Annotation Categories of the Plasmid Cluster







Summary of the plasmid cluster

Basic Information about the Plasmid Cluster

  Cluster Information   Plasmid Cluster ID   C570
  Reference Plasmid   NZ_CP032820.1
  Reference Plasmid Size   51017
  Reference Plasmid GC Content   0.36
  Reference Plasmid Mobility Type   conjugative





Mutation sites in the plasmid cluster


The table lists mutations identified in the plasmid cluster.
Note: Mutations identified in this plasmid cluster are listed below. Click on a mutation ID to view full details..

mutid gname pos count tissue frequnt biotype consequence impact nucchange aachange
M0168578 NFGINMHH_00049 35789 3 Gut 0.07 protein_coding missense_variant MODERATE 712A>G Ser238Gly
M0168579 NFGINMHH_00051 38700 3 Gut 0.07 protein_coding synonymous_variant LOW 96T>C Ser32Ser
M0168580 NFGINMHH_00051 38703 3 Gut 0.07 protein_coding synonymous_variant LOW 99A>G Val33Val
M0168581 NFGINMHH_00017 13511 3 Gut 0.07 protein_coding synonymous_variant LOW 594C>T Pro198Pro
M0168582 NFGINMHH_00029 24964 3 Gut 0.07 protein_coding upstream_gene_variant MODIFIER -2528A>G None
M0168583 NFGINMHH_00029 25801 3 Gut 0.07 protein_coding upstream_gene_variant MODIFIER -3365A>G None
M0168584 NFGINMHH_00029 25810 3 Gut 0.07 protein_coding upstream_gene_variant MODIFIER -3374T>C None
M0168585 NFGINMHH_00029 25832 3 Gut 0.07 protein_coding upstream_gene_variant MODIFIER -3396T>C None
M0168586 NFGINMHH_00029 25835 3 Gut 0.07 protein_coding upstream_gene_variant MODIFIER -3399C>A None
M0168587 NFGINMHH_00005 5466 3 Gut 0.07 protein_coding missense_variant MODERATE 168G>T Lys56Asn
M0168588 NFGINMHH_00028 21638 3 Gut 0.07 protein_coding missense_variant MODERATE 315G>T Glu105Asp
M0168589 NFGINMHH_00029 25569 3 Gut 0.07 protein_coding upstream_gene_variant MODIFIER -3133A>C None
M0168590 NFGINMHH_00029 25570 3 Gut 0.07 protein_coding upstream_gene_variant MODIFIER -3134G>T None
M0168591 NFGINMHH_00029 26564 3 Gut 0.07 protein_coding upstream_gene_variant MODIFIER -4128G>T None
M0168592 NFGINMHH_00038 29334 3 Gut 0.07 protein_coding missense_variant MODERATE 88C>G Leu30Val
M0168593 NFGINMHH_00044 31960 3 Gut 0.07 protein_coding missense_variant MODERATE 974G>A Gly325Glu
M0168594 NFGINMHH_00035 33293 4 Gut 0.09 protein_coding upstream_gene_variant MODIFIER -4697T>C None
M0168595 NFGINMHH_00051 39078 3 Gut 0.07 protein_coding synonymous_variant LOW 474C>T His158His
M0168596 NFGINMHH_00014 11078 3 Gut 0.07 protein_coding synonymous_variant LOW 501T>C Asp167Asp
M0168597 NFGINMHH_00014 11126 3 Gut 0.07 protein_coding synonymous_variant LOW 549T>C Asn183Asn
M0168598 NFGINMHH_00014 11141 3 Gut 0.07 protein_coding synonymous_variant LOW 564T>C Asp188Asp
M0168599 NFGINMHH_00014 11321 3 Gut 0.07 protein_coding synonymous_variant LOW 744G>A Glu248Glu
M0168600 NFGINMHH_00014 11336 3 Gut 0.07 protein_coding synonymous_variant LOW 759A>T Val253Val
M0168601 NFGINMHH_00016 11451 3 Gut 0.07 protein_coding upstream_gene_variant MODIFIER -746C>T None
M0168602 NFGINMHH_00015 11593 3 Gut 0.07 protein_coding synonymous_variant LOW 564A>G Lys188Lys
M0168603 NFGINMHH_00015 11659 3 Gut 0.07 protein_coding synonymous_variant LOW 498C>T Gly166Gly
M0168604 NFGINMHH_00015 11965 3 Gut 0.07 protein_coding synonymous_variant LOW 192A>G Arg64Arg
M0168605 NFGINMHH_00015 12129 3 Gut 0.07 protein_coding missense_variant MODERATE 28A>G Thr10Ala
M0168606 NFGINMHH_00016 12319 3 Gut 0.07 protein_coding synonymous_variant LOW 123T>A Ser41Ser
M0168607 NFGINMHH_00016 12361 3 Gut 0.07 protein_coding synonymous_variant LOW 165T>C Asn55Asn
M0168608 NFGINMHH_00016 12412 3 Gut 0.07 protein_coding synonymous_variant LOW 216T>C Asp72Asp
M0168609 NFGINMHH_00016 12415 3 Gut 0.07 protein_coding synonymous_variant LOW 219C>T Ser73Ser
M0168610 NFGINMHH_00016 12434 3 Gut 0.07 protein_coding missense_variant MODERATE 238G>A Val80Ile
M0168611 NFGINMHH_00016 12450 3 Gut 0.07 protein_coding missense_variant MODERATE 254C>A Pro85Gln
M0168612 NFGINMHH_00016 12472 3 Gut 0.07 protein_coding synonymous_variant LOW 276C>T Asn92Asn
M0168613 NFGINMHH_00016 12478 3 Gut 0.07 protein_coding synonymous_variant LOW 282A>G Lys94Lys
M0168614 NFGINMHH_00016 12511 3 Gut 0.07 protein_coding synonymous_variant LOW 315G>A Glu105Glu
M0168615 NFGINMHH_00016 12547 3 Gut 0.07 protein_coding synonymous_variant LOW 351C>T Leu117Leu
M0168616 NFGINMHH_00016 12598 3 Gut 0.07 protein_coding synonymous_variant LOW 402T>C Ser134Ser
M0168617 NFGINMHH_00016 12667 3 Gut 0.07 protein_coding synonymous_variant LOW 471T>C Gly157Gly
M0168618 NFGINMHH_00017 13178 3 Gut 0.07 protein_coding synonymous_variant LOW 261T>C Phe87Phe
M0168619 NFGINMHH_00021 16953 4 Gut 0.09 protein_coding synonymous_variant LOW 174A>G Arg58Arg
M0168620 NFGINMHH_00021 17253 3 Gut 0.07 protein_coding synonymous_variant LOW 474A>T Gly158Gly
M0168621 NFGINMHH_00020 21729 3 Gut 0.07 protein_coding downstream_gene_variant MODIFIER *4950C>A None
M0168622 NFGINMHH_00031 23875 3 Gut 0.07 protein_coding synonymous_variant LOW 880C>T Leu294Leu
M0168623 NFGINMHH_00041 30156 3 Gut 0.07 protein_coding synonymous_variant LOW 102C>T Ser34Ser
M0168624 NFGINMHH_00041 30157 3 Gut 0.07 protein_coding missense_variant MODERATE 101G>C Ser34Thr
M0168625 NFGINMHH_00041 30249 3 Gut 0.07 protein_coding synonymous_variant LOW 9G>A Ala3Ala
M0168626 NFGINMHH_00041 30250 3 Gut 0.07 protein_coding missense_variant MODERATE 8C>T Ala3Val
M0168627 NFGINMHH_00033 30866 3 Gut 0.07 protein_coding upstream_gene_variant MODIFIER -3117T>G None






