Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C467
  Reference Plasmid   NZ_CP019690.1
  Reference Plasmid Size   59559
  Reference Plasmid GC Content   0.42
  Reference Plasmid Mobility Type   conjugative





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0166483 DBBLGHHE_00005 1501 3 Gut 0.75 protein_coding missense_variant MODERATE 21G>T Trp7Cys
M0166484 DBBLGHHE_00005 1508 3 Gut 0.75 protein_coding missense_variant MODERATE 28A>C Ser10Arg
M0166485 DBBLGHHE_00005 1522 3 Gut 0.75 protein_coding stop_gained HIGH 42T>G Tyr14*
M0166486 DBBLGHHE_00005 1605 4 Gut 1.00 protein_coding missense_variant MODERATE 125T>G Phe42Cys
M0166487 DBBLGHHE_00006 2164 4 Gut 1.00 protein_coding upstream_gene_variant MODIFIER -74A>T None
M0166488 DBBLGHHE_00007 2413 4 Gut 1.00 protein_coding synonymous_variant LOW 27G>A Glu9Glu
M0166489 DBBLGHHE_00008 2599 4 Gut 1.00 protein_coding synonymous_variant LOW 6T>C Phe2Phe
M0166490 DBBLGHHE_00010 8756 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -4762A>G None
M0166491 DBBLGHHE_00021 9324 4 Gut 1.00 protein_coding synonymous_variant LOW 321T>C Arg107Arg
M0166492 DBBLGHHE_00022 10811 4 Gut 1.00 protein_coding synonymous_variant LOW 1470C>T Gly490Gly
M0166493 DBBLGHHE_00022 10988 4 Gut 1.00 protein_coding synonymous_variant LOW 1647C>T Ala549Ala
M0166494 DBBLGHHE_00022 11018 4 Gut 1.00 protein_coding synonymous_variant LOW 1677G>T Ser559Ser
M0166495 DBBLGHHE_00022 11204 4 Gut 1.00 protein_coding synonymous_variant LOW 1863A>T Ile621Ile
M0166496 DBBLGHHE_00022 11363 4 Gut 1.00 protein_coding synonymous_variant LOW 2022G>A Glu674Glu
M0166497 DBBLGHHE_00022 11382 4 Gut 1.00 protein_coding missense_variant MODERATE 2041T>A Ser681Thr
M0166498 DBBLGHHE_00022 11978 4 Gut 1.00 protein_coding synonymous_variant LOW 2637T>C Asn879Asn
M0166499 DBBLGHHE_00022 12077 3 Gut 0.75 protein_coding synonymous_variant LOW 2736G>A Gly912Gly
M0166500 DBBLGHHE_00022 12104 3 Gut 0.75 protein_coding synonymous_variant LOW 2763C>T Ile921Ile
M0166501 DBBLGHHE_00022 12108 3 Gut 0.75 protein_coding missense_variant MODERATE 2767A>G Ser923Gly
M0166502 DBBLGHHE_00022 12109 3 Gut 0.75 protein_coding missense_variant MODERATE 2768G>A Ser923Asn
M0166503 DBBLGHHE_00022 12110 3 Gut 0.75 protein_coding synonymous_variant LOW 2769T>C Ser923Ser
M0166504 DBBLGHHE_00022 12137 4 Gut 1.00 protein_coding synonymous_variant LOW 2796T>C Asp932Asp
M0166505 DBBLGHHE_00022 12498 3 Gut 0.75 protein_coding missense_variant MODERATE 3157A>G Ile1053Val
M0166506 DBBLGHHE_00022 12900 4 Gut 1.00 protein_coding synonymous_variant LOW 3559C>A Arg1187Arg
M0166507 DBBLGHHE_00022 13586 3 Gut 0.75 protein_coding synonymous_variant LOW 4245C>T His1415His
M0166508 DBBLGHHE_00023 13652 3 Gut 0.75 protein_coding missense_variant MODERATE 421A>G Ile141Val
