Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C324
  Reference Plasmid   NC_017315.1
  Reference Plasmid Size   55393
  Reference Plasmid GC Content   0.35
  Reference Plasmid Mobility Type   mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0153075 NADALAIB_00031 27778 6 Gut 0.75 protein_coding missense_variant MODERATE 529G>T Ala177Ser
M0153076 NADALAIB_00031 27830 6 Gut 0.75 protein_coding missense_variant MODERATE 477C>A Asn159Lys
M0153077 NADALAIB_00031 27831 6 Gut 0.75 protein_coding missense_variant MODERATE 476A>C Asn159Thr
M0153078 NADALAIB_00031 27905 6 Gut 0.75 protein_coding missense_variant MODERATE 402A>C Leu134Phe
M0153079 NADALAIB_00023 28307 6 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -4500T>A None
M0153080 NADALAIB_00023 28309 6 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -4502G>A None
M0153081 NADALAIB_00023 28329 6 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -4522G>A None
M0153082 NADALAIB_00032 28333 6 Gut 0.75 protein_coding missense_variant MODERATE 450A>T Gln150His
M0153083 NADALAIB_00032 28340 6 Gut 0.75 protein_coding missense_variant MODERATE 443T>C Val148Ala
M0153084 NADALAIB_00032 28342 6 Gut 0.75 protein_coding synonymous_variant LOW 441A>T Pro147Pro
M0153085 NADALAIB_00032 28357 6 Gut 0.75 protein_coding missense_variant MODERATE 426G>T Glu142Asp
M0153086 NADALAIB_00032 28444 6 Gut 0.75 protein_coding synonymous_variant LOW 339C>T Phe113Phe
M0153087 NADALAIB_00032 28624 7 Gut 0.88 protein_coding synonymous_variant LOW 159A>G Gly53Gly
M0153088 NADALAIB_00032 28627 7 Gut 0.88 protein_coding missense_variant MODERATE 156C>A Asp52Glu
M0153089 NADALAIB_00032 28645 7 Gut 0.88 protein_coding synonymous_variant LOW 138G>A Arg46Arg
M0153090 NADALAIB_00023 28789 7 Gut 0.88 protein_coding upstream_gene_variant MODIFIER -4982A>T None
M0153091 NADALAIB_00023 28790 7 Gut 0.88 protein_coding upstream_gene_variant MODIFIER -4983A>T None
M0153092 NADALAIB_00023 28791 7 Gut 0.88 protein_coding upstream_gene_variant MODIFIER -4984A>C None
M0153093 NADALAIB_00024 28810 7 Gut 0.88 protein_coding upstream_gene_variant MODIFIER -4754G>A None
M0153094 NADALAIB_00034 29521 8 Gut 1.00 protein_coding synonymous_variant LOW 63C>T Ala21Ala
M0153095 NADALAIB_00026 29605 8 Gut 1.00 protein_coding upstream_gene_variant MODIFIER -4745A>G None
M0153096 NADALAIB_00035 29649 8 Gut 1.00 protein_coding missense_variant MODERATE 234T>A Asn78Lys
M0153097 NADALAIB_00035 29670 8 Gut 1.00 protein_coding synonymous_variant LOW 213C>A Ala71Ala
M0153098 NADALAIB_00035 29693 8 Gut 1.00 protein_coding missense_variant MODERATE 190T>A Leu64Ile
M0153099 NADALAIB_00035 29853 8 Gut 1.00 protein_coding missense_variant MODERATE 30C>A Phe10Leu
M0153100 NADALAIB_00027 30147 8 Gut 1.00 protein_coding upstream_gene_variant MODIFIER -4791T>C None
M0153101 NADALAIB_00027 30148 8 Gut 1.00 protein_coding upstream_gene_variant MODIFIER -4792T>A None
M0153102 NADALAIB_00027 30149 8 Gut 1.00 protein_coding upstream_gene_variant MODIFIER -4793G>A None
M0153103 NADALAIB_00027 30331 8 Gut 1.00 protein_coding upstream_gene_variant MODIFIER -4975G>T None
