Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C529
  Reference Plasmid   NZ_CP026049.1
  Reference Plasmid Size   206855
  Reference Plasmid GC Content   0.53
  Reference Plasmid Mobility Type   conjugative





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0093260 FGFHOOBC_00099 80914 3 Skin 0.16 protein_coding upstream_gene_variant MODIFIER -2957G>A None
M0093261 FGFHOOBC_00106 81216 3 Skin 0.16 protein_coding stop_lost&splice_region_variant HIGH 298T>C Ter100Argext*?
M0093262 FGFHOOBC_00142 109722 4 Skin 0.21 protein_coding missense_variant MODERATE 800C>T Ala267Val
M0093263 FGFHOOBC_00144 111068 3 Skin 0.16 protein_coding synonymous_variant LOW 432C>G Val144Val
M0093264 FGFHOOBC_00147 114585 4 Skin 0.21 protein_coding synonymous_variant LOW 519A>C Ala173Ala
M0093265 FGFHOOBC_00148 115573 3 Skin 0.16 protein_coding synonymous_variant LOW 552C>T Thr184Thr






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition
FGFHOOBC_00083 VFG034679 Ibes 71.9 5.2e-183 1 459 0.9957 0.9978 Invasion Cu(+)/Ag(+) efflux RND transporter outer membrane channel CusC experiment
NDIIDGBF_00083 VFG034679 Ibes 71.9 5.2e-183 1 459 0.9957 0.9978 Invasion Cu(+)/Ag(+) efflux RND transporter outer membrane channel CusC experiment
FGFHOOBC_00083 VFG034652 Ibes 71.9 3e-182 1 459 0.9957 0.9978 Invasion Cu(+)/Ag(+) efflux RND transporter outer membrane channel CusC prediction
NDIIDGBF_00083 VFG034652 Ibes 71.9 3e-182 1 459 0.9957 0.9978 Invasion Cu(+)/Ag(+) efflux RND transporter outer membrane channel CusC prediction







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group
FGFHOOBC_00080 Silver (Ag) 80.4 1.6e-61 1 143 1.0000 1.0000 experiment
FGFHOOBC_00081 Silver (Ag) 92.7 1.3e-253 1 490 1.0061 0.9940 experiment
FGFHOOBC_00082 Silver (Ag) 92.5 1.3e-115 1 226 1.0088 1.0000 experiment
FGFHOOBC_00083 Silver (Ag) 97.6 1e-250 1 461 1.0000 1.0000 experiment
FGFHOOBC_00084 Silver (Ag) 98.9 3.1e-47 28 117 0.7692 0.9375 experiment
FGFHOOBC_00085 Silver (Ag) 98.4 3.2e-243 1 430 1.0000 1.0000 experiment
FGFHOOBC_00086 Silver (Ag) 99.4 0 1 1048 1.0000 1.0000 experiment
FGFHOOBC_00088 Silver (Ag) 98.4 0 1 813 1.0012 0.9879 experiment
FGFHOOBC_00092 Copper (Cu) 100 0 1 605 1.0000 1.0000 experiment
FGFHOOBC_00093 Copper (Cu) 100 1.5e-175 1 296 1.0000 1.0000 experiment
FGFHOOBC_00094 Copper (Cu) 100 4.9e-67 1 126 1.0000 1.0000 experiment
FGFHOOBC_00095 Copper (Cu) 99.7 2.5e-165 1 309 1.0000 1.0000 experiment
FGFHOOBC_00096 Copper (Cu) 100 1.7e-126 1 226 1.0000 1.0000 experiment
FGFHOOBC_00097 Copper (Cu) 99.4 1.5e-262 1 466 1.0000 1.0000 experiment
