Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C405
  Reference Plasmid   NZ_CP007066.1
  Reference Plasmid Size   15921
  Reference Plasmid GC Content   0.28
  Reference Plasmid Mobility Type   non-mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0163791 EKBLAIKO_00002 4665 3 Gut 0.75 protein_coding missense_variant MODERATE 3976G>C Val1326Leu
M0163792 EKBLAIKO_00002 4939 3 Gut 0.75 protein_coding missense_variant MODERATE 4250T>C Val1417Ala
M0163793 EKBLAIKO_00002 4943 3 Gut 0.75 protein_coding synonymous_variant LOW 4254C>T Ser1418Ser
M0163794 EKBLAIKO_00002 4948 3 Gut 0.75 protein_coding missense_variant MODERATE 4259T>G Val1420Gly
M0163795 EKBLAIKO_00005 5980 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -87G>A None
M0163796 EKBLAIKO_00005 6018 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -49T>C None
M0163797 EKBLAIKO_00005 6065 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -2G>A None
M0163798 EKBLAIKO_00005 6071 3 Gut 0.75 protein_coding missense_variant MODERATE 5G>T Ser2Ile
M0163799 EKBLAIKO_00005 6074 3 Gut 0.75 protein_coding missense_variant MODERATE 8T>C Ile3Thr
M0163800 EKBLAIKO_00005 6096 3 Gut 0.75 protein_coding synonymous_variant LOW 30A>G Thr10Thr
M0163801 EKBLAIKO_00005 6097 3 Gut 0.75 protein_coding missense_variant MODERATE 31G>A Gly11Arg
M0163802 EKBLAIKO_00005 6104 3 Gut 0.75 protein_coding missense_variant MODERATE 38T>G Ile13Arg
M0163803 EKBLAIKO_00005 6105 3 Gut 0.75 protein_coding missense_variant MODERATE 39A>G Ile13Met
M0163804 EKBLAIKO_00005 6107 3 Gut 0.75 protein_coding missense_variant MODERATE 41C>T Ser14Leu
M0163805 EKBLAIKO_00005 6111 3 Gut 0.75 protein_coding missense_variant MODERATE 45A>T Leu15Phe
M0163806 EKBLAIKO_00005 6115 3 Gut 0.75 protein_coding missense_variant MODERATE 49A>G Lys17Glu
M0163807 EKBLAIKO_00005 6116 3 Gut 0.75 protein_coding missense_variant MODERATE 50A>T Lys17Ile
M0163808 EKBLAIKO_00005 6156 3 Gut 0.75 protein_coding missense_variant MODERATE 90T>A Phe30Leu
M0163809 EKBLAIKO_00005 6167 3 Gut 0.75 protein_coding missense_variant MODERATE 101A>C Gln34Pro
M0163810 EKBLAIKO_00005 6169 3 Gut 0.75 protein_coding missense_variant MODERATE 103T>G Leu35Val
M0163811 EKBLAIKO_00005 6170 3 Gut 0.75 protein_coding missense_variant MODERATE 104T>C Leu35Ser
M0163812 EKBLAIKO_00005 6187 3 Gut 0.75 protein_coding missense_variant MODERATE 121G>A Ala41Thr
M0163813 EKBLAIKO_00005 6191 3 Gut 0.75 protein_coding missense_variant MODERATE 125A>G Asn42Ser
M0163814 EKBLAIKO_00005 6202 3 Gut 0.75 protein_coding missense_variant MODERATE 136A>C Lys46Gln
M0163815 EKBLAIKO_00005 6217 3 Gut 0.75 protein_coding missense_variant MODERATE 151A>G Asn51Asp
M0163816 EKBLAIKO_00005 6222 3 Gut 0.75 protein_coding synonymous_variant LOW 156A>G Gly52Gly
M0163817 EKBLAIKO_00005 6390 3 Gut 0.75 protein_coding synonymous_variant LOW 324C>T Ala108Ala
M0163818 EKBLAIKO_00005 6720 3 Gut 0.75 protein_coding synonymous_variant LOW 654T>C Ile218Ile
M0163819 EKBLAIKO_00005 6729 3 Gut 0.75 protein_coding synonymous_variant LOW 663T>C Asn221Asn
