Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C520
  Reference Plasmid   NZ_CP025617.1
  Reference Plasmid Size   86829
  Reference Plasmid GC Content   0.69
  Reference Plasmid Mobility Type   non-mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0093162 KNNEBNJJ_00044 37518 3 Skin 0.05 protein_coding missense_variant MODERATE 58A>G Lys20Glu
M0093163 KNNEBNJJ_00045 37683 3 Skin 0.05 protein_coding upstream_gene_variant MODIFIER -5G>T None
M0093164 KNNEBNJJ_00046 38184 3 Skin 0.05 protein_coding synonymous_variant LOW 90G>A Gly30Gly
M0093165 KNNEBNJJ_00046 38355 3 Skin 0.05 protein_coding synonymous_variant LOW 261A>G Thr87Thr
M0093166 KNNEBNJJ_00046 38463 3 Skin 0.05 protein_coding synonymous_variant LOW 369A>G Leu123Leu
M0093167 KNNEBNJJ_00049 39864 4 Skin 0.06 protein_coding missense_variant MODERATE 191C>T Ala64Val
M0093168 KNNEBNJJ_00052 45042 3 Skin 0.05 protein_coding missense_variant MODERATE 2554G>A Gly852Ser
M0093169 KNNEBNJJ_00053 45259 5 Skin 0.08 protein_coding synonymous_variant LOW 63A>G Ala21Ala
M0093170 KNNEBNJJ_00053 45278 5 Skin 0.08 protein_coding missense_variant MODERATE 82G>A Ala28Thr
M0093171 KNNEBNJJ_00053 45428 3 Skin 0.05 protein_coding missense_variant MODERATE 232A>G Thr78Ala
M0093172 KNNEBNJJ_00053 45612 3 Skin 0.05 protein_coding missense_variant MODERATE 416T>C Val139Ala
M0093173 KNNEBNJJ_00054 46060 3 Skin 0.05 protein_coding missense_variant MODERATE 113C>G Ala38Gly
M0093174 KNNEBNJJ_00054 46165 4 Skin 0.06 protein_coding missense_variant MODERATE 218C>T Thr73Ile
M0093175 KNNEBNJJ_00055 47068 4 Skin 0.06 protein_coding missense_variant MODERATE 481A>G Lys161Glu
M0093176 KNNEBNJJ_00055 47073 4 Skin 0.06 protein_coding synonymous_variant LOW 486T>C Val162Val
M0093177 KNNEBNJJ_00055 47138 3 Skin 0.05 protein_coding missense_variant MODERATE 551T>G Ile184Ser
M0093178 KNNEBNJJ_00056 47694 3 Skin 0.05 protein_coding synonymous_variant LOW 456T>C Tyr152Tyr
M0093179 KNNEBNJJ_00056 47736 3 Skin 0.05 protein_coding synonymous_variant LOW 498C>A Arg166Arg
M0093180 KNNEBNJJ_00058 48400 4 Skin 0.06 protein_coding synonymous_variant LOW 75T>C Pro25Pro
M0093181 KNNEBNJJ_00058 48599 5 Skin 0.08 protein_coding missense_variant MODERATE 274G>A Ala92Thr
M0093182 KNNEBNJJ_00058 48967 4 Skin 0.06 protein_coding synonymous_variant LOW 642T>C Leu214Leu
M0093183 KNNEBNJJ_00058 49023 4 Skin 0.06 protein_coding missense_variant MODERATE 698T>C Val233Ala
M0093184 KNNEBNJJ_00058 49036 4 Skin 0.06 protein_coding synonymous_variant LOW 711A>G Lys237Lys
M0093185 KNNEBNJJ_00058 49246 3 Skin 0.05 protein_coding synonymous_variant LOW 921C>T Ala307Ala
M0093186 KNNEBNJJ_00058 49493 4 Skin 0.06 protein_coding missense_variant MODERATE 1168G>A Val390Ile
M0093187 KNNEBNJJ_00058 49764 4 Skin 0.06 protein_coding missense_variant MODERATE 1439A>G Asp480Gly
M0093188 KNNEBNJJ_00058 49769 4 Skin 0.06 protein_coding missense_variant MODERATE 1444T>C Phe482Leu
M0093189 KNNEBNJJ_00059 50261 5 Skin 0.08 protein_coding synonymous_variant LOW 174G>A Leu58Leu
M0093190 KNNEBNJJ_00059 50282 5 Skin 0.08 protein_coding synonymous_variant LOW 195T>C Ser65Ser
M0093191 KNNEBNJJ_00059 50288 5 Skin 0.08 protein_coding synonymous_variant LOW 201G>A Ala67Ala
M0093192 KNNEBNJJ_00059 50297 5 Skin 0.08 protein_coding synonymous_variant LOW 210C>T Arg70Arg
M0093193 KNNEBNJJ_00059 50309 4 Skin 0.06 protein_coding synonymous_variant LOW 222G>C Ala74Ala
