Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C376
  Reference Plasmid   NZ_AP023421.1
  Reference Plasmid Size   503999
  Reference Plasmid GC Content   0.54
  Reference Plasmid Mobility Type   conjugative





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0163600 KGFBHLND_00339 324171 3 Gut 0.05 protein_coding upstream_gene_variant MODIFIER -4483A>G None
M0163601 KGFBHLND_00516 489304 3 Gut 0.05 protein_coding missense_variant MODERATE 385T>C Trp129Arg
M0163602 KGFBHLND_00040 46017 3 Gut 0.05 protein_coding upstream_gene_variant MODIFIER -1176A>G None
M0163603 KGFBHLND_00042 47056 3 Gut 0.05 protein_coding synonymous_variant LOW 667T>C Leu223Leu
M0163604 KGFBHLND_00195 196740 3 Gut 0.05 protein_coding synonymous_variant LOW 162G>A Arg54Arg
M0163605 KGFBHLND_00195 197757 3 Gut 0.05 protein_coding synonymous_variant LOW 1179T>A Pro393Pro
M0163606 KGFBHLND_00337 317253 4 Gut 0.07 protein_coding missense_variant MODERATE 1520T>C Met507Thr
M0163607 KGFBHLND_00510 482227 3 Gut 0.05 protein_coding synonymous_variant LOW 90T>G Arg30Arg
M0163608 KGFBHLND_00510 482230 3 Gut 0.05 protein_coding synonymous_variant LOW 93A>C Leu31Leu
M0163609 KGFBHLND_00510 482248 3 Gut 0.05 protein_coding synonymous_variant LOW 111A>G Ala37Ala
M0163610 KGFBHLND_00510 483007 3 Gut 0.05 protein_coding synonymous_variant LOW 870T>C Asp290Asp






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism
KGFBHLND_00346 ARO:3002629 72.9 2.06e-158 1 280 0.9894 0.9825 aminoglycoside antibiotic ANT(6) antibiotic inactivation






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
KGFBHLND_00023 BCK85659.1|CE4 100 7.42e-184 1 268 1 1
KGFBHLND_00106 BCK85742.1|GH77 100 0 1 499 1 1
KGFBHLND_00321 BCK85948.1|GH25 100 0 1 550 1 1
KGFBHLND_00356 BCK85983.1|GT26 100 3.42e-170 1 239 1 1
KGFBHLND_00403 BCK86030.1|GT2 100 1.25e-232 1 318 1 1





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
KGFBHLND_00410 3.A.2.2.2 73.5 3e-198 1 457 0.9892 0.9956 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.2 The H+- or Na+-translocating F-type, V-type and A-type ATPase (F-ATPase) Superfamily