Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C465
  Reference Plasmid   NZ_CP018939.1
  Reference Plasmid Size   73970
  Reference Plasmid GC Content   0.32
  Reference Plasmid Mobility Type   conjugative





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0166332 FKEINLGO_00034 20422 4 Gut 0.15 protein_coding missense_variant MODERATE 613A>G Thr205Ala
M0166333 FKEINLGO_00029 22797 3 Gut 0.12 protein_coding upstream_gene_variant MODIFIER -3907G>A None
M0166334 FKEINLGO_00029 23855 3 Gut 0.12 protein_coding upstream_gene_variant MODIFIER -4965T>C None
M0166335 FKEINLGO_00052 33207 4 Gut 0.15 protein_coding missense_variant MODERATE 629G>A Gly210Glu
M0166336 FKEINLGO_00049 33861 4 Gut 0.15 protein_coding upstream_gene_variant MODIFIER -3413C>T None
M0166337 FKEINLGO_00050 35484 4 Gut 0.15 protein_coding upstream_gene_variant MODIFIER -3798T>C None
M0166338 FKEINLGO_00050 35492 4 Gut 0.15 protein_coding upstream_gene_variant MODIFIER -3806G>A None
M0166339 FKEINLGO_00050 35496 4 Gut 0.15 protein_coding upstream_gene_variant MODIFIER -3810A>G None
M0166340 FKEINLGO_00050 35497 4 Gut 0.15 protein_coding upstream_gene_variant MODIFIER -3811T>A None
M0166341 FKEINLGO_00050 35502 4 Gut 0.15 protein_coding upstream_gene_variant MODIFIER -3816G>A None
M0166342 FKEINLGO_00050 35507 4 Gut 0.15 protein_coding upstream_gene_variant MODIFIER -3821G>A None
M0166343 FKEINLGO_00050 35509 4 Gut 0.15 protein_coding upstream_gene_variant MODIFIER -3823A>G None
M0166344 FKEINLGO_00050 35524 4 Gut 0.15 protein_coding upstream_gene_variant MODIFIER -3838T>C None
M0166345 FKEINLGO_00050 35525 4 Gut 0.15 protein_coding upstream_gene_variant MODIFIER -3839T>A None
M0166346 FKEINLGO_00050 35528 4 Gut 0.15 protein_coding upstream_gene_variant MODIFIER -3842T>C None
M0166347 FKEINLGO_00063 41378 4 Gut 0.15 protein_coding missense_variant MODERATE 323A>G Lys108Arg
M0166348 FKEINLGO_00065 42841 5 Gut 0.19 protein_coding synonymous_variant LOW 447A>G Gln149Gln
M0166349 FKEINLGO_00065 42945 4 Gut 0.15 protein_coding missense_variant MODERATE 343A>G Ile115Val
M0166350 FKEINLGO_00066 43974 5 Gut 0.19 protein_coding missense_variant MODERATE 878G>A Ser293Asn
M0166351 FKEINLGO_00066 44239 4 Gut 0.15 protein_coding missense_variant MODERATE 613G>A Asp205Asn
M0166352 FKEINLGO_00067 45319 4 Gut 0.15 protein_coding missense_variant MODERATE 254C>A Pro85Gln
M0166353 FKEINLGO_00066 49772 3 Gut 0.12 protein_coding upstream_gene_variant MODIFIER -4921G>C None
M0166354 FKEINLGO_00077 51029 5 Gut 0.19 protein_coding missense_variant MODERATE 149A>G Asn50Ser
M0166355 FKEINLGO_00086 57250 4 Gut 0.15 protein_coding synonymous_variant LOW 315A>G Gly105Gly
M0166356 FKEINLGO_00095 63461 5 Gut 0.19 protein_coding synonymous_variant LOW 228T>C Phe76Phe
M0166357 FKEINLGO_00095 63483 5 Gut 0.19 protein_coding missense_variant MODERATE 250G>A Val84Ile
M0166358 FKEINLGO_00035 21066 3 Gut 0.12 protein_coding synonymous_variant LOW 1548G>A Gly516Gly