FGFHOOBC_00098 Copper (Cu), Silver (Ag) 89.6 1e-63 1 144 1.0000 1.0000 experiment
FGFHOOBC_00123 Arsenic (As), Antimony (Sb), Bismuth (Bi) 100 8.1e-64 1 117 1.0000 1.0000 experiment
FGFHOOBC_00124 Arsenic (As), Antimony (Sb) 98.3 1.6e-67 1 120 1.0000 1.0000 experiment
FGFHOOBC_00125 Arsenic (As), Antimony (Sb) 99 0 1 583 1.0000 1.0000 experiment
FGFHOOBC_00126 Arsenic (As), Antimony (Sb) 99.5 2e-232 1 429 1.0000 1.0000 experiment
FGFHOOBC_00127 Arsenic (As), Antimony (Sb) 99.3 3.5e-77 1 141 1.0000 1.0000 experiment
FGFHOOBC_00133 Arsenic (As), Antimony (Sb) 84.2 4.9e-182 1 387 0.9974 0.9021 experiment
FGFHOOBC_00134 Arsenic (As), Antimony (Sb) 80 5.8e-64 1 140 0.9859 0.9929 experiment
FGFHOOBC_00135 Arsenic (As) 78.6 7.9e-98 23 240 0.9167 0.9483 experiment
NDIIDGBF_00080 Silver (Ag) 80.4 1.6e-61 1 143 1.0000 1.0000 experiment
NDIIDGBF_00081 Silver (Ag) 92.7 1.3e-253 1 490 1.0061 0.9940 experiment
NDIIDGBF_00082 Silver (Ag) 92.5 1.3e-115 1 226 1.0088 1.0000 experiment
NDIIDGBF_00083 Silver (Ag) 97.6 1e-250 1 461 1.0000 1.0000 experiment
NDIIDGBF_00084 Silver (Ag) 98.9 3.1e-47 28 117 0.7692 0.9375 experiment
NDIIDGBF_00085 Silver (Ag) 98.4 3.2e-243 1 430 1.0000 1.0000 experiment
NDIIDGBF_00086 Silver (Ag) 99.4 0 1 1048 1.0000 1.0000 experiment
NDIIDGBF_00088 Silver (Ag) 98.4 0 1 813 1.0012 0.9879 experiment
NDIIDGBF_00092 Copper (Cu) 100 0 1 605 1.0000 1.0000 experiment
NDIIDGBF_00093 Copper (Cu) 100 1.5e-175 1 296 1.0000 1.0000 experiment
NDIIDGBF_00094 Copper (Cu) 100 4.9e-67 1 126 1.0000 1.0000 experiment
NDIIDGBF_00095 Copper (Cu) 99.7 2.5e-165 1 309 1.0000 1.0000 experiment
NDIIDGBF_00096 Copper (Cu) 100 1.7e-126 1 226 1.0000 1.0000 experiment
NDIIDGBF_00097 Copper (Cu) 99.4 1.5e-262 1 466 1.0000 1.0000 experiment
NDIIDGBF_00098 Copper (Cu), Silver (Ag) 89.6 1e-63 1 144 1.0000 1.0000 experiment
NDIIDGBF_00123 Arsenic (As), Antimony (Sb), Bismuth (Bi) 100 8.1e-64 1 117 1.0000 1.0000 experiment
NDIIDGBF_00124 Arsenic (As), Antimony (Sb) 98.3 1.6e-67 1 120 1.0000 1.0000 experiment
NDIIDGBF_00125 Arsenic (As), Antimony (Sb) 99 0 1 583 1.0000 1.0000 experiment
NDIIDGBF_00126 Arsenic (As), Antimony (Sb) 99.5 2e-232 1 429 1.0000 1.0000 experiment
NDIIDGBF_00127 Arsenic (As), Antimony (Sb) 99.3 3.5e-77 1 141 1.0000 1.0000 experiment
NDIIDGBF_00133 Arsenic (As), Antimony (Sb) 84.2 4.9e-182 1 387 0.9974 0.9021 experiment
NDIIDGBF_00134 Arsenic (As), Antimony (Sb) 80 5.8e-64 1 140 0.9859 0.9929 experiment
NDIIDGBF_00135 Arsenic (As) 78.6 7.9e-98 23 240 0.9167 0.9483 experiment
FGFHOOBC_00080 Silver (Ag) 83.2 1.3e-61 1 143 1.0000 1.0000 prediction
