540C>T Ala180Ala
M0151182 AEKHKHCJ_00004 4461 3 Gut 0.27 protein_coding missense_variant MODERATE 541T>C Phe181Leu
M0151183 AEKHKHCJ_00004 4466 4 Gut 0.36 protein_coding synonymous_variant LOW 546T>C Ile182Ile
M0151184 AEKHKHCJ_00004 4496 3 Gut 0.27 protein_coding synonymous_variant LOW 576C>T Ile192Ile
M0151185 AEKHKHCJ_00004 4523 3 Gut 0.27 protein_coding synonymous_variant LOW 603A>C Gly201Gly
M0151186 AEKHKHCJ_00004 4538 3 Gut 0.27 protein_coding synonymous_variant LOW 618C>T Phe206Phe
M0151187 AEKHKHCJ_00004 4550 3 Gut 0.27 protein_coding synonymous_variant LOW 630G>C Gly210Gly
M0151188 AEKHKHCJ_00004 4568 3 Gut 0.27 protein_coding synonymous_variant LOW 648T>G Gly216Gly
M0151189 AEKHKHCJ_00004 4570 4 Gut 0.36 protein_coding missense_variant MODERATE 650T>C Val217Ala
M0151190 AEKHKHCJ_00004 4577 3 Gut 0.27 protein_coding synonymous_variant LOW 657C>T Phe219Phe
M0151191 AEKHKHCJ_00004 4596 3 Gut 0.27 protein_coding synonymous_variant LOW 676C>T Leu226Leu
M0151192 AEKHKHCJ_00004 4625 3 Gut 0.27 protein_coding synonymous_variant LOW 705C>T Asp235Asp
M0151193 AEKHKHCJ_00004 4646 3 Gut 0.27 protein_coding synonymous_variant LOW 726C>T Ser242Ser
M0151194 AEKHKHCJ_00004 4655 3 Gut 0.27 protein_coding synonymous_variant LOW 735A>T Val245Val
M0151195 AEKHKHCJ_00004 4659 3 Gut 0.27 protein_coding missense_variant MODERATE 739C>T Leu247Phe
M0151196 AEKHKHCJ_00004 4694 3 Gut 0.27 protein_coding synonymous_variant LOW 774T>C Ser258Ser
M0151197 AEKHKHCJ_00004 4722 3 Gut 0.27 protein_coding synonymous_variant LOW 802C>T Leu268Leu
M0151198 AEKHKHCJ_00004 4733 3 Gut 0.27 protein_coding missense_variant MODERATE 813A>T Glu271Asp
M0151199 AEKHKHCJ_00004 4781 3 Gut 0.27 protein_coding synonymous_variant LOW 861G>A Lys287Lys
M0151200 AEKHKHCJ_00004 4802 3 Gut 0.27 protein_coding synonymous_variant LOW 882A>G Val294Val
M0151201 AEKHKHCJ_00004 4844 3 Gut 0.27 protein_coding synonymous_variant LOW 924C>A Thr308Thr
M0151202 AEKHKHCJ_00004 4853 3 Gut 0.27 protein_coding synonymous_variant LOW 933G>A Val311Val
M0151203 AEKHKHCJ_00004 4865 3 Gut 0.27 protein_coding synonymous_variant LOW 945G>A Gly315Gly
M0151204 AEKHKHCJ_00004 4877 3 Gut 0.27 protein_coding synonymous_variant LOW 957T>C Ser319Ser
M0151205 AEKHKHCJ_00004 4886 3 Gut 0.27 protein_coding synonymous_variant LOW 966G>A Ala322Ala
M0151206 AEKHKHCJ_00004 4889 3 Gut 0.27 protein_coding synonymous_variant LOW 969G>A Leu323Leu
M0151207 AEKHKHCJ_00002 2492 5 Gut 0.45 protein_coding synonymous_variant LOW 144T>C Asp48Asp
M0151208 AEKHKHCJ_00002 2521 3 Gut 0.27 protein_coding missense_variant MODERATE 173C>A Thr58Asn
M0151209 AEKHKHCJ_00002 2532 3 Gut 0.27 protein_coding missense_variant MODERATE 184C>A Leu62Met
M0151210 AEKHKHCJ_00002 2558 3 Gut 0.27 protein_coding synonymous_variant LOW 210G>A Val70Val






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism
AEKHKHCJ_00001 ARO:3000190 99.8 0 1 639 1.0000 1.0000 tetracycline antibiotic tetracycline-resistant ribosomal protection protein antibiotic target protection






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term