Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C369
  Reference Plasmid   NZ_AP022816.1
  Reference Plasmid Size   114953
  Reference Plasmid GC Content   0.49
  Reference Plasmid Mobility Type   conjugative





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0157094 ELLPOFOG_00033 36413 77 Gut 0.92 protein_coding upstream_gene_variant MODIFIER -4979A>C None
M0157095 ELLPOFOG_00040 37226 67 Gut 0.80 protein_coding synonymous_variant LOW 276T>C Tyr92Tyr
M0157096 ELLPOFOG_00040 37233 67 Gut 0.80 protein_coding stop_gained HIGH 283G>T Gly95*
M0157097 ELLPOFOG_00040 37235 67 Gut 0.80 protein_coding synonymous_variant LOW 285A>G Gly95Gly
M0157098 ELLPOFOG_00040 37238 67 Gut 0.80 protein_coding stop_gained HIGH 288G>A Trp96*
M0157099 ELLPOFOG_00040 37241 67 Gut 0.80 protein_coding synonymous_variant LOW 291G>A Arg97Arg
M0157100 ELLPOFOG_00040 37253 67 Gut 0.80 protein_coding synonymous_variant LOW 303C>T Ser101Ser
M0157101 ELLPOFOG_00040 37280 67 Gut 0.80 protein_coding synonymous_variant LOW 330G>A Ala110Ala
M0157102 ELLPOFOG_00040 37290 67 Gut 0.80 protein_coding missense_variant MODERATE 340T>G Ser114Ala
M0157103 ELLPOFOG_00040 37301 67 Gut 0.80 protein_coding synonymous_variant LOW 351A>C Ala117Ala
M0157104 ELLPOFOG_00040 37310 67 Gut 0.80 protein_coding synonymous_variant LOW 360T>G Pro120Pro
M0157105 ELLPOFOG_00040 37313 67 Gut 0.80 protein_coding synonymous_variant LOW 363C>T Ser121Ser
M0157106 ELLPOFOG_00040 37326 43 Gut 0.51 protein_coding missense_variant MODERATE 376C>T His126Tyr
M0157107 ELLPOFOG_00040 37366 67 Gut 0.80 protein_coding missense_variant MODERATE 416C>A Thr139Lys
M0157108 ELLPOFOG_00040 37369 67 Gut 0.80 protein_coding missense_variant MODERATE 419A>C Glu140Ala
M0157109 ELLPOFOG_00040 37307 24 Gut 0.29 protein_coding synonymous_variant LOW 357T>C Arg119Arg
M0157110 ELLPOFOG_00039 36844 10 Gut 0.12 protein_coding stop_gained HIGH 91C>T Gln31*
M0157111 ELLPOFOG_00038 36664 3 Gut 0.04 protein_coding synonymous_variant LOW 186G>A Glu62Glu
M0157112 ELLPOFOG_00038 36680 3 Gut 0.04 protein_coding missense_variant MODERATE 202T>C Cys68Arg






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition
ELLPOFOG_00008 VFG035929 Dispersin 88.9 1.2e-204 1 402 1.0 0.995 Others AatD experiment
ELLPOFOG_00009 VFG035923 Dispersin 97.1 1.3e-109 1 209 1.0 1 Others ATP-binding protein AatC experiment
ELLPOFOG_00010 VFG035916 Dispersin 95.3 1.2e-107 1 212 1.0 0.7766 Others AatB experiment
ELLPOFOG_00011 VFG035909 Dispersin 93.4 6e-215 1 412 1.0 1 Others outer membrane protein AatA experiment
ELLPOFOG_00012 VFG035902 Dispersin 95.2 1.7e-190 17 392 0.9592 1 Others permease AatP experiment
ELLPOFOG_00045 VFG034574 Dispersin 94.8 4e-60 1 116 1.0 1 Others dispersin experiment
ELLPOFOG_00008 VFG035925 Dispersin 90.3 4.4e-206 1 402 1.0 0.9975 Others AatD prediction
ELLPOFOG_00009 VFG035919 Dispersin 98.6 5.2e-110 1 209 1.0 1 Others ATP-binding protein AatC prediction
ELLPOFOG_00010 VFG035915 Dispersin 97.2 3.4e-109 1 212 1.0 0.7766 Others AatB prediction
ELLPOFOG_00011 VFG035909 Dispersin 93.4 4.5e-214 1 412 1.0 1 Others outer membrane protein AatA prediction
ELLPOFOG_00012 VFG035902 Dispersin 95.2 1.2e-189 17 392 0.9592 1 Others permease AatP prediction
ELLPOFOG_00020 VFG042454 Hda adhesin 90.6 1.7e-87 1 170 1.0 1 Adherence HdaA prediction
ELLPOFOG_00022 VFG042452 Hda adhesin 99.5 0 1 800 1.0 0.9501 Adherence HdaC prediction
ELLPOFOG_00023 VFG042451 Hda adhesin 99.6 1.4e-133 26 252 0.9008 1 Adherence HdaD prediction
ELLPOFOG_00027 VFG033809 Vat 99.8 0 1 1364 1.0 1 Effector delivery system vacuolating autotransporter toxin prediction
ELLPOFOG_00032 VFG013084 MsbB2 96.8 2.3e-161 1 279 1.0 0.8885 Others lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase prediction
ELLPOFOG_00033 VFG020186 VirK 99.1 3.2e-188 1 316 1.0 1 Others virulence factor VirK prediction
ELLPOFOG_00037 VFG036086 AggR 97 4e-139 1 265 1.0 1 Regulation aggregative adherence transcriptional regulator AggR prediction
ELLPOFOG_00045 VFG034574 Dispersin 94.8 3e-59 1 116 1.0 1 Others dispersin prediction







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant
ELLPOFOG_00034 PHI:10400 Sfgtr4 (ORF186) 96.1 6.6e-209 1 362 1.0000 1.0000 primates shigellosis putative lipopolysaccharides (LPS) glycosyltransferase (Gtr) reduced virulence
ELLPOFOG_00037 PHI:9507 aggR 97 1.4e-139 1 265 1.0000 1.0000 moths diarrhea transcriptional activator reduced virulence






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
ELLPOFOG_00034 CBG27776.1|GT4 100 3.79e-272 1 362 1 1
ELLPOFOG_00096 BAF33961.1|GH23 100 2.02e-123 1 169 1 1





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term