FGFHOOBC_00081 Silver (Ag) 100 1.8e-275 1 491 1.0000 1.0000 prediction
FGFHOOBC_00082 Silver (Ag) 100 1.7e-124 1 226 1.0000 1.0000 prediction
FGFHOOBC_00083 Silver (Ag) 100 1.6e-254 1 461 1.0000 0.8986 prediction
FGFHOOBC_00084 Silver (Ag) 100 1.6e-60 1 117 1.0000 1.0000 prediction
FGFHOOBC_00085 Silver (Ag) 100 3.2e-244 1 430 1.0000 1.0000 prediction
FGFHOOBC_00086 Silver (Ag) 100 0 1 1048 1.0000 1.0000 prediction
FGFHOOBC_00088 Silver (Ag) 100 0 1 813 1.0000 1.0000 prediction
FGFHOOBC_00092 Copper (Cu) 100 0 1 605 1.0000 0.9967 prediction
FGFHOOBC_00093 Copper (Cu) 100 3.5e-173 1 296 1.0000 0.9900 prediction
FGFHOOBC_00094 Copper (Cu) 100 1.1e-64 1 126 1.0000 1.0000 prediction
FGFHOOBC_00095 Copper (Cu) 100 4.4e-163 1 309 1.0000 1.0000 prediction
FGFHOOBC_00096 Copper (Cu) 100 3.9e-124 1 226 1.0000 0.9741 prediction
FGFHOOBC_00097 Copper (Cu) 100 3.7e-262 1 466 1.0000 1.0000 prediction
FGFHOOBC_00098 Copper (Cu), Silver (Ag) 99.3 6e-70 1 144 1.0000 1.0000 prediction
FGFHOOBC_00123 Arsenic (As) 100 1.9e-61 1 117 1.0000 1.0000 prediction
FGFHOOBC_00124 Arsenic (As) 100 4.4e-66 1 120 1.0000 1.0000 prediction
FGFHOOBC_00125 Arsenic (As), Antimony (Sb) 100 0 1 583 1.0000 1.0000 prediction
FGFHOOBC_00126 Arsenic (As), Antimony (Sb) 99.8 6.9e-231 1 428 0.9977 1.0000 prediction
FGFHOOBC_00127 Arsenic (As) 100 1.6e-75 1 141 1.0000 1.0000 prediction
FGFHOOBC_00133 Arsenic (As), Antimony (Sb) 84 3.5e-181 1 387 0.9974 0.9021 prediction
FGFHOOBC_00134 Arsenic (As) 81.4 5.4e-63 1 140 0.9859 0.9929 prediction
FGFHOOBC_00135 Arsenic (As) 95 3.3e-129 1 240 1.0000 1.0000 prediction
FGFHOOBC_00157 Arsenic (As) 100 1.3e-62 1 117 1.0000 1.0000 prediction
NDIIDGBF_00080 Silver (Ag) 83.2 1.3e-61 1 143 1.0000 1.0000 prediction
NDIIDGBF_00081 Silver (Ag) 100 1.8e-275 1 491 1.0000 1.0000 prediction
NDIIDGBF_00082 Silver (Ag) 100 1.7e-124 1 226 1.0000 1.0000 prediction
NDIIDGBF_00083 Silver (Ag) 100 1.6e-254 1 461 1.0000 0.8986 prediction
NDIIDGBF_00084 Silver (Ag) 100 1.6e-60 1 117 1.0000 1.0000 prediction
NDIIDGBF_00085 Silver (Ag) 100 3.2e-244 1 430 1.0000 1.0000 prediction
NDIIDGBF_00086 Silver (Ag) 100 0 1 1048 1.0000 1.0000 prediction
NDIIDGBF_00088 Silver (Ag) 100 0 1 813 1.0000 1.0000 prediction
NDIIDGBF_00092 Copper (Cu) 100 0 1 605 1.0000 0.9967 prediction
NDIIDGBF_00093 Copper (Cu) 100 3.5e-173 1 296 1.0000 0.9900 prediction
NDIIDGBF_00094 Copper (Cu) 100 1.1e-64 1 126 1.0000 1.0000 prediction
NDIIDGBF_00095 Copper (Cu) 100 4.4e-163 1 309 1.0000 1.0000 prediction
NDIIDGBF_00096 Copper (Cu) 100 3.9e-124 1 226 1.0000 0.9741 prediction
