Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C547
  Reference Plasmid   NZ_CP029007.1
  Reference Plasmid Size   149504
  Reference Plasmid GC Content   0.40
  Reference Plasmid Mobility Type   mobilizable





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0093486 NGPKOJKM_00021 14661 3 Skin 0.09 protein_coding synonymous_variant LOW 501T>A Ser167Ser
M0093487 NGPKOJKM_00021 14742 3 Skin 0.09 protein_coding synonymous_variant LOW 420T>C Leu140Leu
M0093488 NGPKOJKM_00021 15032 3 Skin 0.09 protein_coding missense_variant MODERATE 130C>G Gln44Glu
M0093489 NGPKOJKM_00021 15066 3 Skin 0.09 protein_coding synonymous_variant LOW 96A>G Lys32Lys
M0093490 NGPKOJKM_00016 15174 3 Skin 0.09 protein_coding upstream_gene_variant MODIFIER -4701T>G None
M0093491 NGPKOJKM_00022 15416 3 Skin 0.09 protein_coding synonymous_variant LOW 120A>G Glu40Glu
M0093492 NGPKOJKM_00022 15440 3 Skin 0.09 protein_coding synonymous_variant LOW 144C>T Ile48Ile
M0093493 NGPKOJKM_00022 15574 3 Skin 0.09 protein_coding missense_variant MODERATE 278A>G His93Arg
M0093494 NGPKOJKM_00022 15827 3 Skin 0.09 protein_coding synonymous_variant LOW 531C>T Val177Val
M0093495 NGPKOJKM_00022 16077 3 Skin 0.09 protein_coding missense_variant MODERATE 781A>G Lys261Glu
M0093496 NGPKOJKM_00023 16338 3 Skin 0.09 protein_coding missense_variant MODERATE 925T>C Cys309Arg
M0093497 NGPKOJKM_00023 16498 3 Skin 0.09 protein_coding synonymous_variant LOW 765T>G Pro255Pro
M0093498 NGPKOJKM_00023 16830 3 Skin 0.09 protein_coding missense_variant MODERATE 433A>C Asn145His
M0093499 NGPKOJKM_00023 16945 4 Skin 0.13 protein_coding synonymous_variant LOW 318C>T Tyr106Tyr
M0093500 NGPKOJKM_00024 17807 3 Skin 0.09 protein_coding synonymous_variant LOW 441G>A Gln147Gln
M0093501 NGPKOJKM_00024 17843 3 Skin 0.09 protein_coding synonymous_variant LOW 405G>T Thr135Thr
M0093502 NGPKOJKM_00024 17926 3 Skin 0.09 protein_coding missense_variant MODERATE 322T>G Leu108Val
M0093503 NGPKOJKM_00024 17996 3 Skin 0.09 protein_coding synonymous_variant LOW 252T>C Asn84Asn
M0093504 NGPKOJKM_00019 18289 3 Skin 0.09 protein_coding upstream_gene_variant MODIFIER -4959A>G None
M0093505 NGPKOJKM_00020 18408 4 Skin 0.13 protein_coding upstream_gene_variant MODIFIER -4060A>G None
M0093506 NGPKOJKM_00020 18637 4 Skin 0.13 protein_coding upstream_gene_variant MODIFIER -4289A>T None
M0093507 NGPKOJKM_00020 18657 4 Skin 0.13 protein_coding upstream_gene_variant MODIFIER -4309T>A None
M0093508 NGPKOJKM_00025 18887 3 Skin 0.09 protein_coding synonymous_variant LOW 171G>A Leu57Leu
M0093509 NGPKOJKM_00025 18893 3 Skin 0.09 protein_coding synonymous_variant LOW 177A>T Val59Val
M0093510 NGPKOJKM_00026 19025 3 Skin 0.09 protein_coding synonymous_variant LOW 12C>T Arg4Arg
M0093511 NGPKOJKM_00026 19265 3 Skin 0.09 protein_coding synonymous_variant LOW 252A>T Val84Val
M0093512 NGPKOJKM_00026 19523 3 Skin 0.09 protein_coding synonymous_variant LOW 510G>T Gly170Gly
M0093513 NGPKOJKM_00021 20128 3 Skin 0.09 protein_coding upstream_gene_variant MODIFIER -4967G>A None
M0093514 NGPKOJKM_00023 20213 3 Skin 0.09 protein_coding upstream_gene_variant MODIFIER -2951T>G None