M0166509 DBBLGHHE_00023 13729 3 Gut 0.75 protein_coding missense_variant MODERATE 344T>C Ile115Thr
M0166510 DBBLGHHE_00023 13730 3 Gut 0.75 protein_coding missense_variant MODERATE 343A>T Ile115Leu
M0166511 DBBLGHHE_00023 13762 3 Gut 0.75 protein_coding missense_variant MODERATE 311C>T Thr104Ile
M0166512 DBBLGHHE_00023 13815 4 Gut 1.00 protein_coding missense_variant MODERATE 258T>G Asp86Glu
M0166513 DBBLGHHE_00023 13898 3 Gut 0.75 protein_coding missense_variant MODERATE 175T>G Phe59Val
M0166514 DBBLGHHE_00023 13961 4 Gut 1.00 protein_coding missense_variant MODERATE 112A>G Ile38Val
M0166515 DBBLGHHE_00023 13967 4 Gut 1.00 protein_coding missense_variant MODERATE 106T>G Cys36Gly
M0166516 DBBLGHHE_00023 13985 4 Gut 1.00 protein_coding missense_variant MODERATE 88T>G Phe30Val
M0166517 DBBLGHHE_00023 13998 4 Gut 1.00 protein_coding synonymous_variant LOW 75A>C Leu25Leu
M0166518 DBBLGHHE_00023 14595 4 Gut 1.00 protein_coding upstream_gene_variant MODIFIER -523T>C None
M0166519 DBBLGHHE_00023 14641 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -569C>T None
M0166520 DBBLGHHE_00023 14662 4 Gut 1.00 protein_coding upstream_gene_variant MODIFIER -590T>C None
M0166521 DBBLGHHE_00023 14671 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -599C>T None
M0166522 DBBLGHHE_00023 14676 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -604A>G None
M0166523 DBBLGHHE_00025 14707 3 Gut 0.75 protein_coding missense_variant MODERATE 143A>G Gln48Arg
M0166524 DBBLGHHE_00025 14755 3 Gut 0.75 protein_coding missense_variant MODERATE 95T>C Leu32Pro
M0166525 DBBLGHHE_00023 15013 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -941T>A None
M0166526 DBBLGHHE_00027 15464 3 Gut 0.75 protein_coding missense_variant MODERATE 74G>A Arg25Lys
M0166527 DBBLGHHE_00027 15513 3 Gut 0.75 protein_coding missense_variant MODERATE 25G>T Ala9Ser
M0166528 DBBLGHHE_00023 15733 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -1661A>C None
M0166529 DBBLGHHE_00028 15844 3 Gut 0.75 protein_coding synonymous_variant LOW 2094G>A Ala698Ala
M0166530 DBBLGHHE_00028 16030 4 Gut 1.00 protein_coding synonymous_variant LOW 1908C>T Cys636Cys
M0166531 DBBLGHHE_00028 17658 4 Gut 1.00 protein_coding missense_variant MODERATE 280A>G Thr94Ala
M0166532 DBBLGHHE_00028 17878 3 Gut 0.75 protein_coding synonymous_variant LOW 60G>T Gly20Gly
M0166533 DBBLGHHE_00028 17902 3 Gut 0.75 protein_coding synonymous_variant LOW 36A>G Ala12Ala
M0166534 DBBLGHHE_00028 17933 3 Gut 0.75 protein_coding missense_variant MODERATE 5G>A Arg2Lys
M0166535 DBBLGHHE_00029 17984 3 Gut 0.75 protein_coding synonymous_variant LOW 534C>T Arg178Arg
M0166536 DBBLGHHE_00029 18026 3 Gut 0.75 protein_coding synonymous_variant LOW 492T>A Leu164Leu
M0166537 DBBLGHHE_00029 18027 3 Gut 0.75 protein_coding missense_variant MODERATE 491T>C Leu164Pro