M0153104 NADALAIB_00036 30873 8 Gut 1.00 protein_coding synonymous_variant LOW 90A>G Gly30Gly
M0153105 NADALAIB_00036 30894 8 Gut 1.00 protein_coding synonymous_variant LOW 69C>A Gly23Gly
M0153106 NADALAIB_00036 30923 8 Gut 1.00 protein_coding missense_variant MODERATE 40G>A Gly14Ser
M0153107 NADALAIB_00029 31993 5 Gut 0.63 protein_coding upstream_gene_variant MODIFIER -4653A>T None
M0153108 NADALAIB_00029 32214 4 Gut 0.50 protein_coding upstream_gene_variant MODIFIER -4874G>T None
M0153109 NADALAIB_00038 32971 4 Gut 0.50 protein_coding synonymous_variant LOW 1564C>T Leu522Leu
M0153110 NADALAIB_00038 33125 4 Gut 0.50 protein_coding synonymous_variant LOW 1410A>C Leu470Leu
M0153111 NADALAIB_00038 33380 4 Gut 0.50 protein_coding synonymous_variant LOW 1155G>A Ala385Ala
M0153112 NADALAIB_00038 34064 4 Gut 0.50 protein_coding synonymous_variant LOW 471G>T Val157Val
M0153113 NADALAIB_00038 34109 4 Gut 0.50 protein_coding synonymous_variant LOW 426A>G Gly142Gly
M0153114 NADALAIB_00038 34118 4 Gut 0.50 protein_coding synonymous_variant LOW 417C>T Ser139Ser
M0153115 NADALAIB_00038 34163 4 Gut 0.50 protein_coding synonymous_variant LOW 372G>T Leu124Leu
M0153116 NADALAIB_00039 35166 3 Gut 0.38 protein_coding synonymous_variant LOW 1701G>A Lys567Lys
M0153117 NADALAIB_00039 35265 3 Gut 0.38 protein_coding synonymous_variant LOW 1602G>A Glu534Glu
M0153118 NADALAIB_00039 35271 3 Gut 0.38 protein_coding synonymous_variant LOW 1596T>C Arg532Arg
M0153119 NADALAIB_00039 35277 3 Gut 0.38 protein_coding synonymous_variant LOW 1590G>A Ala530Ala
M0153120 NADALAIB_00039 35310 3 Gut 0.38 protein_coding synonymous_variant LOW 1557A>G Arg519Arg
M0153121 NADALAIB_00039 35424 3 Gut 0.38 protein_coding synonymous_variant LOW 1443A>G Lys481Lys
M0153122 NADALAIB_00029 27076 3 Gut 0.38 protein_coding synonymous_variant LOW 265C>T Leu89Leu
M0153123 NADALAIB_00029 27230 4 Gut 0.50 protein_coding synonymous_variant LOW 111A>G Gln37Gln
M0153124 NADALAIB_00022 27629 4 Gut 0.50 protein_coding upstream_gene_variant MODIFIER -4908T>C None
M0153125 NADALAIB_00022 27658 4 Gut 0.50 protein_coding upstream_gene_variant MODIFIER -4937T>A None
M0153126 NADALAIB_00022 27659 4 Gut 0.50 protein_coding upstream_gene_variant MODIFIER -4938A>G None
M0153127 NADALAIB_00022 27660 4 Gut 0.50 protein_coding upstream_gene_variant MODIFIER -4939T>C None
M0153128 NADALAIB_00022 27676 4 Gut 0.50 protein_coding upstream_gene_variant MODIFIER -4955C>T None
M0153129 NADALAIB_00022 27678 4 Gut 0.50 protein_coding upstream_gene_variant MODIFIER -4957C>T None






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition
NADALAIB_00057 VFG002164 AS 95.9 0 1 1305 1.0 1 Adherence aggregation substance PrgB/Asc10 experiment
NADALAIB_00057 VFG002164 AS 95.9 0 1 1305 1.0 1 Adherence aggregation substance PrgB/Asc10 prediction







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant
NADALAIB_00036 PHI:2999 EF0026 99.4 5.1e-92 1 162 1.0000 1.0000 moths nosocomial infection None increased virulence (hypervirulence)






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
NADALAIB_00051 ADX81317.1|GH73 100 6.60000000000865e-313 1 423 1 1





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term