M0166359 FKEINLGO_00035 21176 3 Gut 0.12 protein_coding missense_variant MODERATE 1438A>G Asn480Asp
M0166360 FKEINLGO_00087 58022 3 Gut 0.12 protein_coding synonymous_variant LOW 219T>C Ser73Ser
M0166361 FKEINLGO_00035 21922 3 Gut 0.12 protein_coding missense_variant MODERATE 692G>A Arg231Gln
M0166362 FKEINLGO_00035 22353 3 Gut 0.12 protein_coding synonymous_variant LOW 261G>A Leu87Leu
M0166363 FKEINLGO_00029 22741 3 Gut 0.12 protein_coding upstream_gene_variant MODIFIER -3851A>G None
M0166364 FKEINLGO_00029 22954 3 Gut 0.12 protein_coding upstream_gene_variant MODIFIER -4064C>T None
M0166365 FKEINLGO_00029 22955 3 Gut 0.12 protein_coding upstream_gene_variant MODIFIER -4065T>C None
M0166366 FKEINLGO_00036 23110 3 Gut 0.12 protein_coding synonymous_variant LOW 294G>A Ala98Ala
M0166367 FKEINLGO_00036 23120 3 Gut 0.12 protein_coding missense_variant MODERATE 284G>A Arg95Lys
M0166368 FKEINLGO_00056 37537 3 Gut 0.12 protein_coding synonymous_variant LOW 867A>C Thr289Thr
M0166369 FKEINLGO_00056 37843 3 Gut 0.12 protein_coding synonymous_variant LOW 561A>G Gly187Gly
M0166370 FKEINLGO_00056 37864 3 Gut 0.12 protein_coding synonymous_variant LOW 540T>C Tyr180Tyr
M0166371 FKEINLGO_00056 37873 3 Gut 0.12 protein_coding synonymous_variant LOW 531A>G Lys177Lys
M0166372 FKEINLGO_00056 37895 3 Gut 0.12 protein_coding missense_variant MODERATE 509A>T Tyr170Phe
M0166373 FKEINLGO_00056 37908 3 Gut 0.12 protein_coding missense_variant MODERATE 496G>A Val166Ile
M0166374 FKEINLGO_00056 37912 3 Gut 0.12 protein_coding synonymous_variant LOW 492T>C Asp164Asp
M0166375 FKEINLGO_00056 37930 3 Gut 0.12 protein_coding synonymous_variant LOW 474T>C Ser158Ser
M0166376 FKEINLGO_00056 37939 3 Gut 0.12 protein_coding synonymous_variant LOW 465A>G Glu155Glu
M0166377 FKEINLGO_00056 38329 3 Gut 0.12 protein_coding synonymous_variant LOW 75G>A Glu25Glu
M0166378 FKEINLGO_00060 39569 3 Gut 0.12 protein_coding missense_variant MODERATE 230T>C Val77Ala
M0166379 FKEINLGO_00062 40699 3 Gut 0.12 protein_coding synonymous_variant LOW 298T>C Leu100Leu
M0166380 FKEINLGO_00066 43649 3 Gut 0.12 protein_coding synonymous_variant LOW 1203T>C Thr401Thr
M0166381 FKEINLGO_00066 44156 3 Gut 0.12 protein_coding synonymous_variant LOW 696C>T Ala232Ala
M0166382 FKEINLGO_00067 44979 3 Gut 0.12 protein_coding synonymous_variant LOW 594C>T Asn198Asn
M0166383 FKEINLGO_00067 45495 3 Gut 0.12 protein_coding synonymous_variant LOW 78A>G Glu26Glu
M0166384 FKEINLGO_00066 49821 3 Gut 0.12 protein_coding upstream_gene_variant MODIFIER -4970C>T None
M0166385 FKEINLGO_00076 49887 3 Gut 0.12 protein_coding missense_variant MODERATE 629G>A Cys210Tyr
M0166386 FKEINLGO_00076 49888 3 Gut 0.12 protein_coding missense_variant MODERATE 628T>A Cys210Ser
M0166387 FKEINLGO_00076 49889 3 Gut 0.12 protein_coding synonymous_variant LOW 627T>A Ile209Ile
M0166388 FKEINLGO_00076 49890 3 Gut 0.12 protein_coding missense_variant MODERATE 626T>C Ile209Thr