M0166538 DBBLGHHE_00029 18029 3 Gut 0.75 protein_coding missense_variant MODERATE 489A>T Gln163His
M0166539 DBBLGHHE_00029 18044 3 Gut 0.75 protein_coding synonymous_variant LOW 474G>A Glu158Glu
M0166540 DBBLGHHE_00029 18059 3 Gut 0.75 protein_coding synonymous_variant LOW 459C>T Ala153Ala
M0166541 DBBLGHHE_00029 18068 3 Gut 0.75 protein_coding synonymous_variant LOW 450A>G Gly150Gly
M0166542 DBBLGHHE_00029 18083 3 Gut 0.75 protein_coding synonymous_variant LOW 435C>A Ile145Ile
M0166543 DBBLGHHE_00029 18102 3 Gut 0.75 protein_coding stop_gained HIGH 416G>A Trp139*
M0166544 DBBLGHHE_00029 18103 3 Gut 0.75 protein_coding missense_variant MODERATE 415T>C Trp139Arg
M0166545 DBBLGHHE_00029 18104 3 Gut 0.75 protein_coding missense_variant MODERATE 414G>C Glu138Asp
M0166546 DBBLGHHE_00029 18131 3 Gut 0.75 protein_coding synonymous_variant LOW 387T>C Ala129Ala
M0166547 DBBLGHHE_00029 18229 3 Gut 0.75 protein_coding missense_variant MODERATE 289A>C Met97Leu
M0166548 DBBLGHHE_00023 18624 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -4552A>C None
M0166549 DBBLGHHE_00027 20374 3 Gut 0.75 protein_coding upstream_gene_variant MODIFIER -4837T>C None
M0166550 DBBLGHHE_00031 20411 3 Gut 0.75 protein_coding synonymous_variant LOW 213C>T Ser71Ser
M0166551 DBBLGHHE_00031 20418 3 Gut 0.75 protein_coding missense_variant MODERATE 206C>T Thr69Ile
M0166552 DBBLGHHE_00031 20609 3 Gut 0.75 protein_coding missense_variant MODERATE 15G>T Leu5Phe
M0166553 DBBLGHHE_00032 20645 3 Gut 0.75 protein_coding synonymous_variant LOW 507T>C Ile169Ile
M0166554 DBBLGHHE_00032 20654 3 Gut 0.75 protein_coding synonymous_variant LOW 498G>A Glu166Glu
M0166555 DBBLGHHE_00032 20916 4 Gut 1.00 protein_coding missense_variant MODERATE 236A>G His79Arg
M0166556 DBBLGHHE_00033 21486 3 Gut 0.75 protein_coding synonymous_variant LOW 366C>T Phe122Phe
M0166557 DBBLGHHE_00034 22401 4 Gut 1.00 protein_coding missense_variant MODERATE 587A>G Asn196Ser
M0166558 DBBLGHHE_00034 22568 4 Gut 1.00 protein_coding synonymous_variant LOW 420T>C Asn140Asn
M0166559 DBBLGHHE_00034 22775 4 Gut 1.00 protein_coding synonymous_variant LOW 213A>C Pro71Pro
M0166560 DBBLGHHE_00034 22901 4 Gut 1.00 protein_coding synonymous_variant LOW 87C>T Gly29Gly






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
DBBLGHHE_00037 ARS09034.1|GH23 100 1.39e-112 1 160 1 1
DBBLGHHE_00043 ARK36115.2|GH23 100 0 1 547 1 1
DBBLGHHE_00054 ARS09050.1|GH23 100 1.73e-134 1 194 1 1
DBBLGHHE_00059 ARK36131.2|GH23 98.1 2.24e-143 1 211 1 1





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
DBBLGHHE_00072 8.A.21.2.2 77.7 2e-119 17 307 0.8954 0.9541 8 Accessory Factors Involved in Transport 8.A Auxiliary transport proteins 8.A.21 The Stomatin/Podocin/Band 7/Nephrosis.2/SPFH (Stomatin) Family