Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C523
  Reference Plasmid   NZ_CP025846.1
  Reference Plasmid Size   86624
  Reference Plasmid GC Content   0.49
  Reference Plasmid Mobility Type   conjugative





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0167626 HKPBKONN_00034 26525 3 Gut 0.07 protein_coding synonymous_variant LOW 297T>C Ala99Ala
M0167627 HKPBKONN_00027 20039 3 Gut 0.07 protein_coding synonymous_variant LOW 108G>C Ala36Ala
M0167628 HKPBKONN_00027 20042 3 Gut 0.07 protein_coding synonymous_variant LOW 111A>G Thr37Thr
M0167629 HKPBKONN_00028 20163 3 Gut 0.07 protein_coding synonymous_variant LOW 210A>G Arg70Arg
M0167630 HKPBKONN_00028 20178 3 Gut 0.07 protein_coding synonymous_variant LOW 195A>G Lys65Lys
M0167631 HKPBKONN_00028 20223 3 Gut 0.07 protein_coding synonymous_variant LOW 150T>A Ala50Ala
M0167632 HKPBKONN_00020 20404 3 Gut 0.07 protein_coding upstream_gene_variant MODIFIER -4115A>G None
M0167633 HKPBKONN_00020 20479 3 Gut 0.07 protein_coding upstream_gene_variant MODIFIER -4190T>C None
M0167634 HKPBKONN_00020 20484 3 Gut 0.07 protein_coding upstream_gene_variant MODIFIER -4195G>A None
M0167635 HKPBKONN_00020 20486 3 Gut 0.07 protein_coding upstream_gene_variant MODIFIER -4197C>T None
M0167636 HKPBKONN_00020 20530 3 Gut 0.07 protein_coding upstream_gene_variant MODIFIER -4241G>A None
M0167637 HKPBKONN_00020 20532 3 Gut 0.07 protein_coding upstream_gene_variant MODIFIER -4243A>G None
M0167638 HKPBKONN_00029 21182 3 Gut 0.07 protein_coding synonymous_variant LOW 231G>A Lys77Lys
M0167639 HKPBKONN_00029 21377 3 Gut 0.07 protein_coding synonymous_variant LOW 426T>C Ser142Ser
M0167640 HKPBKONN_00029 21396 3 Gut 0.07 protein_coding missense_variant MODERATE 445A>C Lys149Gln
M0167641 HKPBKONN_00036 27347 3 Gut 0.07 protein_coding missense_variant MODERATE 1909C>T Pro637Ser






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition
HKPBKONN_00051 VFG042382 EtpA 96.5 0 1 598 1.0 0.9917 Adherence two-partner secretion transporter EtpB experiment
HKPBKONN_00051 VFG042382 EtpA 96.5 0 1 598 1.0 0.9917 Adherence two-partner secretion transporter EtpB prediction







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
HKPBKONN_00049 CBJ04457.1|GT41 98.9 0 1 637 1 1





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
HKPBKONN_00036 3.A.7.10.1 99 0 1 760 1.0000 7.9479 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.7 The Type IV (Conjugal DNA-Protein Transfer or VirB) Secretory Pathway (IVSP) Family
HKPBKONN_00037 3.A.7.10.1 96.3 2.5e-200 1 356 1.0000 3.7083 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.7 The Type IV (Conjugal DNA-Protein Transfer or VirB) Secretory Pathway (IVSP) Family
HKPBKONN_00038 3.A.7.10.1 99 2.3e-242 1 402 1.0000 4.1875 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.7 The Type IV (Conjugal DNA-Protein Transfer or VirB) Secretory Pathway (IVSP) Family
HKPBKONN_00048 9.B.127.1.3 94.5 7e-89 1 164 1.0000 1.0000 9 Incompletely Characterized Transport Systems 9.B Putative transport proteins 9.B.127 The DUF2919 (PF11143) Family
HKPBKONN_00051 1.B.20.3.3 96.5 0 1 598 1.0000 0.9917 1 Channels/Pores 1.B β-Barrel Porins 1.B.20 The Two-Partner Secretion (TPS) Family