M0093194 KNNEBNJJ_00059 50333 4 Skin 0.06 protein_coding synonymous_variant LOW 246C>A Arg82Arg
M0093195 KNNEBNJJ_00059 50342 4 Skin 0.06 protein_coding synonymous_variant LOW 255C>A Arg85Arg
M0093196 KNNEBNJJ_00059 50345 5 Skin 0.08 protein_coding synonymous_variant LOW 258A>T Gly86Gly
M0093197 KNNEBNJJ_00059 50371 5 Skin 0.08 protein_coding missense_variant MODERATE 284G>A Arg95Lys
M0093198 KNNEBNJJ_00059 50441 4 Skin 0.06 protein_coding synonymous_variant LOW 354G>C Arg118Arg
M0093199 KNNEBNJJ_00051 41445 3 Skin 0.05 protein_coding missense_variant MODERATE 314C>T Thr105Ile
M0093200 KNNEBNJJ_00050 40856 3 Skin 0.05 protein_coding synonymous_variant LOW 156T>C Tyr52Tyr
M0093201 KNNEBNJJ_00052 42812 3 Skin 0.05 protein_coding synonymous_variant LOW 324C>T Ala108Ala
M0093202 KNNEBNJJ_00052 43133 3 Skin 0.05 protein_coding synonymous_variant LOW 645T>C Thr215Thr
M0093203 KNNEBNJJ_00052 43676 3 Skin 0.05 protein_coding synonymous_variant LOW 1188C>T Ala396Ala
M0093204 KNNEBNJJ_00052 44021 4 Skin 0.06 protein_coding synonymous_variant LOW 1533C>T Asp511Asp
M0093205 KNNEBNJJ_00052 44581 4 Skin 0.06 protein_coding missense_variant MODERATE 2093T>C Leu698Pro
M0093206 KNNEBNJJ_00039 33091 5 Skin 0.08 protein_coding missense_variant MODERATE 568A>G Ile190Val
M0093207 KNNEBNJJ_00039 33112 3 Skin 0.05 protein_coding missense_variant MODERATE 589C>G Gln197Glu
M0093208 KNNEBNJJ_00039 33156 3 Skin 0.05 protein_coding synonymous_variant LOW 633C>T Ala211Ala
M0093209 KNNEBNJJ_00041 34219 5 Skin 0.08 protein_coding synonymous_variant LOW 174T>G Gly58Gly
M0093210 KNNEBNJJ_00041 34280 5 Skin 0.08 protein_coding missense_variant MODERATE 235A>G Thr79Ala
M0093211 KNNEBNJJ_00041 34631 5 Skin 0.08 protein_coding missense_variant MODERATE 586A>G Thr196Ala
M0093212 KNNEBNJJ_00041 35061 4 Skin 0.06 protein_coding missense_variant MODERATE 1016G>A Gly339Asp
M0093213 KNNEBNJJ_00041 35428 4 Skin 0.06 protein_coding synonymous_variant LOW 1383T>C Gly461Gly
M0093214 KNNEBNJJ_00042 35759 4 Skin 0.06 protein_coding missense_variant MODERATE 134A>G Glu45Gly
M0093215 KNNEBNJJ_00042 35816 4 Skin 0.06 protein_coding missense_variant MODERATE 191C>T Ala64Val
M0093216 KNNEBNJJ_00042 36046 4 Skin 0.06 protein_coding missense_variant MODERATE 421C>G His141Asp
M0093217 KNNEBNJJ_00042 36397 3 Skin 0.05 protein_coding synonymous_variant LOW 772T>C Leu258Leu
M0093218 KNNEBNJJ_00043 36598 3 Skin 0.05 protein_coding missense_variant MODERATE 110C>T Thr37Ile
M0093219 KNNEBNJJ_00031 27449 3 Skin 0.05 protein_coding synonymous_variant LOW 285C>T Asp95Asp
M0093220 KNNEBNJJ_00031 27476 5 Skin 0.08 protein_coding synonymous_variant LOW 258T>C Val86Val
M0093221 KNNEBNJJ_00031 27701 3 Skin 0.05 protein_coding synonymous_variant LOW 33G>A Glu11Glu
M0093222 KNNEBNJJ_00032 27883 3 Skin 0.05 protein_coding synonymous_variant LOW 75G>C Arg25Arg
M0093223 KNNEBNJJ_00026 28126 3 Skin 0.05 protein_coding upstream_gene_variant MODIFIER -4892T>G None
M0093224 KNNEBNJJ_00033 28577 3 Skin 0.05 protein_coding synonymous_variant LOW 358C>A Arg120Arg
M0093225 KNNEBNJJ_00033 28597 3 Skin 0.05 protein_coding synonymous_variant LOW 378T>C Ala126Ala
M0093226 KNNEBNJJ_00033 28819 3 Skin 0.05 protein_coding synonymous_variant LOW 600C>T Asp200Asp
M0093227 KNNEBNJJ_00028 29297 4 Skin 0.06 protein_coding upstream_gene_variant MODIFIER -3512A>G None