Analysis of virulence factors contributing to bacterial pathogenicity


This table presents virulence factors identified within the plasmid cluster.
      Note: Virulence factor analysis was performed using VFDB. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known virulence factors are listed.

Gene Name vf_gene_id vf_name identity evalue qstart qend query_coverage subject_coverage vf_category gene_description condition







        Analysis of biocide and heavy metal resistance genes to assess antimicrobial risk and environmental impact


This table presents biocides and heavy metals resistance genes identified within the plasmid cluster.
      Note: Analyzing biocide and heavy metal resistance genes based on BacMet to evaluate bacterial resistance risk and the potential impact of environmental heavy metal contamination. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, E-value < 1e-5) to known biocide and heavy metal resistance genes are listed.

Gene Name compound identity evalue qstart qend query_coverage subject_coverage group






        Analyzing antimicrobial resistance genes to assess bacterial resistance to antibiotics and other antimicrobial agents


This table presents antimicrobial resistance genes identified within the plasmid cluster.
      Note: Antimicrobial resistance was performed using CARD. Genes in plasmid clusters showing strong homology (identity > 70%, coverage > 70%, E-value < 1e-5) to known antimicrobial resistance genes are listed.

Gene Name aro_accession identity evalue qstart qend query_coverage subject_coverage drug_class amr_gene_family resistance_mechanism
NFGINMHH_00002 ARO:3003002 99.4 2.4e-229 1 320 0.9877 0.9969 cephalosporin CfxA beta-lactamase antibiotic inactivation






Analysis of pathogenicity genes to explore pathogen-host interactions


This table presents host pathogen-host interactions within the plasmid cluster.
      Note: Analyzing pathogenicity-related genes using PHI-base to understand pathogen virulence mechanisms and their impact on host interactions. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e-5) to known pathogenicity-related genes are listed.

Gene Name phi_molconn_id host gene_name identity evalue qstart qend query_coverage subject_coverage host_descripton disease_name function phenotype_of_mutant






        Analyzing carbohydrate-active enzyme genes to uncover mechanisms of nutrient degradation


This table presents carbohydrate-active enzyme genes identified within the plasmid cluster.
      Note: Annotation of carbohydrate-active enzyme genes was performed using CAZy to explore mechanisms of nutrient breakdown and utilization. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known CAZyme genes are listed.

Gene Name cazy_id identity evalue qstart qend query_coverage subject_coverage





        Analyzing transport proteins to understand bacterial strategies for substrate uptake and detoxification


This table presents transport proteins within the plasmid cluster.
      Note: Investigation of transport proteins based on TCDB to uncover bacterial mechanisms of substrate transport and environmental detoxification. Genes in plasmid clusters showing strong homology (identity > 70%, subject coverage > 70%, and E-value < 1e−5) to known transport protein entries are listed.

Gene Name tcid identity evalue qstart qend query_coverage subject_coverage class_field class_term subclass subclass_term family family_term
NFGINMHH_00019 9.B.154.1.1 95.2 3.1e-84 1 167 1.0000 1.0000 9 Incompletely Characterized Transport Systems 9.B Putative transport proteins 9.B.154 The Putative Holin-2 (PH-2) Family