NDIIDGBF_00097 Copper (Cu) 100 3.7e-262 1 466 1.0000 1.0000 prediction
NDIIDGBF_00098 Copper (Cu), Silver (Ag) 99.3 6e-70 1 144 1.0000 1.0000 prediction
NDIIDGBF_00123 Arsenic (As) 100 1.9e-61 1 117 1.0000 1.0000 prediction
NDIIDGBF_00124 Arsenic (As) 100 4.4e-66 1 120 1.0000 1.0000 prediction
NDIIDGBF_00125 Arsenic (As), Antimony (Sb) 100 0 1 583 1.0000 1.0000 prediction
NDIIDGBF_00126 Arsenic (As), Antimony (Sb) 99.8 6.9e-231 1 428 0.9977 1.0000 prediction
NDIIDGBF_00127 Arsenic (As) 100 1.6e-75 1 141 1.0000 1.0000 prediction
NDIIDGBF_00133 Arsenic (As), Antimony (Sb) 84 3.5e-181 1 387 0.9974 0.9021 prediction
NDIIDGBF_00134 Arsenic (As) 81.4 5.4e-63 1 140 0.9859 0.9929 prediction
NDIIDGBF_00135 Arsenic (As) 95 3.3e-129 1 240 1.0000 1.0000 prediction
NDIIDGBF_00157 Arsenic (As) 100 1.3e-62 1 117 1.0000 1.0000 prediction






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant
FGFHOOBC_00153 PHI:6268 lacZ 96.6 0 1 1024 1.0000 1.0000 rodents urinary tract infection beta-galactosidase reduced virulence
NDIIDGBF_00153 PHI:6268 lacZ 96.6 0 1 1024 1.0000 1.0000 rodents urinary tract infection beta-galactosidase reduced virulence






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
FGFHOOBC_00153 AUU07625.1|GH2 100 0 1 1024 1 1
NDIIDGBF_00153 AUU07625.1|GH2 100 0 1 1024 1 1





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
FGFHOOBC_00083 1.B.17.3.4 97.6 3.8e-249 1 461 1.0000 1.0000 1 Channels/Pores 1.B β-Barrel Porins 1.B.17 The Outer Membrane Factor (OMF) Family
FGFHOOBC_00086 2.A.6.1.3 99.4 0 1 1048 1.0000 1.0000 2 Electrochemical Potential-driven Transporters 2.A Porters (uniporters, symporters, antiporters) 2.A.6 The Resistance-Nodulation-Cell Division (RND) Superfamily
FGFHOOBC_00088 3.A.3.5.4 98.4 0 1 813 1.0000 0.9879 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.3 The P-type ATPase (P-ATPase) Superfamily
FGFHOOBC_00090 1.A.34.1.3 99.6 4.3e-135 1 245 1.0000 1.0000 1 Channels/Pores 1.A α-Type Channels 1.A.34 The <i>Bacillus</i> Gap Junction-like Channel-forming Complex (GJ-CC) Family
FGFHOOBC_00093 1.B.76.1.5 100 5.7e-174 1 296 1.0000 1.0000 1 Channels/Pores 1.B β-Barrel Porins 1.B.76 The Copper Resistance Putative Porin (CopB) Family
FGFHOOBC_00095 9.B.62.1.1 99.7 9.5e-164 1 309 1.0000 1.0000 9 Incompletely Characterized Transport Systems 9.B Putative transport proteins 9.B.62 The Copper Resistance (CopD) Family
FGFHOOBC_00125 3.A.4.1.1 85.5 1.5e-282 1 581 0.9966 1.3543 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.4 The Arsenite-Antimonite (ArsAB) Efflux Family