M0163820 EKBLAIKO_00005 6750 3 Gut 0.75 protein_coding synonymous_variant LOW 684G>A Glu228Glu
M0163821 EKBLAIKO_00005 6787 3 Gut 0.75 protein_coding missense_variant MODERATE 721A>G Arg241Gly
M0163822 EKBLAIKO_00005 6792 3 Gut 0.75 protein_coding synonymous_variant LOW 726G>A Glu242Glu
M0163823 EKBLAIKO_00005 6796 3 Gut 0.75 protein_coding missense_variant MODERATE 730G>A Glu244Lys
M0163824 EKBLAIKO_00005 6798 3 Gut 0.75 protein_coding synonymous_variant LOW 732G>A Glu244Glu
M0163825 EKBLAIKO_00005 6799 3 Gut 0.75 protein_coding missense_variant MODERATE 733G>A Gly245Arg
M0163826 EKBLAIKO_00005 6875 3 Gut 0.75 protein_coding missense_variant MODERATE 809A>C Glu270Ala
M0163827 EKBLAIKO_00005 6900 3 Gut 0.75 protein_coding splice_region_variant&stop_retained_variant LOW 834A>G Ter278Ter
M0163828 EKBLAIKO_00006 6922 3 Gut 0.75 protein_coding synonymous_variant LOW 9T>A Gly3Gly
M0163829 EKBLAIKO_00006 6928 3 Gut 0.75 protein_coding synonymous_variant LOW 15G>A Ala5Ala
M0163830 EKBLAIKO_00006 6949 3 Gut 0.75 protein_coding synonymous_variant LOW 36A>G Lys12Lys
M0163831 EKBLAIKO_00006 6952 3 Gut 0.75 protein_coding synonymous_variant LOW 39C>T Asp13Asp
M0163832 EKBLAIKO_00006 6979 3 Gut 0.75 protein_coding synonymous_variant LOW 66T>A Val22Val
M0163833 EKBLAIKO_00006 7016 3 Gut 0.75 protein_coding missense_variant MODERATE 103A>G Thr35Ala
M0163834 EKBLAIKO_00006 7017 3 Gut 0.75 protein_coding missense_variant MODERATE 104C>A Thr35Asn
M0163835 EKBLAIKO_00006 7021 3 Gut 0.75 protein_coding synonymous_variant LOW 108G>A Thr36Thr
M0163836 EKBLAIKO_00006 7051 3 Gut 0.75 protein_coding synonymous_variant LOW 138T>C Asp46Asp
M0163837 EKBLAIKO_00006 7075 3 Gut 0.75 protein_coding synonymous_variant LOW 162C>T Ile54Ile
M0163838 EKBLAIKO_00006 7086 3 Gut 0.75 protein_coding missense_variant MODERATE 173A>C Lys58Thr
M0163839 EKBLAIKO_00006 7094 3 Gut 0.75 protein_coding missense_variant MODERATE 181A>G Arg61Gly
M0163840 EKBLAIKO_00006 7095 3 Gut 0.75 protein_coding missense_variant MODERATE 182G>A Arg61Lys
M0163841 EKBLAIKO_00006 7163 3 Gut 0.75 protein_coding missense_variant MODERATE 250A>G Asn84Asp
M0163842 EKBLAIKO_00006 7192 3 Gut 0.75 protein_coding synonymous_variant LOW 279A>G Gln93Gln
M0163843 EKBLAIKO_00006 7248 3 Gut 0.75 protein_coding missense_variant MODERATE 335G>A Arg112His
M0163844 EKBLAIKO_00006 7264 3 Gut 0.75 protein_coding synonymous_variant LOW 351A>T Thr117Thr
M0163845 EKBLAIKO_00006 7268 3 Gut 0.75 protein_coding missense_variant MODERATE 355T>G Ser119Ala
M0163846 EKBLAIKO_00006 7291 3 Gut 0.75 protein_coding missense_variant MODERATE 378G>A Met126Ile
M0163847 EKBLAIKO_00006 7315 3 Gut 0.75 protein_coding synonymous_variant LOW 402T>C Ser134Ser
M0163848 EKBLAIKO_00006 7348 3 Gut 0.75 protein_coding synonymous_variant LOW 435T>G Gly145Gly
M0163849 EKBLAIKO_00006 7463 3 Gut 0.75 protein_coding missense_variant MODERATE 550G>C Glu184Gln
M0163850 EKBLAIKO_00006 7483 3 Gut 0.75 protein_coding synonymous_variant LOW 570A>G Lys190Lys






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term