Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C539
  Reference Plasmid   NZ_CP027600.1
  Reference Plasmid Size   120604
  Reference Plasmid GC Content   0.53
  Reference Plasmid Mobility Type   conjugative





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0168104 KFKPOEHG_00078 71010 3 Gut 0.23 protein_coding synonymous_variant LOW 180C>T Thr60Thr
M0168105 KFKPOEHG_00075 72116 3 Gut 0.23 protein_coding upstream_gene_variant MODIFIER -4110C>T None






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition
KFKPOEHG_00027 VFG042365 EspI, SPATE 77.4 0 25 1387 0.9827 1 Effector delivery system hypothetical protein prediction
KFKPOEHG_00122 VFG043980 Colicin Ib 98.9 1.2e-247 1 463 1.0 0.7396 Exotoxin colicin-like pore-forming protein prediction







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group
KFKPOEHG_00108 Mercury (Hg) 78.2 4.9e-33 1 78 1.0000 1.0000 experiment
KFKPOEHG_00109 Mercury (Hg) 82.6 1.3e-48 1 120 1.0083 1.0000 experiment
KFKPOEHG_00110 Mercury (Hg) 89.5 1.3e-229 1 455 1.0000 0.8111 experiment
KFKPOEHG_00113 Mercury (Hg) 90.1 7e-39 1 91 1.0000 1.0000 experiment
KFKPOEHG_00114 Mercury (Hg) 100 1.3e-62 19 134 0.8657 1.0000 experiment
KFKPOEHG_00115 Mercury (Hg) 93.8 4e-73 1 144 1.0000 1.0000 experiment
KFKPOEHG_00108 Mercury (Hg) 83.3 2.7e-32 1 78 1.0000 1.0000 prediction
KFKPOEHG_00109 Mercury (Hg) 91.7 5.5e-53 1 120 1.0000 1.0000 prediction
KFKPOEHG_00110 Mercury (Hg), Phenylmercury Acetate [class: Organo-mercury] 100 1.6e-254 1 455 1.0000 0.8067 prediction
KFKPOEHG_00113 Mercury (Hg) 100 6.3e-41 1 91 1.0000 0.8835 prediction
KFKPOEHG_00114 Mercury (Hg) 90.3 1.2e-64 1 134 1.0000 1.0000 prediction
KFKPOEHG_00115 Mercury (Hg) 100 4.3e-76 1 144 1.0000 0.7619 prediction






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism
KFKPOEHG_00095 ARO:3000873 100 5.12e-208 1 286 1.0000 1.0000 monobactam TEM beta-lactamase antibiotic inactivation
KFKPOEHG_00097 ARO:3002641 98.5 6.49e-208 1 271 1.0000 1.0000 aminoglycoside antibiotic APH(3') antibiotic inactivation
KFKPOEHG_00100 ARO:3002660 100 4.41e-183 1 247 0.9686 0.8885 aminoglycoside antibiotic APH(6) antibiotic inactivation
KFKPOEHG_00101 ARO:3002639 99.6 5.98e-202 1 267 1.0000 1.0000 aminoglycoside antibiotic APH(3'') antibiotic inactivation
KFKPOEHG_00102 ARO:3000412 99.6 8.29e-186 1 271 1.0000 1.0000 sulfonamide antibiotic sulfonamide resistant sul antibiotic target replacement
KFKPOEHG_00118 ARO:3000165 99.7 7.17e-278 1 390 0.9774 0.9198 tetracycline antibiotic major facilitator superfamily (MFS) antibiotic efflux pump antibiotic efflux






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
KFKPOEHG_00070 AKF16924.1|GH23 100 9.46e-114 1 157 1 1