FGFHOOBC_00126 3.A.4.1.1 93.9 1.5e-220 1 429 1.0000 1.0000 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.4 The Arsenite-Antimonite (ArsAB) Efflux Family
FGFHOOBC_00133 3.A.4.1.1 83.2 1.3e-178 1 387 0.9974 0.9021 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.4 The Arsenite-Antimonite (ArsAB) Efflux Family
FGFHOOBC_00152 2.A.1.5.1 99.3 2.1e-230 1 416 0.9976 0.9976 2 Electrochemical Potential-driven Transporters 2.A Porters (uniporters, symporters, antiporters) 2.A.1 The Major Facilitator Superfamily (MFS)
FGFHOOBC_00158 2.A.53.3.1 90.4 1.5e-248 1 492 1.0000 1.0000 2 Electrochemical Potential-driven Transporters 2.A Porters (uniporters, symporters, antiporters) 2.A.53 The Sulfate Permease (SulP) Family
NDIIDGBF_00083 1.B.17.3.4 97.6 3.8e-249 1 461 1.0000 1.0000 1 Channels/Pores 1.B β-Barrel Porins 1.B.17 The Outer Membrane Factor (OMF) Family
NDIIDGBF_00086 2.A.6.1.3 99.4 0 1 1048 1.0000 1.0000 2 Electrochemical Potential-driven Transporters 2.A Porters (uniporters, symporters, antiporters) 2.A.6 The Resistance-Nodulation-Cell Division (RND) Superfamily
NDIIDGBF_00088 3.A.3.5.4 98.4 0 1 813 1.0000 0.9879 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.3 The P-type ATPase (P-ATPase) Superfamily
NDIIDGBF_00090 1.A.34.1.3 99.6 4.3e-135 1 245 1.0000 1.0000 1 Channels/Pores 1.A α-Type Channels 1.A.34 The <i>Bacillus</i> Gap Junction-like Channel-forming Complex (GJ-CC) Family
NDIIDGBF_00093 1.B.76.1.5 100 5.7e-174 1 296 1.0000 1.0000 1 Channels/Pores 1.B β-Barrel Porins 1.B.76 The Copper Resistance Putative Porin (CopB) Family
NDIIDGBF_00095 9.B.62.1.1 99.7 9.5e-164 1 309 1.0000 1.0000 9 Incompletely Characterized Transport Systems 9.B Putative transport proteins 9.B.62 The Copper Resistance (CopD) Family
NDIIDGBF_00125 3.A.4.1.1 85.5 1.5e-282 1 581 0.9966 1.3543 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.4 The Arsenite-Antimonite (ArsAB) Efflux Family
NDIIDGBF_00126 3.A.4.1.1 93.9 1.5e-220 1 429 1.0000 1.0000 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.4 The Arsenite-Antimonite (ArsAB) Efflux Family
NDIIDGBF_00133 3.A.4.1.1 83.2 1.3e-178 1 387 0.9974 0.9021 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.4 The Arsenite-Antimonite (ArsAB) Efflux Family
NDIIDGBF_00152 2.A.1.5.1 99.3 2.1e-230 1 416 0.9976 0.9976 2 Electrochemical Potential-driven Transporters 2.A Porters (uniporters, symporters, antiporters) 2.A.1 The Major Facilitator Superfamily (MFS)
NDIIDGBF_00158 2.A.53.3.1 90.4 1.5e-248 1 492 1.0000 1.0000 2 Electrochemical Potential-driven Transporters 2.A Porters (uniporters, symporters, antiporters) 2.A.53 The Sulfate Permease (SulP) Family