M0166389 FKEINLGO_00076 49898 3 Gut 0.12 protein_coding missense_variant MODERATE 618G>A Met206Ile
M0166390 FKEINLGO_00076 49979 3 Gut 0.12 protein_coding synonymous_variant LOW 537G>A Thr179Thr
M0166391 FKEINLGO_00076 50075 3 Gut 0.12 protein_coding synonymous_variant LOW 441A>C Val147Val
M0166392 FKEINLGO_00076 50081 3 Gut 0.12 protein_coding synonymous_variant LOW 435T>C Asp145Asp
M0166393 FKEINLGO_00076 50105 3 Gut 0.12 protein_coding synonymous_variant LOW 411A>G Lys137Lys
M0166394 FKEINLGO_00076 50180 3 Gut 0.12 protein_coding synonymous_variant LOW 336T>G Ser112Ser
M0166395 FKEINLGO_00076 50254 3 Gut 0.12 protein_coding missense_variant MODERATE 262A>T Ser88Cys
M0166396 FKEINLGO_00076 50285 3 Gut 0.12 protein_coding synonymous_variant LOW 231A>G Lys77Lys
M0166397 FKEINLGO_00076 50305 3 Gut 0.12 protein_coding synonymous_variant LOW 211C>T Leu71Leu
M0166398 FKEINLGO_00076 50490 3 Gut 0.12 protein_coding missense_variant MODERATE 26T>C Val9Ala
M0166399 FKEINLGO_00076 50494 3 Gut 0.12 protein_coding synonymous_variant LOW 22C>T Leu8Leu
M0166400 FKEINLGO_00077 50872 3 Gut 0.12 protein_coding synonymous_variant LOW 306C>T Ser102Ser
M0166401 FKEINLGO_00077 50876 3 Gut 0.12 protein_coding missense_variant MODERATE 302A>G Asn101Ser
M0166402 FKEINLGO_00077 50926 3 Gut 0.12 protein_coding missense_variant MODERATE 252A>T Lys84Asn
M0166403 FKEINLGO_00077 50950 3 Gut 0.12 protein_coding synonymous_variant LOW 228A>T Val76Val
M0166404 FKEINLGO_00077 50975 3 Gut 0.12 protein_coding missense_variant MODERATE 203A>G Asn68Ser
M0166405 FKEINLGO_00077 51052 3 Gut 0.12 protein_coding synonymous_variant LOW 126G>A Val42Val
M0166406 FKEINLGO_00078 51336 3 Gut 0.12 protein_coding synonymous_variant LOW 348C>A Gly116Gly
M0166407 FKEINLGO_00078 51423 3 Gut 0.12 protein_coding synonymous_variant LOW 261T>C Gly87Gly
M0166408 FKEINLGO_00078 51501 3 Gut 0.12 protein_coding synonymous_variant LOW 183C>T Ala61Ala
M0166409 FKEINLGO_00078 51543 3 Gut 0.12 protein_coding synonymous_variant LOW 141A>G Glu47Glu
M0166410 FKEINLGO_00092 61436 3 Gut 0.12 protein_coding missense_variant MODERATE 275C>T Ser92Leu
M0166411 FKEINLGO_00092 61439 3 Gut 0.12 protein_coding missense_variant MODERATE 272T>C Leu91Ser
M0166412 FKEINLGO_00092 61480 3 Gut 0.12 protein_coding synonymous_variant LOW 231G>A Val77Val
M0166413 FKEINLGO_00092 61492 3 Gut 0.12 protein_coding synonymous_variant LOW 219T>C Gly73Gly
M0166414 FKEINLGO_00092 61522 3 Gut 0.12 protein_coding synonymous_variant LOW 189T>A Ala63Ala
M0166415 FKEINLGO_00092 61672 3 Gut 0.12 protein_coding missense_variant MODERATE 39C>A Phe13Leu
M0166416 FKEINLGO_00092 61701 3 Gut 0.12 protein_coding missense_variant MODERATE 10A>T Ile4Phe
M0166417 FKEINLGO_00092 61702 3 Gut 0.12 protein_coding synonymous_variant LOW 9G>A Lys3Lys
M0166418 FKEINLGO_00093 61747 3 Gut 0.12 protein_coding synonymous_variant LOW 327G>A Val109Val
