Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C320
  Reference Plasmid   NC_015423.1
  Reference Plasmid Size   75488
  Reference Plasmid GC Content   0.62
  Reference Plasmid Mobility Type   conjugative





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0084666 EHFCPMHH_00070 56052 7 Skin 0.88 protein_coding missense_variant MODERATE 1366A>G Lys456Glu
M0084667 EHFCPMHH_00070 56269 7 Skin 0.88 protein_coding synonymous_variant LOW 1149C>G Arg383Arg
M0084668 EHFCPMHH_00070 56729 7 Skin 0.88 protein_coding missense_variant MODERATE 689T>C Ile230Thr
M0084669 EHFCPMHH_00071 58250 6 Skin 0.75 protein_coding missense_variant MODERATE 2328G>T Met776Ile
M0084670 EHFCPMHH_00071 58257 6 Skin 0.75 protein_coding missense_variant MODERATE 2321G>A Arg774Gln
M0084671 EHFCPMHH_00071 58710 6 Skin 0.75 protein_coding missense_variant MODERATE 1868C>G Ala623Gly
M0084672 EHFCPMHH_00072 60639 6 Skin 0.75 protein_coding synonymous_variant LOW 1224C>T Ile408Ile
M0084673 EHFCPMHH_00072 60924 6 Skin 0.75 protein_coding synonymous_variant LOW 939C>T Asn313Asn
M0084674 EHFCPMHH_00072 61120 6 Skin 0.75 protein_coding missense_variant MODERATE 743G>T Ser248Ile
M0084675 EHFCPMHH_00072 61121 6 Skin 0.75 protein_coding missense_variant MODERATE 742A>G Ser248Gly
M0084676 EHFCPMHH_00072 61583 6 Skin 0.75 protein_coding missense_variant MODERATE 280A>G Ser94Gly
M0084677 EHFCPMHH_00072 61621 6 Skin 0.75 protein_coding missense_variant MODERATE 242T>A Leu81Gln
M0084678 EHFCPMHH_00072 61710 6 Skin 0.75 protein_coding synonymous_variant LOW 153G>A Leu51Leu
M0084679 EHFCPMHH_00070 61911 6 Skin 0.75 protein_coding upstream_gene_variant MODIFIER -4494C>A None
M0084680 EHFCPMHH_00073 62021 6 Skin 0.75 protein_coding synonymous_variant LOW 84G>A Gly28Gly
M0084681 EHFCPMHH_00073 62299 6 Skin 0.75 protein_coding missense_variant MODERATE 362C>A Pro121Gln
M0084682 EHFCPMHH_00073 62305 6 Skin 0.75 protein_coding missense_variant MODERATE 368C>T Pro123Leu
M0084683 EHFCPMHH_00073 62307 6 Skin 0.75 protein_coding missense_variant MODERATE 370A>T Ser124Cys
M0084684 EHFCPMHH_00073 62330 6 Skin 0.75 protein_coding synonymous_variant LOW 393T>C Asn131Asn
M0084685 EHFCPMHH_00073 62332 6 Skin 0.75 protein_coding missense_variant MODERATE 395T>C Met132Thr
M0084686 EHFCPMHH_00073 62385 6 Skin 0.75 protein_coding missense_variant MODERATE 448T>C Ser150Pro
M0084687 EHFCPMHH_00073 62410 6 Skin 0.75 protein_coding missense_variant MODERATE 473G>A Ser158Asn
M0084688 EHFCPMHH_00073 62470 6 Skin 0.75 protein_coding missense_variant MODERATE 533T>C Phe178Ser
M0084689 EHFCPMHH_00073 62538 6 Skin 0.75 protein_coding missense_variant MODERATE 601T>C Ser201Pro






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group
EHFCPMHH_00071 Benzylkonium Chloride (BAC) [class: Quaternary Ammonium Compounds (QACs)], Clorine Dioxide (ClO2) [class: Halogens], Triclosan [class: Phenolic compounds], Cetrimide (CTM) [class: Quaternary Ammonium Compounds (QACs)], Chlorhexidine [class: Biguanides], Sodium Dodecyl Sulfate (SDS) [class: Organo-sulfate], Acriflavine [class: Acridine] 72.5 0 1 1041 0.9867 0.9914 experiment
EHFCPMHH_00071 Benzylkonium Chloride (BAC) [class: Quaternary Ammonium Compounds (QACs)], Clorine Dioxide (ClO2) [class: Halogens], Triclosan [class: Phenolic compounds], Cetrimide (CTM) [class: Quaternary Ammonium Compounds (QACs)], Chlorhexidine [class: Biguanides], Sodium Dodecyl Sulfate (SDS) [class: Organo-sulfate], Acriflavine [class: Acridine] 72.7 0 1 1041 0.9867 0.9914 prediction






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism
EHFCPMHH_00071 ARO:3003923 72.5 0 1 1041 0.9867 0.9914 fluoroquinolone antibiotic resistance-nodulation-cell division (RND) antibiotic efflux pump antibiotic efflux






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant
EHFCPMHH_00071 PHI:6449 oqxB 72.6 0 1 1042 0.9877 0.9933 nematodes pneumonia multidrug efflux pump reduced virulence






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
EHFCPMHH_00010 AEB87057.1|GH23 100 7.36e-120 1 166 1 1





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
EHFCPMHH_00071 2.A.6.2.47 72.6 0 1 1041 0.9867 0.9914 2 Electrochemical Potential-driven Transporters 2.A Porters (uniporters, symporters, antiporters) 2.A.6 The Resistance-Nodulation-Cell Division (RND) Superfamily