M0093228 KNNEBNJJ_00034 29457 3 Skin 0.05 protein_coding missense_variant MODERATE 33C>A Asp11Glu
M0093229 KNNEBNJJ_00034 29481 3 Skin 0.05 protein_coding synonymous_variant LOW 57G>A Ser19Ser
M0093230 KNNEBNJJ_00031 32450 3 Skin 0.05 protein_coding upstream_gene_variant MODIFIER -4717T>G None
M0093231 KNNEBNJJ_00041 34657 4 Skin 0.06 protein_coding synonymous_variant LOW 612G>T Pro204Pro
M0093232 KNNEBNJJ_00031 31938 4 Skin 0.06 protein_coding upstream_gene_variant MODIFIER -4205G>A None
M0093233 KNNEBNJJ_00031 31988 4 Skin 0.06 protein_coding upstream_gene_variant MODIFIER -4255T>C None
M0093234 KNNEBNJJ_00031 32022 3 Skin 0.05 protein_coding upstream_gene_variant MODIFIER -4289G>C None
M0093235 KNNEBNJJ_00031 32059 3 Skin 0.05 protein_coding upstream_gene_variant MODIFIER -4326T>C None
M0093236 KNNEBNJJ_00031 27485 4 Skin 0.06 protein_coding synonymous_variant LOW 249C>G Thr83Thr
M0093237 KNNEBNJJ_00034 29952 4 Skin 0.06 protein_coding synonymous_variant LOW 528C>A Ala176Ala
M0093238 KNNEBNJJ_00034 29958 4 Skin 0.06 protein_coding synonymous_variant LOW 534C>T His178His
M0093239 KNNEBNJJ_00034 30018 4 Skin 0.06 protein_coding synonymous_variant LOW 594C>T Val198Val
M0093240 KNNEBNJJ_00034 30088 3 Skin 0.05 protein_coding synonymous_variant LOW 664A>C Arg222Arg
M0093241 KNNEBNJJ_00034 30102 3 Skin 0.05 protein_coding synonymous_variant LOW 678C>T Leu226Leu
M0093242 KNNEBNJJ_00034 30128 3 Skin 0.05 protein_coding missense_variant MODERATE 704G>A Arg235His
M0093243 KNNEBNJJ_00028 30332 4 Skin 0.06 protein_coding upstream_gene_variant MODIFIER -4547T>C None
M0093244 KNNEBNJJ_00028 30340 4 Skin 0.06 protein_coding upstream_gene_variant MODIFIER -4555G>T None
M0093245 KNNEBNJJ_00035 30759 3 Skin 0.05 protein_coding missense_variant MODERATE 217T>G Ser73Ala
M0093246 KNNEBNJJ_00035 30851 4 Skin 0.06 protein_coding missense_variant MODERATE 125T>C Val42Ala
M0093247 KNNEBNJJ_00035 30859 4 Skin 0.06 protein_coding synonymous_variant LOW 117A>G Thr39Thr
M0093248 KNNEBNJJ_00035 30903 4 Skin 0.06 protein_coding missense_variant MODERATE 73A>G Thr25Ala
M0093249 KNNEBNJJ_00036 30983 4 Skin 0.06 protein_coding synonymous_variant LOW 666T>C Asp222Asp
M0093250 KNNEBNJJ_00036 31166 3 Skin 0.05 protein_coding synonymous_variant LOW 483T>C Arg161Arg
M0093251 KNNEBNJJ_00036 31451 3 Skin 0.05 protein_coding synonymous_variant LOW 198T>C Ser66Ser
M0093252 KNNEBNJJ_00058 49850 3 Skin 0.05 protein_coding missense_variant MODERATE 1525T>G Ser509Ala
M0093253 KNNEBNJJ_00058 49852 3 Skin 0.05 protein_coding synonymous_variant LOW 1527C>T Ser509Ser
M0093254 KNNEBNJJ_00058 49882 3 Skin 0.05 protein_coding synonymous_variant LOW 1557T>C Gly519Gly
M0093255 KNNEBNJJ_00059 50294 4 Skin 0.06 protein_coding synonymous_variant LOW 207G>A Arg69Arg
M0093256 KNNEBNJJ_00059 50543 3 Skin 0.05 protein_coding synonymous_variant LOW 456T>C Arg152Arg
M0093257 KNNEBNJJ_00081 67039 3 Skin 0.05 protein_coding missense_variant MODERATE 496A>G Met166Val
M0093258 KNNEBNJJ_00076 63461 4 Skin 0.06 protein_coding missense_variant MODERATE 49G>A Ala17Thr
M0093259 KNNEBNJJ_00076 63462 3 Skin 0.05 protein_coding missense_variant MODERATE 50C>T Ala17Val






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
KNNEBNJJ_00024 AWD25880.1|GH184 100 0 1 588 1 1
CKPNNMCB_00024 AWD25880.1|GH184 100 0 1 588 1 1





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term