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
KFKPOEHG_00020 3.A.7.10.1 79.8 0 1 724 0.9439 7.5417 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.7 The Type IV (Conjugal DNA-Protein Transfer or VirB) Secretory Pathway (IVSP) Family
KFKPOEHG_00022 3.A.7.10.1 76.2 7e-189 1 399 0.9301 4.1563 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.7 The Type IV (Conjugal DNA-Protein Transfer or VirB) Secretory Pathway (IVSP) Family
KFKPOEHG_00033 1.E.53.1.10 92.9 6.3e-17 9 50 0.8400 0.9767 1 Channels/Pores 1.E Holins 1.E.53 The Toxic Hok/Gef Protein (Hok/Gef) Family
KFKPOEHG_00053 3.A.7.10.1 76.5 2.2e-139 1 324 1.0000 3.4063 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.7 The Type IV (Conjugal DNA-Protein Transfer or VirB) Secretory Pathway (IVSP) Family
KFKPOEHG_00054 3.A.7.10.1 70.3 5.2e-90 6 227 0.9610 2.2917 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.7 The Type IV (Conjugal DNA-Protein Transfer or VirB) Secretory Pathway (IVSP) Family
KFKPOEHG_00055 3.A.7.10.1 74.1 5e-41 1 116 1.0000 1.1979 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.7 The Type IV (Conjugal DNA-Protein Transfer or VirB) Secretory Pathway (IVSP) Family
KFKPOEHG_00057 3.A.7.10.1 71.3 1.3e-39 1 94 0.9792 0.9792 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.7 The Type IV (Conjugal DNA-Protein Transfer or VirB) Secretory Pathway (IVSP) Family
KFKPOEHG_00058 3.A.7.10.1 79 1.1e-177 4 379 0.9843 3.9167 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.7 The Type IV (Conjugal DNA-Protein Transfer or VirB) Secretory Pathway (IVSP) Family
KFKPOEHG_00059 3.A.7.10.1 79 1.3e-124 1 271 0.9748 2.8021 3 Primary Active Transporters 3.A P-P-bond-hydrolysis-driven transporters 3.A.7 The Type IV (Conjugal DNA-Protein Transfer or VirB) Secretory Pathway (IVSP) Family
KFKPOEHG_00108 1.A.72.5.1 100 1.8e-39 1 78 1.0000 1.0000 1 Channels/Pores 1.A α-Type Channels 1.A.72 The Mercuric Ion Pore (Mer) Superfamily
KFKPOEHG_00113 1.A.72.3.1 90.1 2.6e-37 1 91 1.0000 1.0000 1 Channels/Pores 1.A α-Type Channels 1.A.72 The Mercuric Ion Pore (Mer) Superfamily
KFKPOEHG_00114 1.A.72.3.1 87.9 3.5e-54 19 134 0.8657 1.2747 1 Channels/Pores 1.A α-Type Channels 1.A.72 The Mercuric Ion Pore (Mer) Superfamily
KFKPOEHG_00118 2.A.1.2.4 100 2.4e-223 1 399 1.0000 1.0000 2 Electrochemical Potential-driven Transporters 2.A Porters (uniporters, symporters, antiporters) 2.A.1 The Major Facilitator Superfamily (MFS)
KFKPOEHG_00119 2.A.7.3.67 100 1.8e-159 1 294 1.0000 1.0000 2 Electrochemical Potential-driven Transporters 2.A Porters (uniporters, symporters, antiporters) 2.A.7 The Drug/Metabolite Transporter (DMT) Superfamily
KFKPOEHG_00122 1.C.1.1.2 99.1 9.3e-248 1 463 1.0000 0.7396 1 Channels/Pores 1.C Pore-Forming Toxins (Proteins and Peptides) 1.C.1 The Channel-forming Colicin (Colicin) Family
KFKPOEHG_00123 8.B.24.2.1 97.3 1.2e-55 1 110 0.9565 0.8661 8 Accessory Factors Involved in Transport 8.B Ribosomally synthesized protein/peptide toxins/agonists that target channels and carriers 8.B.24 The Colicin Immunity Protein (ColIP) Functional Family