Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C331
  Reference Plasmid   NC_017722.1
  Reference Plasmid Size   66681
  Reference Plasmid GC Content   0.51
  Reference Plasmid Mobility Type   conjugative





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0153196 JAKBCPAC_00076 59427 6 Gut 0.43 protein_coding stop_gained HIGH 265C>T Arg89*
M0153197 JAKBCPAC_00073 59501 6 Gut 0.43 protein_coding upstream_gene_variant MODIFIER -2232T>C None
M0153198 JAKBCPAC_00077 59856 6 Gut 0.43 protein_coding missense_variant MODERATE 1246C>T His416Tyr
M0153199 JAKBCPAC_00077 60078 6 Gut 0.43 protein_coding synonymous_variant LOW 1024C>T Leu342Leu
M0153200 JAKBCPAC_00077 60951 5 Gut 0.36 protein_coding missense_variant MODERATE 151C>A Gln51Lys
M0153201 JAKBCPAC_00078 61365 6 Gut 0.43 protein_coding synonymous_variant LOW 456T>C Arg152Arg
M0153202 JAKBCPAC_00078 61710 5 Gut 0.36 protein_coding synonymous_variant LOW 111C>A Gly37Gly
M0153203 JAKBCPAC_00073 61859 6 Gut 0.43 protein_coding upstream_gene_variant MODIFIER -4590G>A None
M0153204 JAKBCPAC_00073 61860 6 Gut 0.43 protein_coding upstream_gene_variant MODIFIER -4591C>T None
M0153205 JAKBCPAC_00079 62387 6 Gut 0.43 protein_coding synonymous_variant LOW 426A>G Leu142Leu
M0153206 JAKBCPAC_00079 62390 6 Gut 0.43 protein_coding synonymous_variant LOW 429T>C Val143Val
M0153207 JAKBCPAC_00079 62432 6 Gut 0.43 protein_coding synonymous_variant LOW 471C>A Leu157Leu
M0153208 JAKBCPAC_00079 62447 6 Gut 0.43 protein_coding synonymous_variant LOW 486G>A Ala162Ala
M0153209 JAKBCPAC_00080 62619 6 Gut 0.43 protein_coding synonymous_variant LOW 48A>G Val16Val
M0153210 JAKBCPAC_00080 62797 6 Gut 0.43 protein_coding missense_variant MODERATE 226C>T His76Tyr
M0153211 JAKBCPAC_00080 62865 6 Gut 0.43 protein_coding synonymous_variant LOW 294T>G Gly98Gly
M0153212 JAKBCPAC_00080 63102 6 Gut 0.43 protein_coding synonymous_variant LOW 531C>T Arg177Arg
M0153213 JAKBCPAC_00080 63153 5 Gut 0.36 protein_coding synonymous_variant LOW 582A>G Pro194Pro
M0153214 JAKBCPAC_00081 63524 6 Gut 0.43 protein_coding synonymous_variant LOW 153T>C His51His
M0153215 JAKBCPAC_00077 60915 3 Gut 0.21 protein_coding missense_variant MODERATE 187A>T Thr63Ser






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition
JAKBCPAC_00064 VFG036068 Heat-labile toxin (LT) 100 4.8e-67 1 124 1.0 1 Exotoxin enterotoxin subunit B (from human) experiment
JAKBCPAC_00065 VFG036066 Heat-labile toxin (LT) 100 1.4e-130 1 218 1.0 0.845 Exotoxin heat-labile enterotoxin A prepeptide (from human) experiment
JAKBCPAC_00073 VFG002039 Heat-stable toxin (ST) 100 7.4e-36 1 72 1.0 1 Exotoxin heat stable enterotoxin I experiment
JAKBCPAC_00064 VFG036068 Heat-labile toxin (LT) 100 3.6e-66 1 124 1.0 1 Exotoxin enterotoxin subunit B (from human) prediction
JAKBCPAC_00065 VFG036066 Heat-labile toxin (LT) 100 1.1e-129 1 218 1.0 0.845 Exotoxin heat-labile enterotoxin A prepeptide (from human) prediction
JAKBCPAC_00073 VFG002039 Heat-stable toxin (ST) 100 5.5e-35 1 72 1.0 1 Exotoxin heat stable enterotoxin I prediction







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant
JAKBCPAC_00064 PHI:699 CtxB 78.2 1.9e-51 1 124 1.0000 1.0000 primates cholera Cholera toxin (CT) reduced virulence
JAKBCPAC_00065 PHI:698 CtxA 80.3 1.1e-105 1 218 1.0000 0.8450 primates cholera Cholera toxin (CT) reduced virulence






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
JAKBCPAC_00036 CBJ04400.1|GH23 100 8.23e-123 1 169 1 1





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
JAKBCPAC_00065 1.C.72.4.1 100 8.3e-130 1 218 1.0000 0.8450 1 Channels/Pores 1.C Pore-Forming Toxins (Proteins and Peptides) 1.C.72 The Pertussis Toxin (PTX) Family