M0093515 NGPKOJKM_00027 20489 3 Skin 0.09 protein_coding synonymous_variant LOW 135G>T Val45Val
M0093516 NGPKOJKM_00027 20507 3 Skin 0.09 protein_coding synonymous_variant LOW 153A>G Gly51Gly
M0093517 NGPKOJKM_00027 20508 3 Skin 0.09 protein_coding synonymous_variant LOW 154T>C Leu52Leu
M0093518 NGPKOJKM_00027 20747 3 Skin 0.09 protein_coding synonymous_variant LOW 393G>A Leu131Leu
M0093519 NGPKOJKM_00027 20813 3 Skin 0.09 protein_coding synonymous_variant LOW 459A>C Val153Val
M0093520 NGPKOJKM_00027 20819 3 Skin 0.09 protein_coding synonymous_variant LOW 465A>G Gly155Gly
M0093521 NGPKOJKM_00027 21005 3 Skin 0.09 protein_coding synonymous_variant LOW 651A>G Gln217Gln
M0093522 NGPKOJKM_00023 21212 3 Skin 0.09 protein_coding upstream_gene_variant MODIFIER -3950T>C None
M0093523 NGPKOJKM_00023 21427 3 Skin 0.09 protein_coding upstream_gene_variant MODIFIER -4165A>C None
M0093524 NGPKOJKM_00023 21446 3 Skin 0.09 protein_coding upstream_gene_variant MODIFIER -4184A>G None
M0093525 NGPKOJKM_00023 21491 3 Skin 0.09 protein_coding upstream_gene_variant MODIFIER -4229T>C None
M0093526 NGPKOJKM_00028 21588 3 Skin 0.09 protein_coding synonymous_variant LOW 69T>C Phe23Phe
M0093527 NGPKOJKM_00028 21596 3 Skin 0.09 protein_coding missense_variant MODERATE 77C>G Ala26Gly
M0093528 NGPKOJKM_00028 21648 3 Skin 0.09 protein_coding synonymous_variant LOW 129T>A Leu43Leu
M0093529 NGPKOJKM_00028 21657 3 Skin 0.09 protein_coding synonymous_variant LOW 138T>A Gly46Gly
M0093530 NGPKOJKM_00028 21711 3 Skin 0.09 protein_coding synonymous_variant LOW 192G>A Ala64Ala
M0093531 NGPKOJKM_00028 21777 3 Skin 0.09 protein_coding synonymous_variant LOW 258A>G Pro86Pro
M0093532 NGPKOJKM_00028 21818 3 Skin 0.09 protein_coding missense_variant MODERATE 299C>G Ala100Gly
M0093533 NGPKOJKM_00023 21828 3 Skin 0.09 protein_coding upstream_gene_variant MODIFIER -4566A>G None
M0093534 NGPKOJKM_00023 21851 3 Skin 0.09 protein_coding upstream_gene_variant MODIFIER -4589A>G None
M0093535 NGPKOJKM_00023 21867 3 Skin 0.09 protein_coding upstream_gene_variant MODIFIER -4605A>T None
M0093536 NGPKOJKM_00023 21887 3 Skin 0.09 protein_coding upstream_gene_variant MODIFIER -4625T>C None
M0093537 NGPKOJKM_00023 21891 3 Skin 0.09 protein_coding upstream_gene_variant MODIFIER -4629A>C None
M0093538 NGPKOJKM_00023 21957 3 Skin 0.09 protein_coding upstream_gene_variant MODIFIER -4695T>A None
M0093539 NGPKOJKM_00023 22005 5 Skin 0.16 protein_coding upstream_gene_variant MODIFIER -4743G>A None
M0093540 NGPKOJKM_00023 22110 4 Skin 0.13 protein_coding upstream_gene_variant MODIFIER -4848A>G None
M0093541 NGPKOJKM_00023 22234 4 Skin 0.13 protein_coding upstream_gene_variant MODIFIER -4972A>C None
M0093542 NGPKOJKM_00023 22246 4 Skin 0.13 protein_coding upstream_gene_variant MODIFIER -4984T>G None
M0093543 NGPKOJKM_00023 22250 3 Skin 0.09 protein_coding upstream_gene_variant MODIFIER -4988T>G None
M0093544 NGPKOJKM_00024 22272 3 Skin 0.09 protein_coding upstream_gene_variant MODIFIER -4025T>A None
M0093545 NGPKOJKM_00024 22324 5 Skin 0.16 protein_coding upstream_gene_variant MODIFIER -4077A>G None