M0166419 FKEINLGO_00093 61789 3 Gut 0.12 protein_coding synonymous_variant LOW 285G>A Val95Val
M0166420 FKEINLGO_00093 61792 3 Gut 0.12 protein_coding synonymous_variant LOW 282A>T Pro94Pro
M0166421 FKEINLGO_00093 61804 3 Gut 0.12 protein_coding synonymous_variant LOW 270A>G Thr90Thr
M0166422 FKEINLGO_00093 61822 3 Gut 0.12 protein_coding synonymous_variant LOW 252T>C Ile84Ile
M0166423 FKEINLGO_00093 61873 3 Gut 0.12 protein_coding synonymous_variant LOW 201A>G Glu67Glu
M0166424 FKEINLGO_00093 61923 3 Gut 0.12 protein_coding missense_variant MODERATE 151G>C Ala51Pro
M0166425 FKEINLGO_00086 62080 3 Gut 0.12 protein_coding upstream_gene_variant MODIFIER -4516C>T None
M0166426 FKEINLGO_00094 62291 3 Gut 0.12 protein_coding synonymous_variant LOW 75A>G Glu25Glu
M0166427 FKEINLGO_00094 62294 3 Gut 0.12 protein_coding synonymous_variant LOW 78C>T Ala26Ala
M0166428 FKEINLGO_00094 62684 3 Gut 0.12 protein_coding synonymous_variant LOW 468T>C Arg156Arg
M0166429 FKEINLGO_00094 62756 3 Gut 0.12 protein_coding synonymous_variant LOW 540G>A Leu180Leu
M0166430 FKEINLGO_00094 62768 3 Gut 0.12 protein_coding synonymous_variant LOW 552A>G Leu184Leu
M0166431 FKEINLGO_00094 62777 3 Gut 0.12 protein_coding synonymous_variant LOW 561A>G Glu187Glu
M0166432 FKEINLGO_00094 62783 3 Gut 0.12 protein_coding synonymous_variant LOW 567A>T Val189Val
M0166433 FKEINLGO_00094 62785 3 Gut 0.12 protein_coding missense_variant MODERATE 569G>T Arg190Ile
M0166434 FKEINLGO_00095 63287 4 Gut 0.15 protein_coding synonymous_variant LOW 54A>G Glu18Glu
M0166435 FKEINLGO_00095 63383 4 Gut 0.15 protein_coding synonymous_variant LOW 150T>C Thr50Thr
M0166436 FKEINLGO_00095 63395 4 Gut 0.15 protein_coding synonymous_variant LOW 162A>C Gly54Gly
M0166437 FKEINLGO_00095 63398 4 Gut 0.15 protein_coding synonymous_variant LOW 165C>T Asn55Asn
M0166438 FKEINLGO_00095 63449 4 Gut 0.15 protein_coding synonymous_variant LOW 216T>A Val72Val
M0166439 FKEINLGO_00095 63487 4 Gut 0.15 protein_coding missense_variant MODERATE 254A>G Asn85Ser
M0166440 FKEINLGO_00095 63507 4 Gut 0.15 protein_coding missense_variant MODERATE 274G>T Ala92Ser
M0166441 FKEINLGO_00095 63512 4 Gut 0.15 protein_coding synonymous_variant LOW 279C>T Phe93Phe
M0166442 FKEINLGO_00095 63584 4 Gut 0.15 protein_coding missense_variant MODERATE 351C>A Asn117Lys
M0166443 FKEINLGO_00095 63633 4 Gut 0.15 protein_coding missense_variant MODERATE 400A>C Lys134Gln
M0166444 FKEINLGO_00095 63640 4 Gut 0.15 protein_coding missense_variant MODERATE 407T>A Phe136Tyr
M0166445 FKEINLGO_00095 63641 4 Gut 0.15 protein_coding synonymous_variant LOW 408C>T Phe136Phe
M0166446 FKEINLGO_00095 63643 4 Gut 0.15 protein_coding missense_variant MODERATE 410A>C Gln137Pro
M0166447 FKEINLGO_00095 63668 4 Gut 0.15 protein_coding synonymous_variant LOW 435C>T Asn145Asn
M0166448 FKEINLGO_00095 63683 4 Gut 0.15 protein_coding synonymous_variant LOW 450T>C Tyr150Tyr
