Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C341
  Reference Plasmid   NC_021077.1
  Reference Plasmid Size   119225
  Reference Plasmid GC Content   0.64
  Reference Plasmid Mobility Type   conjugative





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0085514 CNNGAMKA_00023 22805 3 Skin 0.38 protein_coding synonymous_variant LOW 339T>C Val113Val
M0085515 CNNGAMKA_00023 22811 3 Skin 0.38 protein_coding synonymous_variant LOW 333C>T Asp111Asp
M0085516 CNNGAMKA_00023 22815 3 Skin 0.38 protein_coding missense_variant MODERATE 329G>A Ser110Asn
M0085517 CNNGAMKA_00023 22823 3 Skin 0.38 protein_coding synonymous_variant LOW 321A>G Ala107Ala
M0085518 CNNGAMKA_00023 22826 3 Skin 0.38 protein_coding synonymous_variant LOW 318G>A Ala106Ala
M0085519 CNNGAMKA_00023 22829 3 Skin 0.38 protein_coding synonymous_variant LOW 315G>A Thr105Thr
M0085520 CNNGAMKA_00023 22841 3 Skin 0.38 protein_coding synonymous_variant LOW 303G>C Arg101Arg
M0085521 CNNGAMKA_00024 23273 3 Skin 0.38 protein_coding synonymous_variant LOW 1182C>T Asp394Asp
M0085522 CNNGAMKA_00024 23330 3 Skin 0.38 protein_coding synonymous_variant LOW 1125C>G Pro375Pro
M0085523 CNNGAMKA_00024 23333 3 Skin 0.38 protein_coding synonymous_variant LOW 1122C>T His374His






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition
CNNGAMKA_00120 VFG045767 Lvh (Legionella vir homologs) type IVA secretion system 70.5 4.3e-124 1 315 0.9844 0.9813 Effector delivery system P-type conjugative transfer ATPase TrbB prediction







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group
CNNGAMKA_00002 Mercury (Hg) 100 7.1e-78 1 144 1.0000 1.0000 experiment
CNNGAMKA_00003 Mercury (Hg) 100 4.4e-62 1 116 1.0000 1.0000 experiment
CNNGAMKA_00004 Mercury (Hg) 100 2.7e-43 1 91 1.0000 1.0000 experiment
CNNGAMKA_00005 Mercury (Hg) 98.6 2.30000000001574e-313 1 561 1.0000 1.0000 experiment
CNNGAMKA_00006 Mercury (Hg) 100 1.9e-63 1 121 1.0000 1.0000 experiment
CNNGAMKA_00007 Mercury (Hg) 100 1.3e-41 1 78 1.0000 1.0000 experiment
CNNGAMKA_00002 Mercury (Hg) 100 1.6e-75 1 144 1.0000 1.0000 prediction
CNNGAMKA_00003 Mercury (Hg) 100 1e-59 1 116 1.0000 0.8657 prediction
CNNGAMKA_00004 Mercury (Hg) 98.9 1.1e-40 1 91 1.0000 1.0000 prediction
CNNGAMKA_00005 Mercury (Hg), Phenylmercury Acetate [class: Organo-mercury] 100 0 1 561 1.0000 1.0000 prediction
CNNGAMKA_00006 Mercury (Hg) 100 4.3e-61 1 121 1.0000 1.0000 prediction
CNNGAMKA_00007 Mercury (Hg) 100 3e-39 1 78 1.0000 1.0000 prediction
CNNGAMKA_00008 Mercury (Hg) 98.4 9.1e-186 1 315 0.9320 0.7702 prediction
CNNGAMKA_00038 Mercury (Hg) 100 7.7e-250 38 487 0.5338 0.7475 prediction
CNNGAMKA_00077 Mercury (Hg) 100 7.7e-250 38 487 0.5338 0.7475 prediction






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
CNNGAMKA_00108 QDL89612.1|GH23 100 7.37e-133 1 211 1 1





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
CNNGAMKA_00003 1.A.72.3.1 100 1.7e-60 1 116 1.0000 1.2747 1 Channels/Pores 1.A α-Type Channels 1.A.72 The Mercuric Ion Pore (Mer) Superfamily
CNNGAMKA_00004 1.A.72.3.1 100 1e-41 1 91 1.0000 1.0000 1 Channels/Pores 1.A α-Type Channels 1.A.72 The Mercuric Ion Pore (Mer) Superfamily
CNNGAMKA_00007 1.A.72.5.1 76 3e-29 1 75 0.9615 0.9615 1 Channels/Pores 1.A α-Type Channels 1.A.72 The Mercuric Ion Pore (Mer) Superfamily
CNNGAMKA_00110 3.A.7.4.1 99.7 7.6e-284 1 572 1.0000 1.7875 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.7 The Type IV (Conjugal DNA-Protein Transfer or VirB) Secretory Pathway (IVSP) Family
CNNGAMKA_00112 3.A.7.4.1 100 2.5e-130 1 254 1.0000 0.7938 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.7 The Type IV (Conjugal DNA-Protein Transfer or VirB) Secretory Pathway (IVSP) Family
CNNGAMKA_00113 3.A.7.4.1 99.6 2.5e-264 1 473 1.0000 1.4781 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.7 The Type IV (Conjugal DNA-Protein Transfer or VirB) Secretory Pathway (IVSP) Family
CNNGAMKA_00115 3.A.7.4.1 99.3 8.9e-167 1 299 1.0000 0.9344 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.7 The Type IV (Conjugal DNA-Protein Transfer or VirB) Secretory Pathway (IVSP) Family
CNNGAMKA_00116 3.A.7.4.1 99.2 1.1e-147 1 260 1.0000 0.8125 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.7 The Type IV (Conjugal DNA-Protein Transfer or VirB) Secretory Pathway (IVSP) Family
CNNGAMKA_00117 3.A.7.4.1 99.4 0 1 852 1.0000 2.6625 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.7 The Type IV (Conjugal DNA-Protein Transfer or VirB) Secretory Pathway (IVSP) Family
CNNGAMKA_00120 3.A.7.4.1 99.7 3.4e-180 1 320 1.0000 1.0000 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.7 The Type IV (Conjugal DNA-Protein Transfer or VirB) Secretory Pathway (IVSP) Family