M0093546 NGPKOJKM_00024 22341 3 Skin 0.09 protein_coding upstream_gene_variant MODIFIER -4094C>T None
M0093547 NGPKOJKM_00024 22345 5 Skin 0.16 protein_coding upstream_gene_variant MODIFIER -4098G>A None
M0093548 NGPKOJKM_00024 22398 5 Skin 0.16 protein_coding upstream_gene_variant MODIFIER -4151T>C None
M0093549 NGPKOJKM_00024 22404 5 Skin 0.16 protein_coding upstream_gene_variant MODIFIER -4157A>G None
M0093550 NGPKOJKM_00029 22453 5 Skin 0.16 protein_coding synonymous_variant LOW 33T>C Arg11Arg
M0093551 NGPKOJKM_00029 22516 5 Skin 0.16 protein_coding synonymous_variant LOW 96G>A Val32Val
M0093552 NGPKOJKM_00029 22651 6 Skin 0.19 protein_coding synonymous_variant LOW 231C>T Ala77Ala
M0093553 NGPKOJKM_00029 22666 5 Skin 0.16 protein_coding synonymous_variant LOW 246T>A Arg82Arg
M0093554 NGPKOJKM_00029 22678 5 Skin 0.16 protein_coding synonymous_variant LOW 258T>A Ile86Ile
M0093555 NGPKOJKM_00029 22909 5 Skin 0.16 protein_coding synonymous_variant LOW 489T>C Ser163Ser
M0093556 NGPKOJKM_00029 22913 5 Skin 0.16 protein_coding missense_variant MODERATE 493C>T His165Tyr
M0093557 NGPKOJKM_00029 22939 4 Skin 0.13 protein_coding synonymous_variant LOW 519C>G Leu173Leu
M0093558 NGPKOJKM_00029 23003 4 Skin 0.13 protein_coding missense_variant MODERATE 583A>G Ile195Val
M0093559 NGPKOJKM_00029 23224 3 Skin 0.09 protein_coding synonymous_variant LOW 804A>G Gly268Gly
M0093560 NGPKOJKM_00029 23281 5 Skin 0.16 protein_coding missense_variant MODERATE 861T>G Phe287Leu
M0093561 NGPKOJKM_00029 23391 4 Skin 0.13 protein_coding missense_variant MODERATE 971T>C Val324Ala
M0093562 NGPKOJKM_00029 23460 6 Skin 0.19 protein_coding missense_variant MODERATE 1040T>G Val347Gly
M0093563 NGPKOJKM_00029 23532 3 Skin 0.09 protein_coding missense_variant MODERATE 1112C>A Thr371Asn
M0093564 NGPKOJKM_00030 23803 3 Skin 0.09 protein_coding synonymous_variant LOW 135A>G Ser45Ser
M0093565 NGPKOJKM_00030 23839 4 Skin 0.13 protein_coding synonymous_variant LOW 171C>T Ser57Ser
M0093566 NGPKOJKM_00030 23942 3 Skin 0.09 protein_coding missense_variant MODERATE 274A>G Thr92Ala
M0093567 NGPKOJKM_00030 23974 3 Skin 0.09 protein_coding synonymous_variant LOW 306A>G Gly102Gly
M0093568 NGPKOJKM_00034 26904 3 Skin 0.09 protein_coding missense_variant MODERATE 72G>T Leu24Phe
M0093569 NGPKOJKM_00141 130676 3 Skin 0.09 protein_coding synonymous_variant LOW 417A>G Leu139Leu
M0093570 NGPKOJKM_00144 133208 3 Skin 0.09 protein_coding missense_variant MODERATE 313A>C Thr105Pro






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group
NGPKOJKM_00049 Mercury (Hg) 88.7 5.5e-73 1 151 1.0000 1.0000 experiment
NGPKOJKM_00052 Mercury (Hg) 79.4 3.5e-61 1 141 1.0000 1.0000 experiment
NGPKOJKM_00053 Mercury (Hg) 90.9 7.8e-290 1 561 1.0000 1.0000 experiment
NGPKOJKM_00049 Mercury (Hg) 99.3 7.9e-81 1 151 1.0000 1.0000 prediction
NGPKOJKM_00052 Mercury (Hg) 80.1 7.2e-60 1 141 1.0000 1.0000 prediction
NGPKOJKM_00053 Mercury (Hg), Phenylmercury Acetate [class: Organo-mercury] 100 0 1 561 1.0000 1.0000 prediction






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage
NGPKOJKM_00058 QXX86794.1|CE4 100 3e-250 1 320 1 1





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term