M0166449 FKEINLGO_00095 63701 4 Gut 0.15 protein_coding missense_variant MODERATE 468A>G Ile156Met
M0166450 FKEINLGO_00095 63707 4 Gut 0.15 protein_coding synonymous_variant LOW 474G>A Lys158Lys
M0166451 FKEINLGO_00095 63722 4 Gut 0.15 protein_coding synonymous_variant LOW 489A>C Gly163Gly
M0166452 FKEINLGO_00095 63725 4 Gut 0.15 protein_coding missense_variant MODERATE 492G>T Glu164Asp
M0166453 FKEINLGO_00095 63731 4 Gut 0.15 protein_coding synonymous_variant LOW 498G>A Gln166Gln
M0166454 FKEINLGO_00095 63762 4 Gut 0.15 protein_coding missense_variant MODERATE 529T>C Ser177Pro
M0166455 FKEINLGO_00095 63785 4 Gut 0.15 protein_coding missense_variant MODERATE 552G>T Lys184Asn
M0166456 FKEINLGO_00095 63787 4 Gut 0.15 protein_coding missense_variant MODERATE 554A>G Asn185Ser
M0166457 FKEINLGO_00095 63803 4 Gut 0.15 protein_coding synonymous_variant LOW 570T>C Cys190Cys
M0166458 FKEINLGO_00095 63815 4 Gut 0.15 protein_coding synonymous_variant LOW 582G>A Thr194Thr
M0166459 FKEINLGO_00095 63816 4 Gut 0.15 protein_coding missense_variant MODERATE 583C>A Leu195Ile
M0166460 FKEINLGO_00095 63818 4 Gut 0.15 protein_coding synonymous_variant LOW 585T>G Leu195Leu
M0166461 FKEINLGO_00095 63820 4 Gut 0.15 protein_coding missense_variant MODERATE 587C>A Ser196Tyr
M0166462 FKEINLGO_00095 63830 4 Gut 0.15 protein_coding missense_variant MODERATE 597T>A Ser199Arg
M0166463 FKEINLGO_00095 63856 4 Gut 0.15 protein_coding missense_variant MODERATE 623G>A Ser208Asn
M0166464 FKEINLGO_00095 63862 4 Gut 0.15 protein_coding missense_variant MODERATE 629C>T Thr210Ile
M0166465 FKEINLGO_00095 63906 3 Gut 0.12 protein_coding missense_variant MODERATE 673G>A Gly225Ser
M0166466 FKEINLGO_00095 63935 3 Gut 0.12 protein_coding synonymous_variant LOW 702T>C Ser234Ser
M0166467 FKEINLGO_00095 63950 3 Gut 0.12 protein_coding synonymous_variant LOW 717T>C Asn239Asn
M0166468 FKEINLGO_00095 63956 3 Gut 0.12 protein_coding synonymous_variant LOW 723A>G Leu241Leu
M0166469 FKEINLGO_00095 63960 3 Gut 0.12 protein_coding synonymous_variant LOW 727C>A Arg243Arg
M0166470 FKEINLGO_00095 63978 3 Gut 0.12 protein_coding missense_variant MODERATE 745A>G Asn249Asp
M0166471 FKEINLGO_00095 63983 3 Gut 0.12 protein_coding synonymous_variant LOW 750T>C Asp250Asp
M0166472 FKEINLGO_00095 63986 3 Gut 0.12 protein_coding synonymous_variant LOW 753A>G Lys251Lys
M0166473 FKEINLGO_00095 64013 3 Gut 0.12 protein_coding synonymous_variant LOW 780A>T Val260Val
M0166474 FKEINLGO_00095 64058 3 Gut 0.12 protein_coding synonymous_variant LOW 825T>C Ser275Ser
M0166475 FKEINLGO_00095 64091 3 Gut 0.12 protein_coding synonymous_variant LOW 858A>G Leu286Leu
M0166476 FKEINLGO_00095 64092 3 Gut 0.12 protein_coding missense_variant MODERATE 859A>C Lys287Gln






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
FKEINLGO_00064 9.B.154.1.1 92 5.1e-79 4 166 0.9819 0.9760 9 Incompletely Characterized Transport Systems 9.B Putative transport proteins 9.B.154 The Putative Holin-2 (PH-2) Family